• Title/Summary/Keyword: beauveria bassiana

Search Result 102, Processing Time 0.032 seconds

Statistical Optimization of Culture Conditions for the Production of Aphicidal Metabolites of Beauveria bassiana Bb08 (Beauveria bassiana Bb08의 살충성 물질 생산을 위한 배양조건의 통계적 최적화)

  • Go, Eunsu;Lim, Younghoon;Jeong, Hyeongchul;Choi, Jaepil;Park, Inseo;Kim, Jeong Jun;Lee, Dong-Jin;Kim, Keun
    • Microbiology and Biotechnology Letters
    • /
    • v.41 no.4
    • /
    • pp.398-406
    • /
    • 2013
  • For the maximal production of aphicidal metabolites produced by the Beauveria bassiana Bb08, statistical methods such as the Box-Behnken experimental design and response surface methodology were used. The fungal culture filtrate was sprayed towards 3-star aphids and the mortality was examined. After the statistical analysis of the aphid mortality, the optimal culture conditions were found to be a culture temperature of $26.2^{\circ}C$, medium pH 5.9, flask shaking speed of 209.0 rpm, and culture time of 5.9 days. The expected mortality on days 4, 5, and 6 after spraying the filtrate on to the aphids were 76.8%, 84.9%, and 89.4%, respectively. All 4 factors of the culture conditions significantly affected the production of the aphicidal metabolites, and the order of significance was temperature, pH, culture time and shaking speed.

Asexual Stage and Fruit Formation of Cordyceps staphylinidaecola (유충노랑곰보동충하초(Cordyceps staphylindaecola)의 불완전세대와 자실체 형성)

  • Sung, Jae-Mo;Hong, Sung-Jun;Humber, R.A.;Spatafora, J.W.
    • The Korean Journal of Mycology
    • /
    • v.31 no.1
    • /
    • pp.1-7
    • /
    • 2003
  • One hundred fifty one specimens of Beauveria spp. from 19 different locations were collected from September 1 to August 31, 2002. Most of the isolates were identified as Beauveria. bassiana. Cordyceps staphylinidaecola collected from Mt. Obong in Chunchon City covered the host with mycelia which were produced 1 to 4 stromata along with asexual spores. The size of bright yellow ununiform stromata were about 45 mm and the head about $17mm{\times}4mm$. Perithecia completely immersed were $530{\sim}550{\times}290{\sim}300{\mu}m$ in size and mainly scattered on the head. Ascospore produced in asci in the size of $400{\sim}450{\times}4{\sim}5{\mu}m$ developed thread-like secondary spores, which were directly separated into secondary conidial spores. Conidia produced at apical portion of synnemata were $2.6{\sim}3.4{\times}1.2{\sim}1.9{\mu}m$ in size. High density of mycelium was observed at $25^{\circ}C$ ranged from pH 6.5 to 8.5 after 11 days of inoculation. It took 15 to 18 days after inoculation to fully grow on the medium mixed brown rice with pupa. Mycelium developed stromata on the medium 30 days after completion of mycelial growth, where perithecia were produced in 40 days.

Influence of Systemic Fungicide on the Hematology of Silkworm Bombyx mori L. Infected with Beauveria bassiana

  • Kumar, Virendra;Nataraju, B.;Thiagarajan, T.;Dandin, S.B.
    • International Journal of Industrial Entomology and Biomaterials
    • /
    • v.6 no.1
    • /
    • pp.11-14
    • /
    • 2003
  • Systemic fungicides viz., Bavistin and Beyleton are reported to have curative effect against the infection of Beauveria bassiana in silkworm Bombyx mori L. To understand the influence of the systemic fungicides on the disease suppression/development, hematological studies were carried out. There was an increase in the percent total hemocyte count, granulocyte and plasmatocyte in silkworm treated with the systemic fungicides. It possibly indicates the influence of systemic fungicide on the hemocyte mediated defense system leading to the higher resistance and suppression of disease development.

