• 대한세포병리학회지
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• 제10권1호
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• pp.67-71
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• 1999

We report a case of 53-year-old man with plasmacytold transitional cell carcinoma of the urinary bladder, which may be confused with plasmacytoma. The patient initially presented with gross hematuria and dysuria for two months. Cystoscopy and radiologic studios revealed multiple intraluminal protruding masses on the urinary bladder invading perivesical fat tissue. After urinary cytologic examination and cystoscopic biopsy, radical cystectomy and pelvic lymph node dissections were done. Urine cytology showed single cells and poorly cohesive cells with round eccentric nuclei, bi-or multi-nucleation, indistinct nucleoli, coarse chromatin, and abundant basophilic cytoplasm within relatively clear background. The cytologic findings of tumor cells were similar to the plasma cells seen in plasmacytoma. The tumor of the bladder was composed on discohesive, individual cancer cells with diffuse pattern that simulated lymphoma or plasmacytoma. Immunohistochemical and electron microscopic studies clearly established the epithelial nature of the neoplasm. Recognition of this plasmacytoid type of transitional cell carcinoma of the urinary bladder can avoid the misdiagnosis.

• Preventive Nutrition and Food Science
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• 제21권4호
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• pp.323-329
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• 2016

Achyranthes japonica Nakai (AJN) water extract has a variety of physiological properties, including anti-diabetic, anti-cancer, anti-inflammatory, anti-microbial, and anti-oxidative activities. In the present study, the inhibitory effects of AJN extract were investigated in high affinity immunoglobulin E receptor ($Fc{\varepsilon}RI$)-mediated KU812F cells activation. AJN extract showed suppressive effects on histamine release and intracellular calcium [$Ca^{2+}$]i elevation from anti$Fc{\varepsilon}RI$ antibody (CRA-1)-stimulated cells in a dose-dependent manner. Flow cytometric analysis showed that AJN extract treatment caused a dose-dependent decrease in the cell surface $Fc{\varepsilon}RI$ expression and the binding between the cell surface $Fc{\varepsilon}RI$ and the IgE antibody. Moreover, reverse transcription-polymerase chain reaction analysis showed that levels of the mRNA for the $Fc{\varepsilon}RI$ ${\alpha}$ chain was decreased by treatment with AJN extract. These results indicate that AJN extract may exert anti-allergic effects via the inhibition of calcium influx and histamine release, which occurs as a result from the downregulation of the binding of IgE antibody to cell surface $Fc{\varepsilon}RI$. This mechanism may occur through $Fc{\varepsilon}RI$ expression inhibition.

• 한국자원식물학회지
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• 제28권3호
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• pp.321-328
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• 2015

Alpiniae officinarum Rhizoma (the rhizome of Alpinia officinarum Hance, known as lesser galangal), a family of Zingiberaceae, has been used to reduce pain of infection and inflammatory diseases in Asian countries. The present study was focused to evaluate the inhibitory degranulation effect of Alpiniae officinarum Rhizoma extract in RBL-2H3 rat basophilic leukemia cells. Cell viability was measured by MTT assay. RBL-2H3 cells were stimulated by phorbol 12-myristate 13-acetate and calcium ionophore A23187. Mast cell degranulation was analyzed by measuring release of β-hexosaminidase in RBL-2H3 cell. Gene expression was measured by qRT-PCR and signaling molecules were detected by immunoblotting. The Alpiniae officinarum Rhizoma extract suppressed β-hexosaminidase release in dose-dependent manner and inhibited cycloxygenase-2 and tumor necrosis factor-α gene expression. Furthermore, it was found that Alpiniae officinarum Rhizoma extract reduced mitogen-activated protein kinases, especially phosphorylated p38, at 0.75 ㎎/㎖ of Alpiniae officinarum Rhizoma extract concentrations. These data show that Alpiniae officinarum Rhizoma extract has immunosuppressive effect in mast cell induced allergic inflammation.

• 한국어류학회지
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• 제28권1호
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• pp.19-27
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• 2016

