• 한국방재학회:학술대회논문집
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• 2008.02a
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• pp.279-282
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• 2008

The vertical forces in rail fasteners at areas of bridge transitions near the embankment and on the pier will occur due to different deformations of adjoining bridges caused by the trainloads. The up-lifting forces is not large problem in the blast track because the elasticity of blast and rail pad buffs up-lifting effect. But, it is likely to be difficult to ensure the serviceability of the railway and the safety of the fastener in the end in that concrete slab track consist of rail, fastener, and track in a single body, delivering directly the up-lifting force to the fastener if the deck is bended because of the end rotation of the overhang due to the vertical load. When the up-lifting force exceeds the clamp force of the fastener clip, the rail pad is out of fastener, which makes decrease the serviceability of the railway, such as noise and vibration. Furthermore, it is possible to reduce the safety of the track as the longitudinal resistance. This study is focused on guideline suggestion to decrease up-lifting force in the fastener adjacent to the civil joint of slab track of bridge throughout the parametric analysis between the vertical spring stiffness of the fastener as the material approach, the space of fastener adjacent to bridge transition, the rigidity of the girder as the geometrical approach and up-lifting force under the train load.

• Advances in concrete construction
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• v.5 no.5
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• pp.493-512
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• 2017

Blast loads may considerably affect the response of structures. In previous years, before computer analysis programs, the parameters of blast effects were calculated with empirical methods, consequently some researchers had proposed equations to find out the phenomenon. In recent year's computer analysis programs have developed already, so detailed solutions can be made numerically. This paper describes the blasting response of the structures using numerical and empirical methods. For the purpose, a reinforced concrete retaining wall is modelled using ANSYS Workbench software, and the model is imported to ANSYS AUTODYN software to perform explicit analyses. In AUTDYN software, a sum of TNT explosive is defined 5,5 m away from the wall and solution is done. Numerical results are compared with those of obtained from empirical equations. Similar study is also considered for equal explosive which is the 4 m away from the wall. The results are represented by graphics and contour diagrams of such as displacements and pressures. The results showed that distance of explosive away from the wall is highly affected the structural response of it.

• Genomics & Informatics
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• v.16 no.4
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• pp.23.1-23.5
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• 2018

Virus taxonomy was initially determined by clinical experiments based on phenotype. However, with the development of sequence analysis methods, genotype-based classification was also applied. With the development of genome sequence analysis technology, there is an increasing demand for virus taxonomy to be extended from in vivo and in vitro to in silico. In this study, we verified the consistency of the current International Committee on Taxonomy of Viruses taxonomy using an in silico approach, aiming to identify the specific sequence for each virus. We applied this approach to norovirus in Caliciviridae, which causes 90% of gastroenteritis cases worldwide. First, based on the dogma "protein structure determines its function," we hypothesized that the specific sequence can be identified by the specific structure. Firstly, we extracted the coding region (CDS). Secondly, the CDS protein sequences of each genus were annotated by the conserved domain database (CDD) search. Finally, the conserved domains of each genus in Caliciviridae are classified by RPS-BLAST with CDD. The analysis result is that Caliciviridae has sequences including RNA helicase in common. In case of Norovirus, Calicivirus coat protein C terminal and viral polyprotein N-terminal appears as a specific domain in Caliciviridae. It does not include in the other genera in Caliciviridae. If this method is utilized to detect specific conserved domains, it can be used as classification keywords based on protein functional structure. After determining the specific protein domains, the specific protein domain sequences would be converted to gene sequences. This sequences would be re-used one of viral bio-marks.

• Proceedings of the Korean Institute of Building Construction Conference
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• 2021.11a
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• pp.176-177
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• 2021

In order to compensate for the defects of concrete made using only Portland cement, three-component powder mixed with blast slag and fly ash, and four-component powder concrete mixed with silica fume are being produced. When each of the admixtures is used alone, the above-described excellent performance is expressed and up to 70% of the powder is used. These technologies are also contributing to the reduction of greenhouse gases under Act on Low Carbon. Green Growth. However, calcium hydroxide is consumed as a stimulator or reaction in the case of silica fume, which causes latent hydroponicity of slag, pozzolane reaction, and silica mixtures represented by fly ash. It is known that the consumption of calcium hydroxide affects the alkalinity of concrete. As a result, the carbonation resistance is significantly lower among the durability of concrete. Research on quantification of such effects is insufficient. In this study, an experiment was conducted to quantify calcium hydroxide of the three-component and four-component powder paste using thermal analysis equipment (DTG), and the effect of the mixing amount was discussed.

