• Journal of Soil and Groundwater Environment
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• v.20 no.2
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• pp.22-31
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• 2015

In the present study, surface of laccase producing Ceriporia lacerata was modified using 4-bromobutyryl chloride and polyethylenimine. The modified biomass was freeze dried and utilized as a biosorbent for the removal of Ni(II) from aqueous solution. The physicochemical properties of the biosorbent were analyzed using scanning electron microscopy and Fourier transform infrared spectroscopy. Batch experiments were carried out as a function of contact time (0-60 min), pH (2 to 8), adsorbent dosage (25-150 mg), and initial Ni(II) concentration (25-125 mg/L). The results indicate that surface modified biosorbent effectively adsorbed (9.5 mg/0.1 g biomass) Ni(II) present in the solution. The equilibrium adsorption data were modeled with different kinetic and isotherm models. The Ni(II) adsorption followed pseudo-first-order kinetics (R² = 0.998) and Langmuir isotherm (R² = 0.994) model. Hydroxyl and carbonyl functional groups present in biomass play a major role in the adsorption of Ni(II). The adsorbed Ni(II) from the biosorbent was successfully desorbed (85%) by 1M HCl. The results of the study indicate that the surface modified C. lacerate biomass could be used for the treatment of Ni(II) contaminated ground waters.

• Food Science and Biotechnology
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• v.17 no.6
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• pp.1391-1395
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• 2008

Leuconostoc citreum HJ-P4 is a strain isolated for kimchi fermentation with its low temperature-adapted growth feature and its high dextransucrase activity. The detailed characteristics of cell growth and dextran sucrase activities were investigated at various environmental conditions such as temperatures, pHs, salts, and raw ingredients. This strain showed almost 2-fold higher maximal cell concentration ($X_{max}$) than that of the type culture Leuconostoc mesenteroides B-512F at $10^{\circ}C$. The $X_{max}$ of the strain was maximum at pH 7 and the cell growth was inhibited by salts in a dose-dependent mode up to 7%. Addition of pepper (<6%), garlic (<10%), and ginger (<2%) in kimchi gave no inhibition effect on the growth of HJ-P4. Dextransucrase synthesized by this strain retained over 80% of its maximum activity at $10^{\circ}C$ showing a comparable cold-adapted feature to its host microbe. This culture can be used as a starter culture in the industrial kimchi production giving desirable functions and predominance at low temperature.

• Korean Journal of Food Science and Technology
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• v.34 no.6
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• pp.1085-1090
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• 2002

Leuconostoc mesenteroides, the major bacterium in the initial phase of lactate-fermentation in kimchi, produces lactic acid, acetic acid, mannitol, and $CO_2$. It also secrets dextransucrase, which catalyzes the transfer reaction of glucose from sucrose to maltose, synthesizing mainly panose ($6^_2-{\alpha}-D-glucopyranosylmaltose)$, a probiotic oligosaccharide. To use the strain as a starer culture to produce high amount of panose during kimchi fermentation, we screened psychrotrophic strains showing fast growth rate at low temperature among the isolates of Leuconostoc sp. and selected two strains showing high dextransucrase activity. The strains were identified as Leuconostoc mesenteroides, which can be used as function added-starters for lactate-fermented foods.

• The Plant Pathology Journal
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• v.34 no.6
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• pp.470-479
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• 2018

The rice blast pathogen Magnaporthe oryzae is a global threat to rice production. Here we characterized RHO2 gene (MGG_02457) that belongs to the Rho GTPase family, using a deletion mutant. This mutant ${\Delta}Morho2$ exhibited no defects in conidiation and germination but developed only 6% of appressoria in response to a hydrophobic surface when compared to the wild-type progenitor. This result indicates that MoRHO2 plays a role in appressorium development. Furthermore, exogenous cAMP treatment on the mutant led to appressoria that exhibited abnormal morphology on both hydrophobic and hydrophilic surfaces. These outcomes suggested the involvement of MoRHO2 in cAMP-mediated appressorium development. ${\Delta}Morho2$ mutation also delayed the development of appressorium-like structures (ALS) at hyphal tips on hydrophobic surface, which were also abnormally shaped. These results suggested that MoRHO2 is involved in morphological development of appressoria and ALS from conidia and hyphae, respectively. As expected, ${\Delta}Morho2$ mutant was defective in plant penetration, but was still able to cause lesions, albeit at a reduced rate on wounded plants. These results implied that MoRHO2 plays a role in M. oryzae virulence as well.

