• Horticultural Science & Technology
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• v.17 no.4
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• pp.500-502
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• 1999

This study was carried out to survey the distribution of Korean native Sedum rotundifolium and environmental conditions of their native habitats. Sedum rotundifolium could be found only at the Mt. Juwang and its vicinity, Okgae valley. And most of Sedum rotundifolium plants in habitats were positioned between the chasm of cliffs in middle mountainous area having altitudes ranging about 250~450 m. Most of soil conditions in native habitats were somewhat arid, and light intensities were ranged from 1 to 90 % of those at the open area. But relative humidity, air temperature and soil temperature showed little difference compared with those of open area. Soil acidities were ranged from pH 5.0 to 7.0, and relatively high amount of humus, total $P_2O_5$, Ca, and Mg were measured at habitat area. On the other hand, growth of Sedum rotundifolium was significantly different according to habitat sites.

• Journal of Life Science
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• v.19 no.2
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• pp.228-233
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• 2009

The purpose of this study is to evaluate the activities of five different antioxidant enzymes in various tissues of Papilio xuthus during pupal stage. Superoxide dismutase (SOD) activity in haemolymph was the highest just after pupation and then decreased gradually until 7 days after pupation but the activity in other tissue was constant during metamorphosis. This result indicates that primary antioxidant process of reactive oxygen species proceed in haemolymph. Catalase (CAT) activity in studied tissues was also the highest just after pupation and its relative activity was also high during pupal stage, suggesting that CAT is the primary enzyme in catalysis of hydrogen peroxide. Glutathion peroxidase (GPX) activity was constant and its relative activity was very low in all tested tissues. Glutathione S-transferase (GST) activity in haemolymph was high at 3 days and 5 days after pupation, and the activity in fat body was the highest at the 1 day after pupation and then decreased gradually for 7 days after pupation. Glutathion reductase (GR) activity in haemolymph and fat body was high at 1 day after pupation, but relatively low GR activity was detected in the rest tissues. Based on these results, GST activity was higher than that of GPX and GR, and it is also believed that GST was more involved in reduction process through lipid peroxidation than GPX.

• Journal of Life Science
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• v.24 no.11
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• pp.1231-1237
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• 2014

The objective of this study was to evaluate the safety and functional properties of four potential probiotic strains isolated from Kimchi, traditional Korean fermented vegetables. Based on being higher tolerance to bile salts and showing higher acid resistance or hydrophobic properties, one Lactobacillus arizonensis strain (BCNU 9032) and three L. brevis strains (BCNU 9037, BCNU 9098 and BCNU 9101) were selected in the screening experiment. All strains can survived up to 99% after 3h culture in pH 2.5 and resistant to 1% bile salts. These strains also showed good antimicrobial activities against a number of food borne pathogens, especially against Escherichia coli and Shigella sonnei. The ability to lower cholesterol levels of L. arizonensis BCNU 9032 and L. brevis 9037 were demonstrated by bile salt hydrolytic activity and cholesterol assimilation tests. Moreover, L. brevis BCNU 9098 and BCNU 9101 showed higher adherence to Caco-2 cells (12.76 and 11.86%, respectively) than Lactobacillus rhamnosus GG, a commercial probiotic strain used worldwide. The results suggest that these strains could be used as probiotics.

• Journal of Biosystems Engineering
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• v.36 no.2
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• pp.79-88
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• 2011

The purpose of this study was to analyze power requirement of an agricultural tractor by major field operations. First a survey was conducted to obtain annual usage ratio of agricultural tractor by field operation. Plowing, rotary tillage, and loader operations were selected as major field operations of agricultural tractor. Second, a power measurement system was constructed with strain-gauge sensors to measure torque of four driving axles and a PTO axle, speed sensors to measure rotational speed of the driving axles and an engine shaft, pressure sensors to measure pressure of hydraulic pumps, an I/O interface to acquire the sensor signals, and an embedded system to calculate power requirement. Third, the major field operations were experimented under fields with different soil conditions following planned operation paths. Power requirement was analyzed during the total operation period consisted of actual operation period (plowing, rotary tillage, and loader operations) and period before and after the actual operation (3-point hitch operating, forward and reverse driving, braking, and steering). Power requirement of tractor major components such as driving axle part, PTO part, main hydraulic part, and auxiliary hydraulic part were measured and calculated to determine usage ratio of agricultural tractor power. Results of averaged power requirement for actual field operation and total operation were 23.1 and 17.5 kW, 24.6 and 19.1 kW, and 14.9 and 8.9 kW, respectively, for plowing, rotary tillage, and loader operations. The results showed that rotary tillage required the greatest power among the operations. Averaged power requirement of driving axles, PTO axle, main hydraulic part, and auxiliary part during the actual field operation were 8.1, 7.8, 3.4, and 1.5 kW, respectively, and the total requirement power was about 70 % (20.8 kW) of the rated power. Averaged power requirement of driving axles, PTO axle, main hydraulic, and auxiliary hydraulic for the total operation period were 6.5, 6.0, 2.1, 0.9 kW, respectively, and total requirement power was about 52 % (15.5 kW) of the rated power. Driving axles required the greatest amount of power among the components.

