• 한국가금학회지
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• 제38권1호
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• pp.1-4
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• 2011

A 35-day trial was conducted to examine the influence of floor density on the behaviour of broiler chickens. Day-old male broilers (n=756) were randomly assigned to one of four stocking densities (6 replicates of n=13, 25, 38 and 50) in 24 identical 2.6 $m^2$ pens. These stocking densities were coded very low (VL), low (L), medium (M) and high (H) and contained a floor space allowance per bird of 2,000 $cm^2$, 1,000 $cm^2$, 667 $cm^2$ and 500 $cm^2$, respectively. Scan sampling of all groups was carried out at 15-min intervals during two 1-h periods (10.00 h~11.00 h and 14.00 h~15.00 h) for five days each week. The numbers of birds engaged in different behavioural activities were recorded. It was found that the most common behaviour in all densities was lying. There was no clear effect of density during wks 1~4 of the trial, but in wk 5 birds in the L, M and H groups showed lower levels (P=0.07) of lying behaviour when compared to birds in the VL group suggesting that an increase in animal density results in decreased opportunities for undisturbed rest. This observation is supported by standing and walking behaviour, which was lower (P<0.05) in the VL group in wk 5. Foraging behaviour measured in the study by the numbers of birds pecking the ground declined as the trial progressed, but scratching increased in 2 wk then decreased. Birds in the VL group showed higher (P<0.05) level of pecking the ground behaviour compared to birds in the L, M and H groups, but scratching behaviour higher (P<0.05) and lower (P<0.05) in VL of 1 wk and 2 wk respectively. However, a peak in aggressive behaviour was observed in wk 2 and birds in the VL group showed less (P<0.05) agonistic behaviour than birds in the H and M groups. Other behaviours (dustbathing, preening, eating or drinking) were not influenced (P>0.05) by stocking density.

• Toxicological Research
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• 제34권3호
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• pp.221-229
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• 2018

Tenofovir nanoparticles are novel therapeutic intervention in human immunodeficiency virus (HIV) infection reaching the virus in their sanctuary sites. However, there has been no systemic toxicity testing of this formulation despite global concerns on the safety of nano drugs. Therefore, this study was designed to investigate the toxicity of Tenofovir nanoparticle (NTDF) on the liver and kidney using an animal model. Fifteen adult male Sprague-Dawley (SD) rats maintained at the animal house of the biomedical resources unit of the University of KwaZulu-Natal were weighed and divided into three groups. Control animals (A) were administered with normal saline (NS). The therapeutic doses of Tenofovir (TDF) and nanoparticles of Tenofovir (NTDF) were administered to group B and C and observed for signs of stress for four weeks after which animals were weighed and sacrificed. Liver and kidney were removed and fixed in formal saline, processed and stained using H/E, PAS and MT stains for light microscopy. Serum was obtained for renal function test (RFT) and liver function test (LFT). Cellular measurements and capturing were done using ImageJ and Leica software 2.0. Data were analysed using graph pad 6, p values < 0.05 were significant. We observed no signs of behavioural toxicity and no mortality during this study, however, in the kidneys, we reported mild morphological perturbations widening of Bowman's space, and vacuolations in glomerulus and tubules of TDF and NTDF animals. Also, there was a significant elevation of glycogen deposition in NTDF and TDF animals when compared with control. In the liver, there were mild histological changes with widening of sinusoidal spaces, vacuolations in hepatocytes and elevation of glycogen deposition in TDF and NTDF administered animals. In addition to this, there were no significant differences in stereological measurements and cell count, LFT, RFT, weight changes and organo-somatic index between treatment groups and control. In conclusion, NTDF and TDF in therapeutic doses can lead to mild hepatic and renal histological damage. Further studies are needed to understand the precise genetic mechanism.

• 한국응용약물학회:학술대회논문집
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• 한국응용약물학회 1998년도 Proceedings of UNESCO-internetwork Cooperative Regional Seminar and Workshop on Bioassay Guided Isolation of Bioactive Substances from Natural Products and Microbial Products
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• pp.170-170
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• 1998

