• Korean Journal of Medicinal Crop Science
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• v.15 no.6
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• pp.451-456
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• 2007

Curcumin, a polyphenolic antioxidant purified from turmeric, has been known to possess various biological activities such as anti-oxidative, anti-inflammatory and anti-cancer effects. In this study, we have explored anti-inflammatory effect of curcumin using Gram (-) bacterium-derived endotoxin (lipopolysaccharide: LPS) and macrophage cell line RAW264.7. Curcumin suppressed NO production in LPS-activated RAW264.7 cells in a dose-dependent manner, Curcumin also blocked the activation of $NF-{\kappa}B$ but not AP-1 according to luciferase assay. Furthermore, this compound suppressed the phosphorylation of a series of intracellular signaling components such as Src, JAK-2, Akt, IKK and $I{\kappa}B{\alpha}$ under LPS stimulation in a time dependent manner, Therefore, our data suggest that curcumin was able to protect the host from Gram(-) bacterial-infection-mediated inflammatory symptoms.

• Journal of Physiology & Pathology in Korean Medicine
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• v.26 no.6
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• pp.922-928
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• 2012

Sulforaphane [1-isothiocyanato-4-(methylsulfinyl)-butane] is one of the most abundant isothiocyanates in some cruciferous vegetables, especially broccoli. Sulforaphaene has been shown to exhibit many pharmacological activities, including anti-oxidant, anti-inflammatory and anti-microbial activities. However, the anti-skin photoaging effects of sulforaphane have not yet been reported. In the present study, we investigated the inhibitory effects of sulforaphane on MMP-1 and -3 expressions of the human dermal fibroblasts via various in vitro experiments and elucidated the pathways of inhibition. Western blot analysis and real-time PCR revealed sulfiraphane inhibited UVB-induced MMP-1 and -3 expressions in a dose-dependent manner. UVB strongly activated nuclear factor-${\kappa}B$ (NF-${\kappa}B$) activity, which was determined by NF-${\kappa}B$ DNA binding activity. UVB-induced NF-${\kappa}B$ activation and MMP expression were completely blocked by sulforphane. These findings suggest that sulforaphane could prevent UVB-induced MMPs expressions through inhibition of NF-${\kappa}B$ activation.

• Korean Journal of Oriental Medicine
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• v.4 no.1 s.4
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• pp.99-114
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• 1998

The effect of herbal medicine on glutamate mediated neurotoxicity was studied in mouse neurons in primary culture. Immature cerebral cortex neurons (ED14) were maintained for up to 2 weeks in vitro, and we investigated the expression pattern of neuron differentiation and cytotoxicity of cell death, including LDH activity. Neuronal maturation initiated on day 7 and the susceptibility to glutamate-induced cell death was highly sensitive on Day 11 (Fig. 1). Thus, the exposure of the neurons to glutamate caused a dose$(0.1mM{\sim}1mM)$ and time$(4h{\sim}24h)$-dependent neurotoxicity(Fig. 4). Glutamate-induced neurodegeneration was prevented by Shipchondaebotang(SD), Yollyounggobondan(YG), Yugmijihwangwon(YJ) and the death of neurons exposed to glutamate was blocked by the NMDA receptor antagonist MK-801 (Fig. 5).

• Proceedings of the Korean Vacuum Society Conference
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• 2013.08a
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• pp.219-219
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• 2013

The effect of an electron blocking layer (EBL) on V-I curves in GaN/InGaN multiple quantum well is investigated. For the first time, we found that curves were intersected at 3.012 V and analyzed the reason for intersection. The forward voltage in LEDs with an p-AlGaN EBL is larger than without p-AlGaN EBL at low injection current because the Mg doping efficiency for p-GaN layer was higher than that of p-AlGaN layer. However, the forward voltage in LEDs with an p-AlGaN EBL is smaller than without p-AlGaN EBL at high injection current because the carriers overflow from the active layer when injection current increases in LEDs without p-AlGaN EBL and in case of LED with p-AlGaN EBL, the carriers are blocked by EBL.

• Molecules and Cells
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• v.27 no.4
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• pp.403-408
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• 2009

Skeletal muscle regeneration is a highly orchestrated process initiated by activation of adult muscle satellite cells. Upon muscle injury, the inflammatory process is always accompanied by muscle regeneration. Leukotriene $B_4$ is one of the essential inflammatory mediators. We isolated and cultured primary satellite cells. RT-PCR showed that myoblasts expressed mRNA for $LTB_4$ receptors BLT1 and BLT2, and $LTB_4$ promoted myoblast proliferation and fusion. Quantitative real-time PCR and immunoblotting showed that $LTB_4$ treatment expedited the expression process of differentiation markers MyoD and M-cadherin. U-75302, a specific BLT1 inhibitor, but not LY2552833, a specific BLT2 inhibitor, blocked proliferation and differentiation of myoblasts induced by $LTB_4$, which implies the involvement of the BLT1 pathway. Overall, the data suggest that $LTB_4$ contributes to muscle regeneration by accelerating proliferation and differentiation of satellite cells.

