• Bulletin of the Korean Chemical Society
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• v.35 no.5
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• pp.1455-1459
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• 2014

We report a sensitive and reliable FRET-based nanotechnology assay for efficient detection and quantification of bisulfite-unmodified or modified DNA. Bisulfite-untreated DNA or bisulfite-treated DNA is subjected to PCR amplification with biotin-conjugated primers so that the amounts of bisulfite-untreated and treated DNA can be differentiated. Streptavidin-coated quantum dots (QDs) are used to capture biotinylated PCR products intercalated with SYBR Green, enabling FRET measurement. Key features of our method include its low intrinsic background noise, high resolution, and high sensitivity, enabling detection of as little as 1.75 ng of bisulfite-untreated DNA in the presence of an approximately 1,000-fold excess of bisulfite-untreated DNA compared to bisulfate-treated DNA, with the use of PCR reduced (as low as 15 cycles). SYBR Green as an intercalating dye as well as a FRET acceptor allows for a single-step preparation without the need for primers or probes to be chemically conjugated to an organic fluorophore. Multiple acceptors per FRET donor significantly enhance the signal-to-noise ratio as well. In consideration of the high relevance of bisulfite treatment to DNA methylation quantitation, our system for FRET measurement between QDs and intercalating dyes can be generally utilized to analyze DNA methylation and to potentially benefit the scientific and clinical community.

• Analytical Science and Technology
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• v.13 no.3
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• pp.304-308
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• 2000

The iodide was recovered from a simulated spent fuel to the sodium bisulfite aqueous solution. It was discussed that the trace iodide (below 1 ppm) was determined without the matrix effect of 0.1 M sodium bisulfite and 1 mM $HNO_3$ in aqueous solution by ion chromatography with UV detection. AS4A-SC (DIONEX) column and UV-absorption spectrophotometer were used. The UV-absorption spectra of sodium bisulfite, nitric acid and iodide were obtained, and then 230 nm was selected as an absorption wavelength for iodide determination. 0.1 M NaCl eluent was optimum condition. In this condition the calibration curve of iodide was obtained on the range of about 0-1,000 ppb. The linear coefficient was 0.99993 and the detection limit was 5 ppb. The relative standard deviation was 1.26%.

• Journal of the Korean Wood Science and Technology
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• v.22 no.1
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• pp.34-39
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• 1994

Effects of Mg-base acid sulfite and Mg- or Na-base bisulfite on pine bark cooking were examined. In the presence of 75 % free acid at 145 $^{\circ}C$, the rate of cooking and delignification was improved with the increase of total acid. However, the delignification could not be achieved by 50 % or more under these cooking conditions. When cooked with 50~65 % free acid and at 155~165 $^{\circ}C$, the rate of cooking and delignification was remarkably improved. Thus, the bark was delignified up to 73 % when cooked for 2 hours in the presence of 50 % free acid and 24 % total acid. Na-base bisulfite was slightly more effective than Mg-base bisulfite for cooking, giving 76 % delignification of pine bark. However, there was no significant difference in selectivity of delignification between Na- and Mg-base bisulfite cooking.

• Genomics & Informatics
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• v.9 no.3
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• pp.136-137
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• 2011

Methylation of cytosine is a post-synthesis modification that does not affect the primary DNA sequence but greatly influences gene expression level and phenotypes of an organism. As high-throughput sequencing of bisulfite-treated DNA is the most efficient method to identify methylated sites, several tools to map sequencing reads on a reference are available. But tools to visualize and to interpret the methylation level of methylation sites are currently insufficient. Herein, we present a novel tool to visualize the methylation level of CpG sites.

• Elastomers and Composites
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• v.52 no.3
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• pp.211-215
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• 2017

Thermally expandable microspheres were used as post-treatment initiators of potassium persulfate, sodium bisulfite, and sodium sulfide in order to improve the foaming ability and whiteness when foaming a mixture of thermally expandable microsphers and poly(vinyl chloride). Potassium persulfate showed no significant influence on the foaming behavior, foam expansion, whiteness, and yellowing, whereas in the case of using sodium bisulfite. In particular, sodium bisulfite demonstrated the best efficiency with 2 wt% treatment. The thermally expandable microspheres prepared herein can provide excellent foamability and whiteness, and are expected to be applicable in various fields such as general coating and wallpaper.

• Korean Journal of Food Science and Technology
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• v.28 no.5
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• pp.940-946
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• 1996

One of the biggest Problems in making jeotkal is the reduction of its shelf-life when lowering the salt content from 20-30% to below 10%. Therefore, in order to extend the shelf-life of the low-salted jeotkal, prior to setting the minimum allowance value of sulfiting agents as food additives for fermented fish products, the preservative effects of sulfite salts on the low-salted myungranjeot (soused roe of Alaska pollack) were studied through various chemical and microbial analyses. The pHs of the low-salted Myungranjeot treated with bisulfite and metasulfite salts rapidly decreased in the biginning of fermentation, while the lactic acid contents increased constantly. Sodium bisulfite and metasulfite enhanced the production of $NH_2-N$ after 10 day-fermentation, whereas they inhibited the production of VBN, TMA and TBA, and the growth of microorganisms including fungi during fermentation. The estimated shelf-lives of low-salted myungranjeot treated with control, sodium sulfate, sodium bisulfite, and sodium metasulfite on the basis of VBN 50 mg% were about 16, 14, 20 and 24 days, respectively.

