• Asian Pacific Journal of Cancer Prevention
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• v.16 no.10
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• pp.4409-4419
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• 2015

The Bhopal gas tragedy involving methyl isocyanate (MIC) is one of the most horrific industrial accidents in recent decades. We investigated the genotoxic effects of MIC in long-term survivors and their offspring born after the 1984 occurrence. There are a few cytogenetic reports showing genetic damage in the MIC-exposed survivors, but there is no information about the associated cancer risk. The same is true about offspring. For the first time, we here assessed the micronucleus (MN) frequency using cytokinesis-blocked micronucleus (CBMN) assay to predict cancer risk in the MIC-affected population of Bhopal. A total of 92 healthy volunteers (46 MIC-affected and 46 controls) from Bhopal and various regions of India were studied taking gender and age into consideration. Binucleated lymphocytes with micronuclei (BNMN), total number of micronuclei in lymphocytes (MNL), and nuclear division index (NDI) frequencies and their relationship to age, gender and several lifestyle variabilities (smoking, alcohol consumption and tobacco-chewing) were investigated. Our observations showed relatively higher BNMN and MNL (P<0.05) in the MIC-affected than in the controls. Exposed females (EF) exhibited significantly higher BNMN and MNL (P<0.01) than their unexposed counterparts. Similarly, female offspring of the exposed (FOE) also suffered higher BNMN and MNL (P<0.05) than in controls. A significant reduction in NDI (P<0.05) was found only in EF. The affected group of non-smokers and non-alcoholics featured a higher frequency of BNMN and MNL than the control group of non-smokers and non-alcoholics (P<0.01). Similarly, the affected group of tobacco chewers showed significantly higher BNMN and MNL (P<0.001) than the non-chewers. Amongst the affected, smoking and alcohol consumption were not associated with statistically significant differences in BNMN, MNL and NDI. Nevertheless, tobacco-chewing had a preponderant effect with respect to MNL. A reasonable correlation between MNL and lifestyle habits (smoking, alcohol consumption and tobacco-chewing) was observed only in the controls. Our results suggest that EF and FOE are more susceptible to cancer development, as compared to EM and MOE. The genotoxic outcome detected in FOE reflects their parental exposure to MIC. Briefly, the observed cytogenetic damage to the MIC-affected could contribute to cancer risk, especially in the EF and FOE.

• The Journal of the Korea Contents Association
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• v.11 no.10
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• pp.355-369
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• 2011

The purpose of this study was to determine the subjective symptoms of respiratory disease and factors related to prevalent status among dental technicians by analyzing the questionnaire data from 300 dental technicians in Seoul and GyeongGi-Do, using SPSS WIN 18.0 program. The results are followings; 1. There were significant differences among subjects in the normal complaints rate for cough by presence smoking, total career, and work days per week. 2. There was significant difference among subjects in the normal complaints rate for sputum only by work days per week.(P<0.05) 3. There were significant differences among subjects in the normal complaints rate for continuance of sputum or congestion by work days per week and required measures for improvement in pain. 4. There were significant differences among subjects in the normal complaints rates for blocked nose and coryza by sex, age, work days per week and average length of breaking time during working (P<0.05). 5. There were significant differences among subjects in the normal complaints rates for shortness of breath by sex, exercise frequency, average length of exercise, main job. 6. There was significant difference among subjects in the experience rate due to symptom of pulmonary disease only by main job.(P<0.05).

• Journal of the Korean Society of Food Science and Nutrition
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• v.41 no.7
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• pp.901-906
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• 2012

The purpose of this study was to elucidate the anti-proliferative effect and the mechanisms underlying apoptosis induced by a methanol extracts from Ishige sinicola (ISE) in HeLa cells. ISE treatment for 24 hr significantly inhibited cell viability in a dose-dependent manner. Apoptosis was detected by Hoechst 33258 staining and an annexin V/PI assay after 24 hr treatment. Moreover, ISE treatment triggered the cleavage of caspase-8, -9, -3, and poly(ADP-ribose) polymerase (PARP) in dose-dependent and time-dependent manners. In addition, z-VAD-fmk, a general caspase inhibitor, blocked ISE-induced cell death. Taken together, these results suggest that ISE-induced apoptosis is mediated by the activation of a caspase cascade in HeLa cells.

