• Journal of Nutrition and Health
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• v.29 no.6
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• pp.590-593
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• 1996

This study was done to evaluate the effect of dietary calcium level (a diet which met 100% or twice the calcium level in AIN-76 diet) on preventing bone loss in ovariectomized rats. Forty Sprauge-Dawley female rats(body weight 200$\pm$5g)were divided into two groups. One group were ovariecotomized (Ovx) while the others received sham operation(Sham). Thereafter, each rat group was further divided into normal calcium diet(0.52%) and high calcium diet(1.04%) subgroups. All rats were fed on experimental diet and deionized water ad libitum for 8 weeks. The total body, spine and femur bone mineral densities and bone mineral contents were measured by Dual Energy X-ray Absorptiometry, Eight weeks following operation, ovariectomized rats fed a high calcium diet had a significantly higher total bone mineral content, total bone calcium content, spine bone mineral density, spine bone mineral content and femur bone mineral content than ovariectomized rats fed control calcium diet. The correlation between dietary calcium intake level and spine bone mineral density were positive, but there was no correlation between dietary calcium intake and femur bone mineral density. The findings from the present study demonstrated that bone loss due to ovarian hormonal deficiency can be partially prevented by a high calcium diet. Futhermore, these findings support the strategy of the use of a high calcium diet in the prevention of estrogen depleted bone loss(postmenopausal osteoporosis)

• Journal of Nutrition and Health
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• v.30 no.3
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• pp.266-276
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• 1997

To investigate the effects of dietary protein and calcium levels on calcium and bone metabolism Sprague-Dawley male growing rats weighting approximately 91.4g were divided into four groups and fed one of the following four experimental diets-15% protein 0.2% calcium ; 15% protein 0.5% calcium ; 30% protein 0.2% calcium ; 30% protein 0.5% calcium-for five weeks. Calcium intake and excretion, apparent calcium absorption were measured and bone densities and mineral contents of femur and scapula were analyzed. Calcium excretion through feces and urine was significantly greater in animals receiving diets of higher calcium. Fecal calcium but not urinary calcium excretion was greater when the protein level was increased from 15% to 30%. Apparent calcium absorption rate was significantly higher with lower calcium intakes. Serum alkaline phosphatase activity was significantly higher in 0.2% calcium group than in 0.5% calcium group, while urinary hydroxyproline excretion was essentially same among all experimental groups. Weights and mineral contents or protein. Bone weights were greater, but calcium and ash contents of femur and scapula were lower in animals on the diet containing low calcium and high protein, which suggests that bone metabolism may be affected by the interaction between calcium and protein intake. These results indicate that during growth high protein intake might be beneficial to bone health if the diet is sufficient in calcium, however, if the diet fails to provide an optimum amount of calcium, such practice might be detrimental.

• Nutrition Research and Practice
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• v.1 no.1
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• pp.70-73
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• 2007

This study was conducted to determine the nutritional characteristics of horsemeat and bone meal in comparison with those of beef and pork presented by Dietary Reference Intakes For Koreans. Longissimus muscle and large metacarpal bone samples were collected from 20 fattened Jeju horses. Muscle samples were subjected to proximate analysis, assays for fatty acid profile and minerals, and bone samples to mineral assays. Horsemeal had similar levels of protein (21.1 vs 21.0 or 21.1%) and lower levels of fat (6.0 vs 14.1 or 16.1%) compared with beef or pork, respectively. Horsemeat had much higher levels of palmitoleic (8.2 vs 4.4 or 3.3%) and $\alpha-linolenic$ (1.4 vs 0.1 or 0.6%) acids than beef or pork, respectively. Linoleic acid was much higher in horsemeat (11.1%) and pork (10.1%) than in beef (1.6%). PUFA:SFA and n-6:n-3 ratios in horsemeat were 0.29 and 10.2, respectively. There were no big differences in mineral contents between horsemeat, beef and pork. For daily recommended mineral intakes of male adults (Dietary Reference Intakes For Koreans), phosphorus, sodium, potassium, iron, zinc and copper can be provided up to 24, 2.5, 6.7, 21, 26 and 40%, respectively, by 100 g raw horsemeat, but calcium and manganese levels are negligible. Horse cannon bone had much higher mineral contents especially in calcium (10,193 mg/100 g), phosphorus (5,874 mg/100 g) and copper (0.79 mg/100 g). Thiamin, riboflavin, niacin and retinol contents were 0.20, 0.21, 1.65 mg/100 g and $30{\mu}g/100g$, respectively. But ascorbic acid and beta-carotene were not detected. Our data demonstrated that higher levels of palmitoleic and $\alpha-linolenic$ acid in horsemeat than in beef and pork may be beneficial for human health. Horsemeat and bone meal are a good source of some minerals and vitamins.