• Tunnel and Underground Space
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• v.16 no.2 s.61
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• pp.179-188
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• 2006

The vibration or/and noise generated by blast operations might cause not only structural damage to properties but mentally also to humans and animals. For that reason, maximum permitted vibration and noise levels are set by sensitivities of structures and they are used for the management of blast vibration. It is known that the fish lived in water are more sensitive to vibration than land animals, and thus the adverse impact of the blasting on fish farms should be very concerned. This study investigated the vibration and noise levels at a large eel farm located some 840 meters of the blasting site through the large real-scale experiments of blastings, prior to conducting the actual blasting. As a result, it was found that the noise met the requirement to be within maximum permitted level, while the ground vibration exceeded the permitted vibration. Accordingly, the impact of the excess vibration was investigated by an existing empirical method and verified by a new three dimensional numerical analysis. In this study, such an inspection process was briefly described, and a method was suggested for the examination of possible adverse effects from blasting on vibration-sensitive structures like the eel farm. The study also introduced a design method that controls the blast effects - ground vibration and noise.

• 한국균학회소식:학술대회논문집
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• 2015.05a
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• pp.13-13
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• 2015

Rice blast fungus, Magnaporthe oryzae, is the most destructive pathogen of rice in the world. This fungus has a biotrophic phase early in infection and switches to a necrotrophic lifestyle after host cell death. During the biotrophic phase, the fungus competes with host for nutrients and oxygen. Continuous uptake of oxygen is essential for successful establishment of blast disease of this pathogen. Here, we report transcriptional responses of the fungus to oxygen limitation. Transcriptome analysis using RNA-Seq identified 1,047 up-regulated genes in response to hypoxia. Those genes were involved in mycelial development, sterol biosynthesis, and metal ion transport based on hierarchical GO terms and well-conserved among three different fungal species. In addition, null mutants of three hypoxia-responsive genes were generated and tested for their roles on fungal development and pathogenicity. The mutants for a sterol regulatory element-binding protein gene, MoSRE1, and C4 methyl sterol oxidase gene, ERG25, exhibited increased sensitivity to hypoxia-mimetic agent, increased conidiation, and delayed invasive growth within host cells, suggesting important roles in fungal development. However, such defects did not cause any significant decrease in disease severity. The other null mutant for alcohol dehydrogenase gene, MoADH1, showed no defect in the hypoxia-mimic condition and fungal development. Taken together, this comprehensive transcriptional profiling in response to a hypoxia condition with experimental validations would provide new insights on fungal development and pathogenicity in plant pathogenic fungi.

• 한국균학회소식:학술대회논문집
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• 2015.05a
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• pp.61-61
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• 2015

Rice blast, caused by the fungal pathogen Magnaporthe oryzae, is one of the most destructive diseases of rice worldwide. The rice - M. oryzae pathosystem has become a model in the study of plant - fungal interactions due to its economic importance and accumulating knowledge. During the evolutionary arms race with M. oryzae, rice plants evolved a repertoire of Resistance (R) genes to protect themselves from diseases in a gene-for-gene fashion. M. oryzae secretes a battery of small effector proteins to manipulate host functions for its successful infection, and some of them are recognized by host R proteins as avirulence effectors (AVR), which turns on strong immunity. Therefore, the analysis of interactions between AVRs and their cognate R proteins provide crucial insights into the molecular basis of plant - fungal interactions. Rice blast resistance genes Pik, Pia, Pii comprise pairs of protein-coding ORFs, Pik-1 and Pik-2, RGA4 and RGA5, Pii-1 and Pii-2, respectively. In all three cases, the paired genes are tightly linked and oriented to the opposite directions. In the AVR-Pik/Pik interaction, it has been unraveled that AVR-Pik binds to the N-terminal coiled-coil domain of Pik-1. RGA4 and RGA5 are necessary and sufficient to mediate Pia resistance and recognize the M. oryzae effectors AVR-Pia and AVR1-CO39. A domain at the C-terminus of RGA5 characterized by a heavy metal associated domain was identified as the AVR-binding domain of RGA5. Similarly, physical interactions among Pii-1, Pii-2 and AVR-Pii are being analyzed.

