• Title/Summary/Keyword: biomass technology

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Estimation of Aboveground Forest Biomass Carbon Stock by Satellite Remote Sensing - A Comparison between k-Nearest Neighbor and Regression Tree Analysis - (위성영상을 활용한 지상부 산림바이오매스 탄소량 추정 - k-Nearest Neighbor 및 Regression Tree Analysis 방법의 비교 분석 -)

  • Jung, Jaehoon;Nguyen, Hieu Cong;Heo, Joon;Kim, Kyoungmin;Im, Jungho
    • Korean Journal of Remote Sensing
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    • v.30 no.5
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    • pp.651-664
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    • 2014
  • Recently, the demands of accurate forest carbon stock estimation and mapping are increasing in Korea. This study investigates the feasibility of two methods, k-Nearest Neighbor (kNN) and Regression Tree Analysis (RTA), for carbon stock estimation of pilot areas, Gongju and Sejong cities. The 3rd and 5th ~ 6th NFI data were collected together with Landsat TM acquired in 1992, 2010 and Aster in 2009. Additionally, various vegetation indices and tasseled cap transformation were created for better estimation. Comparison between two methods was conducted by evaluating carbon statistics and visualizing carbon distributions on the map. The comparisons indicated clear strengths and weaknesses of two methods: kNN method has produced more consistent estimates regardless of types of satellite images, but its carbon maps were somewhat smooth to represent the dense carbon areas, particularly for Aster 2009 case. Meanwhile, RTA method has produced better performance on mean bias results and representation of dense carbon areas, but they were more subject to types of satellite images, representing high variability in spatial patterns of carbon maps. Finally, in order to identify the increases in carbon stock of study area, we created the difference maps by subtracting the 1992 carbon map from the 2009 and 2010 carbon maps. Consequently, it was found that the total carbon stock in Gongju and Sejong cities was drastically increased during that period.

Purification and Characterization of Urushiol Induced Laccase Isoenzyme from Fomitella fraxinea (Urushiol에 의해 유도된 장수버섯 laccase isoenzyme의 정제 및 특성)

  • Choi, Han-Seok;Park, Hyo-Suk;Yeo, Soo-Hwan;Jeong, Seok-Tae;Choi, Ji-Ho;Kim, Myung-Kon
    • The Korean Journal of Mycology
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    • v.38 no.2
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    • pp.152-159
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    • 2010
  • The influence of urushiol, as an allergen on laccase property of Fomitella fraxinea was investigated. The enzyme production was reached to the highest level after 10 days, cultivation and the activity and mycelial biomass were increased by 2.5 and 1.5 folds, respectively, by adding urushiol in the culture medium. In liquid cultures using a Cu Mn-free medium, laccase lactivity was decreased by 3.8-9.2 folds, with similar dry cell weight. Two isoenzymes, were purified using anion exchange, hydrophobic interaction and size-exclusion chromatographies. Both isoenzymes are monomeric proteins, with $M_W$ around 67 kDa(Lac1) and 66 kDa(Lac2), and isoelectric points of 3.67 and 3.81. The optimal conditions for purified isoenzymes were found to be pH 4.5-5.0 and $30-35^{\circ}C$. Activity decreased by the addition of $Fe^{2+}$, $Mg^{2+}$, $Na^+$, and strongly inhibited by EDTA and sodium azide.

Effect of an Endoplasmic Reticulum Retention Signal Tagged to Human Anti-Rabies mAb SO57 on Its Expression in Arabidopsis and Plant Growth

  • Song, Ilchan;Lee, Young Koung;Kim, Jin Wook;Lee, Seung-Won;Park, Se Ra;Lee, Hae Kyung;Oh, Soyeon;Ko, Kinarm;Kim, Mi Kyung;Park, Soon Ju;Kim, Dae Heon;Kim, Moon-Soo;Kim, Do Sun;Ko, Kisung
    • Molecules and Cells
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    • v.44 no.10
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    • pp.770-779
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    • 2021
  • Transgenic Arabidopsis thaliana expressing an anti-rabies monoclonal antibody (mAb), SO57, was obtained using Agrobacterium-mediated floral dip transformation. The endoplasmic reticulum (ER) retention signal Lys-Asp-Glu-Leu (KDEL) was tagged to the C-terminus of the anti-rabies mAb heavy chain to localize the mAb to the ER and enhance its accumulation. When the inaccurately folded proteins accumulated in the ER exceed its storage capacity, it results in stress that can affect plant development and growth. We generated T1 transformants and obtained homozygous T3 seeds from transgenic Arabidopsis to investigate the effect of KDEL on plant growth. The germination rate did not significantly differ between plants expressing mAb SO57 without KDEL (SO plant) and mAb SO57 with KDEL (SOK plant). The primary roots of SOK agar media grown plants were slightly shorter than those of SO plants. Transcriptomic analysis showed that expression of all 11 ER stress-related genes were not significantly changed in SOK plants relative to SO plants. SOK plants showed approximately three-fold higher mAb expression levels than those of SO plants. Consequently, the purified mAb amount per unit of SOK plant biomass was approximately three times higher than that of SO plants. A neutralization assay revealed that both plants exhibited efficient rapid fluorescent focus inhibition test values against the rabies virus relative to commercially available human rabies immunoglobulins. KDEL did not upregulate ER stress-related genes; therefore, the enhanced production of the mAb did not affect plant growth. Thus, KDEL fusion is recommended for enhancing mAb production in plant systems.

