• Biomolecules & Therapeutics
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• 제25권2호
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• pp.165-170
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• 2017

Cordyceps bassiana is one of Cordyceps species with anti-oxidative, anti-cancer, anti-inflammatory, anti-diabetic, anti-obesity, anti-angiogenic, and anti-nociceptive activities. This mushroom has recently demonstrated to have an ability to reduce 2,4-dinitrofluorobenzene-induced atopic dermatitis symptoms in NC/Nga mice. In this study, we further examined phytochemical properties of this mushroom by column chromatography and HPLC analysis. By chromatographic separation and spectroscopic analysis, 8 compounds, such as 1,9-dimethylguanine (1), adenosine (2), uridine (3), nicotinamide (4), 3-methyluracil (5), 1,7-dimethylxanthine (6), nudifloric acid (7), and mannitol (8) were identified from 6 different fractions and 4 more subfractions. Through evaluation of their anti-inflammatory activities using reporter gene assay and mRNA analysis, compound 1 was found to block luciferase activity induced by $NF-{\kappa}B$ and AP-1, suppress the mRNA levels of cyclooxygenase (COX)-2 and tumor necrosis factor $(TNF)-{\alpha}$. Therefore, our data strongly suggests that compound 1 acts as one of major principles in Cordyceps bassiana with anti-inflammatory and anti-atopic dermatitis activities.

• Journal of the Korean Wood Science and Technology
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• 제49권4호
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• pp.336-359
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• 2021

본 연구는 고층 목조건축에 사용되는 대형 목재 패널인 CLT의 낮은 rolling shear strength를 개선하기 위해 hybrid CLT의 연구 개발 동향을 분석하였다. 이를 통해 CLT의 국산화를 위한 연구개발 방향에 활용 가능한 기초자료를 마련하고자 하였고, 낮은 rolling shear strength를 향상시키기 위한 방안으로 활엽수 층재 사용, 층재 배열 각도 변화, 구조용 목질복합체의 사용이 주를 이루고 있다. 활엽수 층재는 침엽수 보다 rolling shear strength와 shear modulus 모두 2배 이상의 높은 값을 나타내므로 활엽수 층재 사용 및 미이용 수종의 활용이 가능함을 확인하였다. 층재 배열 각도 변화에 따라 rolling shear stength 1.5배, shear modulus 8.3배, bending stiffness 4.1배 향상되어 층재 배열 각도를 감소시킴으로써 CLT 강도 향상을 확인하였다. 구조용 목질재료는 기존에 강도성능이 확보된 재료로 층재로 사용하였을 때 최대 MOR 1.35배, MOE 1.5배, rolling shear strength 2배 향상되었고, 층재 간의 접착강도 또한 집성재 블록전단강도 기준인 7.0 N/mm²을 확보할 수 있었다. 선행연구결과를 통해 MOE 7.0 GPa, MOR 40.0 MPa 이상의 휨 특성을 가진 구조용 목질재료를 사용하였을 때 강도성능이 향상됨을 확인하였다. 이를 통해 구조용 목질재료 층재 강도 기준으로 판단하였다. rolling shear strength 개선하기 위한 최적의 방법은 기존 규격에 의한 강도값을 가진 구조용 목질재료의 적용이 가장 유리할 것으로 판단하나, 구조용 목질재료의 섬유 배열에 따른 CLT 층재 배열 방향, 층재 간 접착 강도 등에 대한 추가적인 연구가 필요하다.

