• Journal of Physiology & Pathology in Korean Medicine
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• v.18 no.3
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• pp.740-746
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• 2004

In order to identify the anti-inflammatory and analgesic properties of natural herbal extracts, widely used in the Korean traditional medicine, an in vitro screening system was designed using pGL3, a luciferase reporter vector, and the tumor necrosis factor (TNF)-α and cyclooxygenase (COX)-II as target genes. The promoter regions of each gene was generated by PCR using the human chromosome as template DNA, and inserted into pGL3 vector with Kpnl and Hindlll. The final construct was transfected into human myleomonocytic leukemia cells (U937) that could be differentiated and activated by phorbol 12-myristate 13-acetate (PMA) or lipopolysaccharide (LPS). Using this system, we tested the anti-inflammatory and analgesic effects of several herbal extracts being regarded to have the medicinal effects of diminishing the body heat and complementing Qi. The well-known chemicals of PD98059 and berberine chloride were used as controls of the transcriptional inhibitors of TNF-α and COX-II, respectively. Among them, Salvia miltiorrhiza (Dan-Sam) was found to exhibit the significant medicinal properties of anti-inflammatory and analgesic effects.

• Korean Journal of Clinical Laboratory Science
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• v.37 no.2
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• pp.71-77
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• 2005

We experienced the specimen that contains a hemoglobin variant known as interference from HbAS in October 2003. It was the first case of Hb variants since Samsung Medical Center began conducting glycohemoglobin College of American Pathologists surveys in 1997. The purpose of this study is to share our experience with the specimen and promote the understanding of Hb variants & derivatives. We've performed cross checks to examine HbA1c by using two pieces of equipment; the TOSHOH G7 and BIO-RAD VARIANT-T(turbo), and Automatic High Performance Liquid Chromatography(HPLC) as an analytic measurement method. HPLC provides different fractional information of hemoglobin with a two-dimensional graph as well as numeric results. We have been performing a "Systematic Checking Process". Three specimen suspicious of Hb variants & derivatives were found through this process. College of American Pathologists notified that it is important for users to be aware of the limitation of their glycohemoglobin method to avoid reporting incorrect results due to interference from hemoglobin variants or hemoglobin adducts. Therefore, laboratory findings of Hb variants & derivatives are very important. The experience of qualified technicians with professional knowledge in Hb variants is the most important aspect in finding Hb variants. Korea is homogeneous in race and is not in an area with a higher finding rate of Hb variants. While 1,024 cases of Hb variants have been found in Japan, we do not have specific data on how many cases of Hb variants have been found in Korea. Considering Hb variant cases in Japan, which is geographically close to us, it is presumed that there must be various Hb variant cases in Korea. If domestic laboratories set a systemic protocol and build a network to share our experience in Hb variants, I expect the Korean Hb variants could also be listed on the world's Hb variant list.

• The KIPS Transactions:PartB
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• v.18B no.6
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• pp.341-348
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• 2011

ECG(Electrocardiogram), a field of Bio-signal, is generally experimented with classification algorithms most of which are SVM(Support Vector Machine), MLP(Multilayer Perceptron). But this study modified the Random Forest Algorithm along the basis of signal characteristics and comparatively analyzed the accuracies of modified algorithm with those of SVM and MLP to prove the ability of modified algorithm. The R-R interval extracted from ECG is used in this study and the results of established researches which experimented co-equal data are also comparatively analyzed. As a result, modified RF Classifier showed better consequences than SVM classifier, MLP classifier and other researches' results in accuracy category. The Band-pass filter is used to extract R-R interval in pre-processing stage. However, the Wavelet transform, median filter, and finite impulse response filter in addition to Band-pass filter are often used in experiment of ECG. After this study, selection of the filters efficiently deleting the baseline wandering in pre-processing stage and study of the methods correctly extracting the R-R interval are needed.

