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Ethanol Extract of Hermetia illucens Larvae Inhibits Adipogenesis in 3T3-L1 Adipocytes (동애등에 유충 에탄올 추출물의 지방세포 분화 억제 효과)

  • Park, Ji Yeong;Kwak, Kyu-Won;Choi, Ji-Yeon;Lee, Si-Eun;Kim, Yong-Soon;Koo, Bonwoo;Kim, Eunsun;Park, Kwanho;Kim, Sun Young
    • Journal of Life Science
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    • v.31 no.12
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    • pp.1094-1099
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    • 2021
  • Hermetia illucens (Black soldier fly) is attracting attention as an environmental purification insect because it can supply a wide range of by-products of the agricultural food industry. Also, it has a potential feed for fish, birds, and pets due to a short life cycle and excellent nutritional components. Several pharmacological effects, including antimicrobial, of H. illucens have been reported. However, no study has focused on antiobesity effects of ethanol extract of H. illucens. In this study, we aimed to assess the anti-obesity effects of ethanol extract of H. illucens larvae (HIE) through inhibition of differentiation of 3T3-L1 preadipocytes into adipocytes. The amount of lipid accumulated in adipocytes was measured by oil red-O staining, and the inhibitory effect on adipogenesis was confirmed. The expression levels of factors related to adipocyte differentiation and fat synthesis were determined using Western blot analysis. Lipid droplet formation in adipocytes was remarkably inhibited by HIE. In addition, treatment with 400 ㎍/ml of HIE significantly reduced the expression levels of peroxisome proliferator-activated receptor γ and CCAAT/enhancer-binding protein α-transcription factors involved in adipocyte differentiation. Therefore, the results of this study indicate that HIE is a potential anti-obesity agent because it inhibits adipocyte differentiation.

Comparison of Total and Inorganic Arsenic Contamination in Grain and Processed Grain Foods (곡류 및 곡류 가공식품의 총비소 및 무기비소 오염 비교)

  • Eun-Jin, Baek;Myung-Gil, Kim;Hyun-Jue, Kim;Jin-Hee, Sung;You-Jin, Lee;Shin-Hye, Kwak;Eun-Bin, Lee;Hye-Jin, Kim;Won-Joo, Lee;Myung-Jin, Lee
    • Journal of Food Hygiene and Safety
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    • v.37 no.6
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    • pp.385-393
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    • 2022
  • The contamination level of inorganic arsenic, a human carcinogen, was investigated in 87 grains and 66 processed grain foods. Two inorganic arsenic species arsenite (As(III)) and arsenate (As(V)) and four organic arsenic monomethylarsonic acid, dimethylarsinic acid, arsenobetaine, arsenocholine were analyzed using HPLC-ICP/MS with high separation and sensitivity and ICP/MS was used to quantify total arsenic. Inorganic arsenic was detected in all grains. And the total arsenic in grains consists of about 70-85% inorganic arsenic and about 10-20% DMA. The concentration of inorganic arsenic was high in rice and black rice cultivated in paddy soil with irrigated water, while the miscellaneous grain in field was low. Mean concentration of inorganic arsenic in rice germ, brown rice and polished rice was 0.160 mg/kg, 0.135 mg/kg, 0.083 mg/kg, respectively, indicating that rice bran contains more arsenic. In processed grain foods, inorganic arsenic concentration varied according to the kind of ingredients and content, and the detection amount was high in processed food with brown rice and germ. The arsenic content of all samples did not exceed each standard, but the intake frequency is high and it is considered that continuous monitoring is necessary for food safety.