• 농업과학연구
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• 제12권1호
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• pp.139-152
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• 1985

본 NEWASA 지역의 접수관정관개 개발에 따른 정제성분석 연구는 IBRD-EDI와 INDIA RESERVE BANK의 College of Banking이 공동주최한 Trainers Trining for Rural Finance (1985. 1.19-3.1)의 일환으로 1985. 2. 14-19 까지 NEWASA BLOCK에 대한 정수정관개의 경제적 효율성을 평가하기 위하여 농가조사를 실시하였고, 그 분석결과를 정리한 것으로 농지는 넓으나 농업용수가 부족한 본 지역은 빈곤과 저생산성 농촌잠재 실업을 특징으로 하고 있다. 농촌소득의 제고와 농촌실업의 고용확대는 전 인구의 80% 이상이 농민인 본지역뿐만 아니라 선언도 경제개발의 중심과제이다. 호당 6 acres 규모의 관개가 가능한 Dug-well Irrigation Development는 연평균 강우량이 650m/m에 불과한 인도의 Plateau 지역에서는 농촌빈곤과 실업문제의 해결, 토지 이용율제고를 통한 토지생산성 향상등 농촌경제개발의 중심과제로서 인도정부가 추진하는 핵심적인 농업정책이다. 본 지역의 농가호당 평균 소유농지연적은 8.67 acres(3.5ha)로써 모두가 Rainfed farming으로 현재 본 지역의 관개율은 17.5%에 불과하다. 농작물은 Jowar라고 하는 내한성 작물이 재배되며, 관개 조건하에서는 Jowar, Wheat, Sugarcane이 재배된다. With and Without Dug-Well Irrigation 조건하에서 각 작물별 ha당 생산성을 보면 Jowar는 0.86 ton/ha에서 2.22 ton/ha으로 증산되고 Wheat는 2.96 ton/ha, Sugarcane는 98.8 ton/ha이 생산된다. 한편 Dug-well Irrigation 전후의 농가소득의 변화를 보면 시행전의 4,275Rs가 되어 4배의 농가소득이 증가된다. 이와 같은 농업소득을 획득하기 위해서는 Dug-well에 대한 Initial Investment로서 33,400Rs(2,137,600원)이 투자되어야 하는데 종류별 융자 기간을 보면 Dug-well 15년(95%융자) Pump Set 9년(95%융자), Woriking capital 1년이며 인도의 Union Bank에서 융자하고 있다. 결론적으로 이러한 Dug-well Irrigation Development에 따른 IRR는 Before Financing시에는 29%이며 After Financing 시에는 24%이고 B/C ratio는 두경우에 있어 1.89, 1.24를 각각 나타냈다. 따라서, 본 사업은 재무적인 측면 즉 농가소득증대와 농촌고용증대 및 토지이용면에서 타당성이 크며 Dug-well Irrigation은 인도의 농업성장 및 경제개발의 관건으로 생각된다. 한국의 수리개발 기술 및 농업기술(Bio-chemical, Mechanical & Hydrological Technology)이 이전된다면 보다 괄목할 만한 농업발전이 이룩될 것을 생각된다.

• 대한방사선기술학회지:방사선기술과학
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• 제38권4호
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• pp.451-461
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• 2015

MV방사선 치료는 둘러싸여 있는 정상조직의 피폭선량을 최소화 하면서, target volume 내에 정확하게 선량을 전달하는데 있어 중요한 요인이다. 본 연구에서는 방사선 치료의 높은 정확성을 유지하기 위하여 megavoltage X-ray imaging (MVI)에서 edge block 을 사용한 digital radiography (DR) system 검출기의 modulation transfer function (MTF: 변조전달함수), the noise power spectrum (NPS: 잡음전력스펙트럼) and the detective quantum efficiency (DQE: 양자검출효율)를 측정하고자 한다. 우리는 텅스텐으로 구성된 19 (thickness) ${\times}$ 10 (length) ${\times}$ 1 (width) $cm^3$의 edge block을 사용하였으며, 다음과 같은 setting들로 pre-sampling modulation transfer function (MTF)를 계산하였다: 6-megavolt (MV) energy를 사용하고, 다양한 Radiotherapy장비인 TrueBeamTM (Varian), BEAMVIEWPLUS (Siemens), iViewGT (Elekta), ClinacR iX (Varian) 를 사용하였다. MTF결과에서 Varian TrueBeamTM flattening filter free가 MTF의 50% ($mm^{-1}$)에서 0.46, 10% ($mm^{-1}$) 에서 1.40로 가장 highest value를 보였다. Noise 분포는 Elekta iViewGT가 가장 낮은 분포를 보였다. DQE에서는 E lekt a iViewGT가 peak DQE에서 0.0026 그리고 $1mm^{-1}$ DQE 에서 0.00014로 가장 높았다. 본 연구는 Edge method를 이용하여 MTF와 DQE산출을 재현하였으며, 현재 임상에서 사용되는 DR 시스템 측정의 높은 정확성을 유지할 수 있었으며 이러한 연구는 전통적인 QA 영상화뿐만 아니라 검출기 개발 연구에 있어서 정량적인 MTF, NPS, DQE 측정에 더욱 더 효율적으로 사용될 수 있다는 것을 알 수 있다.

