• Korean Journal of Pharmacognosy
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• v.46 no.4
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• pp.327-333
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• 2015

Alzheimer's disease (AD) is a progressive neurodegenerative disorder symptomatically characterized by impairment in memory and cognitive abilities. AD is characterized pathologically by the deposition of ${\beta}$-amyloid ($A{\beta}$) peptides of 40-42 residues, which are generated by processing of amyloid precursor protein (APP). $A{\beta}$ has been believed to be neurotoxic and now is also considered to have a role on the mechanism of memory dysfunction. In this study, we tested that EtOH extract of the fruits of Chaenomeles sinensis Koehne (CSE) affects on the processing of APP from the APPswe over-expressing Neuro2a cell line. We found that CSE increased over 2 folds of the $sAPP{\alpha}$ secretion level, a metabolite of ${\alpha}$-secretase. We showed that CSE reduced the secretion level of $A{\beta}42$ and $A{\beta}40$ by down regulation of ${\beta}$-secretase (BACE) without cytotoxicity. Furthermore, we found that CSE inhibited BACE and acetylcholinesterase activity in vitro. We suggest that Chaenomelis Fructus may be an useful source to develop a herbal medicine for AD.

• Journal of Microbiology and Biotechnology
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• v.5 no.1
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• pp.6-13
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• 1995

A gene coding for the $\beta$-galactosidase (lactase) of Kluyveromyces tragilis UCD 55-55 was isolated by complementation in Escherichia coli YMC9. From the plasmid library made from Sau3A-digested chromosomal DNA, one positive clone was selected. The cloned gene for $\beta$-galactosidase was on 7.3 kilobase pair DNA fragment, and a slightly low level of $\beta$-galactosidase enzyme activity was detecied in E. coli. It was also confirmed that the cloned gene comes from K. tragilis by DNA-DNA hybridization and immunochemical blotting experiments. In order to construct a new yeast strain having the metabolic ability for lactose, the cloned gene for K. tragilis $\beta$-galactosidase was inserted in yeast vector YEp24 and YRp17, and transformed into Saccharomyces cerevisiae YNN27 and Ml-2B. The yeast transformants showed the nearly the same $\beta$-galactosidase productivity as level of K. tragilis when uninduced, but these could not utilize lactose as a sole carbon source, presumably due to the lack of lactose transport system. Nevertheless, a slightly higher ethanol productivity was achieved by these transformants than S. cerevisiae or K. tragilis, in the medium containing glucose and lactose.

• Fisheries and Aquatic Sciences
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• v.19 no.2
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• pp.11.1-11.7
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• 2016

In this study, purified peptides from shrimp waste hydrolysates (SWHs) were examined for their inhibitory effects against ${\beta}$-secretase. During consecutive purification using a Sephadex G-25 column chromatography and high performance liquid chromatography on a C18 column, a potent ${\beta}$-secretase inhibitory peptide Asp-Val-Leu-Phe-His (629 Da) was isolated and identified from SWH24 by Q-TOF MS/MS and the $IC_{50}$ value was determined to be $92.70{\mu}M$. The ${\beta}$-secretase inhibition patterns of the purified peptides were found to be competitive. Among synthesized ${\beta}$-secretase inhibitory peptides, Leu-Phe-His had higher ${\beta}$-secretase inhibitory activity than the others. The result of this study suggests that the ${\beta}$-secretase inhibitory peptide derived from SWH24 could be potential candidates to develop nutraceuticals and pharmaceuticals.

• Korean Chemical Engineering Research
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• v.54 no.5
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• pp.719-721
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• 2016

Formate has been considered as an environmentally sustainable feedstock that can be used to accelerate the production of valuable chemicals. This study presents brief results of the formatotrophic production of Poly-${\beta}$-hydroxybutyric acid (PHB) by Methylobacterium sp. To evaluate the production of PHB, five species of Methylobacteria were tested using formate as the sole carbon and energy source. Methylobacterium chloromethanicum CM4 exhibited the highest productivity of PHB, which showed 1.72 g/L PHB production, 32.4% PHB content, and 0.027 g-PHB/g-formate PHB yield. These results could be used for the formatotrophic production of PHB with the concurrent reduction of $CO_2$ to formate.