• Journal of the Korean Society of Food Science and Nutrition
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• v.44 no.5
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• pp.649-656
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• 2015

The protective effect of zinc against cortisol-induced cell injury was examined in rainbow trout gill epithelial cells. Cells exposed to cortisol for 24 h showed increased leakage of lactate dehydrogenase (LDH) as well as decreased cell viability in a dose-dependent manner. Treatment with zinc ($100{\mu}M$ $ZnSO_4$) reduced the severity of both LDH release and cell death as well as protected cells against cortisol-induced caspase-3 activation, indicating reduction of apoptosis. Cortisol-induced cell death, leakage of LDH, and caspase-3 activation were blocked by the glucocorticoid receptor antagonist Mifepristone (RU-486), suggesting that cell injury was cortisol-dependent. In addition, we studied the effect of zinc on the expression of antioxidant genes such as metallothionein A (MTA), metallothionein B (MTB), glutathione-S-transferase (GST), and glucose-6-phosphate dehydrogenase (G6PD) during cortisol-induced cell injury. MTA, MTB, GST, and G6PD mRNA levels increased after treatment with zinc or cortisol, separately or in combination. Higher mRNA levels of MTA, MTB, GST, and G6PD were detected when cells were treated with $100{\mu}M$ $ZnSO_4$ and $1{\mu}M$ cortisol in combination at the same time compared to treatment with zinc or cortisol separately. Cells treated with zinc showed increased intracellular free zinc concentrations, and this response was significantly enhanced in cells treated with cortisol and zinc. In conclusion, zinc treatment inhibited cortisol-induced cytotoxicity and apoptosis through indirect antioxidant action.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.3 no.1
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• pp.1-12
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• 1983

The wave and hydrologic climate at a beach location are everchanging and coastal engineers have always been interested in the deformation of the natural beach caused by wave action over short or long interval of time. The drift of sand on a beach particulary manifests itself when blocked construction of seawalls, jetties, breakerwaters and groins etc.${\cdots}$ For this reason, the understanding and evaluation of littoral drift has been especially important to the coastal engineers. The resulting of the sand drift movement, such as the type of beach profile, width of beach, storage volume of the littoral sand over the profile are rapid everchange. We have studied the geological changes due to the littoral drift on a beach with field investigation and model tests in laboratory. But, it is impossible to make quantative correct analysis because of the factors are everchange and complicate. And then, most of study are incline to qualitative analysis. In this paper, authors studied mainly on the transition of beach profile and sediment storage on the profile using statistical field data as qualitative analytical method. The used theoretical beach transition model by Sonu and Beek have developed to obtain the change of HAEUNDAE beach backed with seawall. Results of this study indicate that the transition model are useful in the analysis of beach profile changement and the littoral drift movement on the beach. Qualitative analysises for HAEUNDAE beach are as follows. 1) Transition sequence of profile has 4 major transition for one cycle. 2) Storage sediment model of beach profile by Sonu and Beek well coincided with HAEUNDAE beach. 3) Seasonal cycle has ill-balanced process for the 5-yr. investigation.

• Journal of Korean Society of Coastal and Ocean Engineers
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• v.22 no.2
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• pp.67-72
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• 2010

In this study, the impact on the circulating water system has been analyzed due to an installation of helical turbine to develop hydro-kinetic energy at the discharge channel of the power plant. Numerical simulations of velocity and pressure variations have been performed when one set of $3.6\;m\;{\times}\;1.5\;m$ sized helical turbine is installed at the outlet of discharge culvert. In case of mean sea level, change of downstream water surface elevation does not affect upstream elevation of the weir because its propagation is blocked by the seal well weir. However in case of highest high water level, change of downstream elevation affects upstream elevation because flow pattern in discharge culvert becomes the full pipe flow with submerged weir. Although an unstable pressure change occurs in upstream of the weir during the intial 10 minutes after beginning of the discharge, it becomes stable after that time. In addition, a rise of water surface elevation by 0.2 m is observed but it is concluded that it hardly affects the safety of circulating water pump (CWP) although its required power is increased more or less. Therefore, the increase of required power of CWP needs to be considered for evaluation of the helical turbine applicability.

