Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Selection of transgenic Solanum nigrum L. used environmental remediation expressing organomercurial lyase

Organomercurial lyase 유전자를 도입한 환경정화용 형질전환 까마중(Solanum nigrum) 선발

  • Choi, Kyung-Hwa (Environmental Biosafety Division, National Institute of Environmental Research) ;
  • Kim, Yong-Ho (Environmental Biosafety Division, National Institute of Environmental Research) ;
  • Chung, Hyen-Mi (Environmental Biosafety Division, National Institute of Environmental Research) ;
  • Choi, Young-Im (Biotechnology Division, Korea Forest Research Institute) ;
  • Noh, Eun-Woon (Biotechnology Division, Korea Forest Research Institute) ;
  • Kim, Hyun-Soon (Plant genome research center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Jeon, Jae-Heung (Plant genome research center, Korea Research Institute of Bioscience and Biotechnology)
  • 최경화 (국립환경과학원 환경바이오안전과) ;
  • 김용호 (국립환경과학원 환경바이오안전과) ;
  • 정현미 (국립환경과학원 환경바이오안전과) ;
  • 최영임 (국립산림과학원 생물공학과) ;
  • 노은운 (국립산림과학원 생물공학과) ;
  • 김현순 (한국생명공학연구원 식물유전체연구센터) ;
  • 전재흥 (한국생명공학연구원 식물유전체연구센터)
  • Published : 2008.12.31
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Abstract

Methylmercury, an organic derivative, is the principal form of mercury that biomagnifies and causes neurodegenerative symptoms in animals. In recent years, living modified organism (LMO) resulting from biotechnology has played a highly visible and controversial role. Despite the potential benefits of this technology, public concerns have been raised about the environmental risk of LMO. The concern on the risk from LMO release has urged efforts to evaluate and manage the risks of the LMO. To build up the capacity building of risk assessment method for LMO used environmental remediation, we engineered Solanum nigrum L, expressing the modified bacterial gene, merB, encoding organomercurial lyase. Two independently isolated transgenic lines produced merB RNA. Transgenic Solanum nigrum leaf discs expressing merB gene showed organic mercury resistance, forming shoots well on growth medium containing $0.5{\mu}M$ methylmercury (II) chloride and $1{\mu}M$ phenylmercuric acetate while control plants breached. Transgenic merB seeds germinated and grew on growth medium containing $2{\mu}M$ methylmercury (II) chloride and phenylmercuric acetate. The merB transgenic plants will be used for risk assessment of natural environment.

수은은 산업화될수록 방출량이 많아지고 자연생태계의 먹이사슬에 의한 생물학적 축적에 의하여 결국 인간에게 강한 독성을 나타내게 된다. 최근 중금속 무독화관련 유전자를 도입한 유전자변형식물체를 이용하여 중금속의 독성을 제거하거나 저감하려는 연구가 활발히 이루어지고 있다. 그러나 유전자변형생물체는 자연생태계에 미치는 환경위해성을 평가하여 안전성을 검증한 후 환경에 방출해야만 한다. 환경정화용 유전자변형생물체의 환경위해성 평가기술개발연구에 이용하기 위한 형질전환 식물체 생산을 위하여 까마중에 유기수은 (organic mercury)을 무기수은 (ionic mercury)으로 전환시켜서 독성을 저감시키는 organomercurial lyase (merB) 유전자를 도입하여 형질전환 시켰다. 유전자 도입 및 발현이 확인된 2개의 형질전환 라인 (merB1, merB4)은 유기수은제제인 MMC $0.5{\mu}M$, PMA $1{\mu}M$에서 저항성을 나타내었다. 또한 형질전환 1세대 종자도 $2{\mu}M$ MMC와 PMA에 저항성을 나타내는 것을 확인하였다. 향후 이들 형질전환체를 이용하여 환경 정화용 유전자변형생물체의 환경위해성 평가방법 개발 연구를 수행 할 것이다.

Keywords

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