• Asian-Australasian Journal of Animal Sciences
• /
• v.14 no.4
• /
• pp.515-524
• /
• 2001

Two experiments using growing and finishing pigs (Landrace$\times$Duroc$\times$Yorkshire) were conducted to determine the effects of different levels of vitamins and trace minerals (VTM) on growth performance, nutrient digestibility, carcass characteristics and meat quality of growing-finishing pigs, and to evaluate the suitability of vitamin-mineral levels commonly used in the swine industry in Korea. A total of 120 three crossbred (Landrace$\times$Duroc$\times$Yorkshire) growing (Experiment I: 20.90 0.44 kg average initial body weight) and finishing (Experiment II: 53.55 0.97 kg average initial body weight) were used in 6 and 9 weeks feeding trials, respectively. Pigs were allotted on the basis of sex and weight to 5 treatments with 6 replications per treatment with 4 pigs per pen in a completely randomized block design. Treatments were: 1) control, 2) fat soluble vitamin 200% vs. control, 3) water soluble vitamin 200% vs. control, 4) trace mineral 50% vs. control, 5) trace mineral 200% vs. control. In experiment I, during the overall experimental period (0 to 6 weeks, 21 to 54 kg body weight), ADG of the 200% trace mineral supplemented group was significantly higher than that of the 50% trace mineral supplemented group. There were no significant differences among other the treatments. Digestibility of crude protein was lowest in mineral 50% supplemented group (p<0.05). Calcium digestibility was significantly higher in the 200% fat soluble vitamin supplemented group than in the other treatments (p<0.05). In experiment II, during the overall experimental period (0 to 9 weeks, 54 to 106 kg body weight), growth performance was not significantly affected by dietary vitamin and trace mineral levels. However, increasing level of water soluble vitamins at the level of 200% compared to control had a tendency to improve the overall growth performance. Overall carcass characteristics except for carcass length did not differ among pigs fed the dietary treatments. Loin eye area, pH, drip loss and shear force of meat were not affected by dietary vitamin and trace mineral levels. There was a trend for less fat content (%) in pork when the level of vitamin and trace mineral was increased, but the difference was not significant. Flavor score was the lowest in control and highest in the 200% fat soluble vitamin supplemented group (p<0.05). Juiciness of muscle was lower in the 200% fat soluble vitamin supplemented group than other dietary treatments, except for trace mineral 50% supplemented group (p<0.05). Based on these results, it is suggested that "typical" commercial levels of vitamin and trace minerals used by feed companies in Korea are sufficient to meet requirement for the maximum growth of growing-finishing pigs. Our results suggests that a reduction in trace mineral levels in commercial diets could be considered to reduce feed cost and nutrient excretion with economic and environmental benefits.

• The Journal of Korean Institute of Electromagnetic Engineering and Science
• /
• v.23 no.6
• /
• pp.691-699
• /
• 2012

In this paper, we present a Vt-DRO with a low phase noise, which is achieved by improving the coupling structure between the dielectric resonator and microstrip line. The Vt-DRO is a closed-loop type and is composed of 3 blocks; dielectric resonator, phase shifter, and amplifier. We propose a mathematical estimation method of phase noise, using the group delay of the resonator. By modifying the coupling structure between the dielectric resonator and microstrip line, we achieved a group delay of 53 nsec. For convenience of measurement, wafer probes were inserted at each stage to measure the S-parameters of each block. The measured S-parameter of the Vt-DRO satisfies the open-loop oscillation condition. The Vt-DRO was implemented by connecting the input and output of the designed open-loop to form a closed-loop. As a result, the phase noise of the Vt-DRO was measured as -132.7 dBc/Hz(@ 100 kHz offset frequency), which approximates the predicted result at the center frequency of 5.3 GHz. The tuning-range of the Vt-DRO is about 5 MHz for tuning voltage of 0~10 V and the power is 4.5 dBm. PFTN-FOM is -31 dBm.

• The Journal of the Korea Contents Association
• /
• v.10 no.2
• /
• pp.286-294
• /
• 2010

This study was performed to identify the cerebral network associated with sensation through the tickling stimulation, which is distinctive from the rest of other networks processing normal stimulation and to investigate the difference of laughing mechanism which is closely related to tickling using functional MRI(fMRI). A 16 healthy volunteers (mean age: 28.9) on a 3.0T MR scanner during two sensation conditions. Counterbalanced stimulus were presented across the participants, and the stimulation was used block design. Acquired data was analyzed by the statistical parametric mapping (SPM 99). Subject and group analysis was performed. Individual analysis showed the activation of somatic sensation area in both tasks and the tickling sensation test showed more activated area in the Wernicke's area(BA40) compared to the normal sensation. The group analysis result shows that under normal stimulations, both sides of somatosensory cortices(BA 1,2 and 3) were activated and under tickling stimulation, not only the cortices but also those huge activation on thalamus, cingulate gyrus and insular lobe were detected. When the tickling was stopped, significant activations were shown in right cingulate gyrus, left MFG area and left insular lobe. A cerebral area responsible for recognizing tickling sensation was examined and the primitive stimulation such as tickling is much closely related to laugh, which is an important factor for various social activities.

