• Title/Summary/Keyword: biological production facility

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Enhanced Biomass and ${\gamma}$-Linolenic Acid Production of Mutant Strain Arthrospira platensis

  • Choi, Gang-Guk;Bae, Myong-Sook;Ahn, Chi-Yong;Oh, Hee-Mock
    • Journal of Microbiology and Biotechnology
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    • v.18 no.3
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    • pp.539-544
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    • 2008
  • A mutant of Arthrospira platensis PCC 9108, strain M9108, obtained by mutagenesis with UV treatment, was able to mixotrophically grow in an SOT medium containing 40 g of glucose/l. The biomass and specific growth rate of strain M9108 (4.10 g/l and 0.70/d) were 1.9-fold and 1.4-fold higher, respectively, than those of the wild type (2.21 g/l and 0.58/d) under mixotrophic culture condition. In addition, when compared with the wild type, the content of ${\gamma}$-linolenic acid (GLA) in the mutant was increased when glucose concentration was increased. Compared with the wild type, the GLA content of the mutant was 2-fold higher in autotrophic culture and about 3-fold higher in mixotrophic culture. Thus, the mutant appears to possess more efficient facility to assimilate and metabolize glucose and to produce more GLA than its wild-type strain.

Growth and r-Linolenic Acid Production of Arthrospira (Spirulina) platensis in Heterotrophic Culture. (Arthrospira (Spirulina) platensis의 종속영양배양과 r-Linolenic Acid 생산)

  • Choi, Gang-Guk;Bae, Myoung-Sook;Park, Je-Seop;Park, Bok-Jun;Ahn, Chi-Yong;Oh, Hee-Mock
    • Microbiology and Biotechnology Letters
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    • v.35 no.1
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    • pp.45-51
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    • 2007
  • Arthrospira (Spirulina) platensis is one of the commercially important filamentous cyanobacteria. The heterotrophic cultivation of Arthrospira can be an alternative strategy for commercial mass production. In heterotrophic culture, the specific growth rate of A. platensis M9108, a glucose-resistant mutant of A. platensis PCC 9108, was $0.014h^{-1}$ which was 1.8 higher than that oi the previous report. The mutant possessed the facility to assimilate and to metabolize glucose efficiently under heterotrophic condition. However, the r-linolenic acid content of 6 Arthrospira strains was not increased in heterotrophic culture. Four Arthrospira strains out of 6 tested strains were able to utilize maltose as a carbon source under heterotrophic condition. The biomass production of these strains on maltose was similar to that on glucose. The specific growth rate of A. platensis M9108 increased with glucose concentration up to 5.0 g/L and then decreased at a glucose concentration of 10.0 g/L. Additionally, A. platensis M9108 under heterotrophic condition showed no aggregation during the cultivation in contrast to A. platensis PCC 9108.

Studies on the Structural Design of Biological Production Facility (2) A Simple Method for Design Load Estimation and Safety Test (생물생산시설의 구조설계에 관한 연구 (2) 설계하중 산정 및 안전성 검토를 위한 간이적 방법 개발)

  • 김문기;손정익;남상운
    • Proceedings of the Korean Society for Bio-Environment Control Conference
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    • 1992.12a
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    • pp.31-32
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    • 1992
  • 일반적으로 시설을 설계함에 있어서 고정하중(자중)은 부재단면이 결정되기 전이므로 추정에 의한 값을 사용한다. 그러나 생물생산시설에 관한 기준이 마련되어 있지 않기 때문에 국내 실정에 맞는 자료가 요청된다. 또한 구조설계 과정은 복잡하고 전문적 지식을 요하므로 전문가에 의해서 수행된다. 그러나 생물생산시설의 규모나 형태는 비슷한 경우가 많으므로 대표적인 몇가지 시설에 대하여 구조해석을 실시하고, 이것을 일반화함으로써 비전문가인 농민이나 생물생산분야 종사자가 간편하게 시설의 구조적 안전성을 진단할 수 있을 것으로 생각한다.(중략)

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The Status of the KRR-l&2 Decommissioning Activities

  • Chung, Un-Soo;Park, Seung-Kook;Hong, Sang-Bum;Park, Jin-Ho
    • Proceedings of the Korean Radioactive Waste Society Conference
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    • 2004.02a
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    • pp.96-105
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    • 2004
  • The decommissioning project of the KRR 1 & 2 was started in January 1997. The actual decommissioning activity was started at the RI production facility and was finished at the end of 2002. The dismantling works of all components including the reactor structure of the KRR-2 was started in January, 2003 and will be carried out for 2 years till the end of 2004. The project schedule is estimated to delay for 4∼5 months beyond the original plan because of delaying on the cutting of thermal column nose and removal of the graphite bricks, but it may be caught up during the removal working of concrete from biological shielding structure. This paper summarizes the general status of the KRR 1 & 2 and decommissioning activities.

