• 대한의용생체공학회:의공학회지
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• 제39권1호
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• pp.22-29
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• 2018

Intervertebral disc(IVD) mainly consists of Annulus fibrosus(AF) and Nucleus pulposus(NP), playing a role of distributing a mechanical load on vertebral body. IVD tissue engineering has been developed the methods to achieve anatomic morphology and restoration of biological function. The goal of present study is to identify the possibilities for creating a substitute of IVD the morphology and biological functions are the same as undamaged complete IVD. To fabricate the AF and NP combine biphasic IVD tissue, AF tissue scaffolds have been printed by 3D bio-printing system with natural biomaterials and NP tissues have been prepared by scaffold-free culture system. We evaluated whether the combined structure of 3D printed AF scaffold and scaffold-free NP tissue construct could support the architecture and cell functions as IVD tissue. 3D printed AF scaffolds were printed with 60 degree angle stripe patterned lamella structure(the inner-diameter is 5mm, outer-diameter is 10 mm and height is 3 mm). In the cytotoxicity test, the 3D printed AF scaffold showed good cell compatibility. The results of histological and immunohistochemical staining also showed the newly synthesized collagens and glycosaminoglycans, which are specific makers of AF tissue. And scaffold-free NP tissue actively synthesized glycosaminoglycans and type 2 collagen, which are the major components of NP tissue. When we combined two engineered tissues to realize the IVD, combined biphasic tissues showed a good integration between the two tissues. In conclusion, this study describes the fabrication of Engineered biphasic IVD tissue by using enable techniques of tissue engineering. This fabricated biphasic tissue would be used as a model system for the study of the native IVD tissue. In the future, it may have the potential to replace the damaged IVD in the future.

• 한국소음진동공학회:학술대회논문집
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• 한국소음진동공학회 2003년도 추계학술대회논문집
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• pp.572-578
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• 2003

The free vibration of a spinning flexible disk-spindle system supported by hydro dynamic bearings in a HDD is analyzed by FEM. The spinning flexible disk is described using Kirchhoff plate theory and von Karman non-linear strain, and its rigid body motion is also considered. It is discretized by annular sector element. The rotating spindle which includes the clamp, hub, permanent magnet and yoke, is modeled by Timoshenko beam including the gyroscopic effect. The flexible supporting structure with a complex shape which includes stator core, housing, base plate, sleeve and thrust pad is modeled by using a 4-node tetrahedron element with rotational degrees of freedom to satisfy the geometric compatibility. The dynamic coefficients of HDB are calculated from the HDB analysis program, which solves the perturbed Raynolds equation using FEM. Introducing the virtual nodes and the rigid link constraints defined in the center of HDB, beam elements of the shaft are connected to the solid elements of the sleeve and thrust pad through the spring and damper element. The global matrix equation obtained by assembling the finite element equations of each substructure is transformed to the state-space matrix-vector equation, and the associated eigenvalue problem is solved by using the restarted Arnoldi iteration method. The validity of this research is verified by comparing the numerical results of the natural frequencies with the experimental ones. Also the effect of supporting structures to the natural modes of the total HDD system is rigorously analyzed.

• Journal of Biosystems Engineering
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• 제39권1호
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• pp.21-33
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• 2014

Background: Significant technological development and changes happened in the tractor industries. Contrariwise, the test procedures of the major standard development organizations (SDO's) remained unchanged or with a little modification over the years, demanding new tractor test standards or improvement of existing ones for tractor performance, safety, and comfort. Purpose: This study focuses on reviewing the research trends regarding performance, safety and comfort evaluation of agricultural tractors. Based on this review, few recommendations were proposed to revise or improve the current test standards. Review: Tractor power take-off power test using the DC electric dynamometer reduced human error in the testing process and increased the accuracy of the test results. GPS signals were used to determine acceleration and converted into torque. High capacity double extended octagonal ring dynamometer has been designed to measure drawbar forces. Numerical optimization methodology has been used to design three-point hitch. Numerous technologies, driving strategies, and transmission characteristics are being considered for reducing emissions of gaseous and particulate pollutants. Engine emission control technology standards need to be revised to meet the exhaust regulations for agricultural tractors. Finite Element Analysis (FEA) program has been used to design Roll-Over Protective Structures (ROPS). Program and methodology has been presented for testing tractor brake systems. Whole-body vibration emission levels have been found to be very dependent upon the nature of field operation performed, and the test track techniques required development/adaptation to improve their suitability during standardized assessment. Emphasizes should be given to improve visibility and thermal environment inside the cab for tractor operator. Tractors need to be evaluated under electromagnetic compatibility test conditions due to large growing of electronic devices. Research trends reviewed in this paper can be considered for possible revision or improvement of tractor performance, safety, and comfort test standards.

