• 한국환경성돌연변이발암원학회지
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• 제21권2호
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• pp.164-168
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• 2001

Abel et al. in Germany discovered a new dioxin-responsive gene, which has later been identified as rpt-1 (regulatory protein T-lymphocyte 1). While it is speculated that rpt-1 may play a role in signal transduction and carcinogenesis, its roles and functions remain unknown. The present study attempted to analyze functions of rpt-1 in human epithelial cells following the xenobiotic exposures. While German counterpart analyzed expressionn of rpt-1 in spleen and thymus cells from mouse and rat and characterizes molecular properties of the gene, our work mainly focused on analyzing function of rpt-1 in human skin cells. Expression of rpt-1 in human cells were analyzed by western and northern blot RT-PCR analysis. Expression of rpt-1 as well as Staf-50 in human cells with or without exposure to environmental pollutants were also analyzed by northern blot analysis, since Staf-50 is homologous with rpt-1 and found in human cells. To help study roles of rpt-1 in human cell system, retroviral vector system carrying rpt-1 gene under the CMV promoter were constructed and transfected. Cells overexpressing the gene after the transfection showed an increase of cell density and soft agar colony formations, as compared to the control cells, suggesting that rpt-1 may play a certain role in the transformation processes of human cells. While the expression of rpt-1 in spleen and thymus is known to be strong in the laboratory animals, both the basal and TCDD-induced expression of rpt-1 in the current cellular system remained insignificant. It is speculated that the expression pattern of rpt-1 may be tissue- and species-specific. The present study demonstrated a strong expression of rpt-1 protein in the brain of SD rat model. Since there is no previous report on the expression of rpt-1 in the brain tissue, the result may play a significant role in understanding dioxin-induced neurotoxicities in the future. The present study provides an opportunity to understand a role of rpt-1 in human cell system and suggest a possible lead and basis for the future study of dioxin-induced neurotoxicities.

• 한국산학기술학회논문지
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• 제7권3호
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• pp.440-447
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• 2006

본 연구의 목적은 미나리와 끈상 미생물접촉재를 이용하여 생활하수의 하수처리장 방류수의 수질 개선을 위함이다. 최종 방류수의 수질을 개선하기 위한 침전조 모형 플랜트를 2개 제작하여 실험하였는데, 하나(Tank 1)는 기존 하수처리장 침전조와 같게 제작 하였으며, 다른 하나의 침전조(Tank 2)는 침전조의 수초섬 상부에는 미나리를 식재하고 하부에는 끈상 미생물 접촉재를 배치하였다. 유기 오염물질인 BOD, 부유고형물질인 SS, 질소 그리고 인에 대한 고도처리 효율을 검증하였다. 실험 결과 Tank 2에서 유출되는 배출수가 Tank 1에서 배출되는 유출수보다 처리 효율이 향상되었다. 처리 효율을 살펴보면, Tank 1보다 Tank 2 유출수의 BOD는 $7.9%{\sim}38.8%$가 향상되었으며, 부유고형 물질인 SS는 $14.3%{\sim}60.0%$ 향상되었다. 부영양화 물질인 T-N은 $9.6%{\sim}31.5%$ 향상되었으며, T-P는 $33.0%{\sim}80.0%$까지 향상되었다. 처리 효율로 판단하면 T-P와 SS 제거효율이 BOD와 T-N보다 높았다.

• 상하수도학회지
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• 제23권5호
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• pp.599-608
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• 2009

This manuscript covers the results of field investigation and lab-scale experiments to design a double-layered biofilter system to control urban storm runoff. The biofilter system consisted of a coarse soil layer (CSL) for filtration and fine soil layer (FSL) for adsorption and biological degradation. The variations of flow rate and water quality of runoff from a local expressway were monitored for seven storm events. Laboratory column experiments were performed using seven kinds of soil and mulch to maximize pollutants removal. The site mean concentration (SMC) of storm runoff from the drainage area (runoff coefficient: 0.92) was measured to be 203 mg/L for SS, 307 mg/L for $TCOD_{Cr}$, 12.3 mg/L for TN, 7.3 mg/L for ${NH_4}^+-N$, and 0.79 mg/L for TP, respectively. This study employed a new design concept, to cover the maximum rainfall intensity with one month recurrence interval. Effective storms for last ten years (1998-2007) in seoul suggested the design rainfull intensity to be 8.8 mm/hr Single layer soil column showed the maximum removal rate of pollutants load when the uniformity coefficient of CSL was 1.58 and the silt/clay contents of FSL was virtually 7%. The removal efficiency during operation of double layer soil column was 98% for SS and turbidity, 75% for TCODCr, 56% for ${NH_4}^+-N$, 87% for TP, and 73-91% for heavy metals. The hydraulic conductivity of the soil column, 0.023 cm/sec, suggested that the surface area of the biofilter system should be about 1% of the drainage area to treat the rainfall intensity of one month recurrence interval.

