• Environmental Analysis Health and Toxicology
• /
• v.29
• /
• pp.18.1-18.9
• /
• 2014

Objectives The purpose of this study was to determine a separation method for each arsenic metabolite in urine by using a high performance liquid chromatography (HPLC)-inductively coupled plasma-mass spectrometer (ICP-MS). Methods Separation of the arsenic metabolites was conducted in urine by using a polymeric anion-exchange (Hamilton PRP X-100, $4.6mm{\times}150mm$, $5{\mu}m$) column on Agilent Technologies 1260 Infinity LC system coupled to Agilent Technologies 7700 series ICP/MS equipment using argon as the plasma gas. Results All five important arsenic metabolites in urine were separated within 16 minutes in the order of arsenobetaine, arsenite, dimethylarsinate, monomethylarsonate and arsenate with detection limits ranging from 0.15 to $0.27{\mu}g/L$ ($40{\mu}L$ injection). We used G-EQUAS No. 52, the German external quality assessment scheme and standard reference material 2669, National Institute of Standard and Technology, to validate our analyses. Conclusions The method for separation of arsenic metabolites in urine was established by using HPLC-ICP-MS. This method contributes to the evaluation of arsenic exposure, health effect assessment and other bio-monitoring studies for arsenic exposure in South Korea.

• Journal of Biosystems Engineering
• /
• v.35 no.4
• /
• pp.244-249
• /
• 2010

This study was conducted to define the optimal Lycium chinense miller washing method for developing the Lycium chinense cleaner and we analyzed the Lycium chinense miller washing characteristics for removing pesticides and microorganism according to washing methods; habitual washing method, air bubble washing method and nozzle spray washing method. The results were summarized as follows; 1. In case of measuring physical properties according to the varieties, maximum yield strength of Hokwang was 2.562 kgf, minimum yield strength of Hokwang was 0.269 kgf and average yield strength was about 1 kgf. 2. In case of measuring change of bacteria according to washing methods, the number of bacteria of non-washing method was more than the number of bacteria of habitual washing method or mechanical washing method and the number of nozzle spray washing method was least. 3. Ahjoksiseuteurobin of 0.218 ppm was detected in the untreated sample, 0.051 ppm was detected in the habitual washing method, 0.047 ppm was detected in the air bubble washing method and 0.034 ppm was detected in nozzle spray washing method. Every amount detected were less than 2 ppm that is reference value and the detected amount was least in case of nozzle spay cleaning method. Cypermethrin of 0.772 ppm was detected in the non-cleaned sample, 0.089ppm was detected in habitual washing method, 0.26 ppm was detected in the air bubble washing method and 0.292 ppm was detected in the nozzle spray washing method. Every detected amount of Cypermethrin were less than 5 ppm that is reference value and the detected amount was least in case of habitual washing method.

• Proceedings of the KSAR Conference
• /
• 2003.06a
• /
• pp.43-43
• /
• 2003

This study was conducted to produce transgenic pig harboring human tissue plasminogene activator (tPA) gene. Two different tPA genes containing bovine $\beta$-casein promoter and mouse uroplakin promoter were prepared for microinjection and confirmed the expression level of tPA protein from the CHO (Chinese hamster ovary) cell lines by gene transfection. Concentration of tPA expression from the six cell lines (all of CHO cells) were average 212.4 ng/ml. Reconstructed DNA to used the CHO cell were microinjected into the pronuclei of in vivo embryos The total of 2,307 zygotes were collected from 95 donors and 1,851 embryos were in 1-cell stage which were visualized the pronuclei for DNA microinjection. The concentration of linear DNA was 2.0 ng per microliter and injected into zygotes with two pronuclei on an inverted Nikon microscope equipped with narishige micromanipulator and modulation contrast optics. The 541 embryos injected with bovine $\beta$-casein promoter-tPA were transferred to 22 recipients. The 1,154 embryos injected with mouse uroplakin promoter-tPA were transferred to 51 recipients. Sixty nine offspring from 9 delivered sows were produced. We analysed the transgenes with PCR methods from 69 offsprings, but could not detect the PCR product from piglet tails DNA.

• Korean Journal of Environmental Agriculture
• /
• v.25 no.4
• /
• pp.382-385
• /
• 2006

To know the effect of water treatment sludge (WTS) as a rice seedling culture soil, rice seeds are sown, germinated and grown on the culture soils mixed with different ratios of WTS to sand (100:0, 50:50, 30:70, respectively), and their growth effects was compared to that of control, commercial culture soil. When WTS is used as a culture soil for rice, the length and weight of the top part of rice seedling somewhat decreased than those of control treatment while their root length and weight in all WTS treatments significantly increased. Although phosphorus and potassium uptake of rice seedling in WTS treatments was slightly decreased, WTS treatment showed to develop root growth which is necessary in the seedling stage of all plane. Such a stimulatory effect on root growth of rice seedling was significant in the mixture ratio of 50% WTS to 50% sand.

