• Title/Summary/Keyword: biochemical and cultural properties

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Biochemical properties and cultural characteristics of Escherichia coli isolated from chickens (닭에서 분리한 Escherichia coli의 생물화학적 및 배양 특성)

  • Woo, Yong-ku;Kim, Ki-seuk;Kim, Bong-hwan
    • Korean Journal of Veterinary Research
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    • v.30 no.4
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    • pp.421-425
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    • 1990
  • The present study was conducted to investigate the biochemical and cultural characteristics of Escherichia coli isolates from clinically affected chickens during the period from May 1988 to June 1989. A total of 82 E coli cultures were isolated from lesions of 75 chickens with colisepticemia. Biochemical properties of E coli isolates tested were in accordance with the general classification standard; all the isolates showed positive reaction in Catalase, Indol, and Methyl-Red tests, but negative reaction in Oxidase, Urease, $V{\ddot{o}ges$-Proskauer, Citrate utility, $H_2S$, Phenylalanine diaminase, and malonate tests. And the carbohydrate fermentation rates of them were shown to be variable. of the 82 isolates, 48(58.5%) cultures produced colicin to inhibit the indicator strain of E coli.

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Biochemical Properties and Antimicrobial Drug Susceptibility of Pasteurella haemolytica Isolated from Pneumonic Calves (호흡기질병 감염 송아지에서 분리한 Pasteurella haemolytica의 생화학적 특성 및 약제 감수성)

  • 조광현;박노찬;권헌일;김이준;박덕상
    • Korean Journal of Veterinary Service
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    • v.14 no.2
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    • pp.121-126
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    • 1991
  • The present study was conducted to investigate biochemical properties and antimicrobial drug susceptibilities of 36 strains of Pasteurella haemolytica(P haemolytica) isolated from pneumonic calves in Kyongbuk province during the period from January 1990 to December 1990. P haemolytica was isolated from 36 of 111(32.4%) pneumonic calves of 1 to 6 months of age. The majority of biochemical and cultural properties of P haemolytica isolated from calves were identical to those of the reference strains employed. All isolated were susceptible to baytril, gentamicin, and kanamycin, some of them were resistant to tetracycline, amikacin, streptomycin, and ampicillin.

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Biochemical properties and antimicrobial drug susceptibility of Erysipelothrix rhusiopathiae isolated from the cases of acute septicemic swine erysipelas (급성 폐혈증형 돼지단독예에서 분리한 Erysipelothrix rhusiopathiae의 생화학적 특성 및 약제감수성)

  • Baek, Young-sook;Cho, Gil-jae;Kim, Bong-hwan
    • Korean Journal of Veterinary Research
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    • v.30 no.4
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    • pp.413-420
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    • 1990
  • The present study was conducted to investigate biochemical properties and antimicrobial drug susceptibilities of 47 strains of E rhusiopathiae isolated from the cases of acute septicemic swine erysipelas in Youngnam and Kyunggi provinces during the period from June 1988 to December 1989. The isolants were identified as E rhusiopathiae on the basis of cellular and colonial morphology, and characteristic reactions in some biochemical tests. All the organisms produced hydrogen sulfide in triple sugar iron agar and showed the characteristic "pipe cleaner" type of growth in gelatin stab cultures. The majority of biochemical and cultural properties of E rhusiopathiae isolated from pigs affected with acute erysipelas were identical to those of the reference strains employed. All the isolates were highly susceptible to penicillin G, ampicillin, erythromycin (MIC:0.025~0.39IU or ${\mu}g/ml$), and moderately susceptible to oleandomycin, oxytetracycline, chloramphenicol(MIC:$0.78{\sim}25{\mu}g/ml$). Kanamycin and sulfadimethoxine showed no activity against the isolates(MIC:>$400{\mu}g/ml$). The MICs of dihydrostreptomycin presented two distribution peaks; of 47 strains, 5(10.6%) were resistant to dihydrostreptomycin (MIC:$400{\mu}g/ml$), while the majority of them were susceptible to the drug.

