• Journal of Microbiology and Biotechnology
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• 제15권3호
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• pp.461-465
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• 2005

Characteristics of biological $CO_2$ fixation by Chlorella sp. HA-1 were investigated in a semi-continuous and series reactor system using an internally illuminated photobioreactor to overcome shortcomings of physicochemical technologies such as adsorption and membrane separation. High $CO_2$ fixation rate was achieved in the semi-continuous reactor system, in which the dilution ratios of the culture medium were controlled. The average $CO_2$ fixation rate was maintained almost constantly when the dilution ratio increased by 0.1 increment from the initial value of 0.5. The total removal efficiency of $CO_2$ was enhanced by employing a series reactor system. The average $CO_2$ fixation rate increased until 4.013 g $CO_2\;day^{-1}$ in a series operation of four reactors, compared to 0.986 g $CO_2\;day^{-1}$ in a batch operation mode. The total $CO_2$ fixation rate was proportional to the number of reactors used in the series reactor system. In the series reactor system of semi-continuous operation, a large amount of $CO_2$ was removed continuously for 30 days. These results showed that the present reactor systems are efficient and economically feasible for a biological $CO_2$ fixation.

• Biotechnology and Bioprocess Engineering:BBE
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• 제8권6호
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• pp.354-359
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• 2003

$CO_2$fixation by microalgae has emerged as a promising option for $CO_2$mitigation. In-tensive research work has been carried out to develop a feasible system for removing $CO_2$from industrial exhaust gases. However, there are still several challenging points to overcome in order to make the process more practical. In this paper, recent research activities on three key technologies of biological $CO_2$fixation, an identification of a suitable algal strain, development of high efficient photobioreactor and utilization of algal cells produced, are described. Finally the barriers, progress, and prospects of commercially developing a biological $CO_2$fixation process are summarized.

• KSBB Journal
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• 제18권4호
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• pp.340-345
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• 2003

Feasibility of large-scale photobioreactors for biological CO$_2$ fixation was investigated using Chlorella sp. HA-1. Generally, as the volume of photobioreactor increased, the CO$_2$ fixation rate decreased because of a lower illumination efficiency in large-scale than in small-scale photobioreactors. Though controlling the arrangement and the number of light source, the maximum CO$_2$ fixation rates that could be achieved were 530 and 357 gCO$_2$/㎡day for 40 L and 188 L photobioreactor, respectively, which was higher than the CO$_2$ fixation rate of lab-scale photobioreactor.

• KSBB Journal
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• 제13권1호
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• pp.101-107
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• 1998

Carbon dioxide is estimated to be responsible for 60% of the global warming effect, and this percentage is tending upward. Studies on removal and fixation of $CO_2$ in the flue gas are recognized as one of the important roles of the future biotechnology. Photobiological systems have considerably higher photosynthetic efficiency than conventional biomass system. The experiment for the photosynthetic fixation of $CO_2$ and the biomass production was performed with various initial cell concentration in a tubular photobioreactor and a bubble column $CO_2$ contactor with a gas sparger of $CO_2$ -enriched air(0.03~20%). Synechocystis PCC 6803 could grow at 10~20% $CO_2$ content under pH control. The highest specific growth rate, 0.0258 $h^{-1}$ , was obtained at 5% $CO_2$-air mixture. The maximum cell production rate, 0.2784 g/L.day, was obtained when the initial cell concentration was 0.45 g/L at 5% $CO_2$ -air mixture. The maximum cell concentration was 2.03 g/L in the tubular photobioreactor when the light intensity was $45.5{\mu}$ $E/m^2$ . s. This system showed 0.482 g $CO_2$ /L . day of the $CO_2$ fixation.

• KSBB Journal
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• 제15권6호
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• pp.644-648
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• 2000

Biological fixation of carbon dioxide using microalgae is known as an effective CO$_2$reduction technology. However, many environmental factors influence microalgal productivity. Optimal cultivation factors were determined for the green alga, Euglena gracilis Z, which offers high protein and vitamin E content for animal fodder. In batch culture in a photovioreactor, it was found that theinitial pH, temperature, CO$_2$concentration in air, and light intensity during the optimal cultivating conditions were 3.5, 27$^{\circ}C$, 5-10% and 520 ${\mu}$mol/㎡/s, respectively. When tap water and freshwater were used as cultivating media unsterilized tap water was found to be effective. A kinetic model was considered to determine the relationship between the specific growth rate and the light intensity. The half-velocity coefficient (K(sub)I) in the Monod model under photoautotrophic conditions was 978.9 ${\mu}$mol/㎡/s.