• Molecules and Cells
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• v.40 no.5
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• pp.371-377
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• 2017

Despite the importance of the receptor activator of nuclear factor (NF)-kappaB ligand (RANKL)-RANK signaling mechanisms on osteoclast differentiation, little has been studied on how RANK expression is regulated or what regulates its expression during osteoclastogenesis. We show here that insulin signaling increases RANK expression, thus enhancing osteoclast differentiation by RANKL. Insulin stimulation induced RANK gene expression in time- and dose-dependent manners and insulin receptor shRNA completely abolished RANK expression induced by insulin in bone marrow-derived monocyte/macrophage cells (BMMs). Moreover, the addition of insulin in the presence of RANKL promoted RANK expression. The ability of insulin to regulate RANK expression depends on extracellular signal-regulated kinase 1/2 (ERK1/2) since only PD98059, an ERK1/2 inhibitor, specifically inhibited its expression by insulin. However, the RANK expression by RANKL was blocked by all three mitogen-activated protein (MAP) kinases inhibitors. The activation of RANK increased differentiation of BMMs into tartrate-resistant acid phosphatase-positive ($TRAP^+$) osteoclasts as well as the expression of dendritic cell-specific transmembrane protein (DC-STAMP) and d2 isoform of vacuolar ($H^+$) ATPase (v-ATPase) Vo domain (Atp6v0d2), genes critical for osteoclastic cell-cell fusion. Collectively, these results suggest that insulin induces RANK expression via ERK1/2, which contributes to the enhancement of osteoclast differentiation.

• Nuclear Engineering and Technology
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• v.53 no.9
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• pp.2847-2858
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• 2021

When a nuclear reactor with rectangular fuel assemblies runs for a long time, impurities and debris may be taken into coolant channels, which may cause flow blockage, and the blocked fuel assemblies might be destroyed. Therefore, the purpose of this study is to perform a thermal-hydraulic analysis of a rectangular fuel assembly by STAR-CCM+, under the condition of one subchannel with 80% blockage ratio. A rectangular fuel assembly of the International Atomic Energy Agency (IAEA) 10 MW material test reactor (MTR) is chosen. In view of the gasket material taken into the coolant channel is close to the single side of the coolant channel, in the flow blockage accident of the Oak Ridge Research Reactor (ORRR), a new blockage category called single side blockage is attempted. The blockage positions include inlet, middle and outlet, and the blockage is set as a cuboid. It is found by simulations that the blockage redistributes the mass flow rate, and large vortices appear locally. The peak temperature of the cladding is maximum, when the blockage is located at the single side of the coolant channel inlet, and no boiling occurs in all blockage cases. Moreover, as the height of the blockage increases, the damage caused by the blockage increases slightly.

• ETRI Journal
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• v.43 no.4
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• pp.660-673
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• 2021

To efficiently use frequency resources, the next 6th generation mobile communication technology must solve the problem of out-of-band emission (OoBE) of cyclic prefix (CP) orthogonal frequency division multiplexing (OFDM), which is not solved in 5th generation technology. This study describes a new zero insertion technique to replace an existing filtering scheme to solve this internal problem in OFDM signals. In the development of the proposed scheme, a precoder with a two-dimensional structure is first designed by generating a two-dimensional mapper and using the specialty of each matrix. A spectral shaping technique based on zero insertion instead of a long filter is proposed, so it can be applied not only to long OFDM symbols, but also very short ones. The proposed method shows that the transmitted signal is completely blocked at the bandwidth boundaries of signals according to the current standards, and it is confirmed that the proposed scheme is ideal with respect to bit error rate (BER) performance because its BER is the same as that of CP-OFDM. In addition, the proposed scheme can transformed into a real time structure through vectorizing process with minimal complexity.

• Journal of the Architectural Institute of Korea Planning & Design
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• v.35 no.1
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• pp.107-115
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• 2019

This research explores a historical trajectory of the Milwaukee Art Museum from its establishment to the third expansion over the last six decades. After established in 1957 by the architect, Eero Saarinen, this museum underwent three expansions led by three different architects including, David Kahler, Santiago Calatrava, and James Shields. Reasons for expanding the museum were varied. A lack of exhibition space, an absence of visual identity, and path interruption problem within urban contexts were the main factors of a series of expansion. Furthermore, this research points out that there are three issues in the process of the expansion. The first is connection issues between the downtown and the lakefront in which the museum has blocked the public flow. The second, there were controversies on the allocation of the expanded space among the main body of the decision including architects, curators, and stakeholders. The last one is relationships among architects. This is related to each architect's attitude toward the museum. Drawing on historical documents and interviews with the regional architects, I argue that the identity and values of the museum have changed over time through the expansions rather than having the invariable.

• Nuclear Engineering and Technology
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• v.54 no.1
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• pp.117-129
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• 2022

The downward discharge of the molten fuel to the lower structure of the fuel assembly could increase of the pressure drop and degrade of coolability of the assembly. To analyze the phenomena, experiments for the generation of the debris bed were conducted as LOF-DT series. Based on the debris bed in the LOF-DT, pressure drop experiment was conducted with intact and blocked component. Parametric study on the pressure drop was conducted by CFD. The LOF-DT experiments were conducted for the position and porosity of the debris bed. 85% of the debris were sedimented in the lower reflector, and 15% were in the nose piece, approximately. Porosity of the debris bed were about 0.7 and 0.85 in the lower reflector and nose piece, respectively. Pressure drop increased significantly with debris bed, especially in the lower reflector. More than 120 time of the pressure drop increased in the lower reflector, while only 10% increased in the nose piece. According to the parametric study, mass of the debris was the most important for pressure drop. The lower discharge phenomena could have a significant effect to the total pressure drop of the fuel assembly, approximately 10.8 times for the base case.