• Korean Journal of Fisheries and Aquatic Sciences
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• v.54 no.5
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• pp.585-595
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• 2021

Even though most of aquatic animal pathogens are considered opportunistic and many pose a low direct risk to personnel, all personnel working with aquatic pathogens and facilities using these organisms must comply with the regulation to prevent the release of the pathogen into the environment and causing disease in aquatic animals. First of all, in order to establish a biosafety system for aquatic pathogen, the list of microorganisms that can infect aquatic animals and humans should be drawn up according to the microorganisms encountered within national boundaries. Second, risk assessment guideline for diseases of livestock and aquatic environment is desperately needed. Third, microorganisms should be classified into risk group based on their potential impact on human and aquatic environment. Fourth, facilities handling aquatic pathogens should ensure that these pathogens are securely contained and safely handled for experimental or commercial development purposes. In conclusion, classification is based on the pathogenicity, mode of transmission and host range of the aquatic microorganisms, availability of effective preventative measures and treatments. Furthermore, risk group of aquatic pathogens should be correlated with physical containment facility requirements according to domestic characteristics.

• Journal of Plant Biotechnology
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• v.35 no.4
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• pp.291-298
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• 2008

Methylmercury, an organic derivative, is the principal form of mercury that biomagnifies and causes neurodegenerative symptoms in animals. In recent years, living modified organism (LMO) resulting from biotechnology has played a highly visible and controversial role. Despite the potential benefits of this technology, public concerns have been raised about the environmental risk of LMO. The concern on the risk from LMO release has urged efforts to evaluate and manage the risks of the LMO. To build up the capacity building of risk assessment method for LMO used environmental remediation, we engineered Solanum nigrum L, expressing the modified bacterial gene, merB, encoding organomercurial lyase. Two independently isolated transgenic lines produced merB RNA. Transgenic Solanum nigrum leaf discs expressing merB gene showed organic mercury resistance, forming shoots well on growth medium containing $0.5{\mu}M$ methylmercury (II) chloride and $1{\mu}M$ phenylmercuric acetate while control plants breached. Transgenic merB seeds germinated and grew on growth medium containing $2{\mu}M$ methylmercury (II) chloride and phenylmercuric acetate. The merB transgenic plants will be used for risk assessment of natural environment.

• Journal of Life Science
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• v.32 no.8
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• pp.641-646
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• 2022

There are concerns about the environmental release of living modified organism (LMO) maize created to increase yields. In fact, there are cases in which LMO crops for feed have been leaked in Korea to form autoite colonies, and concerns about LMO spill are intensifying. In this study, the possibility of environmental outflow and occurrence of native organisms was analyzed using maize feed and seeds distributed in Korea. In the evaluation of the possibility of spontaneous occurrence of maize in the event of an unintentional release of maize feed made by crushing maize, the incidence rate of maize was 0.01%, which was extremely low compared to the germination rate of maize seeds. A survey of the dormant rate of maize showed that all maize seeds collected every month were dead. In the germination rate test by temperature using Daehak wax corn and Kwangpyeongok, high germination rates were found at 20℃ and 30℃, and relatively low germination rates were found at 10℃ and 40℃. In addition, all germination tests showed a higher germination rate Daehak wax corn than Kwangpyeongok. The difference between domestic and overseas cultivation maize was confirmed through a survey on the agricultural properties of three varieties of maize. The data obtained through this experiment could be the basis for the evaluation of the weediness potential of environmental risk assessment and technology to suppress the occurrence of autoite in preparation for future LMO spills.

• Korean Journal of Breeding Science
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• v.42 no.1
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• pp.56-60
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• 2010

This study was carried out to develop of macro-protocol and the biosafety guide Golden Rice, modified vitamin A at large scale GMO field. Commercialization of genetically modified (GM) plants will be required the assessment of risks associated with the release of GM plants that should include a detailed risk assessment of their impacts in the environment and human health. Prior to GM plant release, applicants should provide the information on GM crops for approval. A total of $4,700m^2$ GM field, each of Golden Rice and 'Nakdong' were used for further analysis on agronomic traits and pathogenesis. Generally plant growth and panicle number in the transgenic lines were lower than in the wild type under field condition. The major agronomic traits such as plant growth, grain quality in Golden Rice were similar than in 'Nakdong'. The yield difference was due to reduced ripened rate and the 1,000 grain weight. The grain shape was similar donor plant, however, color of brown rice was different from segregation. Also the difference of pathogenesis reaction was not significant in the leaf blast and sheath blight between Golden Rice and 'Nakdong'. These results provided the agronomic data and pathogenesis for risk assessment analysis of Golden Rice and suggested that the macro-protocol could be useful to detect GM plants.

• Weed & Turfgrass Science
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• v.3 no.4
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• pp.305-311
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• 2014

The cultivation area and use of genetically modified (GM) crops have been increased continuously over the world. Seed distribution and transgenes to environmental ecosystem is one of the most important factors in risk assessment and risk management of GM crop. Safe management for the development and commercialization of GM crops is required according to The Act on Transboundary Movements of Living Modified Organisms,etc (LMO Act) in Korea. This study was conducted to setup the environmental monitoring system of GM zoysiagrass (event JG21 and JG21-MS). The monitoring was performed in 4 GMO confined fields, Sungwhan, Ochang, Jeju University and Jeju Namwon. In the result of monitoring, we could not found any gene flow and distribution of GM zoysiagrass in the 3 fields, but one spill of JG21 was found in the Namwon field in 2012. These results suggest that continuous monitoring is necessary to detect the occurrence of GM zoysiagrass for preventing genetic contamination in natural environment.