• 보존과학회지
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• 제29권2호
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• pp.93-102
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• 2013

속초 신흥사 목조지장보살삼존상(보물 제1749호)은 신흥사 명부전에 안치되어 있다. 이 삼존상에는 부분적으로 균열, 박락 등의 물리적 손상과 안료 변색, 균류 서식 등의 화학적 및 생물학적 손상이 발생하였다. 이 연구에서는 삼존상의 손상도 평가 및 보존계획을 수립하기 위해 손상지도 작성, 초음파 측정, X-선 및 내시경 촬영 등의 비파괴 정밀진단을 수행하였다. 이 결과, 지장보살에서는 개금층 박락이 관찰되며 각종 오염물이 불상표면에 고착되어 있다. 무독귀왕은 누수에 의한 안료변색이 심하다. 도명존자는 노출된 목부재에서 곤충의 서식흔적이 확인되었으며, 내부에서는 벌집이 관찰되었다. 따라서 이 손상도 진단 결과를 바탕으로 삼존상의 원형을 보존하기 위한 보수가 필요한 것으로 판단된다.

• Environmental Analysis Health and Toxicology
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• 제11권1_2호
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• pp.31-39
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• 1996

Leachate from municipal solid waste (MSW) landfill, effluent from leachate treatment plant, and ground water sample from a monitoring well near landfill site were tested for an acute toxicity. Microtox toxicity test was used for testing the acute toxicity of leachate and other samples. EC$_{50}$ values which a concentration of pollutant for reducing 50% light output from luminescent bacteria, Photobacterium phosphoreum were determined to assess the toxicity of pollutants as well as the relative toxicity. In addition, characteristics of leachate were studied and compared to those of phenol and pentachlorophenol (PCP) which are typical aquatic toxic pollutants. For leachate, EC$_{50}$ for 30 min incubation was 10.8%, while for phenol and PCP, 46 ppm and 1.2 ppm, respectively. the relative toxicity of treated leachate by in situ aeration with activated sludge was reduced to more than 75% of toxicity of the untreated leachate. Microtox toxicity test was failed to figure out EC$_{50}$ values for groundwater from a monitoring well since the relative toxicity of the unconcentrated sample was too low to estimate EC$_{50}$. Addition of activated carbon to leachate was reduced the relative toxicity. The reduction Pattern of the relative toxicity of leachate by mechanical aeration was similar to that of PCP, but different from that of phenol. These findings suggest that the toxicity of leachate may come from PCP-like toxic compounds rather than phenol-like one. In conclusion, the process of aeration with activated sludge might be very important to reduce the environmental toxicity of leachate. And Microtox test could be a reasonable bioassay for screening and monitoring the environmental toxicity of leachate from municipal solid waste landfill as well as for determining the reduction efficiency of the leachate toxicity by various treatment processes in leachate treatment plant.

• Environmental Engineering Research
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• 제22권2호
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• pp.203-209
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• 2017

Innovations in the biofiltration process can provide effective solutions to overcome crucial water pollution problems. The elimination of pollutants is a result of the combined effects of biological oxidation, adsorption and filtration processes. This research aims to evaluate the performance of quartz sand biofiltration for removing total suspended solids, turbidity, color, organic matter, and ammonium from polluted river water and develop an empirical model for designing quartz sand biofilters for the treatment of polluted river water. Experiments were conducted using two biofilter units filled with quartz sand as filter media. A set of experiments were performed to evaluate the effect of hydraulic retention time on biofilter performance in removing water contaminants. The kinetics of organic matter removal were also determined to describe the performance of the biofilter. The results show that biofiltration can significantly remove river water pollutants. Removal efficiency depends on the applied hydraulic retention time. At a hydraulic retention time of two hours, removal efficiencies of total organics, ammonium and total suspended solids were up to 78%, 82%, and 91%, respectively. A model for designing quartz sand biofiltration has been developed from the experimental data.

• 한국환경과학회지
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• 제14권8호
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• pp.777-783
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• 2005

For the sustainable management of marine ecosystem in Masan Bay, we have to assess the carrying capacity and standard of target water quality. In this research, we assume that all pollutants loads are treated in Dukdong sewage treatment plant, then we simulate the physical-biological model for prediction water quality for the achievement of standard water quality. In 2001 year, for the achievement of COD 2.5 mg/L, we need to reduce COD $90\%$, nitrogen $30\%$, phosphate $90\%$ than that of the present value, According to these results, the water quality of sewage treatment plant is required to treat COD 13.5 mg/L, nitrogen 33.3 mg/L, phosphate 6,0 mg/L. If the sewage treatment plant will be expanded much larger in 2011, it will need to be treated in COD 6.6 mg/L, nitrogen 2.5 mg/L, phosphate 5 mg/L for the achievement of water quality standard in COD 2.5 mg/L.