• Proceedings of the Korea Contents Association Conference
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• 2009.05a
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• pp.1141-1149
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• 2009

In Bone Mineral Density(BMD) measurements, accuracy and precision must be superior in order to know the small changes in bone mineral density and actual biological changes. Therefore the purpose of this study is to increase the reliability of bone mineral density inspection through appropriate management of image quality from machines and inspectors. For the machine management method, the recommended phantom from each bone mineral density machine manufacturer was used to take 10~25 measurements to determine the standard amount and permitted limit. On each inspection day, measurements were taken everyday or at least three times per week to verify the whether or not change existed in the amount of actual bone mineral density. Also evaluations following Shewhart control chart and CUSUM control chart rules were made for the bone mineral density figures from the phantoms used for measurements. Various forms of management became necessary for machine installation and movement. For the management methods of inspectors, evaluation of the measurement precision was conducted by testing the reproducibility of the exact same figures without any real biological changes occurring during reinspection. There were two measurement methods followed: patients were either measured twice with 30 measurements or three times with 15 measurements. An important point to make regarding measurements is that after the first inspection and any other inspection following, the patient was required to come off the inspection table completely and then get back on for any further measurements. With a 95% confidence level, the precision error produced from the measurement bone mineral figures produced a precision error of 2.77 times the minimum of the biological bone mineral density change (Least significant change: LSC). In order to assure reliability in inspection, there needs to be good oversight of machine management and measurer for machine operation and inspection error. Accuracy error in machines needs to be reduced to under 1% for scientific development in bone mineral density machines.

• Preventive Nutrition and Food Science
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• v.8 no.3
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• pp.245-248
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• 2003

This study was undertaken to investigate the effects of the Ginseng radix in osteoporosis of the oophorectomized rats. In this experiment, the rats were oophorectomized and administered the water extracts of the Ginseng radix. The spinal bone mineral density, calcium, phosphorus and ash weight of the bones were measured. The spinal bone mineral density was significantly increased in the ovariectomized (OVX)-Ginseng radix group at 8 weeks as compared to the OVX-saline group. Furthermore, the calcium and phosphorus contents of the femoral and fibula-tibia were significantly increased in the OVX-Ginseng radix group as compared to the OVX-saline group. The ash weights of the femoral and fibula-tibial bones were increases in the OVX-Ginseng Radix group, although it was not statistically significant. On reviewing these experiments, it appears that the Ginseng radix possess efficacy for the prevention of osteoporosis. Further study would be of value to confirm the efficacy of the Ginseng radix for the treatment and/or prevention of osteoporosis in humans.

• Journal of Nutrition and Health
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• v.38 no.7
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• pp.544-552
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• 2005

This study was conducted to examine the correlation among bone mineral density (BMD), hair and nail calcium and magnesium contents, and nutrient intakes in preschool children in Chungnam district. A total of 111 preschool children (61 boys, 50 girls) measured the anthropometric characteristics, the bone mineral density of carpus using DEXA and hair and nail calcium and magnesium contents were analyzed by ICP spectrometer. The average ages of the study subjects were 55.7 months in boys and 53.0 months in girls (3.8${\~}$7 years). The birth height and weight of subjects were 51.0 cm and 3.3 kg in boys and 50.3cm and 3.2kg in girls, respectively. The average height, weight, $\%$ body fat, and obesity index were 111.6 cm, 19.6 kg, $15.0\%,\;96.1\%$ in boys and 108.6 cm, 18.5 kg, $17.5\%,\;98.1\%$ in girls, respectively. The bone mineral density in carpus was $0.25 g/cm^2$ in boys and $0.24 g/cm^2$ in girls. The hair calcium and magnesium contents were 440.9 ppm, 16.4 ppm in boys and 373.2 ppm, 28.9 ppm in girls. The nail calcium and magnesium contents were 9.4 ppm, 2.1 ppm in boys and 10.4 ppm, 2.4 ppm in girls. The intakes of energy, calcium, iron, zinc, vitamin A. niacin, and vitamin C did not meet the Korean RDAs. The ultradis and average carpus BMD were positively correlated to the plant calcium intake (p < 0.05, p < 0.05). And the ultradis, distal, and average carpus BMD were positively correlated to the plant iron intake (p < 0.05, p < 0.05, p < 0.05). The average bone mineral density in the carpus was positively correlated to the nail magnesium content (p < 0.05). Therefore, more systematic studies to investigate the roles of iron and calcium intakes, nail magnesium content in bone development of preschool children were required.

