• Title/Summary/Keyword: bound form

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Factors affecting the formation of bound 3-monochloropropane-1,2-diol in a fried snack model (유탕 과자 모델에서 결합형 3-monochloropropane-1,2-diol 생성에 영향을 미치는 요인)

  • Kang, Jun-Hyuk;Joung, Woo-Young;Rho, Hoi-Jin;Baek, Hyung-Hee
    • Korean Journal of Food Science and Technology
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    • v.52 no.6
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    • pp.565-572
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    • 2020
  • The 3-monochloropropane-1,2-diol (3-MCPD) is a contaminant that occurs in foodstuffs in its free form as well as in its bound form. The objective of this study was to evaluate the effects of emulsifier, frying temperature, and the amounts of salt and oil on the formation of bound 3-MCPD in a fried snack model. Emulsifier affected the formation of bound 3-MCPD; furthermore, it was observed that the largest amount of bound 3-MCPD was detected in the fried snack model when glycerin esters of fatty acids were used as emulsifiers. Frying temperature also affected the formation of bound 3-MCPD, which increased significantly as the frying temperature increased from 145 to 190℃. In addition, salt affected the formation of bound 3-MCPD. As the amount of salt increased, the amount of bound 3-MCPD also increased significantly. Moreover, it was observed that the amount of oil did not affect the formation of bound 3-MCPD. These results will aid in the reduction of bound 3-MCPD in fried snacks.

Comparison of the Efficiency of Absorbed Nitrogen Use from Different Protein Sources in Diets Having Similar Amino Acid Balance

  • Lee, K.U.;Boyd, R.D.;Austic, R.E.;Ross, D.A.;Han, In K.
    • Asian-Australasian Journal of Animal Sciences
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    • v.11 no.6
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    • pp.725-731
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    • 1998
  • Nine crossbred female pigs fitted with the bladder catheters were used to investigate the effects of dietary protein form on the efficiency of absorbed nitrogen for nitrogen retention in growing pigs. Combinations of the main protein sources were corn-soybean meal (CSM; slow + slow absorption rate form), corn-hydrolyzed casein (CAS; slow + rapid absorption rate form) and corn-porcine plasma (CPL; slow + intermediate absorption rate form). All experimental diets were formulated to be isonitrogenous (CP 11%) and isocaloric (3.5 Mcal/kg) and synthetic amino acids were added to the diet as required to maintain an equivalent amino acid profile among diets. Fecal digestibility of nitrogen was not different among treatments (p > 0.10). Ingested nitrogen was absorbed with an apparent efficiency of 82% to 84%. Mean nitrogen retention in pigs fed the CSM diet was as high as for pigs fed the CPL diet (0.74 g N/kg $BW^{0.75}$ per d), which was higher than the N retention rate in pigs fed CAS diet (0.68 g/kg $BW^{0.75}$ per d; P < 0.05). Apparent biological values (ABV = 100 ${\times}$ N retention/absorbed nitrogen) were 63.3%, 58.0% and 61.6% for CSM, CAS, and CPL groups, respectively (p < 0.05). There was no difference in mean energy digestibility among treatments. The efficiency of absorbed lysine utilization was significantly different among treatments (p < 0.05). Pigs fed the CAS diet were inferior to counterparts on the other diets in utilizing absorbed lysine. The ratios of free (and small peptide-bound) to protein-bound amino acids in CSM diet differed considerably from the CAS diet. This may affect the efficiency of amino acids utilization for nitrogen retention if hydrolyzed and intact amino acid pools reach the blood at different times.

Quantitative Assay for the Binding of Jun-Fos Dimer and Activator Protein-1 Site

  • Lee, Sang-Kyou;Park, Se-Yeon;Jun, Gyo;Hahm, Eun-Ryeong;Lee, Dug-Keun;Yang, Chul-Hak
    • BMB Reports
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    • v.32 no.6
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    • pp.594-598
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    • 1999
  • The Jun and Fos families of eukaryotic transcription factors form heterodimers capable of binding to their cognate DNA enhancer elements. We are interested in searching for inhibitors or antagonists of the binding of the Jun-Fos heterodimer to the activator protein-1 (AP-1) site. The basic-region leucine zipper (bZIP) domain of c-Fos was expressed as a fusion protein with glutathione S-transferase, and allowed to form a heterodimer with the bZIP domain of c-Jun. The heterodimer was bound to glutathione-agarose, to which were added radiolabeled AP-1 nucleotides. After thorough washing, the gel-bound radioactivity was counted. The assay is faster than the coventional electrophoretic mobility shift assay because the gel electrophoresis step and the autoradiography step are eliminated. Moreover, the assay is very sensitive, allowing the detection of picomolar quantities of nucleotides, and is not affected by up to 50% dimethylsulfoxide, a solvent for hydrophobic inhibitors. Curcumin and dihydroguaiaretic acid, recently known inhibitors of Jun-Fos-DNA complex formation, were applied to this Jun-GST-fused Fos system and revealed to decrease the dimer-DNA binding.