• Parasites, Hosts and Diseases
• /
• v.40 no.1
• /
• pp.45-54
• /
• 2002

Field trials evaluating selected commercially available mosquito traps variously baited with light, carbon dioxide, and/or octenol were conducted from 18-27 September 2000 in a malarious area near Paekyeon-ri (Tongil-Chon) and Camp Greaves in Paju County, Kyonggi Province, Republic of Korea. The host-seeking activity for common mosquito species, including the primary vector of Japanese encephalitis, Culex tritaeniorhynchus Giles. was determined using hourly aspirator collections from a human and propane lantern-baited Shannon trap doting hours when temperatures exceeded $15^{\circ}C$. The total number of mosquitoes and number of each species captured during the test was compared using a block design. Significant differences were observed for the total number of mosquitoes collected, such that, the Mosquito MagnetTM with octenol > Shannon trap > ABC light trap with light and dry ice > Miniature Black Light trap (manufactured by John W. Hock) $\geq$ New Jersey Trap > ABC light trap with light only. Significant differences in numbers collected among trapes were noted for several species including: Aedes vexans (Meigen), Anopheles lesteri Baisas and Hu. An. sinensis Weidemann, An. sineroides Yamada, An. yatsushiroensis Miyazaki. Culex pipiens pallets Coquillett L., Cx. orientalis Edwards and Cx. tritaeniorhynchus. Host-seeking activity for most common species showed a similar bimodal pattern. Results from these field trap evaluations can significantly enhance current vector and disease surveillance efforts especially for the primary vector of Japanese encephalitis, Cx. tritaeniorhunchus.

• Journal of Life Science
• /
• v.28 no.4
• /
• pp.488-495
• /
• 2018

The p53 gene plays a critical role in the transcriptional regulation of cellular response to stress, DNA damage, hypoxia, and tumor development. Keeping in mind the recently discovered manifold physiological functions of p53, its involvement in the regulation of cancer is not surprising. In about 50% of all human cancers, inactivation of p53's protein function occurs either through mutations in the gene itself or defects in the mechanisms that activate it. This disorder plays a crucial role in tumor evolution by allowing the evasion of a p53-dependent response. Many recent studies have focused on directly targeting p53 mutants by identifying selective, small molecular compounds to deplete them or to restore their tumor-suppressive function. These small molecules should effectively regulate various interactions while maintaining good drug-like properties. Among them, the discovery of the key p53-negative regulator, MDM2, has led to the design of new small molecule inhibitors that block the interaction between p53 and MDM2. Some of these small molecule compounds have now moved from proof-of-concept studies into clinical trials, with prospects for further, more personalized anti-carcinogenic medicines. Here, we review the structural and functional consequences of wild type and mutant p53 as well as the development of therapeutic agents that directly target this gene, and compounds that inhibit the interaction between it and MDM2.

• The Plant Pathology Journal
• /
• v.34 no.5
• /
• pp.381-392
• /
• 2018

Clavibacter michiganensis subsp. michiganesis (Cmm) is a quarantine-worthy pest in $M{\acute{e}}xico$. The implementation and validation of new technologies is necessary to reduce the time for bacterial detection in laboratory conditions and Raman spectroscopy is an ambitious technology that has all of the features needed to characterize and identify bacteria. Under controlled conditions a contagion process was induced with Cmm, the disease epidemiology was monitored. Micro-Raman spectroscopy ($532nm\;{\lambda}$ laser) technique was evaluated its performance at assisting on Cmm detection through its characteristic Raman spectrum fingerprint. Our experiment was conducted with tomato plants in a completely randomized block experimental design (13 plants ${\times}$ 4 rows). The Cmm infection was confirmed by 16S rDNA and plants showed symptoms from 48 to 72 h after inoculation, the evolution of the incidence and severity on plant population varied over time and it kept an aggregated spatial pattern. The contagion process reached 79% just 24 days after the epidemic was induced. Micro-Raman spectroscopy proved its speed, efficiency and usefulness as a non-destructive method for the preliminary detection of Cmm. Carotenoid specific bands with wavelengths at 1146 and $1510cm^{-1}$ were the distinguishable markers. Chemometric analyses showed the best performance by the implementation of PCA-LDA supervised classification algorithms applied over Raman spectrum data with 100% of performance in metrics of classifiers (sensitivity, specificity, accuracy, negative and positive predictive value) that allowed us to differentiate Cmm from other endophytic bacteria (Bacillus and Pantoea). The unsupervised KMeans algorithm showed good performance (100, 96, 98, 91 y 100%, respectively).