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Biochemical properties and antimicrobial susceptibility of Pasteurella multocida isolated from pneumonic lungs in slaughtered animals (도축장 출하축의 폐병변에서 분리한 Pasteurella multocida의 생화학적 특성 및 약제감수성)

  • 김성국;이양수;정종식;김수웅
    • Korean Journal of Veterinary Service
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    • v.22 no.1
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    • pp.79-84
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    • 1999
  • This study was carried out to investigate isolation of Pasteurella multocida from pneumonic lungs of slaughtered animals in Kyungsan abattoir to examine the antimicrobial susceptibility and biochemical properties. The results were summarized as follows ; P multocida was isolated from 50(29.2% ) of the 171 pneumonic lungs collected individually from cattle(3/40), goats(0131) and pigs(47/100), All of the isolated P multocida showed biocemical and cultural properties similiar to reference strains. All isolates were very susceptible to AK, AM, ENR, CF, GM,07, whereas resistant to SDM.

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Biochemical properties and antimicrobial drug susceptibility of Pasteurella haemolytica isolated from pneumonic calves and goats (호흡기 증상을 나타낸 송아지 및 산양에서 분리한 pasteurella haemolytica의 생화학적 특성 및 약제감수성)

  • Cho, Kwang-hyun;Kim, Bong Hwan
    • Korean Journal of Veterinary Research
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    • v.31 no.4
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    • pp.449-456
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    • 1991
  • The present study was conducted to investigate biochemical properties and antimicrobial drug susceptibilities of 57 strains of Pasteurella haemolytica (P haemolytica) isolated from pneumonic calves and goats in Youngnam province during the period from September 1989 to February 1991. P haemolytica was isolated from 95 of 142(31.7%) pneumonic calves of 1 to 6 months of age and from 9 of 24(37.5%) pneumonic goats of 2 to 10 months of age. Seasonal isolation frequency of P haemolytica in calves and goats varied from 16.6 to 35.7%, and it was higher in winter. The majority of biochemical and cultural properties of P haemolytica isolated from calves and goats were identical to those of the reference strains employed. All isolates were susceptible to ceftiofur, erythromycin, kanamycin, gentamicin, and chloramphenicol (MIC: <$25{\mu}g/ml$), some of them were resistant to amikacin, sulfadimethoxin, and streptomycin (MIC: >200IU or ${\mu}g/ml$).

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Incidence of Bordetella bronchiseptica Infection in Youngnam Swine Herds and the Biochemical Properties of the Organisms Recovered from Pigs with Atrophic Rhinitis (영남지방 돼지의 Bordetella bronchiseptica 감염상황 및 분리균의 생화학적 특성)

  • Jang, Hee-kyung;Kim, Bong Hwan
    • Korean Journal of Veterinary Research
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    • v.28 no.1
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    • pp.75-81
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    • 1988
  • The present study was conducted to investigate the Bordetella bronchiseptica infection in Youngnam swine herds during the period from August 1986 to July 1987 and some properties of the organisms isolated from these Korean swine. B. bronchiseptica was recovered from 25 of 70 (35.7%) glowing pigs of 4 to 10 weeks of age and from 12 of 13 (92.3%) herds. From 115 slaughter pigs, 58(50.4%) pigs were culture positive and the pigs from 13 of 14 (92.9%) herds were found to be infected with B. bronchiseptica. The majority of biochemical and cultural properties of B. bronchiseptica isolated from Korean swine were identical to those of the standard strain employed and some 97.6% of the isolates showed the characters of phase I organism on primary isolation.

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Isolation and antimicrobial susceptibility test of Pasteurella multocida from respiratory disorder piglets (호흡기증세 자돈으로부터 파스튜렐라 속균 분리 및 약제감수성시험)

  • Lee Woo-Won;Woo Byung-Gil;Kim Hong-Tae;Lee Gang-Rok;Lee Dong-Soo
    • Korean Journal of Veterinary Service
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    • v.27 no.3
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    • pp.257-263
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    • 2004
  • This study were carried out to investigate isolation of Pasteurella multocida from respiratory disorder piglets, to examine the biochemical properties and antimicrobial susceptibility. The results were summarized as follows; P multocida was isolated from 31($10.3\%$) of the 302 respiratory disorder or growing piglets of $4{\sim}10$ week olds. The majority of biochemical and cultural properties of the P multocida isolates were identical to those of the standard strains. The isolates were highly susceptible to norfloxacin($100.0\%$), enrofloxacin($96.8\%$) and ampicillin($87.1\%$), but resistant to streptomycin ($77.4\%$), penicillin($61.2\%$) and neomycin($54.8\%$).