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A Simple $N^{th}$ Best-Relay Selection Criterion for Opportunistic Two-Way Relay Networks under Outdated Channel State Information

  • Ou, Jinglan;Wu, Haowei;Wang, Qi;Zou, Yutao
    • KSII Transactions on Internet and Information Systems (TIIS)
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    • v.8 no.10
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    • pp.3409-3422
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    • 2014
  • The frequency spectrum available for the wireless communication is extremely crowded. In order to improve the spectral efficiency, the two-way relay networks have aroused great attention. A simple $N^{th}$ best-relay selection criterion for the opportunistic two-way relay networks is proposed, which can be implemented easily by extending the distributed timer technique in practice, since the proposed criterion is mainly based on the channel gains. The outage performance of the proposed relay selection scheme is analyzed under the outdated channel state information (CSI), and a tight closed-form lower bound and asymptotic value of the outage probability over Rayleigh fading channels are obtained. Simulation results demonstrate that the tight closed-form lower bound of the outage probability very closely matches with simulated ones in the whole SNR region, and the asymptotic results provide good tight approximations to the simulation ones, especially in the high SNR region.

Stochastic Upper Bound for the Stationary Queue Lengths of GPS Servers

  • Kim, Sung-Gon
    • The Korean Journal of Applied Statistics
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    • v.22 no.3
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    • pp.541-551
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    • 2009
  • Generalized processor sharing(GPS) service policy is a scheduling algorithm to allocate the bandwidth of a queueing system with multi-class input traffic. In a queueing system with single-class traffic, the stationary queue length becomes larger stochastically when the bandwidth (i.e. the service rate) of the system decreases. For a given GPS server, we consider the similar problem to this. We define the monotonicity for the head of the line processor sharing(HLPS) servers in which the units in the heads of the queues are served simultaneously and the bandwidth allocated to each queue are determined by the numbers of units in the queues. GPS is a type of monotonic HLPS. We obtain the HLPS server whose queue length of a class stochastically bounds upper that of corresponding class in the given monotonic HLPS server for all classes. The queue lengths process of all classes in the obtained HLPS server has the stationary distribution of product form. When the given monotonic HLPS server is GPS server, we obtain the explicit form of the stationary queue lengths distribution of the bounding HLPS server. Numerical result shows how tight the stochastic bound is.

Mg Delta-Doping Effect on a Deep Hole Center Related to Electrical Activation of a p-Type GaN Thin Film

  • Park, Hyo-Yeol;Jeon, Kyoung-Nam;Kim, Keun-Joo
    • Transactions on Electrical and Electronic Materials
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    • v.11 no.1
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    • pp.37-41
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    • 2010
  • The authors investigated the photoluminescence (PL) and the electron paramagnetic resonance (EPR) from an magnesium (Mg)-doped GaN thin film with a delta-doped layer. The regularly doped sample shows a PL peak at 2.776 eV for the as-grown sample, and the peak shifts to 2.904 eV and increases in intensity for the annealed sample. The delta-doped sample also shows the same PL peak as does the regularly doped sample. However, only the annealed delta-doped layer shows a sharp EPR with a small isotropic Lande g-factor, $g_{II}$, of 2.029. This resonance is attributed to the delta-doped layer, which forms a hole-bound Mg-N atomic structure instead of the $Mg_{Ga}-V_N$ defect complex, indicating that the delta-doped sample was not optically activated to form PL centers but was instead electrically activated to form a hole-bound state.

The cloning and characterization of the small GTP-binding Protein RacB in rice.