• Journal of Microbiology and Biotechnology
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• 제11권5호
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• pp.772-775
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• 2001

A highly $CO_2$ tolerant microalga, Chlorella KR-1, has been isolated and used to fix $CO_2$ from actual flue gas. Growth of Chlorella KR-1 with the supply of flue gas from a liquified natural gas boiler was comparable to that obtained with 10% $CO_2$. Chlorella KR-1 produced from $CO_2$ fixation using the flue has about 50% crude protein with balanced amino acid profiles. Toxicity was not detected when the microalga was used as a feedstuff for chicks. These results indicate that the KR-1 cells could be a favorable protein source for poultry.

• Applied Biological Chemistry
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• 제11권
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• pp.29-33
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• 1969

1. The oxidizability of $NADPH_2$ by quinones in the presence of $NADPH_2$-diaphorase was tested under aerobic conditions. Also the $^{14}CO_2$-fixation rates were compared when Chlorella suspensions were pretreated with $3{\cdot}10^{-5}M$ cocentration of variou quinones for 10 minutes prior and during the $^{14}CO_2$-fixation period. 2. A close correlation seems to exist between the rate of $NADPH_2$ oxidation by quinones and the $^{14}CO_2$-fixation rate. The effect of quinones on $NADPH_2$ oxidation and $^{14}CO_2$-fixation were in the order of Dichlone>06-K>NQ>BQ. 3. It is postulated that the phytotoxicity of quinones on Chlorella is due to the deprival of $NADPH_2$ consequently inhibiting $^{14}CO_2$-fixation, thus causing death of the cells. 4. The effect of quinones on amino acids biosyn-thesis in Chlorella was one of depressed rates, which was especially noted in the case of dichlone. This would be expected from a consideration of $NADPH_2$ deprival and inhibition of $^{14}CO_2$-fixation. Sucrose synthesis was either not affected or rather stimulated, the reasons of which are not clear at the present time.

• Journal of Marine Bioscience and Biotechnology
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• 제16권1호
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• pp.36-44
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• 2024

This study attempted to improve the growth of the freshwater microalgae, Parachlorella kessleri, through the sequential optimization of culture conditions. This attempt aimed to enhance the microalgae's ability to fixate atmospheric CO₂. Culture temperature and light intensity appropriate for microalgal growth were scanned using a high-throughput photobioreactor system. The supplied air flow rate varied from 0.05 to 0.3 vvm, and its effect on the growth rate of P. kessleri was determined. Next, sodium phosphate buffer was added to the culture medium (BG11) to enhance CO₂ fixation by increasing the availability of CO₂(HCO₃^-) in the culture medium. The results indicated that optimal culture temperature and light intensity were 20℃-25℃ and 300 μE/m²/s, respectively. Growth rates of P. kessleri under various air flow rates highly depended on the increase of the culture's flow rate and pH which determines CO₂ availability. Adding sodium phosphate buffer to BG11 to maintain a constant neutral pH (7.0) improved microalgal growth compared to control conditions (BG11 without sodium phosphate). These results indicate that the CO₂ fixation rate in the air could be enhanced via the sequential optimization of microalgal culture conditions.

• KSBB Journal
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• 제14권6호
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• pp.742-746
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• 1999

The microalgal, Chlorella sp. HA-1, had good $CO_2$ fixation efficiency compared to other algal strains at the same operating condition. In this study, Chorella sp. HA-1 showed similar tolerance both 10% and 20% $CO_2$ concentration. By optimization of the major operation variables such as pH, initial cell concentration, light intensity, the $CO_2$ fixation rate could be raised to a reasonably high value, 372 $gCO_2/m^2{\cdot}day$ in a 3 L internally illuminated photobioreactor. In order to maintain the $CO_2$ fixation rate for a long time, the method of semi-continuous operation was employed, in which dilution ratio was the controlling parameter. Starting with the dilution ratio of 0.5 with the increased increment of 0.1, the constant $CO_2$ fixation rate was obtained.

• Korean Chemical Engineering Research
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• 제44권1호
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• pp.46-51
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• 2006

As one of the $CO_2$ reduction strategies, a biological method was proposed to convert $CO_2$ to useful biomass with antioxidant carotenoids by photosynthetic microorganisms. One of the photoautotrophs, Haematococcus pluvialis is a freshwater green microalga and accumulates the secondary carotenoid astaxanthin during induction of green vegetative cells to red cyst cells. In this study, $CO_2$ fixation and astaxanthin production using H. pluvialis was conducted by photoautotrophic culture in the $CO_2$ supplemented photo-incubator. Maximum growth rate of H. pluvialis was obtained at a 5％ $CO_2$ environment on basic N and P conditions of NIES-C medium. The photoautotrophic induction consisted of 5％ $CO_2$ supply and high light illumination promoted astaxanthin synthesis in H. pluvialis, yielding an astaxanthin productivity of $9.6mg/L{\cdot}day$ and a $CO_2$ conversion rate of $27.8mg/L{\cdot}day$ to astaxanthin. From the results the sequential photoautotrophic culture and induction process using H. pluvialis is expecting an alternative $CO_2$ reduction technology with a function of valuable biosubstance production.