Development of CO2 Emission Factor for Wood Chip Fuel and Reduction Effects (목질계 바이오매스 중 대체연료 우드칩의 온실가스(CO2) 배출계수 개발 및 저감 효과)

  • Lee, Seul-Ki;Kim, Seung-Jin;Cho, Chang-Sang;Jeon, Eui-Chan
    • Journal of Climate Change Research
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    • v.3 no.3
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    • pp.211-224
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    • 2012
  • Technology for energy recovery from waste can reduce the greenhouse gas emissions. So recently, there are several companies using RDF, RPF, WCF instead of using only coal fuel and it's part of the fuel on the increase. In this study, we developed Wood chip fuel $CO_2$ emission factor through fuel analysis. It's moisture content is 23%, received net calorific value is 2,845 kcal/kg, and received basis carbon is 34%. The result of emission factor is $105ton\;CO_2/TJ$, it's 5.9% lower than 2006 IPCC guideline default factor $112ton\;CO_2/TJ$. The gross GHG(Greenhouse gases) emissions of plant A is $178,767ton\;CO_2 eq./yr$, and Net GHG emissions is $40,359ton\;CO_2 eq./yr$. Therefore, the reduction of GHG emissions is $138,408ton\;CO_2/yr$ through using WCF, and I accounts for 77% of all GHG emissions.

Corn Growth and Development influenced by Potential CO2 Leakage from Carbon Capture and Storage (CCS) Site (지중저장 이산화탄소의 잠재적 누출 모사에 따른 옥수수 초기 반응 및 생장 연구)

  • Kim, You Jin;Chen, Xuanlin;He, Wenmei;Yoo, Gayoung
    • Journal of Climate Change Research
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    • v.8 no.3
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    • pp.257-264
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    • 2017
  • Carbon capture and storage (CCS) technology has been suggested as an ultimate strategy for mitigating climate change. However, potential leakage of $CO_2$ from the CCS facilities could lead to serious damage to environment. Plants can be a bio-indicator for $CO_2$ leakage as a cost-effective way, although plants' responses vary with plant species. In this study, a greenhouse experiment was conducted to investigate the relation between the $CO_2$ tolerance of corn species and the initial physiological responses to the elevated soil $CO_2$ concentration. Treatment groups included CI (99.99% $CO_2$ gas injection) and BI (no gas injection). Mean soil $CO_2$ concentration for the CI treatment was 19.5~39.4%, and mean $O_2$ concentration was 6.6~18.4%. The soil gas concentrations in the BI treatment were at the ambient levels. In the CI treatment, chlorophyll content was not decreased until the $13^{th}$ day of the $CO_2$ injection. On the $15^{th}$ day, leaf starch content and stomatal conductance were increased by 89% and 25% in the CI treatment compared to the BI treatment, respectively. This might be due to the compensatory reaction of corn to avoid high soil $CO_2$ stress. However, the prolonged $CO_2$ injection decreased chlorophyll content after 13 days. After $CO_2$ injection, plant biomass was reduced by 25% in the CI treatment compared to the BI treatment. Due to the inhibited root growth, leaf phosphorous and potassium contents were decreased by 54% on average in the CI treatment. This study indicates that corn has a high tolerance to soil $CO_2$ concentration of 30% for 2 weeks by its compensatory reactions such as an maintenance of chlorophyll content and an increase in stomatal conductance.

