• Journal of the Korea Academia-Industrial cooperation Society
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• v.18 no.12
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• pp.503-511
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• 2017

This study was conducted to evaluate the anti-inflammatory activity and clinical efficacy of a sample (A.C.C. extracts) obtained by distillation extraction of 14 herbal medicines including Phellodendron bark. To confirm this, the amount of nitric oxide (NO) produced by the cells in RAW 264.7 cells stimulated by lipopolysaccharide (LPS) and the changes in the production of inflammatory cytokines such as tumor necrosis factor (TNF)-${\alpha}$, interleukin(IL)-$1{\beta}$ and IL-6 were determined. The results showed that A.C.C. extracts strongly inhibited the production of NO and inflammatory cytokines increased by LPS without cytotoxicity. In addition, A.C.C. extracts showed strong bacterial reduction rates of 99.9% in Pseudomonas aeruginosa, Staphylococcus aureus, MRSA (Methicillin-resistant Staphylococcus aureus), Candida albicans and Streptococcus mutans. These findings indicate that A.C.C. extracts are effective ingredients with a strong antimicrobial effect together with an anti-inflammatory effect. In addition, when A.C.C. extracts were applied to infants and toddlers who were suffering from diaper rash, itching, and perspiration symptoms, symptoms of rash, atopy, rash, itching, and heat rash were improved. After the lapse of time, it was visually confirmed that it was considerably relaxed. These findings confirm that A.C.C. extracts comprise a clinically effective anti-inflammatory and anti-bacterial agent that alleviates symptoms such as diaper rash and fever and may therefore be an effective alternative to inflammatory diseases.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.21 no.3
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• pp.96-107
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• 2020

This study aimed to identify the associated factors of protective behaviors for radiation exposure among some radiology technologists using the Health Belief Model. The subjects of the study were 541 radiology technologists working at hospitals or clinics in Honam Province. Using the SPSS version 18.0 program, data were analyzed using a t-test, ANOVA, Pearson's correlation analysis, and hierarchical multiple logistic regression analysis. To modify the factors, the performance of subjects who had a higher level of education and nuclear medicine rooms were higher than those who worked in simple radiography rooms. The radiation protective behaviors performance of the subjects who had more exercise, medium-level stress, and worked in higher-quality protection facilities was higher. Regarding the personal perceptions, the cues to action (β=.292, p=.0001), and perceived seriousness (β =.075, p=.010) were factors that had effects on the performance of radiation protection behaviors. Regarding the likelihood of action, the benefits (β=.168, p<.0001), self-efficacy (β=.148, p=.007), and the performance of protective behaviors were higher. In conclusion, protection education as a cue to action should be provided to stimulate protective behaviors, and the benefits of protective behaviors should be emphasized. To increase the performance of protection behaviors, self-efficacy should be enhanced, and the subjects are offered appropriate information that helps perceive seriousness.

• Journal of the Institute of Convergence Signal Processing
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• v.9 no.2
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• pp.104-111
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• 2008

The ECG is biomedical electrical signal occurring on the surface of the body due to the contraction and relaxation of the heart. This signal represents an extremely important measure for health monitoring, as it provides vital information about a patient's cardiac condition and general health. ECG signals are contaminated with high frequency noise such as power line interference, muscle artifact and low frequency nose such as motion artifact. But it is difficult to filter nose from ECG signal, and errors resulting from filtering can distort a ECG signal. The present study implemented a small-size and low-power ECG measurement system that can remove motion artifact for convenient health monitoring during daily life. The implemented ECG monitoring system consists of ECG amplifier, a low power microprocessor, bluetooth module and monitoring program. Amplifier was designed and implemented using low power instrumentation amplifier, and microprocessor was interfaced to the ECG amplifier to collect the data, process, store and feed to a transmitter. And bluetooth module used to wirelessly transmit and receive the vital sign data from the microprocessor to an PC at the receiving site. In order to evaluate the performance of the implemented system, we assessed motion artifact rejection performance in each situation with artificially set condition using adaptive filter.