• Korean Journal of Plant Taxonomy
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• v.40 no.2
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• pp.130-133
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• 2010

An unrecorded species of Oldenlandia brachypoda DC. is newly reported from Seogwipo-si, Jejudo, This species is distinguished from Oldenlandia diffusa, the related taxa by the surface and length of the pedicel, the number of flowerd, the shape of the calyx and capsule. The new Korean name 'je-ju-baeg-un-pul', was given considering the collect locality. The illustration, description and key of characters are provided.

• KSBB Journal
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• v.25 no.4
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• pp.311-318
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• 2010

Lactic acid bacteria display a relatively simple metabolism wherein the sugar is converted mainly to lactic acid. The extensive knowledge of metabolic pathways and the increasing information of the genes involved allows for the rerouting of natural metabolic pathways by genetic and physiological engineering. In this contribution, the lactic acid bacteria as an efficient cell factory for different (food) ingredients will be presented. The emphasis will be on some successful examples of metabolic engineering and on the physiology of these bacteria, which makes them so suitable as a cell factory.

• Proceedings of the PSK Conference
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• 2003.04a
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• pp.257.2-258
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• 2003

Schizandra chinensis known as OMIJA belongs to Schizandraceae family, and is being used in the formulation of traditional medicine. Various column chromatographies with various solvent systems were used to isolate its compounds. To identify compounds isolated, instrumental analysis methods such as NMR and MS were employed. (omitted)

• Korean Journal of Plant Taxonomy
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• v.41 no.3
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• pp.271-279
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• 2011

The morphological characteristics of the Korean Scopolia japonica Maxim., commonly known as 'Mi-chi-gwang-i-pul' in Korean, were re-evaluated in comparison with results from previous research, while the distribution map was formulated according to existing flora and voucher specimens. The result of the re-evaluation has shown that there are some distinctive discrepancies compared to the previous reports of some characteristics such as the color of the basal stem, numbers of lateral veins, apex shape of calyx and corolla including the color of corolla. The Korean Scopolia japonica Maxim is found throughout Korea except Jeju-do province. The intra-specific species such as S. parviflora (Dunn.) Nakai, S. lutescens Y.N. Lee and S. kwangdokensis Y.N. Lee were treated as synonyms of S. japonica Maxim. in this research.

• Mycobiology
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• v.40 no.4
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• pp.258-262
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• 2012

cDNA of the glx1 gene encoding glyoxal oxidase (GLX) from Phanerochaete chrysosporium was isolated and expressed in Pichia pastoris. The recombinant GLX (rGLX) produces $H_2O_2$ over 7.0 nmol/min/mL using methyl glyoxal as a substrate. Use of rGLX as a generator of $H_2O_2$ improved the coupled reaction with recombinant manganese peroxidase resulting in decolorization of malachite green up to $150{\mu}M$ within 90 min.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2018.10a
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• pp.126-126
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• 2018

Many of researches have revealed that human intestinal microbiota is related to health. Several diseases like obesity, diabetes, and hypertension are affected by the microbiota directly and indirectly. So, interventions with food and drug have been tried to change a composition of the microbiota to better condition. However, few natural medicines have elucidated to date. To understand an influence on microbiota by plant materials including Glycyrrhizae Radix, the extract of medicines were administered to mice and the feces were collected before and after the administration. The feces were analyzed by terminal restriction fragment length polymorphism (T-RFLP). The changes in composition of mice gut microbiota were detected and analyzed. The data could be utilized to further study about biological activities of the plant medicines.