• Journal of the East Asian Society of Dietary Life
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• v.12 no.3
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• pp.218-224
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• 2002

This study was undertaken to analyze chemical composition, macro- and micro-mineral contents of ostrich bone with flesh meat (BF), ostrich bone with flesh meat with herb medicine (BFll) and ostrich bone with internal organs (BI). Crude fat of the BFH was lower, but crude protein and crude ash of the BFH were significantly higher than those of the BF and the BI. Among the minerals, Ca, p, Mg, Fe, Cu, Zn content of the BFH were significantly higher than the content in the BF and the BI. The minerals, Pb, Cd and As were not detected in ostrich and herb medicine extracts. From the sensory test of ostrich and herb medicine, the preferences were in the fellowing order; ostrich bone with flesh meat with herb medicine (BFH), ostrich bone with flesh meat (BF), and ostrich bone with internal organs (BI) in extracts.

• Korean journal of food and cookery science
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• v.6 no.4 s.13
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• pp.21-26
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• 1990

This study was undertaken to determine the content of calcium, phosphorus, and magnesium in Korean styled Sagol bone stok (beef leg bone stock) deffering in cooking utensil, cooking method, and cooking time. Cooking was started in cold water and boiling water using stainless steel and aluminium cooker. In the changes of mineral contests in Sagol bone stock at cooking time from 2 to 12 hours, Ca, P, and Mg contents increased significantly by the cooking time in stainless steel and aluminium cooker but Ca and P contents decreased slightly during 12 hours in steel cooker. But Ca, P, and Mg contents of the Sagol bone stock were not significantly different between cooking utensil and method. The extracting rate of Ca, P, and Mg of the Sagol bone stock were $0.02{\sim}0.05%$, $0.2{\sim}0.7%$, and $0.1{\sim}1.0%$, respectively. The extracting rate of Ca was positively correlated with that of Mg (r=0.8872, p<0.001).

• Journal of the East Asian Society of Dietary Life
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• v.22 no.4
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• pp.480-488
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• 2012

Polyphenols may be primarily responsible for the health benefits associated with tea consumption. However, the benefits of the tea polyphenols to bone health have not been studied well. The purpose of this study was to investigate the effects of Oolong tea on bone mineral density and bone-related markers in ovariectomized rats. Sprague-Dawley female rats were randomly assigned to Sham-Control, Sham-Oolongtea, Ovx-Control, and Ovx-Oolongtea groups. Bone mineral density (BMD) and bone mineral content (BMC) were estimated by using PIXImus (GE Lunar Co, Wisconsin.) in the spine and femur. After 6 weeks of the experimental diets, body weight gain, food intake, and food efficiency ratio (FER) were significantly higher in Ovx groups than in Sham groups regardless of diet. The serum concentration of calcium, phosphorus, ALP, and calcitonin were not significantly different according to Oolong tea supplementation. There were no significant differences in urinary calcium and phosphorus excretion between all groups. The urinary DPD crosslinks value was significantly higher in the Ovx-Control group than in the Sham-Oolongtea group. Spine BMD, femur BMD, and spine BMD per body weight were significantly lower in the Ovx groups than in the Sham groups regardless of diet. In the OVX group, spine BMC per body weight, femur BMD per body weight and femur BMC per body weight were significantly higher in the Oolong tea groups than in Control groups. It was concluded that Oolong tea supplementation positively influenced bone health in ovariectomized rats.

• The Journal of the Korea Contents Association
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• v.9 no.5
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• pp.214-223
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• 2009

In this study we investigated the impact of exercise program through the grip strength, the balance and the bone mineral density of elderly women in rural community. A total of number 56 elderly women were divided into two groups, the experimental group(n=33) and the control group(n=23). The experimental group participated exercise program during 13 weeks from 3 Oct to 29 Decem in 2005, twice a week 60 minutes per session. Among the experimental group, 33 people who participated over 20 times exercise program were chosen as the subjects of analysis. The results of this study were as follows: The grip strength was significantly increased in the experimental group compared with the control group(p<0.05). The Timed up and go test and Bone Mineral Density were significantly increased in the experimental group compared with the control group(p<0.01). In conclusion, there has been a substantial improvement in 13 weeks by exercise program is recommended to elderly women in aspect of increasing the grip strength, the balance and the bone mineral density.