• The Journal of Korean Medicine
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• v.31 no.1
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• pp.23-29
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• 2010

Objective: The aim of this prospective, case-controlled study was to assess the difference of the amount of tongue coating between halitosis and non-halitosis patients using a digital tongue diagnosis system (DTDS). Methods: Sixty-five patients complaining of oral malodor were recruited for the study. The level of volatile sulfur compounds (VSC) in the oral cavity of the patients was measured by a portable gas chromatography. All patients were then divided into two groups, the halitosis group ($H_2S{\geq}1.5ng$/10ml, or $CH_3SH{\geq}0.5ng$/10ml) and the non-halitosis group ($H_2S$<1.5ng/10ml and $CH_3SH$<0.5ng/10ml), according to the VSC level criteria published by Tonzetich. Tongue images of the two groups were acquired and analyzed by DTDS, followed by the analysis of the tongue coating scores. Results: The tongue coating scores of the halitosis group were significantly higher than of the non-halitosis group (P=0.033). Furthermore, the difference of the posterior tongue coating between the two groups was more significant (P=0.000). Conclusions: Halitosis patients were shown to have a thicker tongue coating than those in the non-halitosis group. Moreover, the posterior dorsum of the tongue should be observed more cautiously when dealing with halitosis patients. Also, the progress and state of the patient's symptom of halitosis as diagnosed objectively by the portable gas chromatography could also possibly be analyzed instead by the DTDS tongue coating score.

• Journal of Korean Ophthalmic Optics Society
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• v.19 no.1
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• pp.99-104
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• 2014

Purpose: This study was to investigate the functional improvement in peripheral vision following eccentric viewing training. Methods: 14 subjects with normal vision took a part with their right eye, peripheral retinal which is $20^{\circ}$ lateral area from the fovea was examined for contrast sensitivity(CS). Eccentric viewing training was performed for 21days with an hour image viewing and examination was repeated. Results: The critical durations for 0.7 cpd were increased 2.67(467 ms) for pre-eccentric viewing training to 2.79(616 ms) for post-eccentric viewing training (p>0.05). The critical durations for 3.0 cpd were also increased 2.53(341 ms) for pre-eccentric viewing training to 3.04(1102 ms) for post-eccentric viewing training (p>0.05). Conclusions: It is recommended to use higher spatial frequency with higher CS for eccentric viewing training and to train more frequently for a short time. Moreover, the study on Korean standardizing of the visual rehabilitation for low vision based on the etiology is sorely required.

• Journal of the Korea Convergence Society
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• v.6 no.5
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• pp.77-84
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• 2015

This study was conducted to investigate the characteristics of antibiotic resistant E. coli. its antibiotic susceptibility and pathogenicity were analyzed via molecular convergence technique, for the relationship of antibiotic susceptibility and pathogenicity. The 60 isolated strains consisted of ESBL(+)(8) and ESBL(-)(52) strains. The ESBL(+)(8) strains consisted of 2 strains without a pathogenic gene, stb(3), flich7(1), and flich7-eae(2). The ESBL(-)(52) strains consisted of 26 strains without a pathogenic gene, stx1(3), stb(10), flich7(2), eae(2), stx1-flich7(2), stx1-stb(4), flich7-stb(2), and flich7-stb-eae(1). In conclusion, antibiotic resistance is increasingly, Focused on molecular convergence, showed the correlation of pathogenicity with antibiotic resistance was poor. However, It will be able to find the exact pathogenic factor in the future through convergence technique including the analysis of virulence genes.

• Journal of Microbiology and Biotechnology
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• v.17 no.4
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• pp.638-643
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• 2007

The full encoding sequence for human type II hexokinase (HXK II) was cloned into the E. coli expression vector pET 21b and expressed as a C-terminally hexahistidine-tagged protein in the BL2l (DE3) strain. The IPTG-induced HXK II approximately accounted for 17% of the total E. coli proteins, and 81% of HXK $II_{6{\times}His}$ existed in inclusion bodies. To improve the production of soluble recombinant HXK II protein, in the functionally active form, we used low temperature, and the osmotic stress expression method. When expressed at $18^{\circ}C$, about 83% of HXK $II_{6{\times}His}$ existed in the soluble fraction, which amounted to a 4.1-fold yield over that expressed at $37^{\circ}C$. The soluble form of HXK $II_{6{\times}His}$ was also highly produced in the presence of 1M sorbitol under the standard condition $(37^{\circ}C)$, which indicated that temperature downshift and low water potentials were required to improve the yield of active recombinant HXK II protein. The expressed protein was purified by metal chelate affinity chromatography performed in an IDA Excellose column charged with $Ni^{2+}$ ions, resulting in about 40mg recombinant HXK II protein obtained with purity over 89% from 51 of E. coli culture. The identity of HXK $II_{6{\times}His}$ was confirmed by Western blotting analysis. Taken together, using the stress-governed expression described in this study, human active HXK II can be purified in sufficient amounts for biochemical and biomedical studies.