In order to discover new types of 5-hydroxytryptamine antagonists, we have devoted our attention to investigating naturally occurring compounds having anti-5HT activity in vitro. Recently, ${\gamma}$-mangostin [1,3,6,7-tetrahydroxy-2,8-bis(3-methyl-2-bytenyl)-9H-xanthen-9-one] from the fruit hull of Garcinia mangostana Linn has been shown to be a selective antagonist for 5-hydroxytryptamine$_{2A}$ receptors in smooth muscle and platelets. It is of interesting that y-mangostin which does not have a nitrogen atom, possesses marked 5-$HT_{2A}$ receptor blocking activity. The present study was undertaken to investigate the effects of ${\gamma}$-mangostin on central 5-HT receptors by using animal behavioural models. Intracerebronventricular injection of ${\gamma}$-mangostin (10-40n mol/mouse) inhibited 5-fluoro-${\alpha}$-methyltryptamin (5-FMT) (45 mg kg$^{-1}$, i.p.)-induced head-twitch response in mice in the presence or absence of citalopram (5-HT-uptake inhibitor). Neither the 5-FMT- nor the 8-hydroxy-2-( di-n-propylamino )tetralin (5-HT$_{1A}$-agonist)-induced 5-HT syndrome (head weaving and hindlimb abduction) was affected by ${\gamma}$-mangostin. The locomotor activity stimulated by 5-FMT through the activation of at-adrenoceptors did not alter in the presence of ${\gamma}$-mangostin. 5-HT-induced inositol phosphates accumulation in mouse brain slices was abolished by ketanserin. ${\gamma}$-Mangostin caused a concentration-dependent inhibition of the inositol phosphates accumulation and the binding of [$^3H$]-spiperone, a specific 5-$HT_{2A}$ receptor antagonist, to mouse brain membranes. Kinetic analysis of the [$^H3$]-spiperone binding revealed that ${\gamma}$-mangostin increased the $_{d}$ value without affecting the $B_{max}$ value, indicating the mode of the competitive nature of the inhibition by ${\gamma}$-mangostin. These results suggest that ${\gamma}$-mangostin inhibits 5-FMT-induced head-twitch response in mice by blocking 5-$HT_{2A}$ receptors not by blocking the release of 5-HT from the central neurone. ${\gamma}$-Mangostin is a promising 5-$HT_{2A}$ receptors antagonist in the central nervous system.m.

• 대한약침학회지
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• 제20권1호
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• pp.23-28
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• 2017

Objectives: Exercise with partially restricted blood flow is a low-load, low-intensity resistance training regimen which may have the potential to increase muscle strength in the obese, elderly and frail who are unable to do high-load training. Restricted blood flow exercise has also been shown to affect blood vessel function variably and can, therefore, contribute to blood vessel dysfunction. This pilot study tests the hypothesis that unilateral resistance training of the leg extensors with partially restricted blood flow increases muscle strength and decreases vascular autoregulation. Methods: The subjects were nine normotensive, overweight, young adult African-Americans with low cardiorespiratory fitness who underwent unilateral training of the quadriceps' femoris muscles with partially restricted blood flow at 30% of the 1-repetition maximum (1-RM) load for 3 weeks. The 1-RM load and post-occlusion blood flow to the lower leg (calf) were measured during reactive hyperemia. Results: The 1-RM load increased in the trained legs from $77{\pm}3$ to $84{\pm}4 kg$ (P < 0.05) in the absence of a significant effect on the 1-RM load in the contralateral untrained legs (P > 0.1). Post-occlusion blood flow decreased significantly in the trained legs from $19{\pm}2$ to $13{\pm}2mL{\cdot}min^{-1}{\cdot}dL^{-1}$ (P < 0.05) and marginally in the contralateral untrained legs from $18{\pm}2$ to $16{\pm}1mL{\cdot}min^{-1}{\cdot}dL^{-1}$ (P = 0.09). Changes in post-occlusion blood flow to the skin overlying the trained and the contralateral untrained muscles were not significant. Conclusion: These results demonstrate that restricted blood flow exercise, which results in significant gains in muscle strength, may produce decrements in endothelial dysfunction and vascular autoregulation. Future studies should determine whether pharmacopuncture plays a role in treatments for such blood vessel dysfunction.

• 한국임상수의학회지
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• 제26권3호
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• pp.246-258
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• 2009