  • Jung, Young-Ho;Jaw, Nam-Soo
    • Proceedings of the Korean Society of Plant Pathology Conference
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    • 2003.10a
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    • pp.81.2-82
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    • 2003
  • Plants have evolved along with pathogens, and they have developed sophisticated defense systems against specific microorganisms to survive. G-protons are considered one of the upstream signaling components working as a key for the defense signal transduction pathway. For activation and inactivation of G-protein, GTP-biding proteins are involved. GTP -binding proteins are found in all organisms. Small GTP-binding proteins, having masses of 21 to 30kD, belong to a superfamily, often named the Ras supefamily because the founding members are encoded by human Ras genes initially discovered as cellular homologs of the viral ras oncogene. Members of this supefamily share several common structural features, including several guanine nucleotide binding domains and an effector binding domain. However, exhibiting a remarkable diversity in both structure and function. They are important molecular switches that cycle between the GDP-bound inactive form into the GTP-bound active form through GDP/GTP replacement. In addition, most GTP-binding proteins cycle between membrane-bound and cytosolic forms. such as the RAC family are cytosolic signal transduction proteins that often are involved in processing of extracellular stimuli. Plant RAC proteins are implicated in regulation of plant cell architecture secondary wall formation, meristem signaling, and defense against pathogens. But their molecular mechanisms and functions are not well known. We isolated a RacB homolog from rice to study its role of defense against pathogens. We introduced the constitutively active and the dominant negative forms of the GTP-hinging protein OsRacB into the wild type rice. The dominant negative foms are using two forms (full-sequence and specific RNA interference with RacB). Employing southern, and protein analysis, we examine to different things between the wild type and the transformed plant. And analyzing biolistic bombardment of onion epidermal cell with GFP-RacB fusion protein revealed association with the nucle.

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Anion-Dependent Exocyclic Mercury(II) Coordination Polymers of Bis-dithiamacrocycle

  • Siewe, Arlette Deukam;Kim, Seulgi;Choi, Kyu Seong;Lee, Shim Sung
    • Bulletin of the Korean Chemical Society
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    • v.35 no.12
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    • pp.3459-3464
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    • 2014
  • Synthesis and structural characterization of mercury(II) halides and perchlorate complexes (1-4) of bis-$OS_2$-macrocycle (L) are reported. L reacts with mercury(II) chloride and bromide to yield an isostructural 2D coordination polymers with type $[Hg(L)X_2]_n$ (1: X = Cl and 2: X = Br). In 1, each Hg atom which lies outside the cavity is six-coordinate with a distorted octahedral geometry, being bound to four adjacent ligands via monodentate Hg-S bonds and two remaining sites are occupied by two terminal chlorido ligands to form a fishnet-like 2D structure. When reacting with mercury(II) iodide, L afforded a 1D coordination polymer $\{[Hg_2(L)I_4]{\cdot}CHCl_3\}_n$ (3) in which each exocyclic Hg atom is four-coordinate, being bound to two sulfur donors from different ligands doubly bridging the ligand molecules in a head-to-tail mode. The coordination sphere in 3 is completed by two iodo terminal ligands, adopting a distorted tetrahedral geometry. On reacting with mercury(II) perchlorate, L forms solvent-coordinated 1D coordination polymer $\{[Hg_2(L)(DMF)_6](ClO_4)_4{\cdot}2DMF\}_n$ (4) instead of the anion-coordination. In 4, the Hg atom is five-coordinate, being bound to two sulfur donors from two different ligands doubly bridging the ligand molecules in a side-by-side mode to form a ribbon-like 1D structure. The three remaining coordination sites in 4 are completed by three DMF molecules in a monodentate manner. Consequently, the different structures and connectivity patterns for the observed exocyclic coordination polymers depending on the anions used are influenced not only by the coordination ability of the anions but also by anion sizes.

Achievable Sum Rate Analysis of ZF Receivers in 3D MIMO Systems

  • Li, Xingwang;Li, Lihua;Xie, Ling
    • KSII Transactions on Internet and Information Systems (TIIS)
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    • v.8 no.4
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    • pp.1368-1389
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    • 2014
  • Three-dimensional multiple-input multiple-output (3D MIMO) and large-scale MIMO are two promising technologies for upcoming high data rate wireless communications, since the inter-user interference can be reduced by exploiting antenna vertical gain and degree of freedom, respectively. In this paper, we derive the achievable sum rate of 3D MIMO systems employing zero-forcing (ZF) receivers, accounting for log-normal shadowing fading, path-loss and antenna gain. In particular, we consider the prevalent log-normal model and propose a novel closed-form lower bound on the achievable sum rate exploiting elevation features. Using the lower bound as a starting point, we pursue the "large-system" analysis and derive a closed-form expression when the number of antennas grows large for fixed average transmit power and fixed total transmit power schemes. We further model a high-building with several floors. Due to the floor height, different floors correspond to different elevation angles. Therefore, the asymptotic achievable sum rate performances for each floor and the whole building considering the elevation features are analyzed and the effects of tilt angle and user distribution for both horizontal and vertical dimensions are discussed. Finally, the relationship between the achievable sum rate and the number of users is investigated and the optimal number of users to maximize the sum rate performance is determined.