• Korean Journal of Environmental Agriculture
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• v.32 no.4
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• pp.260-267
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• 2013

BACKGROUND: In most studies, soil carbon sequestration has been evaluated simply with change of soil organic carbon content. So far, information regarding stability of soil organic carbon is limited. METHODS AND RESULTS: This study was conducted to determine changes in soil organic carbon (SOC) content and stability of carbon in response to compost application rates and plastic mulching treatment during the hot pepper growing season. Under the pot experiment condition, compost was mixed with an arable soil at rates corresponding to 0, 10, 20, and 40 Mg/ha. To determine effects of plastic mulching on soil carbon sequestration, plastic mulching and no mulching treatments were set up in soils amended with the application rate of 20 Mg/ha. The SOC content did not significantly increase with application of compost and plastic mulching at harvest time. No significant changes in bulk density with compost application and plastic mulching was found. These might result from short duration of experiment. While hot water extractable organic carbon content significantly decreased with compost application and plastic mulching, humic substances increased. Belowground biomass of hot pepper was biggest at the recommended application rate (20 Mg/ha) of compost. CONCLUSION: From the above results, continuous application of compost at the recommended application rate could improve increase in SOC content and stability of carbon in long term aspect.

• Journal of Life Science
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• v.22 no.7
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• pp.863-870
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• 2012

Rhodopsin, a dim light photoreceptor, has been regarded as one of the model systems for the structural and functional study of G protein-coupled receptors (GPCRs). Constitutively active mutant GPCRs leading to the activation of heterotrimeric GDP/GTP-binding protein signaling in the absence of ligand binding are of interest for the study of the activation mechanism in GPCRs. The present study focused on the opsin mutant E134Q/M257Y, which showed a moderate level of constitutive activity and the formation of two distinct rhodopsin chromophores with absorption maxima of 500 nm and 380 nm, depending on the presence of an inverse agonist, 11-cis-retinal, and an agonist, all-trans-retinal, respectively. Reconstitution of the mutant rhodopsin upon incubation with different ratios of 11-cis-retinal and the all-trans-retinal, as well as upon sequential binding of the two retinals, indicated its preferential binding to 11-cis-retinal. The thermal stability of the 11-cis-retinal-bound form of the E134Q/M257Y mutant was lower than that of the mutants containing a single replacement but higher than that of the all-trans-retinal-bound forms. The mutant also showed a lower stability in its opsin state as compared with that of the wild-type opsin but had little effects on the binding affinity to 11-cis-retinal. Information obtained in this study will be helpful for analyzing the structural changes associated with the activation of rhodopsin and GPCRs.

• Korean Journal of Environment and Ecology
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• v.28 no.1
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• pp.33-44
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• 2014

The purpose of this study was to provide basic information and investigate a vegetation structure around Tohamsan (Mt.) Wetland. Actual vegetation map was made on the basis of watershed around that. Vegetation structure survey was carried out for 8 representative communities of actual vegetation which were Pinus densifrora community, Quercus variabilis community, Cornus controversa community, Q. serrata community, Q. mongolica-Q. serrata community, Salix koreensis community, Q. mongolica community, Q. variabilis-Q. mongolica-Q. serrata community. Tohamsan (Mt.) Wetland is located on 490m above sea level and the area of watershed was $236,272m^2$. Vegetation type were divided into 16 types, and the ratio of Q. mongolica community was 33.1% ($78,209.2m^2$). In order to turn out the structure of 8 representative communties, 32 plots were set up and unit area of plot was $100m^2$. The estimated age of forest is 30~50-years-old, and in the resutls of soil analysis, acidity was pH 4.89 and organic matter was 4.46%.

