• 한국임상수의학회지
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• 제32권6호
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• pp.499-503
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• 2015

본 연구의 목적은 건강한 한우 송아지의 동맥과 정맥혈액의 혈액가스, 전해질, 생화학 및 혈액학적 측정치를 조사하는 것이다. 건강한 3주령 이내의 62두 송아지를 본 연구에 공시하였으며 동맥혈액은 귀(이)동맥에서, 정맥혈액은 경정맥에서 채취하였다. 채혈한 혈액은 현장에서 즉시 휴대용 혈액가스분석기(EPOC$^{(R)}$ blood analysis, Woodly Equipment Company Ltd, Lancashire, UK)를 이용하여 pH, $pO_2$, $pCO_2$, $cHCO_3{^-}$, BE, $cSO_2$, $Na^+$, $Ca^{2+}$, $Cl^-$, anion gap potassium (AgapK), Hct, cHgb, glucose, lactate 및 creatinine을 측정하였다. 한우 송아지의 동맥과 정맥의 혈액가스, 전해질, 생화학 및 혈액학적 측정치는 기존에 발표된 정상 송아지의 측정치와 유사한 범위 내에 있었으며 glucose와 lactate의 평균 농도는 성우의 농도보다 높았다. 3주령 이내 송아지의 각 주령별 측정치 사이에는 통계적인 유의차가 나타나지 않았다(P > 0.05). 포도당 농도는 동맥과 정맥 혈액 사이에 가장 높은 상관관계를 보여주었으며(r = 0.927), creatinine (r = 0.925), lactate (r = 0.815), $Ca^{2+}$ (r = 0.806), Hct (r = 0.799), $Na^+$ (r = 0.790), cHgb (r = 0.786), base excess (r = 0.749), pH (r = 0.710), $HCO_3{^-}$ (r = 0.710), 및 $cTCO_2$ (0.663)도 높은 상관관계를 나타내었다. 휴대용 혈액가스분석기로 목장현장에서 혈액을 검사하는 것은 빠른 측정 결과를 얻을 수 있으며 소임상에서 유용하게 활용될 수 있을 것으로 생각된다.

• 대한약침학회지
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• 제9권1호
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• pp.5-20
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• 2006

Objectives : This study was carried out to identified the effects of distilled cultivated wild ginseng pharmacopuncture to the obesity. Methods : Cultivated wild ginseng pharmacopuncture was administered on the points of chung-wan(CV12), $Ch'{\breve{o}}nch'u$(ST25), and Chok-samni(ST36) on lowering lipid and oxidative capacity in biochemical and molecular biological aspects were investigated in obese rats fed with high fat diet. Results : 1. The contents of plasma ${\beta}-lipoprotein$ showed a tendency to decrease in the pharmacopuncture groups compared to the control group. In the pharmacopuncture groups, the values of ST25 and ST36 pharmacopuncture groups showed lower value. 2. The contents of plasma free fatty acids showed a tendency to decrease in pharmacopuncture groups compared to the control group. However, in the pharmacopuncture groups, the values were not significantly different. 3. Plasma triglyceride and glucose showed lower value in the ST25 pharmacopuncture groups compared with the other groups. 4. The activity of AST showed a tendency to decrease in the pharmacopuncture groups. However, the activity of ALT was not significantly different in all the treatment groups. 5. Plasma total cholesterol and LDL-cholesterol showed lower value in the ST25 and ST36 pharmacopuncture groups and HDL-cholesterol showed higher value in the CV12 pharmacopuncture groups than that of the other treatment groups. 6. Liver total cholesterol values didn't show significant difference in all the treatment groups, and triglyceride showed lower value in the pharmacopuncture groups. 7. The contents of plasma TEARS showed lower value in the ST25 pharmacopuncture group and contents of liver TBARS showed a tendency to decrease in the pharmacopuncture groups. However these values didn't show significant difference in the pharmaco puncture groups. 8. Liver super oxide dismutase activity showed higher value in the ST25 and ST36 pharmacopuncture groups, and the value of liver glutathione peroxidase and catalase activity showed a tendency to increase in the pharmacopuncture groups. However, these values showed no significant difference in the pharmacopuncture groups. 9. Expression of apo-B and E mRNA in liver cells was lower in the ST25 pharmacopuncture group than that of the other treatment groups. However, expression of $TNF-{\alpha}$ and leptin mRNA in adipose cell showed no difference among all the treatment groups. 10. ST25 pharmacopuncture group showed a good histological character of liver. It showed similar to that of normal group. However other treatment groups and control group showed slight vasodilation and slight fat accumulation. Conclusion : These results indicate that distilled cultivated wild ginseng pharmacopuncture suppressed adipose tissue mass and lipid peroxidation, and increased antioxidant capacity.