Scientific Analysis and Conservation Treatment on the Buddhist Scriptures of Paper Relics Excavated from Sum Tolgoi, Mongolia (몽골 숨 톨고이 출토 지류 유물의 과학적 분석 및 보존처리)

  • Bae, Su Bin;Yang, Min Jeong;Kwon, Yun Mi;Yoo, Ji Hyun;Jeong, Hee Won
    • Journal of Conservation Science
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    • v.37 no.6
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    • pp.723-737
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    • 2021
  • This study analyzed the composition and structure of materials with Buddhist paper scriptures excavated from architectural sites in 'Sum Tolgoi' of the 17th century and carried out conservation treatment base on the result of the analysis. The scriptures were covered in dust and foreign sub stances, and were so crumpled that it was impossible to identify the form. The damage, loss, and discoloration have been identified. Buddhist scriptures written in Tibetan used indigo and ink sticks on paper as a result of UV-Vis analysis, and ink sticks as black character materials from scriptures written ancient Mongolian. SEM-EDS and Micro-XRF analyses revealed that the outlines were drawn with red lines using a mix of Minium (Pb3O4) and Cinnabar (HgS), or Cinnabar (HgS) alone, and the contents of the scriptures were written with silver paint. Silver chloride (AgCl) and Calcium (Ca) were identified in the silver paint component of the characters, while Calcium and Orpiment (As2S3) were identified in the yellow lines. Concerning the paper ground, Buddhist scriptures written in ancient Mongolian were characterized by herbal plant fiber and bast fiber, and those written in Tibetan, by bast fiber. Radiocarbon dating indicates that the paper for the scriptures was produced between the 15th and 17th centuries. Conservation treatment of the scriptures was carried out based on the experiment on the production of pre-coated paper and how to coat that to prevent the second damage due to the deformation and fragility of the excavated paper. The scriptures were preserved and mounted, and a neutral box was made to identify the contents of the scriptures recorded on both sides after the treatment. This conservation treatment is the result of a study that applied new conservation treatment materials and methods according to the principle of conservation treatment reversibility.

A Preliminary Study on the Post-magmatic Activities Occurring at the Gonamsan Gabbroic Rocks in the Pocheon Area (포천지역 고남산 반려암질암 내 발생하는 후기 화성활동에 관한 예비 연구)

  • Lee, Ji-Hyun;Kim, Eui-Jun;Shin, Dongbok
    • Economic and Environmental Geology
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    • v.55 no.1
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    • pp.77-95
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    • 2022
  • The Gonamsan gabbroic complex in the Pocheon area, northwestern region of South Korea consists of a variety types of gabbroic rocks and associated Fe-Ti oxide deposits caused by magmatic differentiation. Post-magmatic intrusions (i.e., gabbroic pegmatite and pyroxene-apatite-zircon rocks) partly intruded into the gabbroic rocks. The gabbroic pegmatite occurs in monzodiorite and oxide gabbro of the complex, intimately and spatially associated with high-grade lenticular Fe-Ti oxide mineralization. The pegmatite can be subdivided into plagioclase-amphibole and pyroxene-olivine pegmatite, in which the contact surface is sharp. The plagioclase-amphibole pegmatite comprises plagioclase and amphibole, with lesser amount of pyroxene, ilmenite, sphene, apatite, and biotite. The pegmatite shows plagioclase-amphibole intergranular texture, in which the open space formed by large plagioclase laths (An2-26Ab72-98Or0-2) are infilled by amphibole. The pyroxene-olivine pegmatite is dark gray to black in color and also contains magnetite, ilmenite, spinel, apatite, and calcite as a minor component. The pyroxene (En35-36Fs8-9Wo55) and olivine (Fo84-85Fa15-16) partly show a poikilitic texture defined by smaller euhedral olivine enclosed by coarser clinopyroxene. Fe-Ti oxide minerals consist mainly of magnetite and ilmenite that are found interstitially to earlier formed silicates. Subsequently, they are encompassed by reaction rim (almost of amphibole and biotite) along the boundary with surrounding silicate minerals. Under the microscope, magnetite contains a lot of oxyexsolved ilmenite (trellis type) and spinel, and thereby is weakly enriched in magnetite-compatible elements such as Ti, Al, Mg, and V. The structure and textures at the contact zone as well as mineralogical disequilibrium between gabbroic pegmatite and the host gabbroic rocks suggest that the pegmatite may form as a result of accumulation from Fe-rich melt (or liquid) that occurred somewhere rather than in situ form from the host gabbroic rock during the magmatic differentiation. Consequently, the preliminary study suggests that further study on the post-magmatic activities can not only help us improve our understanding on magmatic fractionation but also provide critical information on Fe-Ti oxide mineralization in gabbroic rocks resulting from the magmatic differentiation.