This study was conducted for behavioral characteristic analysis of the Asiatic Black Bear in a limited space. Behaviors of eight Asiatic Black Bears were classified into 13 normal stances and locomotor activities, 15 normal maintenance behaviors, 9 locomotory compulsive behaviors, 2 non-locomotory compulsive behaviors through the 3 years of monitoring. The bears had originally been released into the Jiri National Park for Asiatic Black Bear Restoration Project and were withdrawn again because of several reasons such as habituation to humans, and apiary damage. Through the monitoring of 6 hours per day during 3 months, classified behaviors were analyzed based on sex, age, observing month, observing timing, captivity period, and captive form. The total rate of stereotypic behaviors was $26.51{\pm}13.38%$. Among these, RA(Rest_A) was rated high as $47.32{\pm}18.32%$. In addition, SP(Standard pace), HR(Head rear), EP(Extended pace) were most frequently observed behaviors. The time budget of TFS(Two feet stand), SA(Sniff_A) and SB(Sniff_B) on females and younger individuals were relatively higher than male and older individuals. So we confirmed that females and younger individuals had more wariness and curiosity. As the period of captivity took longer, the rate of stereotypic behaviors was higher and more stereotypic behaviors were observed in the afternoon. At night, behaviors related with resting like Rest-A, Rest-B, Lying down, Lying on abdomen, Sitting were more frequently observed. We concluded that the captive state could affect the behaviors of Asiatic Black Bear and long term research should be necessary.

• Child Health Nursing Research
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• 제7권1호
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• pp.35-50
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• 2001

The ability of neonates to perceive and react to pain, has been acknowledged recently. Recent researches have been shown that even short term pain can have lasting negative effects. We know that most of the anatomical pathway and neurotrans-mitter function necessary for pain perception are fully or nearly fully developed in the neonatal period. Many people are still reluctant to believe that pain felt by neonates may be as severe as that felt by older children or adults yet. The objective of the study is to assess and compare the analgesic effects of orally administered sucrose and pacifiers. And to determine the synergistic analgesic effects of sucrose and pacifiers. The tools for this study is Lawrence's NIPS (neonatal infant pain scale) with behavioural pain responses and index for pain physiological reponses as heart rate, respiratory rate and degree of SaO₂. The participants are 96 healthy infants of neonatal age 1-7days and randomised to receive heel prick before 2minutes to blood sampling and physiological test in Nursery of K Medical University Hospital on May-July, 2000. The experimental group assigned to one of three treatment groups : no treatment; a pacifier; placebo(2ml 25％ sucrose). Collected data were analyzed with the SAS program using X²-test, ANOVA and Duncan's multiple range test as post hoc. The results were as follows. 1) Pain behaviour responses: The pain score of placebo group(2ml 25％ sucrose) is lower than no treatment group and pacifier group significantly(P=.000). Placebo group is different from no treatment and pacifier group with Duncan's multiple range test. 2) Pain physiologic responses (1) heart rate: The heart rate of placebo group(2ml 25％ sucrose) is different from that of no treatment group and pacifier group significantly(P=.000). The heart rate change of placebo group is less than the other groups. (2) respiratory rate: The respiratory rate of placebo group(2ml 25％ sucrose) is different from that of no treatment group and pacifier group insignificantly (P=.2340). But, the respiratory rate of placebo group is lower than the other groups. (3) SaO₂: The SaO₂ score of placebo group(2ml 25％ sucrose) is different from that of no treatment group and pacifier group insignificantly (P=.3265). But, the change of SaO₂ score of placebo group is less than the other groups. In conclusion, the sucrose placebo showed pain relief effect in behavior responses and less physiological responses. Accordingly, the sucrose placebo should be applied nursing intervention for simple pain management as heel prick.

• 대한지리학회지
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• 제29권2호
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• pp.202-215
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• 1994

본 연구에서는 지리학 분야에 있어서의 관광자원에 대한 연구동향을 검토함으로써 관광자원 연구의 이론적 기초를 제시하고자 하였다. 관광자원에 대한 지리적 접근은 크게 3 가지 주제로 범주화 할 수 있다. 첫째, 관광자원의 유형화와 공간적 분포에 관한 연구로 내 용상 관광자원의 개념과 범위, 기준설정을 토대로 한 관광자원 자체의 유형화와 그 지역적 분프특성에 근거한 공간적 유형화로 구분되어 발전하였다. 둘째, 관광현상을 유발시키고 관 광목적지를 결정하는 배경으로서 관광자원에 대한 인지 및 선호, 해석에 대한 행태론적 연 구이다. 셋째, 최근에 활발한 연구를 보이고 있는 관광자원의 개발에 따른 경제적 편익분석 및 문화적, 교육적, 환경적, 심리적 영향과 효과에 대한 연구동향을 들 수 있다. 종함검토 결 과 관광자원에 대한 지리적 연구는 그 공간적, 내용적 범위가 세분화, 전문화되고 있으며, 연구주제와 방법면에서도 구체적인 자료와 계량적, 분석적 틀을 기초한 실증적인 연구로 발 전해 가고 있다.

