• Journal of Periodontal and Implant Science
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• 제40권2호
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• pp.61-68
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• 2010

Purpose: The present study was performed to clarify the relationship between periodontal disease severity and selected immunological parameters consisting of serum IgG titer against periodontopathogenic bacteria, the expression of the helper T-cell cytokine by gingival mononuclear cells, and patients' immunoreactivity to cross-reactive heat shock protein (HSP) epitope peptide from P. gingivalis HSP60. Methods: Twenty-five patients with moderate periodontitis had their gingival connective tissue harvested of gingival mononuclear cells during an open flap debridement procedure and peripheral blood was drawn by venipuncture to collect serum. The mean level of interproximal alveolar bone was calculated to be used as an index for periodontal disease severity for a given patient. Each of selected immunologic parameters was subject to statistical management to seek their correlations with the severity of periodontal disease. Results: A significant correlation could not be identified between serum IgG titers against specific bacteria (Porphyromonas gingivalis, Prevotella intermedia, Fusobacterium nucleatum, Actinobacillus actinomycetemcomitans, and Streptococcus mutans) and the severity of periodontal disease. Expression of interleukin (IL)-10 by gingival mononuclear cells was statistically significant in the group of patients who had higher levels of alveolar bone height. However, a similar correlation could not be demonstrated in cases for IL-4 or interferon-$\gamma$. Patients' serum reactivity to cross-reactive epitope peptide showed a significant correlation with the amount of alveolar bone. Conclusions: It was concluded that expression of IL-10 by gingival mononuclear cells and patients' sero-reactivity to the cross-reactive HSP peptide of P. gingivalis HSP60 were significantly correlated with alveolar bone height.

• 대한임상검사과학회지
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• 제48권3호
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• pp.196-201
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• 2016

효과적인 비만의 중재는 대상 인구에서 가장 관련성이 있는 인구통계학적 건강 관련 및 직무 관련 요인을 고려해야 한다. 가장 강력한 비만 관련 요인은 직업군 또는 연령 범주에서 다룰 수 있다. 이 연구의 목적은 심리적인 직무 스트레스와 면역관련 인자의 혈액분석의 상관성에 대한 최신지견을 제공하는 것이다. 우리는 여성 근로자의 비만 관련 매개 변수 뿐만 아니라 자연살해세포활성(NKCA)을 평가하였다. 비만과 관련된 혈당(p<0.05)은 연령에서 유의한 차이를 보였다. 또한, 비만군과 non-비만군에서는 NK 세포의 활성에 큰 차이를 보였다(p<0.05). 단계적 다중 회귀 분석에서 NKCA는 비만 관련 요인과 밀접한 상관성을 보였다. 향후에는 면역인자들의 활성도가 증가하는 생물학적 변화를 연구해야 할 것이다.

• Animal Bioscience
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• 제34권3_spc호
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• pp.363-370
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• 2021

Objective: The effect of garlic powder (GP) and phenyl acetic (PA) acid throughout the fattening period of broiler chickens on performance, blood parameters, immune, and antioxidant parameters as well as carcass traits was evaluated. Methods: A total of 210 day-old Cobb broiler chicks were randomly distributed into seven dietary treatments having five replications with six chicks per replicate. The first group (control) fed a basal diet without supplements, whereas the 2nd, 3rd, and 4th group were fed basal diet plus 0.25, 0.50, and 0.75 g GP/kg diet, respectively and the group 5th, 6th, and 7th were fed on the basal diet plus 0.25, 0.50, and 0.75 g PA/kg diet. Results: Broiler body weight and gain at 21 and 42 days were increased (p<0.05) with diets supplemented with GP and PA. Red blood cells and hemoglobin were improved in chickens fed diets enriched with GP. Broiler chickens received diets containing either GP or PA recorded the higher values (p<0.05) of total protein, globulin, high-density lipoprotein, immunoglobulin M (IgM), and IgG, superoxide dismutase and total antioxidant capacity; while, blood total cholesterol, low-density lipoprotein, aspartate-aminotransferase, and malondialdehyde were lowered (p<0.05) compared to control-diet. Liver and immune-related organs weight were improved (p<0.05) in broilers fed diet supplemented with GP and PA. Conclusion: Feeding of GP or PA in diet had positive effects on performance traits and immunological, antioxidant and physiological status of broilers. Thus, the use of tested feed additives as an eco-friendly alternative to antibiotics produced a positive effect on animal health.

