• Title/Summary/Keyword: bleaching

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LHCP phosphorylation and Chlorophyll-Fluorescence Quenching of PSII in Ginseng Thylakoid Members (인삼 틸라코이드에서 광계II의 LHCP 인산화와 형광 Quenching)

  • 양덕조;김명원
    • Journal of Ginseng Research
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    • v.16 no.2
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    • pp.124-128
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    • 1992
  • Vsing the phosphorylated thylakoid membrane induced by 5-35kLux of light intensities, we investigated the chlorophyll nuorescence quenching of PSII and the phosphorylation of LHCPII i in relation to the chlorophyll-bleaching of Panax ginseng C.A. Meyer. In the Presence of DCMU, both of the fluorescence yield of non-phosphorylated thylakoid and the rate of fluorescence quencing dependent on the Phosphorylation were high p. ginseng more then Glyine max L. And at the high light F intensity (above 25 kLux) the fluorescence quenching rate of p. ginseng compared with that of G. max reached nearly to 2 times. The LHCPII of P. ginseng was composed of 3 major Polypeptides (24.5, 26 and 27kD) and 3 minor polypeptides (24, 25.3 and 28.3kD) in the region of 24-29kD and differed, from G. max in both of the number and quantity of polypeptides. Among these polypeptides, the phosphorylated polypeptide dependent on the light intensity was 24kD in P. ginseng.

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Study on the Enzyme Activity in Leaf-Burning Disease of Panax ginseng C.A. Meyer (인삼엽요병에서 효소활성도의 변화)

  • 양덕조;김명원
    • Journal of Ginseng Research
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    • v.13 no.1
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    • pp.92-97
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    • 1989
  • This study investigated the effects of high light intensity (100 KLw) and high temperature (45 ℃, dark) on enzyme (glucose-6-phosphate dehydrogenase, acid phosphatase, catalase, peroxidase, and proteinase) activities and characteristics of Panax ginseng C.A. Meyer leaves. Enzyme activity and protein content decreased rapidly under treatment with high light intensity In P ginseng the thermal stabilities of catalase and peroxidase were high (above 70%), and the coagulation rates of soluble proteins were low (below 17%). Therefore, the decrease in enzyme activity and protein content was not caused by increase in leaf temperature due to the high light intensity, but by increase in proteolytic activities. The photochemical formation rate of superoxide radical (O-2) was higher in the P ginseng leaf extracts than in Solanum nigmm, and was accelerated by addition of crude saponin to the buffer extracts.

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Mechanism of the Cucumber Cotyledon Greening Induced by KC6361 in Darkness (암조건에서 오이자엽의 녹화를 유기시키는 KC-6361 화합물의 작용기구)

  • Kim, J.S.;Kim, T.J.;Cho, K.Y.
    • Korean Journal of Weed Science
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    • v.14 no.3
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    • pp.176-183
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    • 1994
  • This study was conducted to investigate how KC6361, a new type diphenylether compound inducing bleaching, increase the greening of cucumber cotyledon in darkness. Protoporphyrin IX formation, reaccumulation rate of protochlorophyllide(Pchlide) in darkness after phototransformation and Shibata shift were not affected. Whereas, aminolevulinic acid(ALA), protochlorophyll and chlorophyll were increased, and especially protochlorophyll was significantly accumulated. When KC6361 and phytol were applied alone or in combination with ALA, the transformation from Pchlide into protochlorophyll was accelerated in darkness. These results suggest that the greening of the etiolated cucumber cotyledon treated with KC6361 seems to be caused by the accumulation of phytol or/and Geranylgeranylpyrophosphate and their increased esterification with Pchlide in darkness.

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The Effect of Potato Lipoxygenase on the Change of Lipid Distribution, Fatty Acid Composition, Carotenoids Content and Color Value in Wheat Flour Dough (감자 lipoxygenase가 밀가루반죽의 지질분포, 지방산조성, 카로테노이드 및 색도 변화에 미치는 영향)

  • Moon, Jung-Won;Suh, Myung-Ja
    • Korean Journal of Food Science and Technology
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    • v.27 no.3
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    • pp.290-293
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    • 1995
  • This study was designed to investigate the effect of potato lipoxygenase on the change of dough chemical composition including lipid distribution, fatty acid composition, carotenoids content and color value in wheat flour dough. For the study, the potato lipoxygenase was added to wheat flour at a level of $6.5{\times}10\;unit/g$ flour. The addition of potato lipoxygenase to wheat flour dough was found to cause an increase in free lipid content, an effect apparently related to the decrease in linoleic acid content and increase in peroxide value. This phenomena might be due to the enzymatic oxidation of polyunsaturated fatty acid. Also, the bleaching effect of lipoxygenase was observed as the decrease in carotenoids content of wheat flour dough. In comparison of color value, it was shown that redness, yellowness and total color difference$({\delta}E)$ were lower by addition of lipoxygenase.

