• 멤브레인
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• 제7권4호
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• pp.175-182
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• 1997

혐기-호기의 생물반응조 공정과 막분리 공정을 조합한 막-생물반응조 공정을 이용하여 염색폐수 중의 난분해성 물질들을 제거하였다. 염색폐수 원수를 직접 막분리공정에 적용한 결과 심각한 fouling을 초래하였다. 반면 생물반응조로 1차 처리한 후 막분리공정에 적용한 경우 생물반응조가 막의 오염원을 상당부분 제거하여 fouling현상이 현저리 감소하였으며, 막의 수명도 연장시킬 수 있었다. 염색폐수의 처리효율 및 fouling현상은 막의 기공 크기나 구조보다는 막의 재질에 더욱 의존함을 확인하였다. 중공사막 module의 사용 방법 및 중공사막 내부의 유속에 따라서 제거효율 및 투과유량이 변화하였다. 생물반응조나 막분리 단독공정보다 두공정을 조합한 공정이 보다 효과적으로 염색폐수 처리능력을 보였다.

• 한국정밀공학회지
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• 제29권9호
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• pp.946-957
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• 2012

The objective of this paper is to investigate into the development of a thin flat panel photo-bioreactor case with characteristics shapes. The thin flat panel photo-bioreactor case was designed to be manufactured from a plastic thermoforming process. A proper design with a relatively high rigidity was obtained through the structural analyses for different designs of the photo-bioreactor case. The thermoforming analyses were performed. From the results of the thermoforming analyses, a proper forming condition and the formability of the designed plastic photo-bioreactor case were estimated. The thermoforming moulds for the flat panel photobioreactor cases were manufactured. The thermoforming experiments were performed to examine the manufacturability of the designed flat panel photo-bioreactor cases. From the results of the thermoforming experiments, it was shown that thin flat panel photo-bioreactor cases with characteristic shapes can be manufactured from the designed thermoforming mould and process.

• 한국물환경학회지
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• 제30권4호
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• pp.386-393
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• 2014

The aim of this study was to investigate the nutrient removal and biomass productivity in the wastewater using MMBR (Microalgae Membrane Bioreactor). MMBR process was combined OPPBR (Optical Panel Photobioreactor) and MBR (Membrane bioreactor). The OPPBR and MBR were operated 3 days and 9h HRT (Hydraulic retention time), respectively, using microalgae as Chlorella vulgaris. The obtained result indicated that the biomass productivity of 0.498 g/L/d with light transmittance of 92% at a 305 mm depth in the OPPBR was achieved. The total consumption of BOD (Biochemical Oxygen Demand) and COD (Chemical Oxygen Demand) in the MMBR were found to be 97.56% and 96.06%, respectively. Additionally, the removal of TN, $NO_3-N$, TP and $PO_4-P$ were 94.94%, 91.04%, 99.54% and 93.06% in MMBR, respectively. These results indicated that the MMBR process was highly effective for COD, BOD and nutrient removal when compared to the separate OPPBR or MBR process. The MMBR process was effective for nutrient removal and biomass productivity and can be applied to treat wastewater in sewage treatment plant.

• KSBB Journal
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• 제6권2호
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• pp.129-133
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• 1991

This study is to investigate the kinetics of anaerobic process, the effect of mass transfer on process, and the characteristics of the conventional anaerobic bioreactor, and develop new high efficiency bioreactor. In the new bioreactor wastewater containing highly concentrated organic materials, was treated without diluting wastewater. In this experiment the high COD removal rate (about 88%) and gas production(about 200l/d) was showed with short residence time(1.5 day). This performance is about 10 times as large as the conventional reactor.

• 한국미생물생명공학회:학술대회논문집
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• 한국미생물생명공학회 2005년도 2005 Annual Meeting & International Symposium
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• pp.233-244
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• 2005

Guanosine fermentation process can be well predicted and analyzed by the proposed state equations describing the dynamic change of a bioreactor. Pyruvate and alanine were found to be characteristically accumulated along with the decline of the guanosine formation rate during the mid-late phase of the process. The enzymological study of the main pathways in glucose catabolism and the quantitative stoichiometric calculation of metabolic flux distribution revealed that it was entirely attributed to the shift of metabolic flux from hexose monophosphate (HMP) pathway to glycolysis pathway. The process optimization by focusing on the restore of the shift of metabolic flux was conducted and the overcoming the decrease of oxygen uptake rate (OUR) was taken as the relevant factor of the trans-scale operation. As a result, the production of guanosinewas increased from 17 g/L to over 34 g/I.

