• Journal of Microbiology and Biotechnology
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• 제31권1호
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• pp.115-122
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• 2021

Phenol-soluble modulins (PSMs) are responsible for regulating biofilm formation, persister cell formation, pmtR expression, host cell lysis, and anti-bacterial effects. To determine the effect of psm deletion on methicillin-resistant Staphylococcus aureus, we investigated psm deletion mutants including Δpsmα, Δpsmβ, and Δpsmαβ. These mutants exhibited increased β-lactam antibiotic resistance to ampicillin and oxacillin that was shown to be caused by increased N-acetylmannosamine kinase (nanK) mRNA expression, which regulates persister cell formation, leading to changes in the pattern of phospholipid fatty acids resulting in increased anteiso-C15:0, and increased membrane hydrophobicity with the deletion of PSMs. When synthetic PSMs were applied to Δpsmα and Δpsmβ mutants, treatment of Δpsmα with PSMα1-4 and Δpsmβ with PSMβ1-2 restored the sensitivity to oxacillin and slightly reduced the biofilm formation. Addition of a single fragment showed that α1, α2, α3, and β2 had an inhibiting effect on biofilms in Δpsmα; however, β1 showed an enhancing effect on biofilms in Δpsmβ. This study demonstrates a possible reason for the increased antibiotic resistance in psm mutants and the effect of PSMs on biofilm formation.

• 대한예방한의학회지
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• 제3권2호
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• pp.91-100
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• 1999

Today, toxicology is used for many purpose, in many fields. Classification of special toxic effect is related next 4 important principles. 1. The chemical substance must move to target organ or tissue that can induce Biological effect. For this movement, we have to understand the physical-chemical characteristic of substance, and the rout of absorption, metabolism, diffusion and excretion of toxic substance. 2. Every biological effect that induced by chemical substance is not harmful. For example, some specific chemical substance is not harmful in liver enzyme system. 3. The strength of biological effect induced by chemical substance is deep related with dose. Nearly all substance is not effective below the specific dose, and it may toxic to death over the specific dose. It is the 'Dose - response relationship' But carcinogen may toxic whether it is law dose or not. 4. The information that was obtained by experimental animal test, could have to adapt in human biology. Because biological effect of chemical substance could be different in every biological species. In past, drugs was obtained by animal or plants. But in the future, it could be obtained by biochemistry, and genome project. Therefore, in Oriental medicine, research and approach is needed at this time, and have to develop new method of experience in toxic method.

• 한국토양비료학회지
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• 제48권2호
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• pp.146-152
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• 2015

Heavy metal pollution has been a critical problem in agricultural field near at the abandoned metal mines and chemical amendments are applied for remediation purpose. However, biological activity can be changed depending on chemical amendments affecting crop productivity. Main purpose of this research was to evaluate biological parameters after applying chemical amendments in heavy metal polluted agricultural field. Result showed that soil respiration (SR) and microbial biomass carbon (MBC) were changed after chemical amendments were applied. Among three different amendments, lime stone (LS), steel slag (SS), and acid mine drainage sludge(AMDS), AMDS had an effect to increase SR in paddy soil. Comparing to control ($93.98-170.33mg\;kg^{-1}day^{-1}$), average of 30% increased SR was observed. In terms of MBC, SS had an increased effect in paddy soil. However, no significant difference of SR and MBC was observed in upland soil after chemical amendment application. Overall, SR can be used as an indicator of heavy metal remediation in paddy soil.

• 한국약용작물학회:학술대회논문집
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• 한국약용작물학회 2012년도 심포지엄 및 춘계학술발표회
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• pp.291-292
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• 2012

• Molecules and Cells
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• 제26권2호
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• pp.121-130
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• 2008

Methamphetamine, a commonly used addictive drug, is a powerful addictive stimulant that dramatically affects the CNS. Repeated METH administration leads to a rewarding effect in a state of addiction that includes sensitization, dependence, and other phenomena. It is well known that susceptibility to the development of addiction is influenced by sources of reinforcement, variable neuroadaptive mechanisms, and neurochemical changes that together lead to altered homeostasis of the brain reward system. These behavioral abnormalities reflect neuroadaptive changes in signal transduction function and cellular gene expression produced by repeated drug exposure. To provide a better understanding of addiction and the mechanism of the rewarding effect, it is important to identify related genes. In the present study, we performed gene expression profiling using microarray analysis in a reward effect animal model. We also investigated gene expression in four important regions of the brain, the nucleus accumbens, striatum, hippocampus, and cingulated cortex, and analyzed the data by two clustering methods. Genes related to signaling pathways including G-protein-coupled receptor-related pathways predominated among the identified genes. The genes identified in our study may contribute to the development of a gene modeling network for methamphetamine addiction.

• 한국고분자학회:학술대회논문집
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• 한국고분자학회 2006년도 IUPAC International Symposium on Advanced Polymers for Emerging Technologies
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• pp.367-367
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• 2006

With poly (ethylene-co-methacrylic acid) ionomer (i.e. Surlyn) as a compatibilizer, PP/organoclay (Cloisite(R)20A) nanocomposites were prepared via melt compounding in a co-rotating twin-screw extruder. For comparison, the widely used PP-g-MA was also used as a reference. The content of organoclay was fixed at 5phr based on the total weight of polymer resins. The structures of nanocomposites were characterized by XRD, rheometry in small amplitude oscillatory shear, SEM, and TEM, respectively. It was found that PP/Surlyn/OMMT nanocomposites displayed higher intercalation degree and better dispersion effect than the corresponding PP/PP-g-MA/OMMT counterpart. Additionally, their mechanical properties and wettability were measured.

• 대한수의학회지
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• 제43권3호
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• pp.323-331
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• 2003

We performed this study to determine the late biological effect of gamma radiation and effect of Bu-Zhong-Yi-Qi-Tang (BZYQT), a prescription of traditional Oriental medicine, on radiation-induced late biological effect (survival, hematological change, carcinogenesis) of mice irradiated with 3 Gy of gamma-radiation. There were little difference in body weights between normal and irradiated mice. Survival rate were decreased in irradiated mice and the survival rate and mean survival time of the groups treated with BZYQT were far better than the irradiation control group. A significant elevation of total leukocyte or lymphocyte counts was seen at week 4 and 12 of the group treated with BZYQT. Stimulated recovery by the extract from BZYQT was also observed in thrombocyte. Main gross findings of irradiated mice were appeared as enlargement of spleen, thymus and liver, tumorous nodules of lung and cyst or mass of ovary. Microscopically, there were various findings including hematopoietic and lymphoid tumor, lung cancer, ovarian cancer and cancer of other lesions. BZYQT reduced the incidence of tumor development. Further studies are needed to characterize better the protective nature of ingredients and active compounds.

• 한국축산식품학회:학술대회논문집
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• 한국축산식품학회 2000년도 정기총회 및 제25차 춘계학술발표회
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• pp.101-101
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• 2000

• 한국동물학회:학술대회논문집
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• 한국동물학회 2003년도 한국생물과학협회 학술발표대회
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• pp.150.3-150
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• 2003

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• 한국생명과학회:학술대회논문집
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• 한국생명과학회 2001년도 제31회 학술심포지움 및 춘계학술대회
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• pp.30-30
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• 2001