• 한국환경과학회지
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• 제14권6호
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• pp.583-596
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• 2005

Samples of water, soil, and sediment were taken from 10 streams of Andong city in Gyeongbuk province in October 2004. To assess the degree of environmental pollution for each stream site, the chemical analyses of pollutants such as T-N, T-P, COD, heavy metal, organophosphorous and organochlorine pesticides, and dioxin-like PCB congeners were implemented using the standard process tests or the U. S. EPA methods. In addition, biological assessment using insect immune biomarkers was conducted on the same environmental samples to complement the chemical assessment. Except Waya stream (T-N; 2.91 mg/L, T-P; 0.16 mg/L, COD; 14.0 mg/L) with above the environmental quality standards, the T-P and COD concentrations of 9 sites are relatively low. The contents of Pb and Cd in samples taken from each stream were much lower than environmental quality standards. However, in comparison with soil samples of other streams, several times higher concentrations of Pb and Cd were found in locations at Mi, Gilan, Yeonha, and Waya stream sites. Dementon-S-methyl, diazinon, parathion, and phenthoate compounds among organophosphorous pesticides were detected as concentrations of ppb levels, respectively, from soil samples collected in the vicinity of Gilan, Mi, Norim, and Waya stream. On the other hand, 16 organochlorine pesticides and 12 dioxin-like PCB congeners selected in this study were not found in all samples. In particular, considering significant disrupting effects of Waya stream's samples on insect immune capacity, this stream seems to be contaminated with investigated and/or univestigated pollutants in this study.

• 한국농공학회논문집
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• 제64권6호
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• pp.1-12
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• 2022

Estuary reservoirs were artificial reservoir with seawalls built at the exit points of rivers. Although many water resources can be saved, it is difficult to manage due to the large influx of pollutants. To manage this, it is necessary to analyze watersheds and reservoirs through accurate modeling. Therefore, in this study, we linked the Hydrological Simulation Program-FORTRAN (HSPF), Environmental Fluid Dynamics Code (EFDC), and Water quality Analysis Simulation Program (WASP) models to simulate the hydrology and water quality of the watershed and the water level and quality of estuary lakes. As a result of applying the linked model in stream, R² 0.7 or more was satisfied for the watershed runoff except for one point. In addition, the water quality satisfies all within 15% of PBIAS. In reservoir, R² 0.72 was satisfied for water level and the water quality was within 15% of T-N and T-P. Through the modeling system, We applied upstream pollutant management scenarios to analyze changes in water quality in estuary reservoirs. Three pollution source management were applied as scenarios, the improvement of effluent water quality from the sewage treatment plant and the livestock waste treatment plant was effective in improving the quality of the reservoir water, while the artificial wetland had little effect. Water quality improvement was confirmed as a measure against upstream pollutants, but it was insufficient to achieve agricultural water quality, so additional reservoir management is required.

• 환경생물
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• 제38권1호
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• pp.82-92
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• 2020

축산 폐수는 고농도의 영양염류와 중금속을 함유하고 있어, 배출될 때 수질을 악화시킨다. 기존 처리 기술과 비교하여 bioremediation은 축산 폐수 처리에 유능하다. 특히, 미세조류는 오염물질 제거에 잠재력을 가지고 있다. 본 연구에서는 Ankistrodesmus bibraianus를 이용하여 축산 폐수 내 영양염류(질소(N), 인(P))와 중금속(구리(Cu), 아연(Zn))의 제거 가능성을 평가하고, A. bibraianus의 최적 배양조건을 확립하였다. 연구결과, 최적 배양조건은 28℃, pH 7, 광주기는 14:10 h로 설정되었다. N과 P의 단일 처리구(500, 1,000, 5,000, 10,000mg L^-1)에서 제거효율은 각각 22.9~80.6%와 11.9~50.0%였다. 또한, N과 P의 복합 처리구에서 제거효율은 각각 16.4~58.3%와 7.80~49.8%였다. Cu와 Zn의 단일 처리구(10, 30, 50mg L^-1)에서 제거효율은 각각 15.5~81.5%와 6.28~34.3%였다. 유사하게, Cu와 Zn의 복합 처리구에서 제거효율은 각각 16.7~74.5%와 5.58~27.5%였다. 또한 영양염류(N 및 P)와 중금속(Cu 및 Zn)의 성장 및 제거효율을 축산 폐수에 적용할 수 있음을 나타냈다. 본 연구의 결과에 따르면 A. bibraianus는 축산 폐수 내 영양염류와 중금속 제거에 이용할 수 있을 것으로 사료된다.