Incidence of Pasteurella multocida infection in Youngnam swine herds and the biochemical properties of the organisms recovered from pigs with atrophic rhinitis and pneumonic lungs (영남지방 돼지의 Pasteurella multocida 감염상태 및 분리균의 생화학적 특성)

  • Cho, Gil-jae;Kim, Bong-hwan
    • Korean Journal of Veterinary Research
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    • v.29 no.4
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    • pp.479-485
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    • 1989
  • The present study was conducted to investigate the incidence of Pasteurella multocida infection in Youngnam swine herds during the period from March 1988 to February 1989 and some properties of the isolated organisms. P multocida was isolated from 22(43.1%) of 51 growing pigs of 4 to 12 weeks of age and from 8(80.0%) of 10 herds. From nasal turbinates of 102 slaughtered pigs, 47(46.1%) pigs were culture positive and pigs from 8(88.9%) of 9 herds were found to be infected with P multocida. From lungs of 101 slaughtered pigs, 42(41.6%) pigs were culture positive and the pigs from 11(91.7%) of 12 herds were found to be infected with P multocida. The majority of biochemical and cultural properties of the P multocida isolates were identical to those of the standard strains. The isolation frequencies of P multocida in relation to pig snout lesion grades of 0 to 5 were 28.6%, 41.6%, 48.0%, 50.0%, 85.7%, and 100%, respectively.

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Antibiotic Resistance and Biochemical properties of Pathogenic Echerichia coli Isolated from Piglets with Diarrhea in Kyongbuk Western Area (경북서부지역 설사자돈에서 분리한 Haemolyic E. coli의 생화학적 특성 및 약제 내성)

  • 조종숙;이정아;오강희;박영구
    • Korean Journal of Veterinary Service
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    • v.16 no.2
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    • pp.150-156
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    • 1993
  • The present study was conducted to investigate the biochemical characteristics and anti-biotic resistance of pathogenic Escherichia coli (E. coli) isolated from piglets with diarrhea in Kyongbuk Western Area during the period from February to November 1992. 55 E. coli strains were isolated from 97 piglets with diarrhea and the biochemical and cultural reaction were compared with the classification criteria of Edwards and Ewing. The majority of E. coli were susceptible to amikacln, enrofloxacin and gentamicin. 51 (92.7%) out of 55 drug resistance stains carried R factor (R+) which were transferable to the recipients by conjugation.

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Prevalence and factors associated with Blackleg outbreak in north western Gyeongbuk province (경북 문경지방에서 발생한 기종저의 역학적 특성)

  • 장영술;김규태;김영환;김순태;도재철;김대원;김봉환
    • Korean Journal of Veterinary Service
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    • v.25 no.1
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    • pp.75-86
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    • 2002
  • Outbreaks from September 1999 to July 2000 of blackleg in 4 milking and fatting cattle farms in the vicinity of Munkyong(northwestern part of Gyeongbuk province) were investigated biochemical characteristics of isolates and were surveyed epidemiological works during the period of 2 months from August to September 2000. Clostridium chauvoei, the etiological agent of blackleg is a serious pathogen of cattle and sheep causing fever, depression and lameness with high mortality in growing phase of 6 to 18 months old mainly beef cattle. Biochemical and cultural properties of 4 isolated Cl chauvoei were identical to those of reported results and isolates were susceptible to ampicillin, bacitracin, chlorarnphenicol, cephalothin, ciprofloxacin, erythromycin, norfloxacin, penicillin, and tetracycline, while they were resistant to amikacin, colistin, gentamicin, kanamycin, neomycin, streptomycin and trimethoprim-sulfamethoxazole. In the results of epidemiological survey, outbreak farms that concentrated region of milking or fatting cattle in each sites were so close contact within a radius of about 1km that they are separated only by wire fence or shrub harrier and flies, rodents, wild cats and birds could have the chance to move around between those farms. The origin of the outbreak blackleg were uncertain, but the transmission between farms were mainly through the movement of farm personnel, vehicles which were struck the feces or manures from farm and other mechanical factors such as flies, rodents and birds furthermore occurred 4 farms were not vaccinated. In conclusion, a periodic disinfection, vaccination and quarantine prevention are ideal methods of damage reduction by blackleg.