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Influence of Plant Growth Substances on Cytolysome-like Organelles in the Mesophyll Cells of Soybean (식물생장소가 대두 엽육세포의 Cytolysome-like Organelle에 미치는 영향)

  • 김우갑
    • Journal of Plant Biology
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    • v.17 no.4
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    • pp.163-170
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    • 1974
  • Leaf tissue of Glycine max Merr. was fixed in para-formaldehyde-glutarldehyde and postfixed in osmium tetroxide or postassium permanganate for electron microscopy. The origin of cytolysome-like organelles of mesophyll cell was studied and changes of fine structure of the organelles according to treating solutions such as gibberellin (GA), kinethin (KI), 2,4-dichlorophenoxy acetic acid(2, 4-D) or 2, 4-D+GA(1mg/l, respectively) were observed. The cylolysome-like organelles differentiate in endoplasmic reticulum and plasmalemma, and they drop into vacuoles being isolated from the formers. They seem to change into myelin-like structure and to be degenerated by autodigestion. Cytolysome-like organelles involved in cell walls and vacuoles showed activity of acid phosphatase. In the group of GA and KI treatment, cytolysome-like organelles were similar to that of the control group. But in the treatmental groups of 2,4-D and 2,4-D+GA, myelin-like structures increased in size and autodigestion of this organelles were similar to that of the control group. But in the treatmental groups of 2,4-D and 2,4-D+GA, myelin-like structures increased in size and autodigestion of this organelle seemed to be accelerated. In the treatmental group of 2,4-D+GA, myelin-like structures shown high electron density were observed in cytoplasm and vacuoles together.

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A DFT Study on Alkali and Alkaline Earth Metal Encapsulated Fullerene-Like BeO Cluster

  • Ravaei, Isa;Beheshtian, Javad
    • Journal of the Korean Chemical Society
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    • v.61 no.6
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    • pp.311-319
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    • 2017
  • By using Density Functional Theory (DFT), we have performed alkali metal and alkaline earth metal inside fullerene-like BeO cluster (FLBeOC) in terms of energetic, geometric, charge transfer, work function and electronic properties. It has been found that encapsulated processes of the alkali metal are exothermic and thermodynamically more favorable than alkaline earth metal encapsulation, so that interaction energy ($E_{int}$) of the alkali metal encapsulation FLBeOC is in the range of -0.02 to -1.15 eV at level of theory. It is found that, the electronic properties of the pristine fullerene-like BeO cluster are much more sensitive to the alkali metal encapsulation in comparison to alkaline earth metal encapsulation. The alkali and alkaline earth metal encapsulated fullerene-like BeO cluster systems exhibit good sensitivity, promising electronic properties which may be useful for a wide variety of next-generation nano-sensor device components. The encapsulation of alkali and alkali earth metal may increase the electron emission current from the FLBeOC surface by reducing of the work function.

Crystal Structure and Physical Property of Tetragonal-like Epitaxial Bismuth Ferrites Film

  • Nam, Joong-Hee;Biegalski, Michael;Christen, Hans M.;Kim, Byung-Ik
    • Proceedings of the Korean Magnestics Society Conference
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    • 2011.06a
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    • pp.7-8
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    • 2011
  • Basically, the lattice mismatch between film and substrate can make those BiFeO3(BFO) films distorted with strain structure. BFO phase can be stabilized on LaAlO3(LAO) represents the example of a multiferroic with giant axial ratio. Its crystal structure is not strictly tetragonal, but tetragonal with a slight monoclinic distortion and related to the rotation of the oxygen octahedra. In this study, we show that phases with a tetragonal-like epitaxial BFO films can indeed be ferroelectric and also can be stabilized via epitaxial growth onto LAO. Recent reports on epitaxial BFO films show that the crystal structure changes from nearly rhombohedral ("R-like") to nearly tetragonal("T-like") at strains exceeding approximately -4.5%, with the "T-like" structure being characterized by a highly enhanced c/a ratio. While both the "R-like" and the "T-like" phases are monoclinic, our detailed x-ray diffraction results reveal asymmetry change from MA and MC type, respectively. By applying additional strain or by modifying the unit cell volume of the film by substituting Ba for Bi, the monoclinic distortion in the "T-like" MC phase is reduced, i.e. the system approaches a true tetragonal symmetry. There are two different M-H loops for $Bi_{1-x}Ba_xFeO_{3-{\delta}}$(BBFO) and BFO films on SrTiO3(STO) & LAO substrates. Along with the ferroelectric characterization, these magnetic data indicate that the BFO phase stabilized on LAO represents the first example of a multiferroic with giant axial ratio. However, there is a significant difference between this phase and other predicted ferroelectrics with a giant axial ratio: its crystal structure is not strictly tetragonal, but tetragonal with a slight monoclinic distortion. Therefore, in going from bulk to highly-strained films, a phase sequence of rhombohedral(R)-to-monoclinic ["R-like" MA-to-monoclinic, "T-like" MC-to-tetragonal (T)] is observed. This sequence is otherwise seen only near morphotropic phase boundaries in lead-based solid-solution perovskites (i.e. near a compositionally induced phase instability), where it can be controlled by electric field, temperature, or composition. Our results show that this evolution can occur in a lead-free, stoichiometric material and can be induced by stress alone. Those major results are summarized as follows ; 1) Ba-doping increases the unit cell volume, 2) BBFO on LAO can be fully strained up to x=0.08 as a strain limit (Fig. 1), 3) P(E) & M(H) properties can be tuned by the variation of composition, strain, and film thickness.