• Communications of the Korean Mathematical Society
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• v.23 no.4
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• pp.529-540
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• 2008

This paper deals the global existence and blow-up properties of the following non-Newton polytropic filtration system, $${u_t}-{\triangle}_{m,p}u=u^{{\alpha}_1}\;{\int}_{\Omega}\;{\upsilon}^{{\beta}_1}\;(x,\;t)dx,\;{\upsilon}_t-{\triangle}_{n,p}{\upsilon}={\upsilon}^{{\alpha}_2}\;{\int}_{\Omega}\;u^{{\beta}_2}\;(x,{\;}t)dx,$$ with homogeneous Dirichlet boundary condition. Under appropriate hypotheses, we prove that the solution either exists globally or blows up in finite time depends on the initial data and the relations of the parameters in the system.

• KSBB Journal
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• v.13 no.3
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• pp.325-330
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• 1998

The methylotrophic yeast, Pichia pastoris, is known to be a potential host to offer many advantages for production of recombinant proteins. Fermentation parameters were optimized to enhance the heterologous ${\beta}$-galactosidase productivity with P. pastoris. Optimum concentration of methanol, used as inducer, was observed to be 8 g/L and the extent of repression of AOX1 promoter by glycerol was lower than by glucose. The degradation of the gene product ${\beta}$-galactosidase by protease was inhibited as the pH increased from 5 to 8 and the yeast extract(1%) as nitrogen source increased expression level 4 times higher compared to yeast nitrogen base(1%) as nitrogen source increased expression level 4 times higher compared to yeast nitrogen base(1%). Induction method, in which methanol is just added to fermentation medium without centrifugation, was found to be as much effective as the one with centrifugation.

• Proceedings of the IEEK Conference
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• 2008.06a
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• pp.585-586
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• 2008

In this paper, a novel beta-multiplier reference current source circuit for the 800mV power-supply voltage is presented. In order to cope with the narrow input common-mode range of the OpAmp in the reference circuit, shunt resistive voltage divider branches were deployed. High gain OpAmp was designed to compensate intrinsic low output resistance of the MOS transistors. The proposed reference circuit was designed in a standard 0.18um CMOS process with nominal Vth of 420mV and -450mV for nMOS and pMOS transistor respectively. The total power consumption including OpAmp is less than 50uW.

• The Bulletin of The Korean Astronomical Society
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• v.35 no.1
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• pp.41.2-41.2
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• 2010

The infrared astronomy satellite AKARI/FIS (Far-Infrared Surveyor) performed all-sky survey in 4 Far-IR bands (50 - 200${\mu}m$ range). It observed around 94% of the whole sky twice or more with a higher spatial resolution and a wider wavelength coverage than that of previous all-sky mission, IRAS. The AKARI/FIS bright source catalogue ${\beta}-2$ is the second released version which included around 290,000 point sources. It provides us with more reliable position and flux information, and around 4 times larger number of sources compared with the first version of catalogue. The sensitivity limit at $90{\mu}m$ band is 0.6 Jy and the estimated flux uncertainty is 20-35% for bright sources. The catalogue will be open to public in this year after the improvement of accuracy, reliability and completeness.

• Proceedings of the Korea Committee for Ocean Resources and Engineering Conference
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• 2000.10a
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• pp.63-67
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• 2000

Up to this day, Most studies of hydroelasticity are inclined to frequency domain atnlysis. Thos amlysis Q the landing, take-4, and dropping of airaqft on a structure. So, the concern of this prrper is a tra a VLFS subjected to dymmic lazd induced by airplane larndirrg and take-off. To predict added mass, dampr exciting force, the source-dipole distribution method were used The responses are accomplished by Fdoimain analysis method is based on Newmark $\beta$ method to pursuit time step pnzcedure taking advantage function for hvdrodvnumic effects.

• Korean Journal of Air-Conditioning and Refrigeration Engineering
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• v.13 no.2
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• pp.73-79
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• 2001

Experiment were carried out to investigate the heat transfer characteristics of an aluminum foam block as a porous heat sink on a heat source by a vertical air jet impingement that can be applied for electronics cooling. The performance of the aluminum foam heat sink was evaluated by the convective heat transfer coefficient on the heat source. At a fixed porosity, pore density ($\beta$) of the foam and Reynolds number Re were varied in the range of $\beta$a=10, 20, 40 PPI(Pore Per Inch) and $850\leqRe\leq25000$. A nozzle diameter and the nozzle-to-plate spacing were also varied. It was found that the convective heat transfer was enhanced by the aluminum foam heat sink with lower pore density due to relatively intensified flow through the foam block. The aluminum foam block with much reduced weight shows slightly better performance with larger Nusselt number, compared with the convectional heat sink.