Importance-Performance Analysis of the Livestock Organic Wastes Recycling Policy (축산 유기성 폐기물 자원화 정책의 중요도-만족도 분석)

  • Kim, Won-Tae;Suh, Dae-Seok
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.19 no.12
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    • pp.523-531
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    • 2018
  • The purpose of this paper is to derive priorities and implications for the organic resource conservation policy in the livestock sector. We conducted a survey on the importance-performance of the organic waste resource reclamation of livestock sector using a 5-point Likert scale. The importance average for the resource recycling of livestock organic waste was 3.63 and the average of performance was 3.04. As a result of the IPA on livestock manure recycling measures, it is necessary to improve feed quality, establish a local recycling system, increase demand for compost and liquid, enhance customer linkages, and develop cost reduction technologies. It requires intensive support for promoting the spread of odor reduction technologies and integrated management of biomass. It is necessary to introduce mid- and long-term measures such as the revival of feed in tariff, promote by-product feeding, establish solid fuel process management standards, create hygiene safety standards, develop eco-beads and promotion of feed conversion. It is required to strengthen support for the development of odor reduction technologies and prepare consultative organizations among related departments, develop eco-friendly solid fuel technology, and support policies for renewable energy certification.

Effect of Irrigation of River Water and Swine Slurry Liquid Fertilizer on Kenaf (Hibiscus cannabinus L.) Growth Cultivated Using Soil Moisture Control System in Reclaimed Land (자동 수분 제어시스템을 이용한 간척지 케나프 재배시 하천수 및 액비 관개 효과)

  • Kang, Chan-Ho;Lee, In-Sok;Lee, Jin-Jae;Kim, Hee-Jun
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.66 no.1
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    • pp.87-96
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    • 2021
  • Information and Communication Technology (ICT) remote soil moisture control system including soil sensing, automatic water supply chain, and remote alarming system was established on reclaimed land and operated stably. The system was operated using river water around the reclaimed land without fertilizer. On applying this system to control soil moisture, the kenaf germination rate was improved up to two times. Kenaf biomass was 4,748 kg/10a and was higher than that of untreated soil moisture management. When the nutritious liquid fertilizer was used, kenaf yield reached 8,390 kg/10a, which was lower than 10,848 kg/10a of the non-reclaimed land treated with standard chemical fertilizers. As the soil moisture was managed stably through the ICT remote soil moisture control system, the quality of the kenaf crop was improved, resulting in a 7% increase in dry weight, and a 11.5% increase in plant hardness. The estimated kenaf yield was 5,039 kg/10a when 800 tonnes of water were supplied by the ICT remote soil moisture control system with the stream water around Saemangeum reclaimed land without chemical fertilizers and organic matter.

Comparison of Water Quality and Growth on Leafy Vegetables in Far Eastern Catfish (Silurus asotus) Aquaponics and Hydroponic Culture (메기(Silurus asotus) 양어 순환식 아쿠아포닉스와 수경재배 엽채류의 생육 및 수질의 비교)

  • Min-Jeong Kim;Chang-Ki Shim;One-Seong Park;Jae-Hyeong Lee;Jeong-Wook Heo
    • Korean Journal of Environmental Agriculture
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    • v.42 no.4
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    • pp.408-417
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    • 2023
  • This study aimed to investigate the effects of an aquaponics system utilizing catfish recirculating water on the growth and water quality in lettuce and chicory cultivation. After 60 d of catfish cultivation in the BFT-AP, the survival rate was 96.0% and average weight gain was 178.5 g. The WGR grew by 241.4% and the SGR was 3.0%. A comparison between lettuce and chicory cultivation using BFT-AP and hydroponics revealed significant increases in shoot height, leaf count per week, and biomass of the cultivated lettuce and chicory. The SPAD values also increased significantly by 15.4-43.3%. Water quality analysis of the BFT-AP systems and crop cultivation beds showed significant differences, except for K and Na. The average NO3- concentration in the sediment pond was significantly higher at 45.3±1.0. Comparison of water temperature, pH, EC, DO, and ORP in the BFT-AP system and crop cultivation beds revealed similar monthly water temperatures from July to September. The pH in the catfish culture pond was 0.3-1.2 lower than that in the crop cultivation beds, and the EC was approximately 0.01- 0.03 dS/m lower in the catfish culture pond than the crop cultivation beds. The monthly average ORP in the catfish culture bed steadily increased to 479.9 mV in October. Bacillus velezensis (AFB2-2) and Chlorella fusca (CF) were applied to the biological activation bed; the population density of CF showed a continuous increase, whereas that of AFB2-2 decreased after 15 d and gradually increased thereafter. Thus, the application of the recirculating aquaponics cultivation technology with catfish (BFT) enhances catfish cultivation and promotes the growth of lettuce and chicory. Therefore, it is considered suitable for the environment-friendly cultivation of lettuce and chicory.