• Journal of the Korean Applied Science and Technology
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• v.30 no.3
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• pp.551-559
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• 2013

To investigate the adhesion effect of sodium bisulfite content in making blocked isocyanate, wood adhesive PB-1, PB-2, PB-3 and PB-4 containing sodium bisulfite content of 15%, 22.5%, 30% and 37.5% were synthesized respectively. As a result, when the amount of sodium bisulfite increased in adhesives, the tensile strength was found to be proportionally increased. The final adhesive mixtures were manufactured using a two-components system which are prepared by mixing two separate protein and BI solutions due to the precipitate in the adhesives. As PVA was added to adhesives to increase tenacity, the plywood dehiscence phenomenon after cold pressing process was declined. By addition of PVA, the tensile strength was improved up to $6.5{\sim}7kgf/cm^2$ with BI/protein ratio from 1:6 up to 1:8. Phase separation between milk fat and aqueous layer was disappeared after addition of emulsifier, Tween 20. Additon of Tween 20 showed tensile strength up to $5{\sim}6.5 kgf/cm^2$ at NCO/protein ratio of 1:12 ~ 1:14 without phase separation.

• Journal of the Korean Chemical Society
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• v.56 no.3
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• pp.328-333
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• 2012

A simple, convenient and practical green synthetic protocol for sodium bisulfite catalyzed multicomponent reaction of ethyl acetoacetate, hydrazine hydrate, malononitrile, and various aldehydes for the synthesis of 6-amino-4-phenyl-3-methyl-2,4-ihydropyrano[2,3-c]pyrazole-5-carbonitriles using ultrasound irradiations in solvent free condition. This method provides the advantage of operational simplicity, shorter reaction time and excellent yields making the protocol environment friendly and economically lucrative.

• Journal of Microbiology and Biotechnology
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• v.11 no.5
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• pp.819-824
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• 2001

The principal DNA modification systems of the amino-acid-producing bacteria Corynebacterium glutamicum AS019, Brevibacterium flavum BF4, and B. lactofermentum BL1 was investigated using two approaches; digestion of plasmid DNA isolated from these species TseI and Fnu4HI, and sequence analysis of the putative methyltransferase target sites following the derivatization of DNA using metabisulfite treatment. The C. glutamicum and B. flavum strains showed similar digestion patterns to the two enzymes, indicating that the target for cytidine methyltransferase recognizes 5'-GCSGC-3'(where S is either G or C). Mapping the methylated cytidine sites by bisulfite derivatization, followed by PCR amplification and sequencing, was only possible when the protocol included an additional step eliminating any underivatized DNA after PCR amplification, thereby indicating that the derivatization was not $100\%$ efficient. This may have been due to the high G0C content of this genus. It was confirmed that C. glutamicum AS019 and B. flavum BF4 methylated the cytidine in the $Gm^5CCGC$ sequences, yet there were no similar patterns of methylation in B. lactofermentum, which was consistent with the distinctive degradation pattern seen for the above enzymes. These findings demonstrate the successful application of a modified bisulfite derivatization method with the Corynebacterium species for determining methylation patterns, and showed that different species in the geneus contain distinctive restriction and modification systems.

• Proceedings of the Korea Technical Association of the Pulp and Paper Industry Conference
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• 2006.06b
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• pp.373-379
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• 2006

Fiber characteristics and fiber distribution of thermomechanical pulp(TMP), bisulfite chemithermomechanical pulp(bisulfite CTMP), neutral sulfite chemithermomechanical pulp(neutral sulfite CTMP) from kenaf(Hibiscus cannabinus L., Malvaceae) cultivar Tainug-2 cultivated in the reclaimed land of Korea were examined to use effectively nonwood fibers as an alternative raw material sources for papermaking. Yields of TMP and CTMP from kenaf were lower than those of TMP from hardwoods and CTMP from softwoods and hardwoods. Bark fibers of kenaf cultivar Tainung-2 ranged 2.04 to 2.30 mm long and $18.7{\sim}19.7{\mu}m$ width. Core fibers averaged 0.63 to 0.80 mm long and $29.5{\sim}31.4{\mu}m$ wide. Coarseness of bark fiber was higher than that of core fiber, and fiber from TMP were higher than those from both bisulfite CTMP and neutral sulfite CTMP. Curl indexes of bark fibers were higher than those of core fibers. However curl indexes were not significantly affected by the pulping conditions. Short fiber distributions were higher in core fibers from TMP and CTMP and long fiber distributions were higher in bark fibers. There was no significant difference in fiber distribution of whole and core fibers obtained from TMP and CTMP, Fibers from neutral sulfite CTMP, however, exhibited a little higher long fiber distribution. Distinct difference in anatomical characteristics was found between core and bast fibers of kenaf plant. Parenchyma cell, pith parenchyma cell and vessel were observed in core fibers and bast fiber in bast sections.