Analysis of Mineral Contents in Tissues and Bone Mineral Density of Rats

흰쥐의 조직 중 무기질 함량과 골밀도 분석

  • Kim, Myung-Hee (Dept. of Food and Nutrition, Kongju National University) ;
  • Kim, Moo-Kyung (Dept. of Food and Nutrition, Kongju National University) ;
  • Lee, Jong-Wan (Dept. of Animal Resources Science, Kongju National University) ;
  • Kim, Mi-Hyun (Dept. of Food and Nutrition, Kangwon National University) ;
  • Kang, Myung-Hwa (Dept. of Food Science and Nutrition, Hoseo University) ;
  • Choi, Mi-Kyeong (Dept. of Food and Nutrition, Kongju National University)
  • 김명희 (공주대학교 식품영양학과) ;
  • 김무경 (공주대학교 식품영양학과) ;
  • 이종완 (공주대학교 동물자원학과) ;
  • 김미현 (강원대학교 식품영양학과) ;
  • 강명화 (호서대학교 식품영양학과) ;
  • 최미경 (공주대학교 식품영양학과)
  • Received : 2005.05.20
  • Accepted : 2010.08.20
  • Published : 2010.09.30

Abstract

The purpose of present study was to analyze mineral contents in various tissues and investigate theirs relation with bone mineral density (BMD) in rats. Fifteen Sprague-Dawley rats were fed standard diet for 4 weeks. Body weight gain, feed intake, and feed efficiency ratio were 41.00 g/week, 171.15 g/week, and 0.24 respectively. Among 12 minerals in serum, Ca is the highest with 6.86 mg/dl. Serum Mg, Se, and Cu were 2.52 mg/dl, 0.23 mg/dl and 0.22 mg/dl respectively. Mg contents in liver, spleen, and kidney were $246.36\;{\mu}/g$, $105.01\;{\mu}/g$, and $273.38\;{\mu}/g$ respectively. Tibia contents of Ca, Mg, Zn, Fe and V were 194.91 mg/g, 23.10 mg/g, 0.60 mg/g, 0.35 mg/g and 0.14 mg/g respectively. BMDs of right tibia and spine were 122.04 mg/cm and $153.61\;mg/cm^2$. There were significantly positive correlations between tibia BMD and Se (p<0.05), tibia BMD and V (p<0.01), spinal BMD and V(p<0.05), respectively. It's expected that these results are used as a reference data in following study to elucidate physiological function of minerals.

Keywords

References

  1. Barbagallo M, Belvedere M, Dominguez LJ (2009): Magnesium homeostasis and aging. Magnes Res 22:235-246.
  2. Bell GH (1952): Bone as a skeletal structure. Br J Nutr 6:405-409. https://doi.org/10.1079/BJN19520045
  3. Brown RW (1956): Magnesium; its role in metabolism. J Kans Med Soc 57:170-179.
  4. Cortizo AM Etcheverry SB (1995): Vanadium derivatives act as growth factor-mimetic compounds upondifferentiation and proliferation of osteoblast-like UMR-106 cells. Mol Cell Biochem 145:97-102.
  5. Cortizo AM, Brussone L, Molinuevo S, Etcheverry SB (2000): Vanadate-induced nitric oxide production: role in osteoblast growth and differentiation. Eur J Pahrmacol 400:279-285. https://doi.org/10.1016/S0014-2999(00)00356-3
  6. Cotzias GC (1964): Trace metals: essential or detrimental to life. Tech Rep Brookhaven Natl Lab 10:1-14.
  7. Facchini DM, Yuen VG, Battell ML, McNeill JH, Grynpas MD (2006): The effects of vanadium treatment on bone in diabetic and non-diabetic rats. Bone 38:368-377. https://doi.org/10.1016/j.bone.2005.08.015
  8. Ishimi Y (2010): Nutrition and bone health. Magnesium and bone. Clin Calcium 20:762-767.
  9. Jun YS, Choi MK, Kim AJ, Kim MH, Sung CJ (2002): Effect of iron supplementation on mineral utilization in rats. J Korean Soc Food Sci Nutr 31:658-663. https://doi.org/10.3746/jkfn.2002.31.4.658
  10. Kim MH, Kim YR, Lee JW, Park BK, Kim MK, Choi MK, Kim AJ (2008): The effects of caffeine on lipid and mineral content in the serum of rats. Korean J Food & Nutr 21:336-343.
  11. Nelson TS, Hargus WA, Storer N, Walker AC (1965): The influence of calcium on phosphorus utilization by chicks. Poult Sci 44:1508-1513. https://doi.org/10.3382/ps.0441508
  12. Nordin BE (1964): The blood-bone equilibrium. Sci Basis Med Annu Rev 308-316.
  13. Owen EC (1952): Bone as a mineral reserve. Br J Nutr 6:415-423. https://doi.org/10.1079/BJN19520047
  14. Reeves PG (1997): Components of the AIN-93 diets as improvements in the AIN-76A diet. J Nutr 127:838S-841S.
  15. Underwood EJ (1976): Trace Elements in Human and Animal Nutrition. 4th ed. Academic Press, New York, pp 2-15.
  16. Widdowson EM (1974): Trace elements in fetal and early postnatal development. Proc Nutr Soc 33:275-284. https://doi.org/10.1079/PNS19740050
  17. Zhang SQ Chen GH, Lu WL, Zhang Q (2007): Effects on the bones of vanadyl acetylacetonate by oral administration: a comparison study in diabetic rats. J Bone Miner Metab 25:293-301. https://doi.org/10.1007/s00774-007-0759-7