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Design of waste Sludge/Food Waste Biological Treatment Process using Closed ATAD System (밀폐형 ATAD system을 이용한 하수슬러지/음식물쓰레기 통합처리 공정 설계)

  • Kwon, Hyeok-Young;Ji, Young-Hwan;Song, Han-Jo;Kim, Seong-Jung
    • Journal of the Korea Organic Resources Recycling Association
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    • v.8 no.4
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    • pp.129-137
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    • 2000
  • In this study, biological treatment process of MWWT(Municipal wet-waste Treatment) has been developed through a moduling of the containerized closed ATAD(Auto thermal aerobic digestion) system & closed vertical dynamic acerator, which were used for food waste and cattle manure, respectively. Though biological process has several advantages such as low concentrations of heavy metals and salts, proper and stable C/N ratio and constant reaction rate against the process treating two wastes separately, it has a obstacles of salt concentration and much usage of bulking agent such as wood chip. After rapid oxidation in the boxed tower reactor for 5 days, the content of sewage sludge would be reduced 65% on around, might be mixed with the food waste that had been treated in the static closed reactor during 6 days and put in the secondary static reactor for curing. During composting process, the odor contained in the gas generated from the reactor was removed by passing it through a biofilter as well as the leachate was treated in the wastewater treatment facility. Consequently, it seemed to be possible to compost sewage sludge at mild and stable operating condition and at low cost through the biological ATAD process resulting in the production of organic compost satisfying the specifications regulated by itself.

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Body Weight Changes of Laboratory Animals during Transportation

  • Lee, Sung-Hak;Nam, Hyun-Sik;Kim, Jin-Sung;Cho, Hye-Jung;Jang, Yu-Mi;Lee, Eun-Jung;Choi, Eun-Sung;Jin, Dong-Il;Moon, Hong-Sik
    • Asian-Australasian Journal of Animal Sciences
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    • v.25 no.2
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    • pp.286-290
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    • 2012
  • The majority of laboratory animals were transported from commercial breeders to a research facility by ground transportation. During the transportation, many biological functions and systems can be affected by stress. In this experiment, the change of body weight during the transportation was measured and the recovery periods from the transportation stress established based on the body weight changes. Total 676 laboratory animals which were aged between 3 to 9 wk old were studied. The transportation time taken from container packing to unpacking the container was approximately 24 h. The temperature of animal container was constantly maintained by air-conditioning and heating equipment. Rats were found to be more sensitive than mice. The body weight of rats was significantly decreased 3.71% (p<0.05) compared to the body weight of mice which decreased 0.9% There was no significant difference between the strains in the same species. When the changes of body weights were compared between delivery days, C57BL/6 mice showed the most variable changes compared to other species and strains. Consequently, C57BL/6 was more sensitive to stress than the other strains and the transportation process needs to be standardized to reduce between day variability. To establish the recovery periods from transportation stress, the body weight changes were measured during the acclimation period. Although the body weight of animals decreased during transportation, animals recovered their weight loss after the next day.

A Study on Improvement of Distribution Facility in Wholesale Agricultural Products Market

  • Gal, Won-Mo;Kwon, Ki-Tae;Lee, Woo-Sik;Choi, Eun-Mee;Kwon, Lee-Seung;Seong, Seung-Hwan;Kwon, Woo-Taeg
    • Journal of Distribution Science
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    • v.16 no.2
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    • pp.53-65
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    • 2018
  • Purpose - The purpose of this study is to investigate the effect of gamma - irradiation on the effluent from food distribution in the large agricultural and marine products market. This study will contribute to the distribution process as well as the agricultural and fishery distribution facilities. Research design, data, and methodology - In order to reduce the odor, the smell was examined in the anaerobic digestion process by irradiating gamma rays to the wastewater of mixed food discharged from a large restaurant. An odor determination panel list was constructed to determine if the odor was present in the air dilution drainage and the odor concentration was analyzed by instrumental analysis. Results - It was confirmed that the sulfur content increased gradually from 3 months. Ammonia decreased from 33.57ppm at the initial measurement to 4.12 ppm at the end of the experiment. Methane production was highest at 100kGy when exposed to gamma rays of 0-200kGy at pH 12. In other words, it is considered that gamma irradiation doses are most effective at 100kGy and are suitable for large capacity wastewater treatment facilities in terms of economic feasibility. Conclusions - In pre-treatment of compound food wastewater, gamma irradiation is most cost effective when examined at 100kGy. The economic efficiency of the pre-treatment method by gamma irradiation is much higher than the wastewater treatment in the large-scale agricultural and marine products distribution market.