Mosquito Control Using Entomopathogenic Fungi (곤충병원성 곰팡이를 이용한 모기 방제)

  • Choi, Kwang Shik;Jung, Hee-Young
    • The Korean Journal of Mycology
    • /
    • v.43 no.2
    • /
    • pp.77-87
    • /
    • 2015
  • Insects are commonly infected by fungal diseases and are mostly susceptible to them. Increasing levels of insecticide resistance has recently become an issue for control programs; thus, research has focused on mosquito control using entomopathogenic fungi, including fungal pathogens such as Beauveria bassiana, Metarhizium anisopliae, and Lagenidium giganteum. Review discusses entomopathogenic fungi related to control programs for mosquito transmitted vector-borne diseases such as dengue, filariasis, malaria, and yellow fever, and how to use entomopathogenic fungi for mosquito control.

Assessment of Skin Irritation Caused by S-(-)-10,11-Dihydroxyfarnesoic Acid Methyl Ester, a Metabolite of Beauveria bassiana CS1029

  • Kim, Min-A;Lee, Sang-Han
    • Current Research on Agriculture and Life Sciences
    • /
    • v.32 no.3
    • /
    • pp.145-148
    • /
    • 2014
  • To determine whether S-(-)-10,11-dihydroxyfarnesic acid methyl ester (DHFAME) produced by Beauveria bassiana CS1029 potentially causes acute skin irritation as a cosmetic ingredient, a skin toxicity test was conducted as recommended for compliance with Korea Food and Drug Administration regulations. New Zealand White rabbits were treated with 100 mg/dose of DHFAME according to standard guidelines. No significant skin lesions or inflammation was observed in the DHFAME-treated group. Furthermore, DHFAME did not appear to cause skin irritation, as assessed by clinical observation of the rabbits. Thus, when taken together, the present results suggest that DHFAME is a promising potential cosmetic ingredient that does not irritate the skin.

Multivariate Analysis on Invertebrate Communities in Litter and Soils of Japanese Red Pine Forests treated by Beauveria bassiana (백강균(白殭菌)을 처리(處理)한 소나무림의 낙엽(落葉)과 토양(土壤)에 서식(棲息)하는 무척주동물(無脊柱動物) 군집(群集)에 대한 다변량분석(多變量分析))

  • Kwon, Tae-Sung;Park, Young-Seuk;Shin, Sang-Chul;Lee, Buom-Young
    • Journal of Korean Society of Forest Science
    • /
    • v.90 no.5
    • /
    • pp.593-599
    • /
    • 2001
  • We tested if the treatment of Beauveria bassiana would influence invertebrate communities in litter and soils by multivariate analysis. The PCA (principal components analysis) was used for the analysis. Using the distances between communities in the ordination space, we carried out statistical tests whether any factors would influence structures of the communities. We did not found any significant effects of the Beauveria treatment on invertebrate communities in both litter and soils.

  • PDF

Efficient Inoculation Method of Beauveria bassiana for Production of Bombycis corpus and Evaluation of Its Liver Protection Activity (백강잠(白彊蠶) 생산을 위한 Beauveria bassiana의 효율적인 접종법 및 백장잠의 간보호 활성 검정)

  • Jung, I-Yeon;Hong, In-Pyo;Kang, Pil-Don;Nam, Sung-Hee;Kim, Mi-Ja
    • Journal of Sericultural and Entomological Science
    • /
    • v.47 no.1
    • /
    • pp.5-11
    • /
    • 2005
  • When inoculating with B. bassiana 101A for the mass production of B. corpus, the infection ratio was high with regardless of the treating time with highly-humidity if the concentration of spore was 1.0${\times}$$10^8$ spores/m/, but that was low if the concentration was 1.0${\times}$$10^7$ spores/m/. In the study of the activities according to the coserving temperature or days of the B. bassiana spawn, the infection ratio of 90% was maintained for 12 days in the temperature of $4^{\circ}C$. However, the infection ratio was rapidly dropped to the below of 5% after conserved for 48 hours in the temperature of $25^{\circ}C$. Besides, the activities of the original isolate had no difference after conserved for 12 months in the temperature of $4^{\circ}C$, so that the infection ratio 90% could be mintatined. In the measure of liver-protecting activities of B. bassiana 101A, the recovering effect was 43.5% and 65.7% respectively in the poisonous treatment induced with galactosamine, compared with liver-protecting activities of Silymarin and DDB in the $H_2O$ fraction. In the poisonous treatment induced with $CCl_4$ the recovering effect was 100% and 69.3% respectively, compared with liver-protecting activities of Silymarin and DDB in the EtOAc fraction.