• Journal of Korea Water Resources Association
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• v.47 no.3
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• pp.297-306
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• 2014

In this study, EFDC (Environmental Fluid Dynamics Code) model was used to simulate the salinity distribution for sea water inflow and rainfall runoff. The flowrate was given to the boundary conditions, which can be calculated by areal-specific flowrate method from the measured flowrate of the representative outfall. The boundary condition of the water elevation can be obtained from the hourly tidal elevation. The flowrate from the outfall can be calculated using the condition of the 245 mm raifall. The simulation results showed that at Sites 1~2 and the Mangsan island (Site 4) the salinity becomes 0 ppt after the rainfall. However, the salinity is 30 ppt when there is no rainfall. Time series of the salinity changes were compared with the measured data from January 1 to December 31, 2010 at the four sites (Site 2~5) of Yongwon channel. Lower salinities are shown at the inner sites of Yongwon channel (Site 1~4) and the sites of Songjeong river (Site 7~8). The intensive investigation near the Mangsan island showed that the changes of salinity were 21.9~28.8 ppt after the rainfall of 17 mm and those of the salinity were 2.33~8.05 ppt after the cumulative rainfall of 160.5 mm. This means that the sea water circulation is blocked in Yongwon channel, and the salinity becomes lower rapidly after the heavy rain.

• Journal of Korean Medicine Rehabilitation
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• v.21 no.2
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• pp.211-226
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• 2011

Objectives : The purpose of this study is to establish literatural evidence about traditional Korean medical physiotherapy through literatural review. Methods : Applicable paragraphs which were related to the traditional Korean medical physiotherapy were phrased from in "Hwang-je-nae-gyung(黃帝內經)", "Yumun-sa-chin(儒門事親)", "Geum-guei-yo-ryack(金匱要略)", "Eui-hack-ip-mun(醫學入門)", "Gyung-ack-jeon-seo(景岳全書)", "Eui-hack-gang-mock(醫學綱目)", "Dong-eui-bo-gam(東醫寶鑑)" where were archiving of Oriental or Korean medicine literatures. Searched paragraphs were analysed for establishing historical and theoretical bases of Korean medical physiotherapy. Results : 1. Theromtherapy is originated from yu(熨), hot hand, warm, water bath, warm earth, fumigation, lamp, torchlight and brazier. Matching indications are various musculoskeletal pain and diseases, skin diseases such as chronic inflammation, frostbite, gynecological and urogenital diseases such as uterine myoma, lower abdominal pain, metrorrhagia, breast diseases, digestive tract diseases such as vomiting, diarrhea, stomachache and also it treats cardiovascular disease. Other matching indications are sunstroke, rhinorrhea, psycosis and anal pain. And it used helping acupuncture therapy. 2. Cryotherapy is originated from lengfu(冷敷), lengtie(冷貼), cold stone and cold water. Matching indications are various fever disease, skin diseases such as acute inflammation, wound, fever skin disease, otorhinolaryngological diseases such as acute sore throat, otitis, stomatitis, nasal bleeding and contusions, abrasions, burn and dysuria. In addition, it treats extremity coldness, chillness, stomachache caused to accumulate fever. 3. Physiotherapy by circulating meridian system is originated from manipulation such as pushing, massage and beating to therapy point. Matching indications are musculoskeletal diseases such as various muscular pain, heavy sensation, convulsion, urogenital diseases such as dysuria, lower abdomen pain, digestive tract diseases such as stomachache, and paralysis due to blocked meridian system. And other matching indications are psycosis, leprosy, hypoesthesia, acute stroke. In addition it helps other therapy methods. In addition, the power of manipulation classify to strengthen and reduce. 4. Physiotherapy by balancing meridian system is originated from sunshine, magnet, horn, bamboo and tube. Sunshine helps improving self's care and cure inflammatory skin disease. Magnet's matching indications are paralysis, various injuries, abscess, fever and eye disease. Cupping therapy treats to skin diseases and internal diseases such as tuberculosis. Conclusions : Traditional Korean medical physiotherapy was widely used in traditional Korean medicine since ancient time based on the traditional Korean medical principle and anatomical knowledge. With modern physiotherapy and traditional Korean medicine together, effects of treatment are enhanced. And traditional Korean medical physiotherapy is appropriated to world physiotherapy's expectation.