• Journal of the Korean Academy of Child and Adolescent Psychiatry
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• v.1 no.1
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• pp.94-107
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• 1990

The survey of emotional behavioral problems in elementary school children provide baseline data for understanding, treating and preventing emotional behavioral problems in children. Since children can show different behaviors at home and at school, parents' information alone can lead to underestimate severity and prevalence rate of emotional behavioral problems in children. Thus, the author investigated the different the pattern of emotional behavioral problems in elementary school children by various socio-enviornmental factors in urban(Seoul) and rural area, by employing both parents' and teachers' questionnaire. The results were as follows ; Analysis of Rotter's questionnaire, in which high scores mean more serious emotional behavioral problems than low scores. The mean total problems scores for rural elementary school children were higher than those of Seoul elementary school children, and boys showed higher scores than girls. In relation to grade, mena total problems scores diminished as grade increased up to 4th or 5th, and then relatively high score for the 6th grade. Parent's education had significant effect on the total problem scores, that children of less educated parents showed higher score than children of more educated parents. Likewise the highest score was observed in children of unemployed parents. These results demonstrated that emotional behavioral children are related to various socio-environment factors.

• Journal of the Korean Society of Food Science and Nutrition
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• v.46 no.8
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• pp.1018-1025
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• 2017

The present study collected 18 kinds of commercially circulated liquid Sansuyu (Corni fructus) in pouch form and conducted comparative analysis on markings and physicochemical characteristics in order to utilize this information as a base material for the development of Sansuyu liquid products. The most abundant valid substance was morroniside, followed by loganin and cornin, in that order. Total concentration of valid substances in Cornus fruit products produced in the Gurye region was 168.50 mg/100 g, which was significantly higher than the overall average of 130.89 mg/100 g. There were seven (1st) and seven (2nd) kinds that contained more morroniside than the overall average, six (1st) and four (2nd) kinds with more loganin than the overall average, and eight (1st) and one (2nd) kinds with more cornin than the overall average. There were also five types in which no loganin was detected. There were significant differences in the valid substances of most products according to production period. Overall, these results show that processing method standardization research on extraction conditions is needed to produce liquid Sansuyu products of excellent quality.

• Journal of the Institute of Electronics Engineers of Korea SC
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• v.43 no.5 s.311
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• pp.89-100
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• 2006

The carotid intima-media thickness (IMT) is very important, because the severity of it is an independent predictor of transient cerebral ischemia, stroke, and coronary events such as myocardial infarction. The conventional image processing to measure the IMT has not been satisfactory, because the methods have relied on the manual section drawing and a regional segmentation by differential estimation. We propose a new image processing technology effective to extract features from the carotid artery image whose pixels have the directional vector properties with composed color distribution. The technique we presented here is not by differential variation but by verification of the layer properties of carotid artery image. Iterated vertical and horizontal analysis and segmentation of the IMT image show the vector characteristics. This new technique makes it possible to cluster the layers statistically, and to classify mathematical correlation between regions and resulting in correct measurements of thickness and its variation. The advantages and effectiveness of this approach are applicable to region process and character extraction of such a vector image.

• Journal of the Korean Society of Food Science and Nutrition
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• v.42 no.12
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• pp.1988-1993
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• 2013

The aims of this study were to investigate the quality characteristics of yellow layer cake added with Sparassis crispa powder that were abundant in dietary fiber, and also to determine the most suitable amount of added S. crispa powder. Dried powder of S. crispa containing 61.8 g/100 g insoluble dietary fiber was added to the cake batter in Baker's percentage of 1%, 2%, 4%, and 8%. Cake batter containing 1% and 2% S. crispa powder showed similar pH, specific gravity, and viscosity values to the batter without S. crispa powder (control). As the amount of added S. crispa power increased, the volume, specific volume, baking loss, and brightness (for both crust and crumb) of the cake containing the powder tended to decrease. A greater amount of added S. crispa powder resulted in a decrease in hardness, gumminess, and chewiness of the cake containing the powder and also reduced the changes in hardness of the cake during 8 days of storage. The sensory analysis showed that cake containing 2% S. crispa had several desirable kinds of sensory attributes, such as color, flavor, taste, and texture compared to the control; whereas an addition of more than 2% S. crispa deteriorated the sensory quality of the cake. These results suggest that the most suitable amount of added S. crispa powder for preparing yellow layer cake was 2% in Baker's percentage.