Ecological Characteristics of Korean Dark Sleeper, Odontobutis interrupta in Geumdang Stream, Korea (금당천에 서식하는 얼록동사리(Odontobutis interrupta)의 생태 특징)

  • Hwa-Keun Byeon
    • Korean Journal of Environment and Ecology
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    • v.37 no.1
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    • pp.86-93
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    • 2023
  • This study investigated the ecological characteristics of Odontobutis interrupta at the Geumdang Stream from January to December 2021. The riverbed structure of the species habitat was rich in sand and mud. The water was deep, ranging from 21 to 124 cm, with an average of 48 cm. The stream velocity was slow at 0.24 (0.08-0.36) m/sec. The ratio of females to males was 1:0.98, and the total length of collected individuals ranged from 23 mm to 162 mm. The age according to the total length-frequency distribution as of May indicated that the group with a total length of 23-59 mm was one year old, the group with 60-99 mm was two years old, the group with 100-139 mm was three years old, and the group with 140-162 mm was four years old. As a secondary gender characteristic, the females genital papilla had a cylindrical shape, a hollow inside of the tip, and a longer diameter than males. The males had a cone shape with a pointed end. Sexually mature males had the nuptial color, with a black abdomen and whole body. Some females with a length ranging from 60 to 69 mm and all females 70 mm longer were sexually mature. Some males with a length ranging from 70 to 79 mm and all males 80 mm longer were sexually mature. The spawning season was from May to July, and the water temperature was between 19.6℃ to 29℃ during that period. The prosperous spawning season was June (26℃). The average number of eggs in the ovaries was 2,473 (883-4,955) per matured female, and the matured eggs were yellowish and spherical with a mean diameter of 1.42 (1.20-0.54) mm. The correlation between total length and weight was BW=0.0000006TL3.21 with the constant a as 0.0000006 and parameter b as 3.21. The condition factor (K) was 1.67 (1.18-2.43) on average, and the slope was 0.116.

A Study on Heart-Mind of Daesoon Thought from the Perspective of Neo-Confucianism: Focused on Numinous Emptiness, Wise-awakening, and Divine Beings (주자학으로 본 대순사상의 마음에 관한 연구 - 허령, 지각, 신명을 중심으로 -)

  • Choi, Chi-bong
    • Journal of the Daesoon Academy of Sciences
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    • v.31
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    • pp.237-269
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    • 2018
  • This study aims to look into Daesoon Thought from the perspective of Neo-Confucianism. Numinous emptiness (虛靈), wise-awakening (知覺), and divine beings (神明) in the Scripture of the Black Tortoise (Hyeonmu-gyeong) are often mentioned as characteristics of the mind in Neo-Confucianism. Accordingly, this research take up the 'Che-Yong (substance, 體 and function, 用)' of mind by classifying numinous emptiness as Origin-Substance (本體), wise-awaking as function, and divine beings as subjects. Numinous emptiness enables an individual to have been born with Li (理) of Taegeuk (太極), which is a mandate from Heaven and the nature of humanity; whereas one embodies spirit and brightness mind based on Origin-Substance. This numinous emptiness of mind is precisely the mind-spirit. Wise-awakening is a function of mind, which actually awakens numinous emptiness (理) and objects (氣). The mind which realizes the Li of Taegeuk as numinous emptiness is conscientiousness and can be taken as a Dao-Mind. The mind which desires objects refers to the Human Mind or a selfish motive. Such propensity in terms of wise-awakening determines the state of mind. One should reach the state of a quiet mind by wisely awakening through the scripture. Divine beings correspond to metaphysical Origin-Substance and physical objects respectively. In addition, they comprehend all and preside over wise-awakening as subjects. The subject recognizes wise-awakening and responds to it. Mind is a path traveled upon by divine beings as they enter and exit. In this regard, the human mind refers to the organ used by immanent deities, which accumulates physical senses, innermost mental processes, and awakened activities. Furthermore, Transcendental deities also come in and out through mental correspondence, leading to changes in one's physical constitution or personality. This paper focuses on the mind perceived in Daesoon Thought as follows: first, besides the existing spiritual perspective, the research takes a view on surveying numinous emptiness, wise-awakening, and divine beings in Neo-Confucianism. Secondly, the Che-Yong of mind is closely looked into by means of innate numinous emptiness as Che and wise-awakening as Yong. Lastly, the essence, energy, and divinity as well as spiritual soul and physical soul are mentioned according to the concept of an immanent deity. The paper also clarifies the fact that divine beings preside over the mind as subjects.

