• Title/Summary/Keyword: biological

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Improvement of Biological Control against Bacterial Wilt by the Combination of Biocontrol Agents with Different Mechanisms of Action

  • Kim, Ji-Tae;Kim, Shin-Duk
    • Journal of Applied Biological Chemistry
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    • v.50 no.3
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    • pp.136-143
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    • 2007
  • Despite the increased interests in biological control of soilborne diesease for environmental protection, biological control of bacterial wilt caused by Ralstonia solanacearum have not provided consistent or satisfying results. To enhance the control efficacy and reducing the inconsistency and variability, combinations of specific strains of microorganisms, each having a specific mechanism of control, were applied in this study. More than 30 microorganisms able to reduce the activity of pathogen by specific mechanism of action were identified and tested for their disease suppressive effects. After in vitro compatibility examinations, 21 individual strains and 15 combinations were tested in the greenhouse. Results indicated three-way combinations of different mode of control, TS3-7+A253-16+SKU78 and TS1-5+A100-1+SKU78, enhanced disease suppression by 70%, as compared to 30-50% reduction for their individual treatments. This work suggests that combining multiple traits antagonizing the pathogen improve efficacy of the biocontrol agents against Ralstonia solanacearum.

Bacillus subtilis Spore Surface Display Technology: A Review of Its Development and Applications

  • Zhang, Guoyan;An, Yingfeng;Zabed, Hossain M.;Guo, Qi;Yang, Miaomiao;Yuan, Jiao;Li, Wen;Sun, Wenjin;Qi, Xianghui
    • Journal of Microbiology and Biotechnology
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    • v.29 no.2
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    • pp.179-190
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    • 2019
  • Bacillus subtilis spore surface display (BSSD) technology is considered to be one of the most promising approaches for expressing heterologous proteins with high activity and stability. Currently, this technology is used for various purposes, such as the production of enzymes, oral vaccines, drugs and multimeric proteins, and the control of environmental pollution. This paper presents an overview of the latest developments in BSSD technology and its application in protein engineering. Finally, the major limitations of this technology and future directions for its research are discussed.

First complete mitogenome sequence of Korean Gloydius ussuriensis (Viperidae: Crotalinae)

  • Hye Sook Jeon;Min Seock Do;Jung A Kim;Yoonjee Hong;Chae Eun Lim;Jae-Hwa Suh;Junghwa An
    • Journal of Species Research
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    • v.13 no.2
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    • pp.127-130
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    • 2024
  • The first complete mitogenome sequence of the Red-tongue Pit Viper (Gloydius ussuriensis) from Korea was characterized using next-generation sequencing. The mitogenome is a circular molecule (17,209 bp) with a typical vertebrate mitogenome arrangement, which consists of 2 ribosomal RNA genes (rRNA), 22 transfer RNA genes (tRNA), two non-coding regions (D-loop), and 13 protein-coding genes (PCGs). The base composition of the mitogenome is 32.7% of A, 27.5% of C, 13.9% of G, and 25.9% of T, with a slight AT bias(58.6%). This phylogenetic analysis infers that G. ussuriensis is in the same group as the Chinese G. ussuriensis (Accession No. KP262412) and is closely related to G. blomhoffi and other species of the genus Gloydius. In our study, the complete mitogenome sequence of Korean G. ussuriensis was characterized and we provided basic genetic information on this species.