Korean Journal of Agricultural Science (농업과학연구)

Institute of Agricultural Science, CNU (충남대학교 농업과학연구소)
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Effects of black soldier fly larvae (Hermetia illucens) oil on cecal microbiota in broilers

  • Kim, Byeonghyeon (National Institute of Animal Science, Rural Development Administration) ;
  • Bang, Han Tae (National Institute of Animal Science, Rural Development Administration) ;
  • Jeong, Jin Young (National Institute of Animal Science, Rural Development Administration) ;
  • Kim, Min Ji (National Institute of Animal Science, Rural Development Administration) ;
  • Kim, Ki Hyun (National Institute of Animal Science, Rural Development Administration) ;
  • Chun, Ju Lan (National Institute of Animal Science, Rural Development Administration) ;
  • Reddy, Kondreddy Eswar (National Institute of Animal Science, Rural Development Administration) ;
  • Ji, Sang Yun (National Institute of Animal Science, Rural Development Administration)
  • Received : 2020.03.16
  • Accepted : 2020.04.28
  • Published : 2020.06.01
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Abstract

Among insect species, black soldier fly larvae (BSFL) is a promising ingredient for animal feed as a dietary source. Moreover, BSFL contains a high content of lauric acid (C12:0), which has antimicrobial effects. Therefore, this study evaluated the effect of BSFL oil (BSFLO) as a partial or total replacement of soybean oil (SBO) on the cecal microbiota in broilers. A total of 210 male broiler chickens (Ross 308) at one-day of age were randomly allotted to 3 dietary treatments (10 replicates and 7 birds/group): a basal control diet (CON), the basal diet in which SBO was replaced by 50% (50 BSFLO) or 100% (100 BSFLO) BSFLO. At the end of the study (d 35), 18 birds (6 broilers/treatment) were randomly selected and slaughtered. Samples of cecal digesta were collected to verify their cecal microbiota. Overall, 235,978 gene sequences were generated, and a total of 4,398 operational taxonomic units were identified in the three groups. At the phylum level, Firmicutes was the dominant phyla in all three groups. At the genus level, Faecalibacterium was the dominant genera in all the treatments. There were no significant differences in the relative abundances of all the genera between the BSFLO groups and CON. However, the genus Erysipelatoclostridium was more abundant in the 50 BSFLO group than in the CON (p < 0.05). In conclusion, the substitution of SBO with BSFLO in broiler diets had no negative effect on the cecal microbiota of broilers.