• Asian-Australasian Journal of Animal Sciences
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• 제17권4호
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• pp.523-532
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• 2004

An experiment was conducted to investigate the effect of microbial phytase ($Natuphos^{R}$) supplementation in combination with enzyme complex (composed of enzymes targeted to SBM dietary components such as $\alpha$-galactosides and galactomannans; $Endo-Power^{R}$) to diet with low nutrient levels on growth performance and ileal nutrient digestibility of weaned pigs. A total of 210 crossbred weaned pigs (Landrace$\times$Yorkshire$\times$Duroc), 6.68$\pm$0.98 kg of initial body weight, were randomly allotted to five dietary treatments, based on weight and age, according to a randomized complete block design. There were three pens per treatment and 14 pigs per pen. The dietary treatments were 1) CON (Control diet with no phytase and enzyme complex (EC)), 2) LP+EC 100 (Control diet with 0.15% unit lower available phosphorus (aP) level+0.1% phytase (500 FTU/kg diet) and 0.1% enzyme complex), 3) LP+EC 80 (Control diet with 0.15% unit lower aP level+0.08% phytase (400 FTU/kg diet) and 0.08% enzyme complex, 4) LPEA+EC 100 (Control diet with 0.15% unit lower aP and 3% lower ME and amino acid levels (lysine, methionine, threonine and typtophan)+0.1% phytase (500 FTU/kg diet) and 0.1% enzyme complex), 5) LPEA+EC 80 (Control diet with 0.15% unit lower aP and 3% lower ME and amino acid levels+0.08% phytase (400 FTU/ kg diet) and 0.08% enzyme complex). For the determination of ileal nutrients digestibility, a total of 15 T-cannulated pigs (initial body weight; 7.52$\pm$1.24 kg; 3 replicates per treatment) were used in the present study. Piglets were weighted and allotted into same dietary treatments as one in growth trial and phase I experimental diets were provided for ileal digestibility study. There was no significant difference (p>0.05) in average daily gain (ADG) and average daily feed intake (ADFI) among dietary treatments during the whole experimental period (0 to 5 weeks). However, piglets in LP+EC 100 group had a significantly higher gain/feed ratio (G:F) than piglets had in control (p<0.05). Crude protein, energy and phosphorus digestibilities were significantly improved when both of phytase and enzyme complex were supplemented at the revel of 0.1%, respectively to diets with low nutrient level (aP or (and) ME and amino acids) (p<0.05). Piglets in LP+EC 100 and LPEA+EC 100 groups showed significantly higher phosphorus content (%) in bone than that of piglets in control group (p<0.05). Supplementation of both of phytase and enzyme complex at 0.1%, respectively, to diet with low nutrient levels (aP or (and) ME and amino acids) significantly improved total ileal essential amino acid and nonessential amino acid digestibilities compared to control group (p<0.05). In conclusion, the results from the present study suggest that the simultaneous inclusion of phytase and enzyme complex to diets at recommended level is advantageous with respect to improving growth performance and nutrient digestibility of weaned pigs and may contribute to increased economic return when added to corn-soy based weaned pig diets.