• Journal of Biosystems Engineering
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• v.36 no.4
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• pp.243-251
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• 2011

Purpose of this study was to analyze power requirement of an agricultural tractor for baler operation. First, a power measurement system was developed and installed in a 75 kW agricultural tractor. Strain-gages with a telemetry system were used to measure torques of transmission and PTO input shafts. An engine tachometer was used to measure rotational speed of transmission and PTO input shafts. The measurement system also included pressure sensors to measure pressure of hydraulic pumps, an I/O interface to acquire the sensor signals, and an embedded system to determine power requirements. Second, field experiments were conducted at two PTO speed levels, and proportion of utilization ratio of rated engine power and power consumption of major parts (transmission input shaft, PTO input shaft, main hydraulic pump, and auxiliary hydraulic pump) were analyzed. Results of usage proportion of engine power for PTO speed level 1 and 2 were 4.1 and 2.2%, 31.5 and 16.3%, 49.6 and 59.7%, 14.4 and 20.8%, and 0.4 and 1.0%, respectively, for ratio of measured engine power to rated engine power of less than 25%, 25 ~ 50%, 50 ~ 75%, 75 ~ 100%, and greater than 100%. The results showed that the usage proportion increased in the range with the ratio of power requirement to rated engine power of over than 50% when the PTO gear was shifted from P1 to P2. Averaged engine power requirement for baling operation, tying and discharging operation, and total operation were 43.3, 37.3, and 42.0 kW and 49.0, 37.0, and 47.4 kW, respectively, for PTO speed level 1 and 2. Paired t-test showed significant difference in power consumption of engine, transmission input shaft, and PTO input shaft for different PTO speed levels. Therefore, the power consumption of engine for baler operation increased when the PTO gear was shifted from P1 to P2. It was indicated that the power requirement of tractor was affected by the PTO rotational speed for baler operation.

• Research in Plant Disease
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• v.10 no.4
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• pp.248-259
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• 2004

A total 115 isolates of Fusarium species from ginseng roots of 'rotted', and soils collected during 1982-1985 in Korea, were identified and classified into 11 species with the Snyder & Hansen System (with reference to Gerlach-Nirenberg's Modified System). The most dominant of these species were F. solani (55 isolates), F. oxysporum (35 isolates), and F. moniliforme (10 isolates) sensu Snyder & Hansen. The other 8 species (15 isolates) were very rarely isolated and previously identified as F. roseum sensu Snyder & Hansen (1945); these were F. equiseti, F. avenaceum, F. graminum, F. arthrosporioides, F. sambucinum, F. reticulatum, F. semitectum and F. poa. Tested for the ability to infect the roots of ginseng (3 yr. old plants) in field condition with the mycelial inoculum, only one isolate of F. solani (34 isolates tested) and one isolate of F. oxysporum (24 isolates tested) were weakly pathogenic to ginseng roots. Any of the isolates (7 isolates tested) of F. moniliforme [Liseola section] were not pathogenic to ginseng. However, all the isolates of tested of the species of Phytophthora cactorum, Pythium ultimum, and Cylindrocarpon destructans were highly pathogenic to ginseng roots. The species of Fusarium solani and Cylindrocarpon destructans were supposed to be a host dominant disease agent in ginseng plant.

• The Plant Pathology Journal
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• v.33 no.5
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• pp.467-477
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• 2017

A nationwide survey was performed to investigate the current incidence of viral diseases in Korean sweet potatoes for germplasm and growing fields from 2011 to 2014. A total of 83.8% of the germplasm in Korea was infected with viruses in 2011. Commercial cultivars that were used to supply growing fields were infected at a rate of 62.1% in 2012. Among surveyed viruses, the incidence of five Potyvirus species that infect sweet potato decreased between 2012 and 2013, and then increased again in 2014. Representatively, the incidence of Sweet potato feathery mottle virus (SPFMV) was 87.0% in 2012, 20.7% in 2013 and then increased to 35.3% in 2014. Unlike RNA viruses, DNA viruses were shown to decrease continuously. The incidence of Sweet potato leaf curl virus (SPLCV) was 5.5% in 2003, 59.5% in 2011, and 47.4% in 2012. It then decreased continuously year by year to 33.2% in 2013, and then 25.6% in 2014. While the infection rate of each virus species showed a tendency to decline, the virus infection status was more variable in 2013 and 2014. Nevertheless, the high rate of single infections and mixed infection combinations were more variable than the survey results from 2012. As shown in the results from 2013, the most prevalent virus infection was a single infection at 27.6%, with the highest rate of infection belonging to sweet potato symptomless virus-1 (SPSMV-1) (12.9%). Compared to 2013, infection combinations were more varied in 2014, with a total of 122 kinds of mixed infection.