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References

  1. Belghit I, Waagbo R, Lock EJ, Liland NS. 2019. Insect-based diets high in lauric acid reduce liver lipids in freshwater Atlantic salmon. Aquaculture Nutrition 25:343-357. https://doi.org/10.1111/anu.12860
  2. Cullere M, Schiavone A, Dabbou S, Gasco L, Dalle Zotte A. 2019. Meat quality and sensory traits of finisher broiler chickens fed with black soldier fly (Hermetia illucens L.) larvae fat as alternative fat source. Animals 9:1-15.
  3. Gangadoo S, Dinev I, Chapman J, Hughes RJ, Van TTH, Moore RJ, Stanley D. 2018. Selenium nanoparticles in poultry feed modify gut microbiota and increase abundance of Faecalibacterium prausnitzii. Applied Microbiology and Biotechnology 102:1455-1466. https://doi.org/10.1007/s00253-017-8688-4
  4. Hermanns D, Martel A, Van Deun K, Verlinden M, Van Immersel F, Garmyn A, Messens W, Heyndrickx M, Haesebrouck F, Pasmans F. 2010. Intestinal mucus protects Campylobacter jejuni in the ceca of colonized broiler chickens against the bactericidal effects of medium-chain fatty acid. Poultry Science 89:1144-1155. https://doi.org/10.3382/ps.2010-00717
  5. Kers JG, Velkers FC, Fischer EAJ, Hermes GDA, Lamot DM, Stegeman JA, Smidt H. 2019. Take care of the environment: Housing conditions affect the interplay of nutritional interventions and intestinal microbiota in broiler chickens. Animal Microbiome 1:1-14. https://doi.org/10.1186/s42523-019-0003-5
  6. Kim JK, Lei XJ, Lee SI, Lee IS, Kim IH. 2017. Evaluation of rapeseed meal as a protein source to replace soybean meal in growing pigs. Korean Journal of Agricultural Science 44:235-243. https://doi.org/10.7744/kjoas.20170023
  7. Kim S, Kim B, Kim Y, Jung S, Kim Y, Park J, Song M, Oh S. 2016. Value of palm kernel co-products in swine diets. Korean Journal of Agricultural Science 43:761-768. https://doi.org/10.7744/kjoas.20160079
  8. Liu C, Finegold SM, Song Y, Lawson PA. 2008. Reclassification of Clostridium coccoides, Ruminococcus hansenii, Ruminococcus hydrogenotrophicus, Ruminococcus luti, Ruminococcus productus and Ruminococcus schinkii as Blautia coccoides gen. nov., comb. nov., Blautia hansenii comb. nov., Blautia hydrogenotrophica comb. nov., Blautia luti comb. nov., Blautia producta comb. nov., Blautia schinkii comb. nov. and description of Blautia wexlerae sp. nov., isolated from human faeces. International Journal of Systematic and Evolutionary Microbiology 58:1896-1902. https://doi.org/10.1099/ijs.0.65208-0
  9. Li Z, Wang W, Liu D, Guo Y. 2017. Effects of Lactobacillus acidophilus on gut microbiota composition in broilers challenged with Clostridium perfringens. PLoS One 12:e0188634. https://doi.org/10.1371/journal.pone.0188634
  10. Lock ER, Arsiwalla T, Waagbo R. 2016. Insect larvae meal as an alternative source of nutrients in the diet of Atlantic salmon (Salmo salar) postsmolt. Aquaculture Nutrition 22:1202-1213. https://doi.org/10.1111/anu.12343
  11. Makkar HPS, Tran G, Heuze V, Ankers P. 2014. State-of-the-art on use of insects as animal feed. Animal Feed Science and Technology 197:1-33. https://doi.org/10.1016/j.anifeedsci.2014.07.008
  12. Miquel S, Martin R, Rossi O, Bermudez-Humaran LG, Chatel JM, Sokol H, Thomas M, Wells JM, Langella P. 2013. Faecalibacterium prausnitzii and human intestinal health. Current Opinion in Microbiology 16:255-261. https://doi.org/10.1016/j.mib.2013.06.003
  13. Newton GL, Sheppard DC, Watson DW, Burtle GJ, Dove CR. 2005. Using the black soldier fly, Hermetia illucens, as a value-added tool for the management of swine manure. Animal and Poultry Waste Management Center, North Carolina State University, Raleigh, NC, USA.
  14. Schiavone A, Cullere M, De Marco M, Meneguz M, Biasato I, Bergagna S, Dezzutto D, Gai F, Dabbou S, Gasco L, Dalle Zotte A. 2017. Partial or total replacement of soybean oil by black soldier fly larvae (Hermetia illucens L.) fat in broiler diets: Effect on growth performances, feed-choice, blood traits, carcass characteristics and meat quality. Italian Journal of Animal Science 16:93-100. https://doi.org/10.1080/1828051X.2016.1249968
  15. Schiavone A, Dabbou S, De Marco M, Cullere M, Biasato I, Biasibetti E, Capucchio MT, Bergagna S, Dezzutto D, Menequz M, Gai F, Dalle Zotte A, Gasco L. 2018. Black soldier fly larva fat inclusion in finisher broiler chicken diet as an alternative fat source. Animal 12:2032-2039. https://doi.org/10.1017/S1751731117003743
  16. Sheppard DC, Newton GL, Thompson SA, Savage S. 1994. A value added manure management system using the black soldier fly. Bioresource Technology 50:275-279. https://doi.org/10.1016/0960-8524(94)90102-3
  17. Skrivanova E, Marounek M, Dlouha G, Kanka J. 2005. Susceptibility of clostridium perfringens to C-C fatty acids. Letters in Applied Microbiology 41:77-81. https://doi.org/10.1111/j.1472-765X.2005.01709.x
  18. Spranghers T, Michiels J, Vrancx J, Ovyn A, Eeckhout M, De Clercq P, De Smet S. 2018. Gut antimicrobial effects and nutritional value of black soldier fly (Hermetia illucens L.) prepupae for weaned piglets. Animal Feed Science and Technology 235:33-42. https://doi.org/10.1016/j.anifeedsci.2017.08.012
  19. Surendra KC, Olivier R, Tomberlin JK, Jha R, Khanal SK. 2016. Bioconversion of organic wastes into biodiesel and animal feed via insect farming. Renewable Energy 98:197-202. https://doi.org/10.1016/j.renene.2016.03.022
  20. Suzuki T. 2013. Regulation of intestinal epithelial permeability by tight junctions. Cellular and Molecular Life Sciences 70:631-659. https://doi.org/10.1007/s00018-012-1070-x
  21. Wise TA. 2013. Can we feed the world in 2050? A scoping paper to assess the evidence. Tufts University, Medford, OR, USA.
  22. Zeitz JO, Fennhoff J, Kluge H, Stangl GI, Eder K. 2015. Effects of dietary fats rich in lauric and myristic acid on performance, intestinal morphology, gut microbes, and meat quality in broilers. Poultry Science 94:2404-2413. https://doi.org/10.3382/ps/pev191
  23. Zentek J, Buchheit-Renko S, Manner K, Pieper R, Vahjen W. 2012. Intestinal concentrations of free and encapsulated dietary medium-chain fatty acids and effects on gastric microbial ecology and bacterial metabolic products in the digestive tract of piglets. Archives of Animal Nutrition 66:14-26. https://doi.org/10.1080/1745039X.2011.644916