• 한국초지조사료학회지
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• 제25권1호
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• pp.1-6
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• 2005

With recent interest organic farming the use of legumes including vetch and clover to provide N to adjacent crops is increasing in Korea. In the present studies, we conducted a trial to investigate the effects of the application of N rate on nitrogen fixation and transfer from vetch to barley in mixed stands. The experiment was arranged in a randomized complete block design with three replications. Four different N rates(0, 75, 113 and 150/ha) was used and vetch+barley was broadcasted manually on 1.5 $\times$2 m plot in Oct. 2001. Half of urea and K$_{2}O, 200 Phosphate and 75 kg potash per ha were applied as basal dressing md half of N md 75 potash were used for topdressing to soil surface on MarctL 2002. The equivalent of 1kg ha$^{-1}$ at($^{15}$NH$_{4}$)$_{2}$SO$_{4}$ solution at 99.8 atom $\%$$^{15}$N excess was applied to the microplot in mid April. Forage was harvested from each plot at ground level and separated into barley and vetch. Total N content and It values of samples were determined using a continuous flow stable isotope ratio mass spectrometry(IsoPrime-EA. Micromass, UK.). The percentage of legume H fixed from atmospheric N2 were 95.0, 93.8, 94.4 and $84.8\%$ with increment of N levels. The percentage of N transfer from vetch to barley by N-difference method with increment of N fertilizer were from 58 to$49\%$ while 39 to $23\%$ in $^{15}$N-dilution method. The amount of transfer from vetch to barley were 87 to 68 kg/ ha with N level by N-difference moth여 and 58 to -56/ha with N application levels by $^{15}$N dilution method. The amount of nitrogen fixation per ha were from 150 kg / ha to 219 kg / ha by different method, but on the other side 49 to 105kg/ha by N$^{15}$-dilution.

• 산업식품공학
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• 제21권2호
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• pp.103-109
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• 2017

DEAE sephacel anion chromatography 및 SP sepharose cation chromatography에 의해 Streptomyces violaceoruber 유래 alginate lyase의 정제를 수행하여 비활성 14.59 units/mL 정제배율 40.64배를 나타내었다. Tricine SDS-PAGE에 의한 단일밴드를 확인하였고, 분자량은 23.3 kDa으로 결정되었다. 정제효소에 의해 sodium alginate를 가수분해하여 1차 activated carbon column chromatography와 2차 bio gel P-2 gel filtration에 의해 당가수분해물을 분리 회수하여 TLC와 FACE를 통해 중합도를 확인하고 Timell's method에 의해 hetero type M/G-oligosaccharide 중합도 6, 8로 결정되었다. B. animalis, B. bifidum, B. breve, B. infantis, B. longum와 L. acidophilus, L. casei, L. reuteri에 생육활성에 대한 중합도 6, 8의 영향을 검토하기 위하여 modified-MRS media에 탄소원으로 중합도 6, 8를 대체하여 생육활성을 비교한 결과 B. longum에서는 D.P. 6 M/G-oligosaccharide를 탄소원으로 대체한 경우 표준 MRS배지와 비교하여 4.25배, D.P. 8에서 6.44배의 상대활성을 나타내어 가장 우수한 생육활성을 나타내었으며, B. bifidum의 경우에서도 D.P. 6에서 3.27배, D.P. 8에서 5.4배의 상대활성을 나타내었다. 이외에도 B. animalis, B. breve그리고 L. casei에 있어서도 D.P. 8의 경우 3배의 상대활성을 나타내었으나, L. reuteri에 대한 D.P. 8의 경우에서는 표준 MRS media와 비교하여 0.29배로 감소하였다. 결과적으로 D.P. 8의 올리고당이 D.P. 6의 올리고당보다 생육활성에 크게 기여하는 것으로 나타났다.