• 한국가금학회지
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• 제34권3호
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• pp.173-180
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• 2007

본 연구는 CLA 전구체 및 정제 부산물의 급여가 산란계의 산란율, 계란 품질 및 면역 관련 혈액학적 지표에 미치는 영향에 대하여 알아보기 위하여 시험을 실시하였다. 사양 시험은 32주령 ISA Brown 252수를 공시하였으며, 5주간 사양 시험을 실시하였다. 실험 설계는 1) CON(basal diet + oat 1% + soybean oil 1%), 2) WOS(basal diet + whey 0.5% + oat 0.5% + soybean oil 1%), 3) WS(basal diet+ whey 1% + soybean oil 1%), 4) WOCLA(basal diet + whey 0.5% + oat 0.5% + CLA produce 1%), 5) WCLA(basal diet + whey 1% + CLA produce 1%) 및 6) FOCLA(basal diet + Fish oil 0.5% + oat 1% + CLA produce 0.5%)로 6개 처리를 하여 처리당 7반복, 반복당 6수씩 완전 임의 배치하였다. 난각 두께는 시험 4주에서 WOS 및 FOCLA 처리구가 WOCLA 및 WCLA 처리구와 비교하여 유의적으로 높게 나타났으며(P<0.05), 난중은 시험 4주에서 WOS 및 FOCLA 처리구가 WS 및 WCLA 처리구와 비교하여 유의적으로 높게 나타났다(P<0.05). 난황고, 난황색 및 호우 유닛에 있어서 FOCLA 첨가구는 대조구와 비교하여 높게 나타났으며, 종료 시 IgG에 있어서 WOS 및 FOCLA 처리구가 대조구, WS 및 WOCLA 처리구와 비교하여 유의적으로 높게 나타났다(P<0.05). 결론적으로 어유, 정제 부산물 및 귀리의 첨가는 난각 두께, 난황고, 난황색 및 호우 유닛을 개선시키는 효과를 보여주었으며, CLA 전구체의 첨가 급여는 혈액 내 IgG의 함량을 대조구와 비교하여 증가시켰다.

• Asian-Australasian Journal of Animal Sciences
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• 제33권3호
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• pp.424-435
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• 2020

Objective: The study was conducted to investigate variations in the immunophysiological responses to exercise-induced stress in Jeju and Thoroughbred horses. Methods: Blood samples were collected from the jugular veins of adult Jeju (n = 5) and Thoroughbred (n = 5) horses before and after 30 min of exercise. The hematological, biochemical, and immunological profiles of the blood samples were analyzed. Blood smears were stained and observed under a microscope. The concentration of cell-free (cf) DNA in the plasma was determined using real time polymerase chain reaction (PCR). Peripheral blood mononuclear cells (PBMCs) and polymorphonuclear cells were separated using Polymorphprep, and the expression of various stress-related and chemokine receptor genes was measured using reverse transcriptase (RT) and real-time PCR. Results: After exercise, Jeju and Thoroughbred horses displayed stress responses with significantly increased rectal temperatures, cortisol levels, and muscle catabolism-associated metabolites. Red blood cell indices were significantly higher in Thoroughbred horses than in Jeju horses after exercise. In addition, exercise-induced stress triggered the formation of neutrophil extracellular traps (NETs) and reduced platelet counts in Jeju horses but not in Thoroughbred horses. Heat shock protein 72 and heat shock protein family A (Hsp70) member 6 expression is rapidly modulated in response to exercise-induced stress in the PBMCs of Jeju horses. The expression of CXC chemokine receptor 4 in PBMCs was higher in Thoroughbred horses than in Jeju horses after exercise. Conclusion: In summary, the different immunophysiological responses of Jeju and Thoroughbred horses explain the differences in the physiological and anatomical properties of the two breeds. The physiology of Thoroughbred horses makes them suitable for racing as they are less sensitive to exercise-induced stress compared to that of Jeju horses. This study provides a basis for investigating the link between exercise-induced stresses and the physiological alteration of horses. Hence, our findings show that some of assessed parameters could be used to determine the endurance performance of horses.