• Macromolecular Research
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• 제16권8호
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• pp.695-703
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• 2008

Nanoparticles have tremendous potential in cancer prevention, detection and augmenting existing treatments. They can target tumors, carry imaging capability to document the presence of tumors, sense pathophysiological defects in tumor cells, deliver therapeutic genes or drugs based on the tumor characteristics, respond to external triggers to release an appropriate agent, document the tumor response, and identify the residual tumor cells. Nanoparticles < 30 nanometers in diameter show unexpected and unique properties. Furthermore, particles < 5 nanometers in size can easily penetrate cells as well as living tissues and organs. This study evaluated the safety of nano materials in a living body and the relationship between the living tissue and synthetic nano materials by examining the in-vitro cytotoxicity of poly(lactic-co-glycolic) acid (PLGA) nano-spheres and fluorescein isothiocynate(FITC)-labeled dendrimers as polymer nanoparticles. PLGA was chosen because it has been used extensively for biodegradable nanoparticles on account of its outstanding bio-compatibility and its acceptance as an FDA approved material. The dendrimer was chosen because it can carry a molecule that recognizes cancer cells, a therapeutic agent that can kill those cells, and a molecule that recognizes the signals of cell death. Cytotoxicity in L929 mouse fibroblasts was monitored using MTT assay. Microscopic observations were also carried out to observe cell growth. All assays yielded meaningful results and the PLGA nanoparticles showed less cytotoxicity than the dendrimer. These nano-particles ranged in size from 10 to 100 nm according to microscopy and spectroscopic methods.

• 대한화학회지
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• 제38권7호
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• pp.473-479
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• 1994

저압 화학 기상 증착법을 사용하여 $WF_6$의 환원반응으로 텅스텐 박막을 p형 실리콘 (100)표면위에 증착하였다. Cold-wall조건에서는 실리콘 기판과 $SiH_4$를 각각 이용하여 $WF_6$를 환원시켜 텅스텐 박막을 증착하였으며 hot-wall 조건에서는 $WF_6$를 $SiH_4$로 환원시켜 증착하였다. 박막의 결정구조는 어느 조건에서나 체심입방구조를 이루었으며, 증착조건에 따른 박막의 물리적 및 전기적 특성을 조사하였다. 증착된 박막을 온도 $800^{\circ}C$에서 열처리한 결과 hot-wall 조건의 박막이 $WSi_2$로 변화하였다. Hot-wall과 cold-wall조건에서의 박막을 분석한 결과 박막의 특성은 cold-wall조건이 우수하나 hot-wall조건에서는 열처리 방법에 의하여 실리콘 기판과 적합성이 우수한 것으로 알려진 $WSi_2$ 박막의 제조가 가능함을 알 수 있었다.

• 한국항해항만학회지
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• 제28권8호
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• pp.715-719
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• 2004

본 논문은 개인용 컴퓨터(PC)에 적합한 EMC 필터의 제작에 대해서 다룬다. PC는 CPU를 포함한 여러 소자들이 모여 있어 각 장치 간에 서로 많은 간섭이 일어날 수 있고, PC와 연결되는 여러 외부 장치들에 영향을 받아 불요 전자파가 많이 발생필 수 있다. 따라서 본 연구에서는 PC를 측정대상기기(EUT)로 하여 임피던스에 적합한 노이즈 저감 필터를 제작하였다. 관통형(Feed-through) 컨덴서와 높은 투자율을 가진 페라이트 비드로 구성된 필터를 제작한 필터와 함께 적용하여 PC용 EMC 필터를 제작하였다. 실험을 통해서 제작한 필터가 10 MHz～1.5 GHz에서 삽입손실이 40 dB 이상 감쇠되며, CISPR Pub. 22 규격과 IEC 61000-4-4의 level 4까지 만족함을 알 수 있었다.