Effect of the Various Sources of Dietary Additives on Growth, Body Composition and Shell Color of Abalone Haliotis discus hannai (다양한 원료의 사료첨가제가 전복의 성장, 체조성 및 패각 색채에 미치는 영향)

  • Cho, Sung-Hwoan;Park, Jung-Eun;Kim, Chung-Il;Yoo, Jin-Hyung;Lee, Sang-Min;Choi, Cheol-Young
    • Journal of Aquaculture
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    • v.19 no.4
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    • pp.275-280
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    • 2006
  • Effect of the various sources of dietary additives on growth, body composition and shell color of abalone Haliotis discus hannai was investigated for 16 weeks. Forty juvenile abalone averaging 13.5 g were randomly stocked into 21 of 50 L plastic rectangular containers each. Eight kinds of additives were prepared for this study: four commercially available microalgae [Haeatococcus (Hae), Isochrysis galbana (Iso), Shizochytrium (Sch) and Spirulina (Spi)], three crustacean meals [krill meal (KM), shrimp head meal (Shm) and red crab meal (Rcm)], and green tea by-product (Gre). In addition, dry sea tangle (Dst), Laminaria japonica, as a control, was prepared. Casein, dextrin and a mixture corn oil and fish oil was protein, carbohydrate and lipid sources, respectively, in the experimental diets. The 2% each additive was included into the experimental diets. The experimental diets were fed to abalone once a day at the ratio of $1.5{\sim}2.0%$ total biomass of abalone with a little leftover throughout the 16-week feeding trial. Survival of abalone was not significantly (P>0.05) affected by the experimental diets. However, weight gain of abalone fed the all experimental diets containing the various sources of additives was significantly (P<0.05) higher than that of abalone fed the Dst diet. Weight gain of abalone fed the Spi diet was highest and Shi, KM and Iso diets in order. Shell length and the ratio of soft body weight to body weight of abalone was not significantly (P>0.05) affected by the experimental diets. However, shell width of abalone fed the all experimental diets containing the various sources of additives was significantly (P<0.05) higher than that of abalone fed the Dst diet. The shell color of abalone fed the Spi diet was improved the most distinctively and similar to that of natural abalone. Therefore, it can be concluded that the experimental diets with the various sources of additives (microalgae and crustacean meals) was effective to improve growth of abalone and dietary inclusion of Spirulina was most effective to improve shell color of abalone.

Effect of Composition of γ-Al2O3/SiO2 Mixed Support on Fischer-Tropsch Synthesis with Iron Catalyst (철 기반 촉매의 Fischer-Tropsch 합성에서 γ-Al2O3/SiO2 혼합 지지체 조성의 영향)

  • Min, Seon Ki;No, Seong-Rae;You, Seong-sik
    • Korean Chemical Engineering Research
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    • v.55 no.3
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    • pp.436-442
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    • 2017
  • Fischer-Tropsch synthesis is the technology of converting a syngas (CO+$H_2$) derived from such as coal, natural gas and biomass into a hydrocarbon using a catalyst. The catalyst used in the Fischer-Tropsch synthesis consists of active metal, promoter and support. The types of these components and composition affect the reaction activity and product selectivity. In this study, we manufactured an iron catalyst using ${\gamma}-Al_2O_3/SiO_2$ mixed support (100/0 wt%, 75/25 wt%, 50/50 wt%, 25/75 wt%, 0/100 wt%) by an impregnation method to investigate how the composition of ${\gamma}-Al_2O_3/SiO_2$ mixed support effects on the reaction activity and product selectivity. The physical properties of catalyst were analyzed by $N_2$ physical adsorption and X-Ray diffraction method. The Fischer-Tropsch synthesis was conducted at $300^{\circ}C$, 20bar in a fixed bed reactor for 60h. According to the results of the $N_2$ physical adsorption analysis, the BET surface area decreases as the composition of ${\gamma}-Al_2O_3$ decreases, and the pore volume and pore average diameter increase as the composition of ${\gamma}-Al_2O_3$ decreases except for the composition of ${\gamma}-Al_2O_3/SiO_2$ of 50/50 wt%. By the results of the X-Ray diffraction analysis, the particle size of ${\alpha}-Fe_2O_3$ decreases as the composition of ${\gamma}-Al_2O_3$ decreases. As a result of the Fischer-Tropsch synthesis, the CO conversion decreases as the composition of ${\gamma}-Al_2O_3$ decreases, and the selectivity of C1-C4 decreases until the composition of ${\gamma}-Al_2O_3$ was 25 wt%. In contrast, the selectivity of C5+ increases until the composition of ${\gamma}-Al_2O_3$ is 25 wt%.