• Asian-Australasian Journal of Animal Sciences
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• 제14권1호
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• pp.21-29
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• 2001

An experiment was conducted with twenty crossbred male lambs to assess the effect of cotton (Gossypium) seed meal (CSM) on blood constituents and immunity. Lambs were randomly assigned to a reference diet (30% deoiled peanut meal, DPNM) and four test diets containing 40% of either raw, 45 minutes cooked, 1% $Ca(OH)_2$ and iron (1 free gossy-pol, FG : 0.3 Fe) treated CSM (replacing approximately 50%, reference concentrate mixture). These isonitrogenous and isocaloric concentrate mixtures were fed to meet 80% of protein requirements (NRC, 1985) along with ad lib maize hay for 180 days. Blood was collected at 60, 120 and 180 days post feeding. The lambs were sensitized with Brucella abortus S99 antigen after 140 days and were subjected to ELISA and delayed type hypersensitivity. Blood haemoglobin, erythrocyte count, leucocyte count, total protein, total albumin, total globulin, urea, creatinine concentration and aspartate aminotransferase activity in lambs fed on raw or processed CSM were comparable to the values of reference lambs. The higher (p<0.01) blood glucose levels observed in CSM fed lambs at 60 days of feeding was latter reduced to the levels comparable with those on reference diet at 120 and 180 days of feeding. The alanine amino transferase activity was lower in lambs fed raw and cooked CSM containing diets at 120 and 180 days of feeding. A marginal increase in serum iron and alkaline posphatase activity was observed in iron treated group and raw CSM fed lambs, respectively. The humoral immune response and DTH reactivity was lower (p<0.05) in lambs fed raw CSM (consuming 302.83 mg FG/day). Cooking, $Ca(OH)_2$ and iron treatment of raw CSM showed a positive response in alleviating the suppression of immune response owing to the reduced consumption of FG by 40.19, 17.40% and 26.73%, respectively in these diets. The present study thus indicated that consumption of 40% raw CSM (302.83 mg FG/day) though did not affect majority of the haematological and blood biochemical parameters, but markedly suppressed the immune mechanism of lambs.

• IMMUNE NETWORK
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• 제20권6호
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• pp.48.1-48.11
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• 2020

Hyperprogressive disease (HPD) is a distinct pattern of progression characterized by acceleration of tumor growth after treatment with anti-PD-1/PD-L1 Abs. However, the immunological characteristics have not been fully elucidated in patients with HPD. We prospectively recruited patients with metastatic non-small cell lung cancer treated with anti-PD-1/PD-L1 Abs between April 2015 and April 2018, and collected peripheral blood before treatment and 7-days post-treatment. HPD was defined as ≥2-fold increase in both tumor growth kinetics and tumor growth rate between pre-treatment and post-treatment. Peripheral blood mononuclear cells were analyzed by multi-color flow cytometry to phenotype the immune cells. Of 115 patients, 19 (16.5%) developed HPD, 52 experienced durable clinical benefit (DCB; partial response or stable disease ≥6 months), and 44 experienced non-hyperprogressive progression (NHPD). Patients with HPD had significantly lower progression-free survival (p<0.001) and overall survival (p<0.001). When peripheral blood immune cells were examined, the pre-treatment frequency of CD39⁺ cells among CD8⁺ T cells was significantly higher in patients with HPD compared to those with NHPD, although it showed borderline significance to predict HPD. Other parameters regarding regulatory T cells or myeloid derived suppressor cells did not significantly differ among patient groups. Our findings suggest high pre-treatment frequency of CD39⁺CD8⁺ T cells might be a characteristic of HPD. Further investigations in a larger cohort are needed to confirm our results and better delineate the immune landscape of HPD.

• Toxicological Research
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• 제21권2호
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• pp.121-128
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• 2005

Economic animal husbandry workers exposed to organic dust can be suffered from immunologic disorders. Our study was to determine immunological parameters related with occurrence of respiratory allergic diseases to animal husbandry workers in Korea for the first time. Peripheral blood were obtained from twenty-five pig barn workers, forty-nine chicken farming workers and fifty-one non-agricultural control workers. Significantly upregulated plasma IgE level was observed with pig-barn workers than that of chicken farming workers or healthy community control subjects. Furthermore, level of histamine, a hallmark of allergy induction, was upregulated in the pig and chicken farming workers in comparison with that of the control subjects. Downregulation of $IFN_\gamma$ and $TNF_{\alpha}$ production from T cells was apparent in the animal husbandry workers compared with the control subjects. Meanwhile, T cells collected from the pig barn workers demonstrated significantly higher production of IL-4 and IL-10 than the other groups. There were also alterations in IgG subclass distribution. In conclusion, immunological modulation probably leading to occupational allergic diseases can be occurred in the economic animal husbandry workers and the pig barn workers could be the most risky group to the work-related allergic disease.