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A New Approach to Obtain Correct and Simplified Equation Applied to Inner Space Assessment for Capsule-like Superstructures

  • Jo, Jihee;Lim, Jong Kuk
    • Bulletin of the Korean Chemical Society
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    • v.34 no.6
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    • pp.1835-1838
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    • 2013
  • Polypyrrole-Gold (PPy/Au) segment nanowires are prepared using anodized aluminum oxide (AAO) templates and assembled into a curved superstructure. Since the shape of the obtained superstructures can be designed to be capsule-like with inner space for containing materials, and their openings and closures can be controlled with external stimuli, these structures can be useful for a large variety of applications. Inner space of capsule-like superstructures is an important factor for their applications, and the volume of the inner space can be assessed using the generalized equation suggested by J. K. Lim (Bull. Korean Chem. Soc. 33, 2699 (2012)). In this paper, we introduce a new approach to obtain correct and simplified equation without redundant assumption which was used to induce the previous equations, and recalculate the volume of the inner space in the capsule-like superstructure using a new equation.

Finding Impossible Differentials for Rijndael-like and 3D-like Structures

  • Cui, Ting;Jin, Chen-Hui
    • KSII Transactions on Internet and Information Systems (TIIS)
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    • v.7 no.3
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    • pp.509-521
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    • 2013
  • Impossible Differential Cryptanalysis (IDC) uses impossible differentials to discard wrong subkeys for the first or the last several rounds of block ciphers. Thus, the security of a block cipher against IDC can be evaluated by impossible differentials. This paper studies impossible differentials for Rijndael-like and 3D-like ciphers, we introduce methods to find 4-round impossible differentials of Rijndael-like ciphers and 6-round impossible differentials of 3D-like ciphers. Using our methods, various new impossible differentials of Rijndael and 3D could be searched out.

31P NMR Spectroscopy Revealed Adenylate kinase-like Activity and Phosphotransferase-like Activity from F1-ATPase of Escherichia coli

  • Kim, Hyun-Won
    • Bulletin of the Korean Chemical Society
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    • v.32 no.1
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    • pp.183-185
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    • 2011
  • Adenylate kinase-like activity and phosphotransferase-like activity from $F_1$-ATPase of Escherichia coli was revealed by $^{31}P$ NMR spectroscopy. Incubation of F1-ATPase with ADP in the presence of $Mg^{2+}$ shows the appearance of $^{31}P$ resonances from AMP and Pi, suggesting generation of AMP and ATP by adenylate kinase-like activity and the subsequent hydrolysis to Pi. Incubation of $F_1$-ATPase with ADP in the presence of methanol shows additional peak from methyl phosphate, suggesting phosphotransferase-like activity of $F_1$-ATPase. Both adenylate kinase-like activity and phosphotransferase-like activity has not been reported from $F_1$-ATPase of Escherichia coli. $^{31}P$ NMR could be a valuable tool for the investigation of phosphorous related enzyme.

Assessment of PLLIF Measurement for Spray Mass Distribution of Like-Doublet Injector (Like-Doublet 인젝터의 분무 질량분포 측정을 위한 PLLIF기법의 신뢰성 평가)

  • Jung Kihoon;Koh Hyeonseok;Yoon Youngbin
    • Journal of the Korean Society of Visualization
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    • v.1 no.1
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    • pp.98-106
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    • 2003
  • A PLLIF (Planar Liquid Laser Induced Fluorescence) technique has been known to be a useful tool for the measurement of the spray patterns for various spray injectors because it can obtain two-dimensional images with high spatial resolutions without any intrusion on the spray field. In case of dense spray, however, the secondary emission as well as the extinction of an incident laser beam or a fluorescence signal can cause errors in quantifying a mass distribution. Unfortunately, a like-doublet injector which has a dense spray zone at the center may not be a good example or the application of the PLLIF technique. Therefore, we took PLLIF data for the like-doublet injector with a 12 bit color CCD camera by varying laser power, and then assessed their accuracy by comparing with the data obtained with a mechanical patternator and a PDPA (Phase Doppler Particle Analyzer). The experimental results showed that the gray level of fluorescence signal increases nonlinearly due to a secondary emission at the dense spray zone but this nonlinearity can be avoided by reducing the incident laser beam power. In addition, the mass flux distribution of the spray could be obtained by using the mass concentration data from PLLIF technique and the velocity profiles of liquid drops, and this distribution showed good agreement with that of mechanical pattemator. Therefore, it is possible that the PLLIF technique can be successfully applied to finding the mass distributions of like-doublet injectors.