• 대한수의학회지
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• 제28권1호
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• pp.165-168
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• 1988

Some hematological and biochemical indices were assayed in Korean native cattle naturally harbouring Fasciola hepatica infection and compared with uninfected controls. Affected animals revealed reduction in total erythrocyte count and hemoglobin content, and increase in total leukocyte count. Infected cattle had significantly lower levels of serum total protein, serum albumin and magnesium. Significantly higher values were obtained for the serum enzymes aspartate aminotransferase and alanine aminotransferase.

• 한국임상수의학회지
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• 제8권1호
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• pp.71-80
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• 1991

Clinical signs, serum chemical values and histological findings of hepatic tissue after partial and complete obstruction of common bile duct in Korean goats were investigated. Abnormal clinical signs were not observed in partial obstruction of common bile duct, but in complete obstruction clinical signs such as jaundice, urine color change, were observed. Serum total bilirubin, total cholesterol, aspartate aminotransferase, sorbitol dehydrogenase, gamma glutamyltranspeptidase, and total protein values increased on the 1-4th day and then gradually decreased to normal level in partial obstruction. However, they tend to increase persistently by the 24th day in complete obstruction of common bileduct. Histologic features of hepatic tissue in partial obstruction were not changed as compared with normal hepatic tissue. On the other hand, in complete obstruction of common bite duct there were moderate bile duct proliferation in a portal area, rupture of bile canaliculi, phagocytosis of bile pigment by Kupffer cells, periportal fibrosis, intrahepatic bile stasis and hepatic cell necrosis.

• Environmental Analysis Health and Toxicology
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• 제11권3_4호
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• pp.11-16
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• 1996

Paraquat is a useful nonselective herbicide widely used throught the world. However accidental or intentional ingestion of the herbicide cause fatal pulmonary injuring. But there is not suitable antidote of paraquat intoxication and therapeutic agents now be used are not effective. So, in this study we intended to evaluate the inhibitory effects of acetylmannan from Dioscorea batalas on paraquat toxicity. 100mg/kg acetylmannan from wild or cultured Dioscorea bataras was administered orally to male SD rats for 3 days and the administration time interval was 24hours. After one hour of final administration, 50mg/kg paraquat was administered intraperitonially. After 24 hours, the biochemical parameters of blood and tissues were examined. In paraquat treated groups, sGPT, BUN, creatinine, ALP levels were increased by 2 to 4 times of normal values. However in acetylmannan from wild Dioscorea batatas treated groups, sGPT, BUN, creatinine, ALP levels in blood and lung tissue were significantly decreased to normal levels. In acetylmannan from cultured Dioscorea batatas treated groups, BUN, creatinine were significantly decreased to normal values, but not in sGPT, ALP levels. Therefore, we concluded that acetylmannan from wild Dioscorea batatas can be used as an. antidote of paraquat toxicity.

• 한국임상수의학회지
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• 제14권2호
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• pp.268-272
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• 1997

This study was performed to estimate the clinical signs and biochemical para meters of indicator on acute hepatic injury induced by the administration of CCl$_{4}$ in rats. Minor behavioral changer brittleness of skin hair and decreased volume of water and food intake were observed in rats after 2 hours of $CCl_{4}$ administration compared to control group. Concentration of serum albumin has shown lower than that of control group. However concentration of total bilirubin has shown higher than that of control group. As times go onto serum LDH activity was significantly increased compared to control Broup. Serum CPK activity hasn't shown change compared to control group. Passive hemagglutination that of afetoprotein was shown negative reaction in all the treatment groups and control group.

• 한국임상수의학회지
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• 제15권1호
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• pp.75-78
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• 1998

This study was performed to estimate the clinical signs and changes of biochemical parameters in rats with hepatic injury induced by the administration of 2, 2'-azobis(2- amidinopropane)dihydrochloride (AAPH) . Minor behavioral change, brittleness of skin hair and decreased volume of water and feed intake were observed in ra% 2 hours after AAPH administration compared to control group. Concentration of serum albumin showed lower than that of control group. However, concentration of total bilirubin has shown higher than that of control group. As time goes on, . serum LDH activity increased significantly compared to control group, but senun CPK activity did not change compared to control group. Passive hemanglutination of $\alpha $-fetoprotein was negative in all the treaDent groups and control group.