Effect of aerobically treated manure on odorous material emissions from a swine finishing barn equipped with a continuous pit recirculation system

  • Choi, Yongjun;Ha, Duck-Min;Lee, Sangrak;Kim, Doo-Hwan
    • Animal Bioscience
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    • v.35 no.2
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    • pp.308-316
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    • 2022
  • Objective: This study was conducted to determine reduction of various odorous materials from a swine farm equipped with a continuous pit recirculation system (CPRS) with aerobically treated liquid manure. Methods: The CPRS is used in swine farms in South Korea, primarily to improve air quality in pig houses. In this study, CPRS consists of a manure aerobic treatment system and a fit recirculation system; the solid fraction is separated and composted, whereas the aerobically treated liquid fraction (290.0%±21.0% per day of total stored swine slurry) is continuously returned to the pit. Four confinement pig barns in three piggery farms were used; two were equipped with CPRS and the other two operated a slurry pit under the slatted floor. Results: All chemical contents of slurry pit manure in the control were greater than those of slurry pit manure in the CRPS treatment (p<0.05). Electrical conductivity and pH contents did not differ among treatments. The biological oxygen demand of the slurry pit treatment was greater than that of the other treatments (p<0.05). Total nitrogen, total phosphorus, and ammonia nitrogen contents of the slurry pit treatment were greater than those of other treatments (p<0.05). Odor intensity of the CPRS treatment was lower than that of the control at indoor, exhaust, and outside sampling points (p<0.05). The temperature and carbon dioxide of the CPRS treatment in the pig barn was significantly lower than those of control (p<0.05). All measured odorous material contents of the CPRS group were significantly lower than those of the control group (p<0.05). Conclusion: The CPRS application in pig farms is considered a good option as it continuously reduces the organic load of animal manure and lowers the average odorant concentration below the threshold of detecting odorous materials.

Appropriate temperature of antler-shaped Ganoderma lucidum using King Oyster Mushroom Cultivation System (큰느타리(King Oyster Mushroom) 재배시스템을 활용한 녹각영지버섯(Ganoderma lucidum) 적정 재배온도)

  • Jo, Woo-Sik;Kim, Minkyeong;Hwang, Jae Soon
    • Journal of Mushroom
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    • v.20 no.3
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    • pp.183-185
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    • 2022
  • High-income mushroom crops require complex farming. Therefore, we conducted a test to identify the optimum temperature for the production of antler-shaped Ganoderma lucidum using the King Oyster mushroom cultivation facility. T-N showed 0.28% of oak sawdust and 2.2% of nutritional source. The pH of oak sawdust was 6.0, indicating weak acidity, and that of rice steel was 6.6, indicating neutrality in nutrition source. Study on the quality characteristics of mushrooms showed that the number of days at 25℃ were 5~6 and those at 30℃ were 3~5; the representative length at 25℃ was 57.5 mm and that at 30℃ was 92.2 mm; the biological weight at 25 ℃ was 43 g, which was greater than that at 30℃.

Survey of Physicochemical Methods and Economic Analysis of Domestic Wastewater Treatment Plant for Advanced Treatment of Phosphorus Removal (총인 수질기준강화를 위한 국내 하수종말처리장의 물리화학적처리 특성조사 및 경제성 분석)

  • Park, Hye-Young;Park, Sang-Min;Lee, Ki-Cheol;Kwon, Oh-Sang;Yu, Soon-Ju;Kim, Shin-Jo
    • Journal of Korean Society of Environmental Engineers
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    • v.33 no.3
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    • pp.212-221
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    • 2011
  • Wastewater treatment plants (WWTPs) are required to meet the reinforced discharge standards which are differentiated as 0.2, 0.3 and 0.5 mg-TP/L for the district I, II and III, respectively. Although most of WWTPs are operating advanced biological phosphorus removal system, the supplementary phosphorus treatment facility using chemical addition should be required almost at all WWTPs. Therefore, water quality data from several exemplary full-scale plants operating phosphorus treatment process were analyzed to evaluate the reliability of removal performance. Additionally, a series of jar tests were conducted to find optimal coagulants dose for phosphorus removal by chemical precipitation and to describe characteristics of the reaction and sludge production. Chemical costs and the increasing sludge volume in physicochemical phosphorus removal process were estimated based on the results of jar tests. The minimum coagulant (aluminium sulfate and poly aluminium chloride) doses to keep TP concentration below 0.5 and 0.2 mg/L were around 25 and 30 mg/L (as $Al_2O_3$), respectively, in the mixed liquor of activated sludge. In the tertiary treatment facility, relatively lower coagulant doses of 1/12~1/3 the minimum doses for activated sludge were required to achieve the same TP concentrations of 0.2~0.5 mg/L. Increase in suspended solids concentration due to chemical precipitates in mixed liquor was estimated at 10~11%, compared to the concentration without chemical addition. When coagulant was added into mixed liquor, chemical (aluminium sulfate) cost was estimated to be 4~10 times higher than in secondary effluent coagulation/separation process. Sludge production to be wasted was also 4~10 times higher than secondary effluent coagulation/separation process.