• Korean Journal of Poultry Science
• /
• v.33 no.2
• /
• pp.133-139
• /
• 2006

This study was conducted to investigate the effects of dietary supplementation of Biotite V on growth performance, nutrients digestibility and serum and meat cholesterol in broiler chickens. A total of four hundred and eighty Arbor Acre broilers (male, 2-d-old) were used in current feeding trial for 5 weeks. Broilers were allocated to three dietary treatments with eight replications per treatment and twenty broilers per pen according to a randomized complete block design. Dietary treatments included: 1) CON (basal diet), 2) BV200 (basal diet+200 mesh Biotite V 1.5%) and 3) BV325 (basal diet+325 mesh Biotite V 1.5%). During the first week of experiment, Biotite V increased weight gain in treatment groups compared to CON treatment (P<0.05). Through the entire experimental period, weight gain, feed intake and feed/gain had no significant differences among the treatments (P>0.05). Digestibilities of DM and N were also not affected when diets included Biotite V (P>0.05). No effects were observed in Biotite V supplemented treatments compared to CON treatment on abdominal fat weight, meat and serum cholesterol concentrations (P>0.05). In conclusion, supplementation of Biotite V in broiler diets has no influence on growth performance, nutrients digestibility and can not decrease the cholesterol concentrations in meat and serum.

• Korean Journal of Poultry Science
• /
• v.33 no.4
• /
• pp.317-321
• /
• 2006

The effects of dietary humic substances (HS) on egg Production and egg Quality were studied using 252(55-wk old) ISA brown laying hens. laying were divided into 21 groups of 12 hens each and seven groups (experimental units) were assigned to 1) CON (basal diet), 2) HS5 (basal diet 4- 5% humic substances) or 3) HS10 (basal diet +10% humic substances) in a completely randomized block design. Hens had free access to diets and water fur 6 wk. Egg Production and egg quality were monitored over the 6-wk Period. Results showed that 10% dietary HS decreased egg Production and yolk diameter (P<0.05) compared to CON. Egg weight and yolk cole. were improved (P<0.05) in HS10 compared to CON. Egg shell breaking strength was increased significantly (P<0.05) when hens were fed HS5 diet compared to the others. There were no effects of treatments on egg shell thickness, yolk index, albumen height and Haugh nit. The results suggest that the dietary supplementation of HS at 5% or 10% decreases egg Production, but HS at 5% can increase egg shell breaking strength. Hens fed 10% HS could increase egg weight and yolk color and decrease yolk diameter.

• Journal of Animal Environmental Science
• /
• v.8 no.1
• /
• pp.35-42
• /
• 2002

This study was carried out to determine the effect of chemical fertilizer and two fermented types of pig slurry on the total dry matter yield and quality of sorghum $\times$ sudangrass hybrid (Sorghum bicolor L. Moench) and chemical properties of soil after harvest in Jeju area. Three treatments consisted of chemical fertilizer (CF) 200kg 7kg Nha $^1$, aerobic fermented pig slurry (PS)200kg 7kg Nha $^1$, and aerobic fermented pig slurry treated with probiotics (PS+P) 200kg 7kg Nha $^1$were arranged in a randomized block design with three replications. The results obtained are summarized as follows. The heights of plant applied fermented pig slurry were slightly taller than those of plants applied chemical fertilizer during early growing stage, but there was no difference among treatments when the plants were harvested. The total forage dry matter yields were in the range of 14,848~ 15,42kg/ha, but they were not significantly different. Also, CP, NDF, ADF and mineral contents in the forage(% of DM basis) did not differ among treatments. The pH of soil was ranged from 5.35 to 5.63, but it was not significantly different. However, the content of available $P_2O_5$ of soil was higher(P<0.05) in chemical fertilizer treatment than that of soil in fermented pig slurry treatments. The content of K was higher(p<0.05) when ffrrmented pig slurry treated with probiotics was applied after the 1st harvest. but it was not different among treatments after the and harvest.

• Asian Journal of Turfgrass Science
• /
• v.23 no.1
• /
• pp.91-100
• /
• 2009

In regional nutrient quota system, livestock manure was applied as liquid fertilizer after slurry composting and biofiltration (SCB) process. This study was conducted to evaluate the effect of SCB liquid fertilizer on turfgrass growth in golf course during 6 month period from May to October in 2008. Fertilizer treatment was designed as follows; non-fertilizer (NF), control (CF; compound fertilizer), S-1 (1L SCB${\cdot}m^{-2}$) and S-2 (2L SCB${\cdot}m^{-2}$). Every treatment was arranged in a randomized complete block design with three replications. In creeping bentgrass, turf color index, chlorophyll content, and dry weight were measured. Results were as follows; A seasonal change pattern of turf grass quality in all treatment increased in April $\sim$ June and September $\sim$ October, whereas it decreased in July $\sim$ August. As compared with NF, turf color index of CF, S-1 and S-2 increased by 1.8%, 1.8%, and 3.3%, respectively and chlorophyll content by 13%, 14%, and 20%, respectively. Dry weight of CF, S-1, and S-2 was higher than that of NFl by 7.7%, 18.2%, and 18.1%, respectively. For turf color index, chlorophyll content, and dry weigh, S-2 showed the best effect, followed by S-1 and CF in creeping bentgrass. These results indicated that the SCB application improves turf growth and quality.