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The Nature of Water in Tactic Poly (2-Hydroxyethyl Methacrylate) Hydrogels

  • Kim, Eui-Hwan;Jeon, Sang-Il;Yoon, Sung-Chul;Jhon, Mu-Shik
    • Bulletin of the Korean Chemical Society
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    • v.2 no.2
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    • pp.60-66
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    • 1981
  • The hypothesis that three classes of water exist in hydrogels, namely X water (free water-like), Z-water (bound water-like), and Y water (interfacial water-like), has been verified and generally accepted. To further check the validity of this hypothesis and to study the nature of X, Y, and Z water as conformation changes, several experiments have been done using Tactic Poly(2-hydroxyethyl methacrylate) (P-HEMA) gels. Thermal expansively data for tactic P-HEMA gel was obtained. In each case of isotactic and syndiotactic P-HEMA, the higher water content gels showed an extremely sharp volume change at $0^{\circ}C$, indicating the presence of normal free water-like. Lower water content gels showed no anomalous change in thermal expansion, indicating that the water is bound water-like. The medium water content gels exhibited intermediate behavior. These results were also confirmed by bulk gel conductivity measurments. The differential scanning calorimeter(DSC) experiment was simply introduced to further verify the bound water-like quantities which was obtained by the method of dilatometry and specific conductivity. Observing the amounts of X, Y, and Z water with the change of tacticity, the similar content of bound water-like may be due to the same primary structure of isotactic and syndiotactic polymer and the difference in free and interfacial water-like content may be due to the difference in secondary and tertiary structure of tactic polymer. Therefore, as the polymer conformation varies, the free and interfacial water-like content will be varied. In order to demonstrate these concepts, Russel et al.'s CPK space-filling molecular models of isotactic and syndiotactic P-HEMA was utilized.

Hormonal Regulation of Insulin-Like Growth Factor Binding Protein Secretion by a Bovine Mammary Epithelial Cell Line

  • Kim, W.Y.;Chow, J.C.;Hanigan, M.D.;Calvert, C.C.;Ha, J.K.;Baldwin, R.L.
    • Asian-Australasian Journal of Animal Sciences
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    • v.10 no.2
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    • pp.233-239
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    • 1997
  • A mammary epithelial cell line (MAC-T) established as a model for lactation was utilized to identify and characterize effects of various hormones upon insulin-like growth factor binding protein secretion. Ligand and immunoblot analyses of conditioned media indicated that insulin-like growth factor binding protein-2 was secreted by MAC-T cells. Insulin-like growth factor-I stimulated insulin-like growth factor binding protein-2 secretion in a dose-dependent manner, but prolactin and bovine somatotropin did not alter insulin-like growth factor binding protein-2 secretion. Insulin increased and cortisol decreased insulin-like growth factor binding protein-2 secretion. Effects of insulin-like growth factor-I on insulin-like growth factor binding protein-2 secretion support previous studies using primary cultures of bovine mammary cells and bovine fibroblasts. Effects of cortisol and insulin on insulin-like growth factor binding protein-2 secretion may be explained by changes in protein synthesis. In addition, supraphysiological doses of insulin can cross-react with the insulin-like growth factor-I receptor and stimulate insulin-like growth factor binding protein-2 secretion. MAC-T cells provide a model system to study mechanisms that regulate local insulin-like growth factor-I bioactivity.

VP-ellipsis, Stripping, and the Functions of the Delimiter -to in Korean

  • Kim, So-Jee;Cho, Sae-Youn
    • Language and Information
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    • v.20 no.1
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    • pp.93-110
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    • 2016
  • VP-ellipsis constructions in English can be schematized as S + [NP finite-AUX __ ] where the underlined part is understood to be a VP. Similarly, the pattern S + NP[-to] can be observed in Korean colloquial contexts. Though the English VP-ellipsis sentence pattern and the Korean pattern superficially seem to be similar, the Korean pattern exhibits peculiar properties: Syntactically, the NP of the pattern should have the delimiter -to. Semantically, it may convey ambiguous readings: VP-ellipsis-like and/or Stripping-like interpretation. To account for the pattern at issue, we propose a base-generated analysis driven by the delimiter -to within a construction grammar. We claim that the mother of the NP[-to] in this pattern is an S whose meaning is ambiguous between a VP-ellipsis-like and a Stripping-like reading. Consequently, the code of the VP-ellipsis in English is finite auxiliary verbs while that of the pattern S + NP[-to] in Korean is the delimiter -to.

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