• Animal cells and systems
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• 제1권4호
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• pp.589-594
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• 1997

The neuronal voltage-sensitive calcium channels (VSCCs) are multisubunit complexes consisting of $\alpha_1,\;\alpha_2-\delta$ and $\beta$ subunits. Heterologous expression and biochemical studies have shown that the activity of VSCCs is regulated by their $\beta$ subunits in a $\beta$ subunit isoform-specific manner. To elucidate the $\beta$ subunit identity of the P/Q-type calcium channel encoded by an $\alpha_{1A}$ subunit, which is exclusively expressed in the Purkinje and granule cell of the cerebellum, we have examined the spatial and temporal expression patterns of $\beta$ subunits and compared them with those of $\alpha_{1A}$ subunit in the developing rat cerebellum. Reverse transcriptase- polymerase chain reaction (RT-PCR) and Northern blot analysis have shown that $\beta_4$ subunit mRNA was prominently expressed in the cerebellum and much more abundant than any other distinct $\beta$ subunits. RNase protection assay has further demonstrated that the expression of $\alpha_{1A}$ and $\beta_4$ subunits increased during cerebellar development, while the amount of $\beta_2$ and $\beta_3$ mRNAs did not significantly change. In addition, a $\beta_4$ transcript was present in cultured cerebellar granule cells, but not in astrocyte cells, and the level of $\beta_4$ mRNA expression increased gradually in vitro seen as in vivo. Based on the spatial and temporal expression patterns of $\beta_4$ subunit, we conclude that $\beta_4$ may predominantly associate, but probably not exclusively, with the $\alpha_{1A}$ subunit in rat cerebellar granule cells.

• The Plant Pathology Journal
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• 제35권1호
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• pp.11-18
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• 2019

Gaeumannomyces graminis var. tritici is a soil borne pathogenic fungus associated with wheat roots. The accurate quantification of gene expression during the process of infection might be helpful to understand the pathogenic molecular mechanism. However, this method requires suitable reference genes for transcript normalization. In this study, nine candidate reference genes were chosen, and the specificity of the primers were investigated by melting curves of PCR products. The expression stability of these nine candidates was determined with three programs-geNorm, Norm Finder, and Best Keeper. $TUB{\beta}$ was identified as the most stable reference gene. Furthermore, the exopolygalacturonase gene (ExoPG) was selected to verify the reliability of $TUB{\beta}$ expression. The expression profile of ExoPG assessed using $TUB{\beta}$ agreed with the results of digital gene expression analysis by RNA-Seq. This study is the first systematic exploration of the optimal reference genes in the infection process of Gaeumannomyces graminis var. tritici.

• Journal of the Korean Statistical Society
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• 제26권3호
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• pp.309-317
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• 1997

A sequential procedure for estimating a linear function of two normal means which satisfies the two requirements, i.e. one is a condition of coverage probability, the other is a condition of $\beta$-protection, is proposed when the variances are unknown and not necessarily equal. We give asymptotic behaviors of the proposed stopping time.

• 충청수학회지
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• 제5권1호
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• pp.87-97
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• 1992

Sequential procedure with ${\beta}$-protection for the mean vector ${\mu}(\theta)$ of a p(> 1)-variate multivariate distribution $P_{\theta}$, ${\theta}{\in}{\Theta}$, with covariance matrix ${\sum}(\theta)$ is considered when the only nuisance parameters is ${\sum}(\theta)$. We obtain a confidence set for ${\mu}(\theta)$ with coverage probability condition and ${\beta}$-protection at ${\mu}-{\delta}(\mu)$ for some imprecision function ${\delta}:\mathbb{R}^p{\rightarrow}\mathbb{R}^p$.

• Journal of Radiation Protection and Research
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• 제26권3호
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• pp.149-154
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• 2001

Calculational models to evaluate radiation doses resulting from the medical use of high energy beta-ray sources are presented. The radioactive sources considered are Sr-90/Y-90 used as ophthalmic applicator, Re-188 used for treating restenosis of coronary artery, and Ho-166 used for treating hepatic tumors. Typical therapeutic situations which might induce relatively high radiation doses the medical person involved were considered to compute by using MCNP-4C Monte Carlo code the radiation doses. The calculation results suggest that for all of the cases considered, the evaluated radiation doses are negligible compared to the dose limits. It is also found that the effect of Bremsstrahlung radiations on the total dose is insignificant, and hence the conventional lead gown is also effective in shielding beta-rays.

• 한국간호교육학회지
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• 제22권1호
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• pp.83-95
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• 2016

Purpose: This study aims to identify perception, performance, and the related factors of performance in regards to patients' medical information protection among allied health college students. Methods: Four hundred twelve subjects from three colleges located in Jeonbuk and Kyungbuk province consented to participate. Data was collected from November 28 to December 15, 2012. To assess perception and performance in regards to patients' medical information protection, a self-reporting questionnaire was used. Data was analyzed via SPSS 18.0 program. Results: The score of perception and performance about patients' medical information protection were 4.07 and 3.56, respectively. All item's scores of performance were significantly lower than those of perception. The perception score was significantly different according to recognition of hospital ethics code (t=1.95, p=.052), and recognition of association ethics code (t=2.88, p=.004). The performance score was significantly different according to gender (t=-3.32, p=001), major (F=14.41, p<.001), clinical practicum hospitals (F=8.22, p<.001), and method of electronic medical record access (F=3.23, p=.023). The factors influencing performance were perception(${\beta}=.46$, p<.001), duration of clinical practice(${\beta}=-.36$, p<.001), and gender(${\beta}=.09$, p=.033). Conclusion: In order to improve performance in regards to patients' medical information protection of allied health college students, we should develop ethical education programs and standardize them through multidisciplinary collaboration.

