• Title/Summary/Keyword: beamline

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Beamline Automation of RIKEN Structural Genomics Beamlines

  • Ida, Koh;Yamamoto, Masaki;Kumasaka, Takashi;Ueno, Go;Kanda, Hiroyuki;Miyano, Masashi;Ishikawa, Tetsuya
    • Journal of Photoscience
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    • v.9 no.2
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    • pp.463-465
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    • 2002

  • RIKEN Structural Genomics Beamlines have been constructed for the crystallographic analysis in the structural genomics research at synchrotron radiation facility SPring-8. Synchrotron radiation accelerates the crystallographic analysis of protein structure. The target of the research and development is focused on the automatic beamline operation to maximize beamline efficiency. We are developing the sample management system, which is composed of the sample auto-changer and the database system, for high-throughput data collection. The sample management system and the beamline operating system make it possible to execute automatic data collection without any operators. The beamlines will be ready for user operation in autumn 2002. The concept of automatic beamline operation and the present status of RIKEN Structural Genomics Beamlines will be presented.

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Performance Test of 4Cl Beamline for Protein Solution Scattering at the PLS (용액상의 단백질 구조 분석을 위한 PLS 4Cl빔라인의 성능 테스트)

  • Yu Chung-Jong;Kim Jehan;Kim Kwang-Woo;Kim Ghyung-Hwa;Lee Heung-Soo;Ree Moonhor;Kim Kyung-Jin
    • Journal of the Korean Vacuum Society
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    • v.14 no.3
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    • pp.138-142
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    • 2005

  • We tested performance of the 4C1 beamline for analyzing structures of proteins in solution using small angle X-ray scattering (SAXS) at the Pohang Light Source(PLS). Structurally well-known proteins such as lysozyme and $Bcl-XL(\vartriangle TM/\vartriangle loop)$ were used for the study. Low resolution solution structures of lysozyme and $Bcl-XL(\vartriangle TM/\vartriangle loop)$ were obtained at a resolution of at least i.2 nm, and the structures were basically same as those calculated from the crystal structures of the proteins. We also used $Bcl-XL(\vartriangle TM/\vartriangle loop)$ with a long flexible loop attached [$Bcl-XL(\vartriangleTM))$] and obtained significantly different data from $Bcl-XL(\vartriangle TM/\vartriangle loop)$, although the electron density map of the loop is known to be invisible from the crystal structure of $Bcl-XL(\vartriangleTM))$. We confirm that SAXS experiment is a powerful tool for the structural study of proteins in solution and the 4Cl beamline at the PLS is well-equipped and suitable for the protein solution SAXS experiment.

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Development of Real-time Control System for White bBamline and Microprobe Beamline (백색광 및 X선 미세탐침 빔라인용 실시간 제어시스템 개발)

  • 윤종철;이진원;고인수
    • 제어로봇시스템학회:학술대회논문집
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    • 1997.10a
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    • pp.748-751
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    • 1997

  • The White Beamline of the Pohang Accelerator Laboratory(PAL) consists of main and second slits, a microprobe system, two ion chambers, a video-microscope, and a Si(Li) detector. These machine components must be controlled remotely through computer system to make user experiments precise and speedy. A real-time computer control system was developed to control and monitor these machine components. A VNIEbus computer with OS-9 real-time operating system was used for low-level data acquisition and control. VME I/O modules were used for step motor control and scaler control. The software has modular structure for maximum performance and easy maintenance. We developed database, I/O driver, and control software. We used PC/Window95 for data logging and operator interface. Visual C++ was used graphical user interface programming. RS232C was used for communication between VME and PC.

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