• Journal of the Korean Society of Environmental Restoration Technology
• /
• v.19 no.1
• /
• pp.85-100
• /
• 2016

Local Agenda 21 organizations have been founded at the most divisions of local government in Chungcheongbuk-Do, but there has been no methods to monitor the total Sustainable Development trends. For the alternative solution of that problem in the Province, this study purposed (1) to develop Indicators fit to this Province (2) to examine the availability of the developed indicators adopting to each division section. Indicators were developed and examined by fields and Quantity / Quality considerations. As a 1st step, committee-composed with field professionals each-selected and developed Quantitative Sustainable Development Indicators(SDIs) optimized to Chungbuk-Province through critical discussions proposing Qualitative Indicators as a suggestion. In 2nd step, developed Indicators were applied and tested upon each divisions. Statistic data were used to calculate indices. 3rd step is adoption stage of Qualitative Indicators which were tested with questionnaire survey analysis. As a results, SDIs for Chungcheongbuk-Do -38 Quantitative SDIs within 4 provisions of 11 items with 8 Qualitative indicators categorization be put off- is developed. With the application test, 5 Quantitative Indicators- were found to be not adaptable because of lack or none of statistic data. Among the 8 Qualitative Indicators, 3 pairs found to be similar results in each pair which needed to be integrated into one. With them, bio-species related and health related Indicators were proposed to be newly added. Nation wide Sustainability comparison between divisions and Ecological Indicator selection are another themes to be considered.

• Journal of Microbiology and Biotechnology
• /
• v.13 no.4
• /
• pp.607-612
• /
• 2003

To elucidate the action mechanism of MCS-C2, a novel analogue of toyocamycin and sangivamycin, its effect on the expression of cell cycle-related proteins in the human myelocytic leukemia cell line HL-60 was examined using Western blotting and a flow cytometric analysis. MCS-C2, a selective inhibitor of cyclin-dependent kinases, was found to inhibit cell growth in a time- and dose-dependent manner, and inhibits cell cycle progression by inducing the arrest at G1 and G2/M phases, in HL-60 cells. The flow cytometric analysis revealed an appreciable arrest of cells in the G2/M phase of the cell cycle after treatment with MCS-C2. The HL-60 cell population increased gradually from 13% at 0 h, to 28% at 12 h in the G2/M phase, after exposure to $2{\;}\mu\textrm{M}$ MCS-C2. Furthermore, Western blot analysis demonstrated that MCS-C2 induced the cell cycle arrest at G1 phase through the inhibition of pRb phosphorylation. Hypophosphorylated pRb accumulated after treatment with $5{\;}\mu\textrm{M}$ MCS-C2 for 12 h, whereas, the level of hyperphosphorylated pRb was reduced. Thus, treatment of the cell with MCS-C2 suppressed the hyperphosphorylated form of pRb with a commensurate increase in the hypophosphorylated form.

• The Korean Journal of Physiology and Pharmacology
• /
• v.16 no.1
• /
• pp.17-24
• /
• 2012

The hypothalamus-pituitary-adrenocortex (HPA) axis is the central mediator of the stress response. The supramammillary (SuM) region is relatively unique among the hypothalamic structures in that it sends a large, direct projection to the hippocampal formation. It has been shown that mild stress could activate the SuM cells that project to the hippocampus. However, the role of these cell populations in modulating the stress response is not known. The present study examined the effect of stress on different populations of SuM cells that project to the hippocampus by injecting the fluorescent retrograde tracer, fluorogold (FG), into the hippocampus and utilizing the immunohistochemistry of choline acetyltransferase (ChAT), corticotrophin releasing factor (CRF), serotonin (5-HT), glutamate decarboxylase (GAD), tyrosine hydroxylase (TH) and NADPH-d reactivity. Immobilization (IMO) stress (2 hr) produced an increase in the expression of ChAT- immunoreactivity, and tended to increase in CRF, 5-HT, GAD, TH-immunoreactivity and nitric oxide (NO)-reactivity in the SuM cells. Fifty-three percent of 5-HT, 31% of ChAT and 56% of CRF cells were double stained with retrograde cells from the hippocampus. By contrast, a few retrogradely labeled cells projecting to the hippocampus were immunoreactive for dopamine, ${\gamma}$-aminobutyric acid (GABA) and NO. These results suggest that the SuM region contains distinct cell populations that differentially respond to stress. In addition, the findings suggest that serotonergic, cholinergic and corticotropin releasing cells projecting to the hippocampus within the SuM nucleus may play an important role in modulating stress-related behaviors.