• 농업과학연구
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• 제43권1호
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• pp.52-60
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• 2016

In animal reproduction, the quality of oocytes and embryos has been evaluated by the expression of specific molecules. Follistatin (FST), which was isolated from follicular fluid, binds and bio-neutralizes the TGF-${\beta}$ superfamily members. Previous studies using the bovine model showed FST could be an important molecular determinant of embryo developmental competence. However, the effect of FST treatment on porcine embryo developmental competence has not been established. In this study, the effect of exogenous FST on porcine embryo developmental competence was investigated during in vitro culture. FST (10 ng/ml) treatment induced a significant decrease in the rate of cell arrest at the 4-cell stage. The expression levels of DNA-methyltransferase 1 (DNMT1), histone deacetylase 1 (HDAC1), and histone deacetylase 2 (HDAC2) were decreased in 4-cell stage embryos. FST treatment also resulted in significant improvements in developmental competence of embryos in terms of blastocyst formation rate and OCT-4 mRNA levels, the latter being related to pluripotency. In conclusion, during in vitro culture, FST treatment significantly ameliorated 4-cell block during embryonic development and improved embryo developmental competence. Therefore, FST treatment may potentially have a functional role in porcine embryogenesis that is broadly applicable to enhance in vitro embryo development.

• 동의생리병리학회지
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• 제31권2호
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• pp.105-110
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• 2017

Prolonged exposure to solar ultraviolet A (UVA) radiation has been known to cause premature skin aging (photo-aging). UVA radiation generates ROS thereby induce degenerative changes of skin such as degradation of dermal collagen, elastic fibers. Matrix metalloproteinases (MMPs), the proteolytic enzymes have been implicated as a major player in the development of UVA-induced photo-aging. Many studies have been conducted to block the harmful effects of UV radiation on the skin. Recently, we are interested in the availability of fermented red ginseng (FRG) as natural matrix metalloproteinases inhibitors (MMPIs). The efficacy difference between red ginseng and FRG has been compared. Both RG and FRG have no cytotoxic effects below the concentration of $300{\mu}g/ml$. Human dermal fibroblasts (HDFs) were pretreated with FRG or RG for 24h, followed by irradiation of UVA. Then, we measured the intracellular ROS production and the expression of MMP, $IL-1{\beta}$ at the mRNA level. We also examined the intracellular localization of $NF-{\kappa}B$ and MMP-9 on the FRG or RG treated and UVA-irradiated HDFs. FRG decreased the intracellular ROS production elicited by UVA. In addition, FRG decreased the mRNA expression of MMP-3, MMP-9, and $IL-1{\beta}$ more efficiently than RG. Furthermore, FRG suppressed the nuclear localization of $NF-{\kappa}B$, and the expression of MMP-9. Taken together, our results suggest that FRG is promising agents to prevent UVA-induced photo-aging by suppressing MMP expression and inflammation.

• Asian-Australasian Journal of Animal Sciences
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• 제17권8호
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• pp.1150-1157
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• 2004

This experiment was conducted to investigate effects of phase feeding and sugar beet pulp (SBP) on growth performance, nutrient digestibility, nitrogen excretion, blood urea nitrogen (BUN) concentration and carcass characteristics in finishing pigs. A total of 128 pigs were allotted at 53.9 kg BW to 8 replicates in a 2$\times$2 factorial arrangement in a randomized complete block (RCB) design. The first factor was phase feeding (2 or 3 phase feeding) and SBP (SBP: 0% or 10%) was the second factor. Ten percent SBP supplement groups showed lower average daily feed intake (ADFI) than 0% SBP supplement groups (p<0.05). However, there were no significant difference in average daily gain (ADG) and feed:gain ratio among treatments during overall experimental period. Nutrient digestibility was not affected by phase feeding or SBP supplementation. Urinary nitrogen excretion in 10% SBP supplement group was lower than that in 0% SBP supplement group (p<0.05) and total nitrogen excretion was lower in SBP supplement group than in the group without SBP. Urinary and total nitrogen were numerically decreased in three phase feeding compared to two phase feeding. The BUN concentration in three phase feeding groups was lower than two phase feeding groups at 47 and 63 day (p<0.05). Consequently, results of this experiment demonstrated that three phase feeding was more acceptable than two phase feeding for finishing pigs. And sugar beet pulp could be supplemented in finishing pig diet for decreasing urinary nitrogen excretion without retardation in growth performance of pigs.