• 한국전자파학회논문지
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• 제23권8호
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• pp.942-949
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• 2012

대규모 공공시설의 안전성을 확보하기 위해서는 시설에 포함되는 전자 시스템의 전자파 적합성에 대한 분석은 매우 필요하다. 이는 시스템을 구성하는 개별 기기가 기존의 전자파 적합성 규격을 만족하더라도 개별 기기의 배치와 상호 접속에 따라 시스템-레벨에서의 전자파 적합성은 보장되지 않기 때문이다. 따라서 안전 및 임무에 임계적인 전자 시스템의 안전을 확립하기 위해서는 시스템-레벨의 전자파 적합성에 대한 평가 및 분석은 반드시 이루어져야 한다. 본 논문에서는 국내, 외의 관련 제도를 분석하고, 나아가서 시스템-레벨에서의 전자파 적합성 접근 방법과 전기철도, 빌딩, 자동차, 정유공장에서의 구체적 적용 사례를 조사, 분석하였다. 또한, 관련 제도 도입 방안을 제안하고, 제안된 각 방안에서 주요하게 다루어야 할 부분과 문제점에 대해 살펴보았다. 이러한 연구 결과가 전자파 엔지니어링 제도 도입에 활용되어 국가 보안 및 국민의 생명 신체 및 재산을 보호하는데 기여할 수 있을 것으로 기대한다.

• 한국의류학회지
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• 제26권7호
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• pp.1093-1104
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• 2002

In this study, the diffusion phenomenon of casual Hanbok which started in the mid 1990s and has been widely spread ever since with innovative features in design, materials, etc., was reviewed. Through the interviews of 45 People who have worn casual Hanbok the advantages and disadvantages of casual Hanbok related to its innovative characteristics were recorded and analyzed. Through the questionnaires distributed to 482 adults living in Seoul, Busan, and Darjeon, the factors related to the adoption of casual Hanbok were examined. The following are the results from the empirical study. First the adoption of contemporary casual Hanbok is due to the consumers′ positive response to the excellence of casual Hanbok′s various innovative characteristics. What the consumers perceived as innovative characteristics of casual Hanbok in the survey results validated the findings of the depth interview, identifying seven subcategories of the innovative characteristics of casual Hanbok; aesthetics, positive training effect on body and mind, difficulty in we, practicality, psychological attachment for "Our Clothes", economic efficiency, and symbolic compatibility. And consumers who think highly of such spiritual and identity values as national consciousness, nature affiliation, and community consciousness, had high evaluation to casual Hanbok However, consumers′ prejudice against Hanbok that Hanbok is for old people, uncomfortable, and not suitable to modern life, tuned out to have a negative influence on the adoption. From these results, it can be concluded that casual Hanbok of the late 1990s is a fashion with innovative characteristics, different from the one in the l980s worn by the demonstrators of popular movements and democratic movements, and it emerged as a new symbol with the characteristics of cultural movement like community consciousness and nature affiliation.

• 공업화학
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• 제16권6호
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• pp.790-793
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• 2005

최근 인간의 생명 연장과 건강 등에 관심이 많아지면서 약학 및 의학계는 생체 내에서 보다 안정적이며 효과를 나타낼 수 있는 약물 전달 시스템의 개발에 많은 힘을 기울이고 있는 실정이다. 수많은 생화학 연구자들은 키토산이 인체에 거부반응이 없으며 약물과 백신의 전달을 효율적이고 안전하게 흡착능력을 향상시킨다는 것을 밝혀 왔다. 또한 그것은 생분해성, 생체 친화적이라는 장점 때문에 약물 방출 조절에 적당하다고 알려져 있다. 본 연구에서는 생명고분자인 키토산의 나노입자를 제조하여 농도, pH, 최적 온도에서 약물 전달 조절을 in vivo 조건에서 수행하였다. 인슐린을 담지한 키토산 나노입자는 당뇨성 쥐의 혈당을 효과적으로 낮춰 줄 수 있음을 알 수 있었다.

• 문화기술의 융합
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• 제8권4호
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• pp.415-422
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• 2022

이기종인 온주감귤 유전자(A=20.57, C=32.71, G=30.01, U=16.71%)와 커피 유전자(A=20.66, C=31.76, G=30.187, U=16.71%)는 95%이상이 유전자비가 동일하다. 이기종이면 일반적으로 유전자 결합이 안 되는 것으로 알려졌다. 그러나 유전자 기능적-유사성이 95%이상에서 Chargaff 룰과 Shannon Entropy 조건을 만족하면 접목이 가능하며, 새품종인 Coffrange가 된다. 우리는 DNA-RNA를 세계최초 BCJM 행렬로 풀어 미국특허 및 국제저널에 발표했다. 모든 동식물과 바이러스도 사람이 유전자와 비슷하다. 이점에 착안, 코로나-19와 인체의 유전자 특성을 풀어 영국 행렬교재에 6월 발표했다. 식물에서는 유전자 위치를 쉽게 바꾸는 기법인 BCJM-Transposon으로 처리한다. 시뮬레션에서는 행렬이 Cut & Paste와 Transpose로 성공할 수 있음을 예측했다.