Daesoon Jinrihoe Yeoju Headquarters Temple Complex as Viewed within Feng-Shui Theory (풍수지리로 본 대순진리회 여주본부도장)

  • Shin, Young-dae
    • Journal of the Daesoon Academy of Sciences
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    • v.33
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    • pp.91-145
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    • 2019
  • This study aims to reveal that Daesoon Jinrihoe Yeoju Headquarters Temple Complex is a sacred place of Gaebyeokgongsa (the Reordering Works of the Great Opening) through the logic of the energy of form in Feng-Shui studies. The Headquarters Temple Complex can illuminate the lamp of coexistence, emerge as a place for cultivation, and support the era of human nobility with Gucheonsangje (the Supreme God of the Ninth Heaven) as an object of faith. Virtuous Concordance of Yin and Yang, Harmonious Union between Divine Beings and Human Beings, the Resolution of Grievances for Mutual Beneficence, and Perfected Unification with Dao are the mission statements of this great site. For this purpose, it is necessary to investigate the headquarters according to integral Feng-Shui Theory. Doing so can provide proof that the geographic location, landscape, yin-yang harmonizing, and flowing veins of terrestrial energy at Headquarters Temple Complex are all profoundly auspicious. At the same time, this data also allows further study into the interactions of dragon-veins, energy hubs, surrounding mountains, and watercourses, which reveal how Daesoon Jinrihoe Yeoju Headquarters Temple Complex promotes the basic works of propagation, edification, and cultivation and three societal works of charity aid, social welfare, and education for the purpose of global propagation, saving beings, and building an earthly paradise by reforming humanity and engaging in spiritual civilization. This must be done on site with proper Feng-Shui in order to open up the era of human nobility upon the Great Opening of the Later World. As the center of the religious order, Daesoon Jinrihoe, Yeoju Headquarter Temple Complex has the general Feng-Shui characteristic of Baesanimsu (a back supported by a mountain and a front facing water). Through discussing the Feng-Shui of Daesoon Jinrihoe's Yeoju Headquarters Temple Complex as the center of humankind's resolution of grievances for mutual beneficence, this study would explore growth-supporting land that delivers future rewards through Feng-Shui symbolism and the ethical practice of grateful reciprocation of favors for mutual beneficence. This exploration will reveal how the geographical features and conditions of the Yeoju Headquarters Temple Complex make it a place fit for spiritual cultivation. It is a miraculous luminous court surrounded by mountains, where auspicious signs in eight directions gather. Its veins of terrestrial energy harmonize with clean water energy as it is affectionately situated within its natural environment. Its location corresponds with the Feng-Shui theory of dragon-veins, energy hubs, surrounding mountains, and watercourses. Thus, with regards to the Feng-Shui of Daesoon Jinrihoe's Yeoju Headquarters Temple Complex, this study examines the flows of mountains and waters and focuses on how the site is based on the logic of Feng-Shui. More generally, the geographical features of the surrounding mountains are likewise examined. An analysis of the relationship between Poguk (布局) of Sasinsa (animal symbols of the four directions, four gods, including blue dragon of the east, red phoenix of the south, white tiger of the west, and black tortoise of the north) and the location will be provided while focusing on the Yeoju Headquarters Temple Complex. This study supports the feasibility of further Feng-Shui studies of the Yeoju Headquarters Temple Complex based on traditional geomancy books that focusing on Hyeonggi (Energy of Form) Theory.