• Research in Plant Disease
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• v.23 no.4
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• pp.322-333
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• 2017

In June and July 2015 and 2017, typical signs and symptoms of brown rot were observed on the fruit of Japanese apricot, peach, apricot, Japanese plum, and sweet cherry with incidence levels of 2-5% in Jeonju and Imsil, Korea. Early symptoms were small, circular, light brown spots that eventually destroyed entire fruit. Small sporodochia later appeared on the surface. Conidia isolated from each host were one-celled, hyaline, lemon-shaped and borne in branched monilioid chains. The optimal temperature range for hyphal growth of all the isolates was $20-25^{\circ}C$. The growth of hyphae was faster on potato dextrose agar and oatmeal agar than others. Multiple alignments using the ITS sequences from different host showed that they matched each other (100%). The ITS sequences showed 100% identity to those of M. fructicola. Based on the morphological characteristics and phylogenetic analysis via internal transcribed spacer (ITS), all the isolate was identified as M. fructicola. Pathogenicity of representative isolates was proved by artificial inoculation, fulfilling Koch's postulates. This is the first confirmed report on brown rot caused by M. fructicola on stone fruit in Korea.

• Research in Plant Disease
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• v.23 no.4
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• pp.306-313
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• 2017

Recently fire blight occurred in the Republic of Korea and eradication program for the disease has been executed since then. Specificity and detection sensitivity of the 2 antibody-based diagnostic strips to Korean isolates of Erwinia amylovora (Ea) and their application for on-site diagnosis were evaluated in this study. Ea AgriStrip, a commercial diagnostic kit, and EB strip, developed in this study, reacted positively to the all tested Korean Ea strains and also to most of Erwinia pyrifoliae (Ep) strains causing black shoot blight. They reacted negatively to all Pusedomonas syringae pv. syringae (Pss) strains that cause shoot blight on apple. Detection sensitivity was similar between the 2 strips. For on-site diagnosis, the two strips reacted positively only to the extractions of the fire-blighted samples on all fire blight occurred orchards except one orchard at which on-site diagnosis was carried out at winter time. In addition, they reacted positively to the black-shoot blighted extractions from the black shoot blight occurred apple orchard. These results suggest that both EB strip and Ea AgriStrip would be useful for on-site diagnosis of fire blight in Korea.

• Korean Journal of Poultry Science
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• v.43 no.1
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• pp.21-29
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• 2016

A total of 144 one-day-old male broilers (ROSS ${\times}$ Ross 308) ($42{\pm}0.1g$) were used in a completely randomized design and allotted to one of three dietary treatments to give 6 replicates per treatment (8 birds per cage). The three watery dietary treatments were 1) a corn-soybean meal-based relation [Negative control (NC); no antimicrobial compounds added] with tab water, 2) 8 ppm liquid-alpha-lipoic acid [Positive control (PC)] or 3) 100 ppm liquid-resveratrol. All dietary treatments were continuously provided as a liquid form. Birds were housed in a battery cage (n=48), and were offered liquid dietary treatments at all times. The ambient temperature was maintained at $32{\pm}1^{\circ}C$ for the first 3 weeks and decreased gradually to $28{\pm}1^{\circ}C$ by the end of the experiment (d 35) to induce moderate heat stress. One bird per pen (n=6) was euthanized via cervical dislocation at day 21 to obtain terminal ileum to measure villous height and crypt depth, while another bird per pen (n=6) was similarly euthanized at day 35 and used to harvest breast meat and drumsticks to evaluate meat quality traits. Birds fed liquid-resveratrol did not improve (P>0.05) body weight and average daily gain compared with those fed other dietary treatments from day 1 to day 35. Birds fed liquid-resveratrol had no effect (P>0.05) on villous height compared with birds were in other dietary treatments at day 21. Liquid-resveratrol had no effect (P>0.05) on thiobarbituric acid reactive substance (TBARS) in drumsticks compared with other dietary treatments at day 35. Furthermore, birds fed liquid-resveratrol had no effect (P>0.05) on DPPH radical scavenging activity both in breast meat and drumsticks compared with those fed other dietary treatments at day 35. Morphological changes of ileum were not observed by immunofluorescence, and the level of occludin protein from ileum extracts also did not differ among groups. Our results suggested that liquid form of antioxidant compounds used in the current study such as alpha-lipoic acid (8 ppm) or resveratrol (100 ppm) did not improve growth performance, meat quality traits and histology of terminal ileum compared with those fed negative control in broiler chickens under moderate heat stress for 35 days.