• Advances in environmental research
• /
• v.4 no.3
• /
• pp.155-172
• /
• 2015

In this study, a complete set of recirculating cooling water system and the required instruments were built in a semi-industrial-scale and a 50 g/h ozone generation plant and a chlorine system were designed for cooling water treatment. Both chlorination and ozonation treatment methods were studied and the results were analyzed during two 45-days periods. The concentrations of ozone and chlorine in recirculating water were constant at 0.1 mg/lit and 0.6 mg/lit, respectively. In ozone treatment, by increasing the concentration cycle to 33%, the total water consumption decreased by 26% while 11.5% higher energy efficiency achieved thanks to a better elimination of bio-films. In case of Carbon Steel, the corrosion rate reached to 0.012 mm/yr and 0.025 mm/yr for the ozonation and chlorination processes, respectively. Furthermore, consumptions of the anti-corrosion and anti-sedimentation materials in the ozone cooling water treatment were reduced about 60% without using any oxidant and non-oxidant biocides. No significant changes in sediment load were seen in ozonation compared to chlorination. The Chemical Oxygen Demand of the blow-down in ozonation method decreased to one-sixth of that in the chlorination method. Moreover, the soluble iron and water turbidity in the ozonation method were reduced by 97.5% and 70%, respectively. Although no anaerobic bacteria were seen in the cooling water at the proper concentration range of ozone and chlorine, the aerobic bacteria in chlorine and ozone treatment methods were 900 and 200 CFU/ml, respectively. The results showed that the payback time for the ozone treatment is about 2.6 years.

• Journal of The Korean Society of Agricultural Engineers
• /
• v.54 no.2
• /
• pp.137-145
• /
• 2012

본 연구에서는 2005년부터 2006년까지 충청남도 공주시 반포면에 위치한 봉곡천 유역의 경사지 밭을 포함하고 있는 산지하천에서 유출량, 총인, 총질소를 측정하였고 측정된 자료는 SWAT 모형을 통하여 장기간의 배출부하량 산정을 위해 모형의 보정 및 검정자료로 사용하였다. SWAT 모형의 보정 및 검정결과는 유출량은 일별자료를 이용하여 보정 및 검정을 실시하였다. 그 결과 결정계수 ($R^2$)가 0.80~0.83의 값을 보였으며 일별 T-N, T-P 부하량에 대한 보정 및 검정결과는 결정계수 ($R^2$)가 0.62~0.86의 값을 보였다. 모형의 보정 및 검정을 통해 결정된 최적매개변수를 적용하여 1997년부터 2006년까지 관측된 강우자료로 장기간의 유출량, T-N, T-P 배출부하량에 대한 SWAT 모형 시뮬레이션을 수행하였다. 또한 이를 바탕으로 하여 산지와 밭에 대한 원단위를 산정하였으며, 그 결과 산지에 대한 T-N의 원단위는 3.29 $kg/km^2/day$이었고 T-P에 대한 원단위는 0.15 $kg/km^2/day$로 나타났다. 또한 밭에서의 T-N에 대한 원단위는 11.15 $kg/km^2/day$이었고 T-P에 대한 원단위는 0.70 $kg/km^2/day$로 나타났으며 강우의 시간 및 공간적 변화에 따른 유출량을 고려한 산지와 밭에서의 영양염류 배출부하량을 산정하는데 SWAT모형을 적용하는 것이 타당성이 있는 것으로 판단되었다.

• Journal of the Korea Academia-Industrial cooperation Society
• /
• v.15 no.6
• /
• pp.3383-3388
• /
• 2014

An experimental study was performed to compare the characteristics of the combustion pressure and exhaust emissions in the case of using pure diesel when the EGR rate was changed in a CRDI 4-cylinder diesel engine with those using biodiesel blended and pure diesel fuel. In this study, the EGR rate variation were conducted at an engine speed of 2000rpm with fuel with a biodiesel blended rate of 20%. The combustion pressure of the biodiesel blended rate 20% and pure diesel fuels decreased with increasing EGR rate. The IMEP of biodiesel was higher than that of ULSD (Ultra low sulfur diesel). The emission results showed that the NOx emission of biodiesel blended fuel with increasing EGR rate was higher than that of ULSD. In addition, the NOx emission of biodiesel blended and diesel fuel decreased with increasing EGR rate. The CO and soot, $CO_2$ emissions increased with increasing EGR rate, and the CO and soot emissions from the biodiesel blended fuel were lower than that of ULSD but the $CO_2$ emissions were higher.