• Journal of Animal Science and Technology
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• 제62권2호
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• pp.187-197
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• 2020

The present study was conducted to evaluate the effects of black soldier fly larvae oil (BSFLO) from the black soldier fly larvae (BSFL) as a partial or total replacement of soybean oil (SBO) on growth performance, fatty acid (FA) profile, and meat quality of broiler chickens from 1 to 5 wk of age. A total of 210 male broiler chickens (Ross 308) at one-day of age were randomly allotted to 3 dietary treatments (10 replicates and 7 birds/group): a basal control diet (CON), the basal diet in which the SBO was replaced by 50% (50 BSFLO) or 100% (100 BSFLO) of BSFLO. The growth performance, physical measurements and chemical traits of leg meat, and sensory analysis of breast meat were not influenced by diets. However, the relative weight (g/kg) of gizzard of CON was significantly higher (14.85, 12.52, and 13.02 for CON, 50 BSFLO, and 100 BSFLO; p < 0.05) than that of other treatments. As expected, the FA profile of breast meat was affected by BSFLO inclusion. The proportion (%) of saturated fatty acid (SFA) was increased (27.16, 27.58, and 28.72 for CON, 50 BSFLO, and 100 BSFLO; p < 0.05) by BSFLO inclusion and the percentage (%) of MUFA was also increased (43.36, 44.58, and 48.55 for CON, 50 BSFLO, and 100 BSFLO; p < 0.01). On the contrary, the proportion (%) of PUFA was decreased (29.49, 27.84, and 22.74 for CON, 50 BSFLO, and 100 BSFLO; p < 0.01). In conclusion, the present study suggests that the replacement of BSFLO did not show an adverse effect on growth performance and it could be an ingredient as a dietary fat source for a broiler diet.

• 농업과학연구
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• 제47권2호
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• pp.219-227
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• 2020

Among insect species, black soldier fly larvae (BSFL) is a promising ingredient for animal feed as a dietary source. Moreover, BSFL contains a high content of lauric acid (C12:0), which has antimicrobial effects. Therefore, this study evaluated the effect of BSFL oil (BSFLO) as a partial or total replacement of soybean oil (SBO) on the cecal microbiota in broilers. A total of 210 male broiler chickens (Ross 308) at one-day of age were randomly allotted to 3 dietary treatments (10 replicates and 7 birds/group): a basal control diet (CON), the basal diet in which SBO was replaced by 50% (50 BSFLO) or 100% (100 BSFLO) BSFLO. At the end of the study (d 35), 18 birds (6 broilers/treatment) were randomly selected and slaughtered. Samples of cecal digesta were collected to verify their cecal microbiota. Overall, 235,978 gene sequences were generated, and a total of 4,398 operational taxonomic units were identified in the three groups. At the phylum level, Firmicutes was the dominant phyla in all three groups. At the genus level, Faecalibacterium was the dominant genera in all the treatments. There were no significant differences in the relative abundances of all the genera between the BSFLO groups and CON. However, the genus Erysipelatoclostridium was more abundant in the 50 BSFLO group than in the CON (p < 0.05). In conclusion, the substitution of SBO with BSFLO in broiler diets had no negative effect on the cecal microbiota of broilers.

• Animal Bioscience
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• 제35권9호
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• pp.1408-1417
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• 2022

Objective: The objective of this study was to determine whether dietary black soldier fly (Hermetia illucens, HI) larvae oil (HILO) could serve as an alternative fat source to soybean oil (SBO) in laying hen diets. Methods: We randomly assigned 25-week-old Hy-line Brown laying hens (n = 144) to receive (n = 6 hens/group; eight replicates) a control or an experimental diet in which SBO was replaced with 50% (50HILO) or 100% HILO (100HILO). Results: Dietary HILO did not negatively affect body weight or productive performance during the study. The eggs also had similar quality parameters, proximate composition, and cholesterol levels. However, the yolk color index was significantly higher (p<0.01) in the 100HILO than in the other groups. Dietary HILO significantly altered the composition of fatty acids (FAs) in abdominal fat and eggs. Total saturated fatty acids (SFAs) and total polyunsaturated FAs (PUFAs) were significantly increased and decreased in the 50HILO and 100HILO groups, respectively, compared with those in the control group (p<0.001 and p<0.0001, respectively). Specifically, the medium-chain FAs lauric and myristic acids were remarkably increased in the abdominal fat of laying hens fed HILO (p<0.0001), whereas only myristic acid increased in eggs (p<0.0001). Undesirable heavy metal (aluminum, fluorine, arsenic, lead, mercury, and cadmium) concentrations were below permissible limits in eggs. Conclusion: We considered that HILO could be an alternative dietary fat to SBO for laying hens with maintained productive performance and good egg quality.

• 한국수산과학회지
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• 제55권5호
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• pp.533-540
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• 2022

We evaluated a worm-based extruded pellet diet with black soldier fly Hermetia illucens larvae (BSF) meal and BSF oil for olive flounder Paralichthys olivaceus through field feeding experiments at a commercial aquafarm. We prepared two experimental diets by replacing fish meal and fish oil with BSF meal and BSF oil (BEP-1, BSF meal 7%, BSF oil 1%) and (BEP-2, BSF oil 2%), respectively. We prepared raw-fish based moist pellets (MP) for comparison between the two experimental diets. We distributed the olive flounder (220±6.29 g) in square (10 m×10 m×1 m) concrete, 100 ton tanks at a density of 1,600 fish per tank (n=3) in triplicate for each dietary treatment. We fed the diets to the fish to apparent satiation for 7 months. At the end of the feeding trial, we found no substantial differences between the groups in terms of growth performance, survival, or feed utilization. None of the diet groups showed any changes in either hematological or non-specific immune responses. The histological observation of the intestine showed that the goblet cell number and cholecystokinin-producing cell activity increased in the fish fed the BEP diet compared with the those of the fish fed the MP diet. These results indicated that dietary BSF meal and oil can be used for olive flounder without compromising growth or, hematological and histological parameters.

• 생명과학회지
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• 제31권12호
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• pp.1094-1099
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• 2021

동애등에(Hermetia illucens)에 대한 몇 가지 약리적 효능이 보고된 바 있으나, 이 추출물의 항비만 및 작용기전에 대한 보고는 전무한 실정이다. 본 연구에서는 3T3-L1 지방전구세포를 지방세포로 분화시키는 과정에 동애등에 유충 에탄올 추출물을 첨가하여 분화 억제를 통한 항비만 효과를 확인하고자 하였다. 지방세포 분화에서 나타나는 중성지방의 양을 Oil red O 염색을 통하여 지방 축적 정도를 측정하였고, 지방세포의 분화와 지방 합성에 관련된 인자들의 발현량을 western blot을 통해 확인하였다. 동애등에 추출물에 의해 지방세포의 지방구 형성은 유의한 수준으로 억제되었다. 또한, 지방세포의 분화에 관여하는 전사인자인 peroxisome proliferator-activated receptor γ (PPARγ)와 CCAAT enhancer-binding protein α (C/EBPα)의 단백질 발현은 400 ㎍/ml 동애등에 추출물에 의해 현저하게 감소되었다. 이에 본 연구결과를 토대로 동애등에 추출물은 지방세포 분화 및 지방 축적 억제를 통한 항비만 소재로 활용하기에 적합할 것으로 판단된다.