• Journal of Ginseng Research
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• v.38 no.4
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• pp.256-263
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• 2014

Background: Korean Red Ginseng (KRG) is known to have antianxiety properties. This study was conducted to investigate the anxiolytic effects of KRG extract (KRGE) during ethanol withdrawal (EW) and the involvement of the mesoamygdaloid dopamine (DA) system in it. Methods: Rats were treated with 3 g/kg/d of ethanol for 28 d, and subjected to 3 d of withdrawal. During EW, KRGE (20 mg/kg/d or 60 mg/kg/d, p.o.) was given to rats once/d for 3 d. Thirty min after the final dose of KRGE, anxiety-like behavior was evaluated in an elevated plus maze (EPM), and plasma corticosterone (CORT) levels were determined by a radioimmunoassay (RIA). In addition, concentrations of DA and 3,4-dihydroxyphenylacetic acid (DOPAC) in the central nucleus of the amygdala (CeA) were also measured by high performance liquid chromatography (HPLC). Results: The EPM test and RIA revealed KRGE inhibited anxiety-like behavior and the over secretion of plasma CORT during EW. Furthermore, the behavioral effect was blocked by a selective DA D2 receptor (D2R) antagonist (eticlopride) but not by a selective DA D1 receptor (D1R) antagonist (SCH23390). HPLC analyses showed KRGE reversed EW-induced decreases of DA and DOPAC in a dose-dependent way. Additionally, Western blotting and real-time polymerase chain reaction (PCR) assays showed that KRGE prevented the EW-induced reductions in tyrosine hydroxylase (TH) protein expression in the CeA and TH mRNA expression in the ventral tegmental area (VTA). Conclusion: These results suggest that KRGE has anxiolytic effects during EW by improving the mesoamygdaloid DA system.

• Journal of Ginseng Research
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• v.40 no.4
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• pp.437-444
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• 2016

Background: Although Korean Red Ginseng (KRG) has been traditionally used for a long time, its anti-inflammatory role and underlying molecular and cellular mechanisms have been poorly understood. In this study, the anti-inflammatory roles of KRG-derived components, namely, water extract (KRG-WE), saponin fraction (KRG-SF), and nonsaponin fraction (KRG-NSF), were investigated. Methods: To check saponin levels in the test fractions, KRG-WE, KRG-NSF, and KRG-SF were analyzed using high-performance liquid chromatography. The anti-inflammatory roles and underlying cellular and molecular mechanisms of these components were investigated using a macrophage-like cell line (RAW264.7 cells) and an acute gastritis model in mice. Results: Of the tested fractions, KGR-SF (but not KRG-NSF and KRG-WE) markedly inhibited the viability of RAW264.7 cells, and splenocytes at more than 500 mg/mL significantly suppressed NO production at $100{\mu}g/mL$, diminished mRNA expression of inflammatory genes such as inducible nitric oxide synthase, cyclooxygenase-2, tumor necrosis factor-${\alpha}$, and interferon-${\beta}$ at $200{\mu}g/mL$, and completely blocked phagocytic uptake by RAW264.7 cells. All three fractions suppressed luciferase activity triggered by interferon regulatory factor 3 (IRF3), but not that triggered by activator protein-1 and nuclear factor-kappa B. Phospho-IRF3 and phospho-TBK1 were simultaneously decreased in KRG-SF. Interestingly, all these fractions, when orally administered, clearly ameliorated the symptoms of gastric ulcer in HCl/ethanol-induced gastritis mice. Conclusion: These results suggest that KRG-WE, KRG-NSF, and KRG-SF might have anti-inflammatory properties, mostly because of the suppression of the IRF3 pathway.