A Study on the Fengshui Shapes of the Four Propitious Sites Recorded in The Canonical Scripture (『전경』에 기록된 사명당(四明堂)의 풍수 물형(物形) 연구)

  • Shin Young-dae
    • Journal of the Daesoon Academy of Sciences
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    • v.41
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    • pp.133-178
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    • 2022
  • This study investigates if four propitious sites (四明堂, sa myeongdang) identified in Daesoon Jinrihoe's The Canonical Scripture correspond with the orthodox descriptions of dragon, energy hub, sand, and water (龍穴砂水, yong hyeol sa su) which are held by Fengshui as conditions necessary for that specific designation. In this study, these conditions, based on the shape of the site-formations, were observed via through on-site surveys and the application of the theories presented by traditional books on Fengshui. First, the dragon veins (龍脈, yongmaek) of the energy hub of the Five Immortals Playing Baduk (五仙圍碁穴 oseonwigi- hyeol) on Mount Hoemun in Sunchang consists of solid soil, is like a spiderweb, rises and lays prone, winds in every direction, and looks almost disconnected while actually remaining connected. Second, the Fengshui characteristics of the energy hub of Worship Held by Buddhist Monks from Abroad (胡僧禮佛穴, hoseungyebul- hyeol) on Mount Seungdal in Muan is that the branching feet (枝脚 jigak) support the mountain range by forming a valley to the left and right of the dragon veins that stretch from the peak of Mount Seungdal. Also, the direction-changing helm (橈棹 yodo) supports the mountain range solidly can be said to be well-developed. It is likewise noted that there is an excellent change in dragon veins in that exhibit curvature that spans being high, low, rising, and lying. This makes it appear as though the dragon is wriggling back and forth. Third, the state of the energy hub of Celestial Maidens Weaving Silk (仙女織錦穴, seonnyojikgeum-hyeol) on Sonryong Ridge in Jangseong County demonstrates overall harmony between mountain and water as it is near Mount Ju and Mount An and has a solid water outlet to which it is tightly fastened such that its energy does not leak out. Meanwhile, the positioning of its blue dragon of the east, red phoenix of the south, white tiger of the west, and black tortoise of the north is so intimate that its long flow can be said to be spinning. The Songryong Ridge area where energy hub was formed between soil and bedrock is the right land for a great favored location as it corresponds with Fengshui logic in an exemplary manner. Fourth, the Fengshui characteristics of the energy hub of Subjects Receiving the Imperial Command (群臣奉詔穴, gunshinbongjo-hyeol) at Baerye-jeon Field in Taein can be described as embracing the village snuggly as it centers around Mount Wangja, and its blue dragon and white tiger respond to each other. Additionally, a clear distinction between host and guest is noticeable in the positions of Mount Ju and Mount An. The flowing body of water in front of the village wraps around that village as a Horizontal Water Formation (橫水局, hoengsuguk), and vigorous vapor from the earth draws breath as the yin-yang energy of the landscape courses through the earth. As dragon veins, the range of the mountain, are like the blood vessels within the human body and the dragon is compared to the limbs, the energy hub of Subjects Receiving the Imperial Command at Baerye-jeon Field in Taein can be identified as a favored location that was formed directly by the sky and earth.