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Studies on the Contents of Occuring Sulfur Dioxide in Herbal Medicines distributed at Market (유통 한약재의 이산화황 함유량에 관한 연구)

  • Sin Yeong-Min;Cho Tae Yong;Lee Kwang Soo;Kim Sung Hun;Park Heung-Jai;Leem Dong Gil;Lee Chang Hee;Kim Woo Seong;Chae Kab Ryong;Lee Young Ja;Choi Soo Young
    • Journal of Environmental Science International
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    • v.13 no.12
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    • pp.1109-1115
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    • 2004
  • This study was investigated the residual contents of sulfur dioxide on the 373 kinds of herbal medicine distributed from Korea, China, and Japan. A modified Monier-Williams method was described for the determination of $SO_{2}$ contents in herbal medicines. The residual contents of $SO_{2}$ were not detected at 221 products$(59.8{\%})$ in total 373 products. Regardless of region, $SO_{2}$ contents were not found at Farfarae Flos, Zizyphi Spinosi Semen, Castanea crenata, and Strychni Ignatii Semen. But it's found at Asparagi Radix, Codonopsitis Radix, Lilii Bulbus, and Kaempferiae Rhizoma of every region collected the samples. Also, $SO_{2}$ contents were not detected at the herbal medicines which collected cultural fields of dometic. $SO_{2}$ contents ranged 11$\~$3990 mg/kg(mean 152 mg/kg) at domestic samples, 11$\~$3440 mg/kg(mean 603 mg/kg) at imported samples. After treated with water wash and hot water extraction, the reduction rates of sulfur dioxide were appeared with $24.4{\%}\~68.7{\%}\;and\;83.8{\%}\~100.0{\%},$ respectively. These data will be used to establish a criteron of residual sulfur dioxide in herbal medicines.

Quilitative certificational plan of gegen (갈근(葛根)의 품질인증(品質認證) 방안(方案))

  • Lee, Min-Hyung;Roh, Seong-Soo;Kil, Ki-Jeong;Seo, Bu-il;Seo, Young-Bae
    • Journal of Haehwa Medicine
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    • v.13 no.2
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    • pp.179-186
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    • 2004
  • Now many sustitution and false articles is used in korea instead of gegen. To use gegen correctly, we will make a quilitative certificational plan of gegen to investigate all of lieraturea, records and documents. And we could reach conclusions as folloews. 1) Source As a source of gegen, we think all of Pueraria lobata Ohwi and Pueraria thomsonii Benth can be used in korea and china. 2) Harvesting time. According to a data, content of effective components is high when harvested in January and February. Though there is no problem because domestic harvesting and working time is between January and February, we think it need to sure well-founded datas. 3) Standars of goods According to cutting methods, gegen divide into 'jiaogegen' and 'piangegen' from users So there is no problem at cutting methods because it is not a affective factor. 4) Processing A processing method is a best important case in express quility of herb. According to a data, effective components of peeled gegen is much less than that of non-peeled geeen. A tested samples is non-peeled gegen at a researh, they received suitable judgments in all of items. Especially we got a result content of puerarin is much more than 2.0%. But they were not washed in water, it is a problem that there is bleaching effect by almost drying at a briquat. So a processing method is to wash in water with unavoidable turnning to brown, to prevent this grgrn must be dried in bulk at low temporature. And we think that content of puerarin must be looked upward.

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Water Column Correction of Airborne Hyperspectral Image for Benthic Cover Type Classification of Coastal Area (연안 해저 피복 분류를 위한 항공 초분광영상의 수심보정)

  • Shin, Jung Il;Cho, Hyung Gab;Kim, Sung Hak;Choi, Im Ho;Jung, Kyu Kui
    • Spatial Information Research
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    • v.23 no.2
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    • pp.31-38
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    • 2015
  • Remote sensing data is used to increasing efficiency on benthic cover type survey. Satellite and aerial imagery has variance of reflectance by water column effect even if bottom is consisted with same cover type and condition. This study tried to analyze advances of surveying extent and accuracy through water column correction of CASI-1500 hyperspectral image. Study area is coast of Gangneung city, South Korea where benthic environment is rapidly changing with bleaching of coral reef. Water column correction coefficient was estimated using regression models between water reflectance ($R_W$) and depth for sand bottom then the coefficients were applied to whole image. The results shows that expanded interpretable depth from 6-7m to 15m and decreased variation of reflectance by depth. Additionally, water column corrected reflectance image shows 13%p increased accuracy on benthic cover type classification.