• 대한화장품학회지
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• 제13권1호
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• pp.45-71
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• 1987

${\beta}$-Carotene has been known as an effective quenching agent of singlet oxygen and the carotenoid pigments in general are expected to protect cells against photosensitized oxidations. We are determined the quenching rate constants of several Ketocarotenoids including capsanthin, capsanthin diester, astaxanthin and fucoxanthin, and the relative quenching actiyities against singlet oxygen were compared with those of ${\beta}$-carotene and reported carotenoids. Nevertheless the ketocarotenoids exhibited lower quenching rate constants than ${\beta}$-carotene, they showed more pronounced protective activitives than ${\beta}$-carotene against photohemlysis induced by singlet oxygen. Among the ketocarotenoids investigated, fucoxanthin indicated a significant protective activity for the cell. The results suggested that. 1) 1O2 may be alikely initiator of photohemolysis, but this reaction is followed by slow dark reactions involving secondary reactive species. 2) For protection of RBC against photodynamic action with carotenoids, carotenoids having functional groups such as -C=0 and -OH groups are most efficient. This suggests that partition of carotenoids between the buck and the mombrane and/or their specific binding to membrane proteins are more critical for the photo-protection by carotenoids than is a diffusional quenching of 1O2.

• Food Science and Biotechnology
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• 제17권1호
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• pp.199-202
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• 2008

The effects of $\beta$-(1,6)-galactosyl trehalose trisaccharide ($\beta$-GT) that was preferentially produced by Escherichia coli $\beta$-galactosidase on cryo- and thermo-protections of protein were investigated with those of other sugars at the level of 8% (w/v). As compared to a control without sugar additive, $\beta$-GT effectively enhanced 32-54% of the cryoprotection of fish actomyosin against repeated freeze-thawing and frozen storage, and also 49% of the protection against thermal inactivation of pyrophosphatase, respectively. As a result, it was significantly more effective than sorbitol and trehalose in both cryoprotection and thermoprotection. Thus, $\beta$-GT would be possibly applied as a sugar substitute for cryo- and thermo-protective applications of food protein.

• Journal of Radiation Protection and Research
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• 제43권4호
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• pp.154-159
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• 2018

Background: To prevent small leakage accidents, a real-time and direct detection system for small leaks with a detection limit below that of existing systems, e.g. $0.5gpm{\cdot}hr^{-1}$, is required. In this study, a small-size beta detector, which can be installed inside the reactor containment (CT) building and detect small leaks directly, was suggested and its feasibility was evaluated using MCNPX simulation. Materials and Methods: A target nuclide was selected through analysis of radiation from radionuclides in the reactor coolant system (RCS) and the spectrum was obtained via a silicon detector simulated in MCNPX. A window was designed to reduce the background signal caused by other nuclides. The sensitivity of the detector was also estimated, and its shielding designed for installation inside the reactor CT. Results and Discussion: The beta and gamma spectrum of the silicon detector showed a negligible gamma signal but it also contained an undesired peak at 0.22 MeV due to other nuclides, not the $^{16}N$ target nuclide. Window to remove the peak was derived as 0.4 mm for beryllium. The sensitivity of silicon beta detector with a beryllium window of 1.7 mm thickness was derived as $5.172{\times}10^{-6}{\mu}Ci{\cdot}cc^{-1}$. In addition, the specification of the shielding was evaluated through simulations, and the results showed that the integrity of the silicon detector can be maintained with lead shielding of 3 cm (<15 kg). This is a very small amount compared to the specifications of the lead shielding (600 kg) required for installation of $^{16}N$ gamma detector in inside reactor CT, it was determined that beta detector would have a distinct advantage in terms of miniaturization. Conclusion: The feasibility of the beta detector was evaluated for installation inside the reactor CT to detect small leaks below $0.5gpm{\cdot}hr^{-1}$. In future, the design will be optimized on specific data.

• BMB Reports
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• 제39권3호
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• pp.311-318
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• 2006

One of the biggest group of proteins influenced by protein kinase CK2 is formed by factors engaged in gene expression. Here we have reported recently identified yeast transcription elongation factor Elf1 as a new substrate for both monomeric and tetrameric forms of CK2. Elf1 serves as a substrate for both the recombinant CK2$\alpha$' ($K_m$ 0.38 ${\mu}M$) and holoenzyme ($K_m$ $0.13\;{\mu}M$). By MALDI-MS we identified the two serine residues at positions 95 and 117 as the most probable in vitro phosphorylation sites. Co-immunoprecypitation experiments show that Elf1 interacts with catalytic ($\alpha$ and $\alpha$') as well as regulatory ($\beta$ and $\beta$') subunits of CK2. These data may help to elucidate the role of protein kinase CK2 and Elf1 in the regulation of transcription elongation.