Variations in Morphological and Geochemical Characteristics in Manganese Nodules from the East Siberian Arctic Shelf with Varying Water Depths (동시베리아해 대륙붕에서 산출되는 망가니즈단괴의 수심에 따른 형태학적·지화학적 특성 변화)

  • Hyo-Jin Koo;Hyen-Goo Cho;Sangmi Lee;Gi-Teak Lim;Hyo-Im Kim
    • Economic and Environmental Geology
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    • v.56 no.1
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    • pp.1-11
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    • 2023
  • In this study, we explore the morphological and geochemical characteristics for 440 manganese nodules collected from two different water depths [ARA12B-St52 (150 m, n = 239) and ARA12B-St58i (73 m, n = 201)] on the continental shelf of the East Siberian Sea from the ARA12B expedition in 2021. We also discussed the variations in the characteristics of manganese nodules with varying water depths in the Arctic Sea. The sizes of the nodules are generally greater than 3 cm at both sites. However, there is an obvious difference in the morphology with water depths. For the nodules collected at 150 m, brown-black colored tabular, tube, and ellipsoidal shapes with a rough surface texture are dominant. On the other hand, yellow-brown tabular shapes with a smooth surface texture are common for the nodules collected at 73 m. Furthermore, the slope of trend line between size and weight is significantly different at both sites: particularly, the slopes of nodules at 150 and 73 m are 1.60 and 0.84, respectively. This indicates the difference in the internal structure, porosity, and constituting elements between both nodules. Micro X-ray Flourescence (µ-XRF) results clearly demonstrate that the internal textures and chemical compositions are different with water depths. The nodules at 150 m are composed of a thick Mn-layer and a thin Fe-layer centered on the nucleus, while the nodules at 73 m are alternately grown with thin Mn- and Fe- layers around the nucleus. The average chemical compositions obtained by µ-XRF are 40.6 wt% Mn, 5.2 wt% Fe, and 7.9 Mn/Fe ratio at 150 m, and 10.3 wt% Mn, 19.0 wt% Fe, and 0.6 Mn/Fe ratio at 73 m. The chemical compositions of the nodules at 150 m are similar to those of nodules from the Peru Basin in the Pacific Ocean, while the compositions of the nodules at 73 m are similar to those of nodules from the Cook Islands or the Baltic Sea. The observed morphological and geochemical characteristics of the nodules show a clear difference at the two sites, which indicates that the aqueous conditions and formation processes of the nodules in the Arctic Sea vary with the water depths.

Study of the Sludge Formation Mechanism in Advanced Packaging Process and Prevention Method for the Sludge (어드밴스드 패키징 공정에서 발생할 수 있는 슬러지의 인자 확인 및 형성 방지법의 제안)

  • Jiwon Kim;Suk Jekal;Ha-Yeong Kim;Min Sang Kim;Dong Hyun Kim;Chan-Gyo Kim;Yeon-Ryong Chu;Neunghi Lee;Chang-Min Yoon
    • Journal of the Korea Organic Resources Recycling Association
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    • v.31 no.1
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    • pp.35-45
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    • 2023
  • In this study, the sludge formation in the wastewater drain from the advanced packaging process mechanisms are revealed as well as the key factors, materials, and sludge prevention methods using surfactant. Compared with that of conventional packaging process, advanced packaging process employ similar process to the semiconductor fabrication process, and thus many processes may generate wastewater. In specific, a large amount of wastewater may generate during the carrier wafer bonding, photo, development, and carrier wafer debonding processes. In order to identify the key factors for the formation of sludge during the advanced packaging process, six types of chemicals including bonding glue, HMDS, photoresist (PR), PR developer, debonding cleaner, and water are utilized and mixing evaluation is assessed. As a result, it is confirmed that the black solid sludge is formed, which is originated by the sludge seed formation by hydrolysis/dehydration reaction of HMDS and sludge growth via hydrophobic-hydrophobic binding with sludge seed and PR. For the sludge prevention investigation, three surfactants of CTAB, PEG, and shampoo are mixed with the key materials of sludge, and it is confirmed that the sludge formations are successfully suppressed. The underlying mechanism behind the sludge formation is that the carbon tails of the surfactant bind to PR with hydrophobic-hydrophobic interaction and inhibit the reaction with HMDS-based slurry seeds to prevent the sludge formation. In this regard, it is expected that various problems like clogging in drains and pipes during the advanced packaging process may effectively solve by the injection of surfactants into the drains.