Stabilization of Output Pulses from a Passively Q-switched Nd:YVO4 Laser Pumped by a Continuous-wave Laser Diode (연속 발진 다이오드 레이저로 여기된 수동형 Q-스위치 Nd:YVO4 레이저의 출력 펄스 안정화)

  • Ahn, Seung-In;Park, Yune-Bae;Yeo, Hwan-Seop;Lee, Joon-Ho;Lee, Kang-In;Yi, Jong-Hoon
    • Korean Journal of Optics and Photonics
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    • v.20 no.5
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    • pp.276-280
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    • 2009
  • A Cr:YAG crystal was used as a saturable absorber for passive Q-switching of a Nd:$YVO_4$ laser which was pumped by a 1-W continuous wave laser diode. The first surface of the Cr:YAG was high-reflection coated for the pump wavelength. The high-reflection coating improved the absorption efficiency of the pump beam in the Nd:$YVO_4$ through double pass absorption. It also prevented pump beam induced partial bleaching of the Cr:YAG. The peak-to-peak pulse fluctuation of passively Q-switched laser output was approximately 4 %. The minimum pulse-width was measured to be 7.11 ns. Also, the average pulse repetition rate was 9 kHz and the maximum output power was 16.27 mW.

Picosecond Dynamics of CN--Ligated Ferric Cytochrome c after Photoexcitation Using Time-resolved Vibrational Spectroscopy

  • Kim, Joo-Young;Park, Jae-Heung;Chowdhury, Salina A.;Lim, Man-Ho
    • Bulletin of the Korean Chemical Society
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    • v.31 no.12
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    • pp.3771-3776
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    • 2010
  • The dynamics of the $CN^-$-ligated ferric cytochrome c (CytcCN) in $D_2O$ at 283 K following Q-band photoexcitation at 575 nm was observed using femtosecond time-resolved vibrational spectroscopy. The equilibrium vibrational spectrum of the CN stretching mode of CytcCN shows two overlapping bands: one main band (82%) at $2122\;cm^{-1}$ with $23\;cm^{-1}$ full width at half maximum (fwhm) and the other band (18%) at $2116\;cm^{-1}$ with $7\;cm^{-1}$ fwhm. The time-resolved spectra show bleaching of the CN fundamental mode of CytcCN and two absorption features at lower energies. The bleach signal and both absorption features are all formed within the time resolution of the experiment (< 200 fs) and decay with a life time of 1.9 ps. One transient absorption feature, appearing immediately red to the bleach signal, results from the thermal excitation of low-frequency modes of the heme that anharmonically couple to the CN fundamental mode, thereby shifting the CN mode to lower energies. The shift of the CN mode decays with a lifetime of 2 ps, equivalent to the time scale for vibrational cooling of the low-frequency heme modes. The other transient absorption feature, which is 3.3 times weaker than the bleach signal and shifted $27\;cm^{-1}$ toward lower energies, is attributed to the CN mode in an electronically excited state where the CN bond is weakened with a lowered extinction coefficient. These observations suggest that photoexcited CytcCN mainly undergoes ultrafast radiationless relaxation, causing photo-deligation of $CN^-$ from CytcCN highly inefficient. As also observed in $CN^-$-ligated myoglobin, inefficient ligand photodissociation might be a general property of $CN^-$-ligated ferric hemes.

TOOTH REPLANTATION AFTER TRAUMATIC AVULSION: A 8-YEAR FOLLOW UP. (재식된 상악 중절치의 장기간에 걸친 추적례)

  • Lee, Dong-Woo;Kwak, Ji-Youn;Kim, Sung-Oh;Choi, Byung-Jai;Lee, Jae-Ho
    • Journal of the korean academy of Pediatric Dentistry
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    • v.31 no.4
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    • pp.729-733
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    • 2004
  • Tooth avulsion usually causes inflammatory root resorption and ankylosis, and ankylosis cause severe functional and esthetic problems, especially in childhood. A 7-year-old female visited the Dept. of Pediatric Dentistry, Yonsei University with the chief complaint of avulsive trauma to the upper right incisor which was left dry for 40 minutes. Tooth was irrigated with saline and replanted immediately and splinted. Anti bacterial agent and anti inflammatory agent were prescribed. After 4 months of replantation slight external root resorption and apical radiolucency was seen at radiographic examination, therfore pulp extirpation and calcium hydroxide($Vitapex^{TM}$) canal filling were carried out. After 16 months, root canal was filled with gutta-percha, and bleaching treatment was done. Treatment results were satisfactory both esthetically and functionally for 8 years and 5 months.

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