• Journal of Microbiology and Biotechnology
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• v.22 no.4
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• pp.555-562
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• 2012

PCR was performed to analyze the ${\beta}$-lactamase genes carried by ampicillin-resistant Vibrio spp. strains isolated from marine environments in Korea between 2006 and 2009. All 36 strains tested showed negative results in PCR with the primers designed from the nucleotide sequences of various known ${\beta}$-lactamase genes. This prompted us to screen new ${\beta}$-lactamase genes. A novel ${\beta}$-lactamase gene was cloned from Vibrio alginolyticus KV3 isolated from the aquaculture water of Geoje Island of Korea. The determined nucleotide sequence (VAK-3 ${\beta}$-lactamase) revealed an open reading frame (ORF) of 852 bp, encoding a protein of 283 amino acids (aa), which displayed low homology to any other ${\beta}$-lactamase genes reported in public databases. The deduced 283 aa sequence of VAK-3, consisting of a 19 aa signal peptide and a 264 aa mature protein, contained highly conserved peptide segments specific to class A ${\beta}$-lactamases including the specific amino acid residues STFK (62-65), SDN (122-124), E (158), and RTG (226-228). Results from PCR performed with primers specific to the VAK-3 ${\beta}$-lactamase gene identified 3 of the 36 isolated strains as V. alginolyticus, Vibrio cholerae, and Photobacterium damselae subsp. damselae, indicating the utilization of various ${\beta}$-lactamase genes including unidentified ones in ampicillin-resistant Vibrio spp. strains from the marine environment. In a mating experiment, none of the isolates transfered the VAK-3 ${\beta}$-lactamase gene to the Escherichia coli recipient. This lack of mobility, and the presence of a chromosomal acyl-CoA flanking sequence upstream of the VAK-3 ${\beta}$-lactamase gene, led to the assumption that the location of this new ${\beta}$-lactamase gene was in the chromosome, rather than the mobile plasmid. Antibiotic susceptibility of VAK-3 ${\beta}$-lactamase was indicated by elevated levels of resistance to penicillins, but not to cephalosporins in the wild type and E. coli harboring recombinant plasmid pKV-3, compared with those of the host strain alone. Phylogenetic analysis showed that VAK-3 ${\beta}$-lactamase is a new and separate member of class A ${\beta}$-lactamases.

• Journal of Biomedical Engineering Research
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• v.23 no.2
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• pp.139-145
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• 2002

Bis-GMA, 2.2-bis[p(2-hydroxy-3-methacryloyloxypropokyl)phenyl]Propane, is an essential component as a multifunctional methacrylate prepolymer in the light-curable polymeric dental composite resins. Two hydroxyl groups of the Bis-GMA molecule are considered to induce water sorption of the photocured composite resin in a mouth, resulting in gradual long-term deterioration of aesthetics and mechanical properties of the composite resins. In this study, some additives such as light stabilizer and antioxidant were added to composite resins to promote durability and storage stability of the last product. First of all, color change increased as a light stabilizer. Tinuvin P, was added to the composed resins and color stability was improved as an antioxidant, Irganox 245, was added to ones. In addition, when Tinuvin P and Irganox 245 were added together to the composed resins. the color stability was enhanced and mechanical properties such as diametral tensile strength before and after acceleration tests were also not greatly decreased. Therefore, when 0.5 weight Percent of Tinuvin P and 0.1 weight percent of Irganox 245 were added together to dental composite resins. the durability and color stability were enhanced, and furthermore the storage stability was also improved for the composed resins.

• Journal of Biomedical Engineering Research
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• v.23 no.6
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• pp.431-436
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• 2002

The purpose of this study was to develop an integration protocol of Nuclear Medicine image with a commercial PACS. Two independent local networks. PACS network and Nuclear Medicine network, were connected using a Nuclear Medicine DICOM gateway A DICOM converter Program was developed to convert Interfile 3.3. which is used in nuclear medicine scanners in our hospital. to DICOM 3.0. The Program converts Interfile format images to those of DICOM format and also transfers converted DICOM files to PACS DICOM gateway. PACS DICOM gateway compares and matches the DICOM image information with patient information in Hospital Information System and then saves to PACS database. The transfer protocol was designed to be able to transfer Interfile. screen dumped file. and also scanned file. We successfully transferred Nuclear Medicine images to PACS. Images transferred by Interfile transfer protocol could be further processed using various tools in PACS. The graphs, numerical information and comments could be conveniently transferred by screen dumped file. The image in a hard copy can be transferred after scanning using an ordinary scanner. The developed protocol can easily transfer Nuclear Medicine images to PACS in various forms with low cost.