• Journal of Applied Biological Chemistry
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• v.54 no.4
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• pp.296-302
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• 2011

Insect-resistant transgenic rice was developed by inserting the mCry1Ac1 a modified gene from the soil bacterium Bacillus thuringiensis (Bt). For biosafety assessment, we studied the effects on survival of cantor Daphnia magna, a commonly used as a model organism in ecotoxicological studies. D. magna fed on Bt rice and its near non-genetically modified (GM) counterparts (Nakdong) grown in the same environment (100% ground rice suspension). The Bt rice was comfirmed to have the insertion of T-DNA and protein expression by the polymerase chain reaction and ELISA analysis. Feeding study showed similar cumulative immobility and abnormal response of D. magna between Bt rice and non-GM counterparts. 48 h-$EC_{50}$ values of Bt rice and non-GM rice showed 4,429 and 2,889 mg/L respectively. The rice no observed effect concentration (NOEC) values for D. magna was suggested 1,000 mg/L. We conclude that the tested Bt-rice and Nakdong similar cumulative immobility for D. magna the widely used model organism. We found out that there is strong possibility that the growth of Bt rice didn't affect to non-target insects.

• Korean Journal of Agricultural Science
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• v.47 no.4
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• pp.815-827
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• 2020

The epidermal growth factor (EGF) transgenic soybean was developed and biosynthesis of human epidermal growth factor (hEGF) in soybean seeds was confirmed. Also, EGF transgenic soybean were found to contain a herbicide resistance selectable marker by introduction of phosphinothricin acetyltransferase (PAT) gene from the Streptomyces hygroscopicus. For biosafety assessment, the EGF transgenic soybean expressing the EGF biosynthesis gene EGF and herbicide resistant gene PAT was tested to determine effects on survival of Cyprinus carpio, commonly used as a model organism in ecotoxicological studies. C. carpio was fed 100% ground soybean suspension, EGF soybean or non-genetically modified (GM) counterpart soybean (Gwangan). Gene expression of EGF soybean was confirmed by PCR and ELISA to have EGF/PAT. Feeding test showed that no significant differences in cumulative immobility or abnormal response between C. carpio samples fed on EGF soybean and non-GM counterpart soybean. The 48 h-EC50 values of the EGF and non-GM soybean were 1,688 mg·L-1 (95% confidence limits: 1,585 - 1,798 mg·L-1) and 1,575 mg·L-1 (95% confidence limits: 1,433 - 1,731 mg·L-1), respectively. The soybean NOEC (no observed effect concentration) value for C. carpio was suggested to be 625 mg·L-1. We concluded that there was no significant difference in toxicity for non-target organisms (C. carpio) between the EGF soybean and non-GM counterparts.

• Analytical Science and Technology
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• v.34 no.3
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• pp.99-114
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• 2021

Substance identification is the first step in implementing chemical legislation, such that subsequent hazard and risk assessments can be accurately followed. Based on the web page and related guidance documents of the European Chemicals Agency and available consortia information, the procedure for substance identification carried out in Registration, Evaluation, Authorization and Restriction of Chemicals (REACH) is surveyed. In this study, the importance of substance identification and substance sameness check, as well as the necessity of generating a substance identity profile (SIP) are considered. In addition, the SIPs of several substance types are presented, which focused on information utilization in the instrumental analysis results and organization of information to generate the SIP. Analytical science can contribute to the accurate and effective implementation of chemical regulation at the starting stage of substance identification. However, understanding of the regulation and consequent final wrap-up of analytical results as a SIP should be followed for communication among registrants in Substance Information Exchange Forum (SIEF) as well as with related authorities.

• Korean Journal of Air-Conditioning and Refrigeration Engineering
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• v.24 no.11
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• pp.813-822
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• 2012

In this study, the multizone simulation for biosafety of BSL3 lab. and energy simulation are carried out simultaneously by using linked model of CONTAM and TRNSYS. In BSL3 lab., annual energy consumption is approximately five to ten times more than the magnitude of the office building. This is because required air change rate is extremely large and it is difficult to maintain room pressure difference efficiently. To maintain pressure difference between laboratory rooms through sealing condition of doors and proper airflow control is significant. In this study, to predict indoor environment of the BSL3 lab.(Influenza A research lab.), the multizone simulation for four kinds of biohazard scenario is also performed as part of risk assessment. Multizone and energy simulation results by using linked model show that these approaches are used as a tool for the energy efficient design and operation method for the safer BSL3 lab. facilities.

• Journal of the Optical Society of Korea
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• v.18 no.2
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• pp.174-179
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• 2014

Since in vitro neural recording and imaging applications based on a surface plasmon resonance (SPR) technique have expanded dramatically in recent years, cytotoxicity assessment to ensure the biosafety and biocompatibility for those applications is crucial. Here, we report the cytotoxicity of the SPR substrate incorporating a flint glass whose refractive index is larger than that of a conventional crown glass. A high refractive index glass substrate is essential in neural signal detection due to the advantages such as high sensitivity and wide dynamic range. From experimental data using primary hippocampal neurons, it is found that a lead-based flint glass is not appropriate as a neural recording template although the neuron cells are not directly attached to the toxic glass. We also demonstrate that the adhesion layer between the glass substrate and the gold film plays an important role in achieving the substrate stability and the cell viability.