• 대한약침학회지
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• 제4권3호
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• pp.85-92
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• 2001

Objective : This study was purposed to investigate the acute. subacute toxicity of Herbal acupuncture with Juglandis Semen(JsD) in mice and rats. Methods & Results: Balb/c mice were injected intraperitoneally with JsD for $LD_{50}$ and acute toxicity test Sprague-Dawley rats were injected intraperitoneally with JsD for subacute toxicity test. Results: The results obtained were summarized as follows; 1. LD50 was uncountable as could not find the expired of treat group. 2. The clinical signs and body weight changes of mice treated with 0.2cc, 0.4cc JsD were not affected during the acute toxicity test. 3. In acute toxicity test of serum biochemical values of mice, total protein was increased in treat-l group, compared with normal group, and total cholesterol was increased in treat-2 group, compared with normal group.(P<0.05) 4. In subacute toxicity test, main toxic syndrome was not found. 5. The body weight was decreased in treat-2 group, compared with normal group and relative liver weight was decreased in treat-1, 2 group, compared with normal group in subacute toxicity test.(P<0.05) 6. In subacute toxicity test, WBC, MCH, MCHC were decreased in treat-2 group and RBC was increased in treat-2 group, compared with normal group in complete blood count test.(P<0.05) 7. In subacute toxicity test, treat groups were not changed serum biochemical values of rats, compared with normal group.(P<0.05) Conclusions: According to the results, Herbal-acupuncture with Juglandis Semen caused no toxicity.

• 한국동물위생학회지
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• 제33권4호
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• pp.411-415
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• 2010

The present study was devised to determine the effects of body weight, organ weight, hematological values and biochemical parameters by vitamin E and selenium (Selevit) in the ovariectomized (OVX) rats. The animals were divided into 4 groups. Intact group (n=10) received no treatment and operation. Sham group (n=10) received only sham operation and no treatment. OVX group received operation and no treatment. OVX+Selevit received operation and Selevit. The body weights of the all group increased, and that of OVX+Selevit group was the lowest rates. There were significant differences (P<0.01, P<0.001) of body weights between OVX+Selevit group and all other groups. Also, organ weights such as heart, liver, spleen and kidney were measured. The heart and liver weight were significantly lower (P<0.01, P<0.001) in the OVX+Selevit group than in the Intact and Sham group. Also, the kidney weight was significantly lower (P<0.05, P<0.01) in the OVX+Selevit group than in the all other group. On the other hand, there was no significantly difference in the organ weight of spleen between the OVX+Selevit group and the other groups. The number of WBC was significantly higher (P<0.05) in the OVX+Selevit group than in the all other groups. The hematological values of RBC, MCV, MCH and MCHC were no significant differences in any groups. The biochemical parameters of serum total protein and alkaline phosphatase increased significantly in the OVX+Selevit group as compared to that in the OVX group. But, there were no significant differences in AST and ALT in any groups. We conclude that vitamin E and selenium were significantly decreased the body weights in the ovariectomized rats. Our findings suggest that vitamin E and selenium may influence the process of lipid packaging and absorption in the ovariectomized rats.

• 생약학회지
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• 제29권4호
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• pp.283-292
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• 1998

The hemopoietic effects of deer blood (whole blood, blood cell, plasma, respectively) were examined using in vivo rat model. Experimental animals (Sprague-Dawley rat, male, 200 g) were divided into negative control group (injection of saline), positive control group (injection of Sipjeondaebotang) experimental groups (injection of whole blood, blood cell, plasma) and healthy control group. Cyclophosphamide(150mg/kg) was injected into experimental groups, negative and positive control group to induce bone marrow supression. After 8 days, freez dried deer blood (whole blood, blood cell, plasma respectively) and Sipjeondaebotang of 200 mg/kg in dose was administered orally into experimental groups and positive control group, once a day for 3 days (A group) and once a day for 12 days (B group) respectively. And then body weight and organ weight, biochemical profile (ALB, GOT, GPT, PRO, CRE), hematological values (WBC, RBC, HGB, HCT, MCV, MCH, MCHC, PLT) and CBC differentiation (Neutro, Lymph, Mono, Reticulo) were carried out. Finally, platelets were specially increased in the plasma treated A group and reticulocytes were specially increased in the plasma treated B group.