• 대한한방소아과학회지
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• 제22권3호
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• pp.105-137
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• 2008

Objectives : The purpose of this study is to investigate the effect of Gupoongjeseuptang (GPJST) on atopic dermatitis by in vivo experiment using NC/Nga atopic dermatitis mouse, which has histological and clinical similarities to the atopic dermatitis of human. Methods : To investigate the effect of GPJST on atopic dermatifis, we evaluated atopic dermatitis-like skin lesions by clinical skin index and analyzed immunological parameters in peripheral blood mononuclear cells (PBMCs), splenocytes, draining lymph node (DLN) and performed skin histology in ears and dorsal skin of atopic dermatitis-like skin NC/Nga mouse in vivo. Results : In vivo, clinical skin severity score were significantly lower in GPJST group than control group. IgE, IL-6, $TNF-{\alpha}$, IgG1, IgM, IgG2a and IgG2b levels in serum decreased remarkably in GPJST group than control group. Also, total absolute number of $CD3^+CD69^+$, and $CCR3^+$ cells recovered as normal in PBMCs and $CD3^+$, $CD3^+CD69^+$ decreased significantly compared with control group in isolated DLN from NC/Nga mouse and total absolute number of $CD11b^+Gr-1^+$, $CCR3^+CD3^+$ in dorsal skin of NC/Nga mouse decreased by GPJST. We analyzed ear and neck-back skin after biopsy and dyeing by hematoxyline/eosin (H&E) and toluidine staining (mast cells marker) and obtained results that GPJST are very effective to histological symptoms (dermal and epidermal thickening, hyperkeratosis and inflammatory cell (CD4, $CCR3^+$) infiltration). Conclusions : This study demonstrates immunological activity of GPJST on atopic dermatitis-like model mice.

• Journal of Animal Science and Technology
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• 제48권1호
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• pp.9-14
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• 2006

본 시험은 유질에 영향을 미치는 체세포수에 대하여 영향을 미칠 것으로 생각되는 면역물질에 대한 환경효과와 유전모수를 추정하고자 축산연구소에서 사육하고 있는 착유우를 공시하여 이들의 혈액과 우유 시료를 채취하여 조사 분석하였다. 분만산차는 체세포수와 호중구에 영향을 미치는 것으로 나타났고(p<0.01), 비유기는 체세포수와 IgG, 호중구에 영향을 미치는 것으로 나타났다. 하지만 코티솔과 림프구는 산차와 유기에 의하여 영향을 받지 않는 것으로 나타났다. 체세포수의 산차간 비교에서 1, 2, 3 산차간에는 차이가 없었으나 4산차 이상에서는 높아지는 경향이 있어 3산차 이하와 비교 할 시에 유의적인 차이가 있었고 유기별 비교에서는 비유초기(90일 이하)와 비유후기(181일 이후)가 비유중기(91～180) 보다 높게 나타났다. 코티솔은 체세포수의 산차 성적과 마찬가지로 3산차 이하와 4산차 이상 간에 차이가 있었고 비유기별 성적에서는 높아지는 것처럼 보이나 차이는 없었다. 면역물질인 IgG는 1산차와 4산차 이상의 비교에서만 차이가 있었고 유기에서는 비유 초기가 비유 중기와 후기에 비하여 높은 결과를 보였고 림프구는 1산차와 4산차 이상 간에 차이가 있었으나 유기간에는 차이가 없었다. 호중구는 산차가 증가할수록 감소하는 경향으로 2산차 이하와 3산차 이상간에 차이가 있었으며 비유중기 까지는 차이가 없었고 비유 후기에는 높게 나타났다. 체세포수의 유전력은 낮은 수준이지만 유질 개량을 위하여 의미 있을 정도로 높게 추정되었으며 코티솔, 림프구와 부의 높은 유전상관을 보였다. 코티솔의 유전력은 비교적 낮은 수준이었고 림프구, 호중구간의 정의 상관관계를 보였다. IgG의 유전력은 중 정도를 보였으며 림프구와 호중구와는 높은 부의 유전상관을 보였다. 림프구는 높은 유전력을 보였고 호중구와도 높은 유전적 상관을 보였다. 호중구는 체세포수와 비슷한 수준의 유전력을 나타내었다. 이상의 연구 결과에서 코티솔, IgG, 림프구, 호중구가 체세포수와 유전적으로 높은 상관관계를 가지고 있어 우유 품질 개선에 중요한 형질임을 알 수 있었다. 또한 농가의 사육규모가 증가하고 두 당 생산성이 더욱 증가함에 따라 생산성과 질병을 동시에 고려하는 젖소개량 관련 연구가 수행되어야 할 것으로 사료된다.