• 대한지리학회지
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• 제29권2호
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• pp.183-200
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• 1994

조선후기 경기지역은 한강수운을 비롯한 'X'자형 간선도로망의 중심에 입지하여 재 화와 정보, 기술의 유입이 他道에 비해 빠른 곳일 뿐 아니라, 행정. 상업.위수기능이 집중 하여 도회의 발전가능성이 많은 곳이었다. 조선후기의 도회는 고급행정중심지를 제외하면 대개 3,000-5,000정도의 인구를 보유한 작은 규모로 도회거주민은 관리, 상인, 장인, 노비 등 非農民層이 주류를 이루었다. 경기도 내에 비농업적인 도시기능을 수행한 곳은 한성과 개성 을 비롯해 모두 52곳이다. 이들 중 인구 3,000명 이상의 도회로 성장한 곳은 29곳인데 대부 분 조선시대의 간선도로망인 'X'자형 도로망중 서북-동남축을 따라 분포하였다. 경기도의 도회를 중심지의 인구규모와 도회기능의 소유 정도에 따라 계층화하면 6개의 등급으로 나눌 수 있다. 각 등급별 도회상황을 살펴 본 결과 1등급에서 4등급까지는 도회화의 정도가 차이 가 남을 알 수 있지만 4등급에서 6등급까지는 전체전인 도회의 모습에 있어서 그다지 큰 차 이가 나타나지는 않음을 알 수 있다.

• Asian-Australasian Journal of Animal Sciences
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• 제31권12호
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• pp.1991-2000
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• 2018

Objective: Triplet-born lambs are less likely to survive to weaning than twin-born or single-born lambs. Appropriate ewe-lamb bonding behaviours and lamb vigour behaviours are necessary for survival of lambs. The aim of this experiment was to determine whether maternal nutrition during late pregnancy influenced behaviour of the ewe and her lambs soon after birth, and to determine whether mid-pregnancy body condition score (BCS) influenced any behavioural response. Methods: The experiments included ewes that were in BCS 2.0, 2.5, or 3.0 in mid-pregnancy and were fed either ad libitum or to pregnancy-maintenance requirements in late-pregnancy (day 115 until 136 in experiment one, and day 128 until 141 in experiment two). The time taken for lambs to stand, contact dam, suck from dam and follow dam was recorded three to 18 h after birth. The number of high- and low-pitched bleats emitted by the ewe and lambs was recorded, along with maternal behaviour score (MBS) of the ewe. Lambs in experiment two underwent a maternal-recognition test at 12 or 24 h. Results: There were significant effects of feeding treatment on bleating behaviour of ewes and lambs, but these were inconsistent among BCS groups and between experiments. Lamb vigour behaviours were not affected by feeding treatment. In experiment one, there was no effect of feeding treatment or BCS on MBS, but in experiment two, ewes in BCS3 in mid-pregnancy had greater MBS than ewes in BCS2 in mid-pregnancy (MBS 3.1/5 vs MBS 2.1/5; p<0.05). Conclusion: Given there were no repeatable effects on behaviour of ewes and lambs, ad libitum feeding rather than feeding for pregnancy-maintenance requirements cannot be used to improve behaviours soon after birth of triplet-bearing ewes in BCS 2-3 and their lambs in extensive pastoral conditions.

• 벤처창업연구
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• 제17권5호
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• pp.223-235
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• 2022

본 연구는 중소기업의 관리자의 리더십 유형과 조직동일시을 이해하는 것을 목적으로 한다. 본 연구의 목적은 중소기업의 관리자 리더십과 조직동일시가 직무만족에 미치는 영향력과 조직신뢰의 매개효과를 분석하였다. 분석 자료는 중소기업 직원을 대상으로 하였으며, 수집된 자료는 다중회귀분석을 이용하여 분석하였다. 그 결과는 다음과 같다. 리더십 유형 중 변혁적 리더십과 거래적 리더십은 직무만족도에 유의미한 정(+)의 영향을 미쳤으며, 조직동일시는 직무만족도에 유의미한 정(+)의 영향을 미쳤다. 중소기업의 조직신뢰에 대한 매개효과는 변혁적 리더십→조직신뢰→직무만족도, 거래적 리더십→조직신뢰→직무만족도, 조직동일시→조직신뢰→직무만족도와의 관계에서 유의미한 영향을 미치는 것으로 나타났다. 본 연구결과를 통해서 중소기업 관리자의 리더십과 조직의 역할의 중요성을 알 수 있었다. 또한 중소기업은 조직비전을 통해서 조직구성원들에게 조직에 대한 긍정적인 이미지를 심어주는 전략이 필요하다.