• The Korean Journal of Malacology
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• v.31 no.3
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• pp.243-247
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• 2015

A stand-alone BLAST server is available that provides a convenient and amenable platform for the analysis of molluscan sequence information especially the EST sequences generated by traditional sequencing methods. However, it is found that the server has limitations in the annotation of molluscan sequences generated using next-generation sequencing (NGS) platforms due to inconsistencies in molluscan sequence available at NCBI. We constructed a web-based interface for a new stand-alone BLAST, called PANM-DB (Protostome DB) for the analysis of molluscan NGS data. The PANM-DB includes the amino acid sequences from the protostome groups-Arthropoda, Nematoda, and Mollusca downloaded from GenBank with the NCBI taxonomy Browser. The sequences were translated into multi-FASTA format and stored in the database by using the formatdb program at NCBI. PANM-DB contains 6% of NCBInr database sequences (as of 24-06-2015), and for an input of 10,000 RNA-seq sequences the processing speed was 15 times faster by using PANM-DB when compared with NCBInr DB. It was also noted that PANM-DB show two times more significant hits with diverse annotation profiles as compared with Mollusks DB. Hence, the construction of PANM-DB is a significant step in the annotation of molluscan sequence information obtained from NGS platforms. The PANM-DB is freely downloadable from the web-based interface (Malacological Society of Korea, http://malacol.or/kr/blast) as compressed file system and can run on any compatible operating system.

• The Plant Pathology Journal
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• v.36 no.4
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• pp.314-322
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• 2020

Interplay between histone acetylation and deacetylation is one of the key components in epigenetic regulation of transcription. Here we report the requirement of MoHDA1-mediated histone deacetylation during asexual development and pathogenesis for the rice blast fungus, Magnaporthe oryzae. Structural similarity and phylogenetic analysis suggested that MoHDA1 is an ortholog of Saccharomyces cerevisiae Hda1, which is a representative member of class II histone deacetylases. Targeted deletion of MoHDA1 caused a little decrease in radial growth and large reduction in asexual sporulation. Comparison of acetylation levels for H3K9 and H3K14 showed that lack of MoHDA1 gene led to significant increase in H3K9 and H3K14 acetylation level, compared to the wild-type and complementation strain, confirming that it is a bona fide histone deacetylase. Expression analysis on some of the key genes involved in asexual reproduction under sporulation-promoting condition showed almost no differences among strains, except for MoCON6 gene, which was up-regulated more than 6-fold in the mutant than wild-type. Although the deletion mutant displayed little defects in germination and subsequent appressorium formation, the mutant was compromised in its ability to cause disease. Wound-inoculation showed that the mutant is impaired in invasive growth as well. We found that the mutant was defective in appressorium-mediated penetration of host, but did not lose the ability to grow on the media containing H₂O₂. Taken together, our data suggest that MoHDA1-dependent histone deacetylation is important for efficient asexual development and infection of host plants in M. oryzae.

• Journal of the Korean Society of Hazard Mitigation
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• v.9 no.5
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• pp.69-78
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• 2009

Since the blast vibration induced by explosives of the powder possibly provide damage of the nearby structures adjacent to the tunnel, the stability of the nearby structures should be estimated. In this study, the stability of the tunnel based on the allowable peak particle velocity of the structures as well as allowable stress of the structures presented in the concrete structural design standard was estimated with respect to the stress of the concrete lining and axial force of the rockbolt during the blasting operation at the ground surface of the pre-existing tunnel. The analyses were carried out by using $FLAC^{2D}$ which is one of the programs developed based on the finite difference method. The bending compressive stress and shear stress of the concrete lining and axial force of the rockbolt were rapidly increased when the blasting operation was conducted near the tunnel.