• Journal of Microbiology and Biotechnology
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• 제19권12호
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• pp.1695-1702
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• 2009

An animal-component-free and chemically defined fed-batch process for GS-engineered cell lines producing recombinant antibodies has been developed. The fed-batch process relied on supplying sufficient nutrients to match their consumption, simultaneously minimizing the accumulation of by-products (lactate and osmolality). The proportionalities of nutritional consumption were determined by direct analysis. The robust, metabolically responsive feeding strategy was based on the offline measurement of glucose. The fed-batch process was shown to perform equivalently in GS-CHO and GS-NS0 cultures. Compared with batch cultures, the fed-batch technology generated the greater increase in cell yields (5-fold) and final antibody concentrations (4-8-fold). The majority of the increase in final antibody concentration was a function of the increased cell density and the prolonged culture time. This generic and high-yielding fed-batch process would shorten development time, and ensure process stability, thereby facilitating the manufacture of therapeutic antibodies by GS-engineered cell lines.

• Journal of Microbiology and Biotechnology
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• 제15권1호
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• pp.1-6
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• 2005

Optimal criterion for the scale-up production of schizophyllan, a fungal polysaccharide secreted by Schizophyllum commune, was investigated. For the production of the polysaccharide in a 150-l bioreactor, the culture conditions optimized in a 15-l bioreactor were applied to a 150-l bioreactor with scale-up process, by changing impeller speed and airflow rate. The optimized impeller speed in the 15-l bioreactor was 50 rpm in a technical medium based on barley. For establishment of the scale-up process, 3 kinds of criteria were used while the gas throughput number was kept constant, as follows; constant volume-related power input, constant tip speed of stirrer, and constant Reynolds number. In the 150-l bioreactor, the highest values for the maximum specific growth rate (1.17/day) and productivity (0.63 g/L${\cdot}$day) were achieved in the culture condition from constant volumerelated power input criterion.

• Environmental Engineering Research
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• 제19권3호
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• pp.293-298
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• 2014

An anaerobic photosynthetic treatment process utilizing purple non-sulfur photosynthetic bacteria (PNSB) was applied to the recovery of organic carbon from food processing wastewater. PNSB cells, by-product from the treatment, have high nutrition such as proteins and vitamins which are a good alternative for fish feed. Effects of light source on performance of anaerobic photosynthetic process were investigated in this study. Two bench-scale photo-bioreactors were lighted with infrared light emitting diodes (LEDs) and tungsten lamps covered with infrared transmitting filter, respectively, aiming to supply infrared light for photosynthetic bacteria growth. The photo-bioreactors were operated to treat noodle-processing wastewater for 323 days. Hydraulic retention time (HRT) was set as 6 days. Organic removals in the photo-bioreactor lighted with infrared LEDs (91%-95%) was found higher than those in photo-bioreactor with tungsten lamps with filter (79%-83%). Biomass production in a 150 L bench-scale photo-bioreactor was comparable to a 8 L small-scale photo-bioreactor in previous study, due to improvement of light supply efficiency. Application of infrared LEDs could achieve higher treatment performance with advantages in energy efficiency and wavelength specifity.

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2005년도 생물공학의 동향(XVI)
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• pp.39-39
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• 2005

Large scale mammalian cell culture has become, over the past two decades, the preferred method to produce therapeutic monoclonal antibodies. In this presentation, I will introduce disposable bioreactor system and analyze key factors and points for consideration during mammalian cell culture process development. Example will be provided highlighting the selection of master cell, culture media and environmental factors based on productivity and product quality.

• Transactions on Control, Automation and Systems Engineering
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• 제4권4호
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• pp.277-282
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• 2002

This paper proposes a nonlinear adaptive controller based on back-stepping method for tracking reference substrate concentration by manipulating dilution rate in a continuous baker's yeast cultivating process in stirred tank bioreactor. Control law is obtained from Lyapunov control function to ensure asymptotical stability of the system. The Haldane model for the specific growth rate depending on only substrate concentration is used in this paper. Due to the uncertainty of specific growth rate, it has been modified as a function including the unknown parameter with known bounded values. The substrate concentration in the bioreactor and feed line are measured. The deviation from the reference is observed when the external disturbance such as the change of the feed is introduced to the system. The effectiveness of the proposed controller is shown through simulation results in continuous system.