은대구의 소화관 상대길이 비는 1.52 (n=12)이며, 소화관은 위의 후방부에 5~6개의 유문수를 가진다. 점막주름의 형태는 식도와 위에서는 미분지형이지만 장에서는 분지형이다. 횡단면에서 소화관은 조직학적으로 점막층, 점막하층, 근육층 및 장막으로 구분할 수 있다. 식도의 점막상피층은 단층이며, 원주섬모상피세포들과 점액세포들로 구성된다. 위 점막층의 위선은 관상선으로 주세포, 벽세포 및 뮤신분비세포들로 구성된다. 뮤신분비세포는 원주형으로 AB-PAS (pH 2.5) 반응에서 분홍색과 푸른색을 나타내는 분비과립을 가진다. 장의 점막상피층은 단층이며, 원주섬모상피세포들과 배상세포들로 구성된다. 점막하층은 소성결합조직층으로 주로 교원섬유들로 구성되며, 식도에서 잘 발달되어 있다. 소화관의 근육층은 종주근층과 환상근층으로 구분되며, 위에서 잘 발달되어 있다. 간은 다수의 소엽구조와 담관들로 이루어져 있으며, H-E 염색에서 간세포의 세포질은 호산성이며, 핵과 인은 호염기성을 보였다. 췌장조직은 소화관 주변의 지방조직에 산재하며, 다수의 외분비세포들로 구성된 포상선이었다. H-E 염색에서 외분비선세포의 세포질은 호염기성을 나타내며, 다수의 호산성 전효소 과립들을 함유한다.

• 대한수의학회지
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• 제35권2호
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• pp.317-326
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• 1995

The purpose of this study was to establish a method for in vitro culture of C parvum isolated in Korea by determination of suitable cell model to complete development of this parasite. The result obtained were summerized as follows: 1. To determine the most suitable cell line, six types of cell line were examined by microscopy. All cell lines were infected with C parvum and showed the highest infection score in HmLu cells. 2. The staining methods including DMSO-modified acid-fast(A-F) stain, hematoxylin-eosin(H & E) stain and immunofluorescence antibody(IFA) stain were applied to examine the infection of C parvum in cell culture. These staining methods were possible to examine the infection of C parvum in cell culture. The most sensitive one was IFA staining technique. 3. Developmental stages of C parvum in HmLu cell were observed. After the initial 8 hour incubation period, some trophozoites were observed. The meronts and gametes were appeared at 24-48 hour post inoculation(PI), and oocysts were observed firstly at 48-72 hour PI. 4. In H & E stain, the parasite appeared as basophilic within parasitophorous vacuole membrane(PVM) and lying in cytoplasm at near the nucleus of the host cells. It was able to distinguish the type I, type II meronts and gametes. 5. In DMSO-modified acid-fast stain, specific stained parasites were appeared firstly after 48 hour PI. The parasites were showed with different degrees of staining bright red color within PVM. 6. The endogenous stages of parasites in HmLu cell recovered at 48, 96, 120 and 144 hour after inoculation were reacted with rabbit immunized serum in immunofluorescence antibody and avidin-biotin complex peroxidase staining technique.

• 한국임상수의학회지
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• 제40권4호
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• pp.298-302
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• 2023

A 7-year-old neutered male, domestic shorthair cat presented anorexia and lethargy. The complete blood cell count revealed severe non-regenerative anemia, lymphocytic leukocytosis, neutropenia, and thrombocytopenia. On the peripheral blood smear examination, medium to large lymphoblastic cells with moderate amounts of basophilic cytoplasm were observed in up to 70% of peripheral leukocytes. Feline leukemia and immunodeficiency viruses were not detected using a commercial diagnostic kit. While splenomegaly and blunt margins of the caudoventral liver were observed in abdominal radiography, changes in the intra-abdominal lymph nodes were not remarkable. Ultimately, flow cytometric immunophenotyping from the peripheral blood revealed a negative for B-cell markers (CD21-/CD79a-) and T-cell markers (CD3-/CD4-/CD5-/CD8-). Based on the hematological examination and the immunophenotyping assay, the cat was diagnosed with non-B, non-T acute lymphoblastic leukemia. Here, we report a rare case of non-B, non-T acute lymphoblastic leukemia to raise awareness and provide information on clinical symptoms and laboratory test and immunophenotyping analysis results.

• 한국식품과학회지
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• 제43권2호
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• pp.220-223
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• 2011

KU812F 세포는 세포표면에 $Fc{\varepsilon}RI$을 발현하고 있어, IgE를 매개로 한 알러지 반응에 있어 중요한 역할을 담당하고 있는 것으로 알려져 있다. KU812F 세포를 이용하여, $Fc{\varepsilon}RI$ ${\alpha}$ chain 발현에 있어 구절초 에탄올 추출물의 저해효과를 조사하였다. 본 연구 결과, $Fc{\varepsilon}RI$ ${\alpha}$ chain의 세포표면 발현량 및 mRNA수준을 구절초 추출물이 감소시키는 것을 flow cytometry 및 RT-PCR로 각각 확인하였다. 또한, 구절초 추출물은 CRA-1 자극에 의한 히스타민 유리를 농도 의존적으로 억제시켰다. 이러한 결과는 구절초 추출물이 $Fc{\varepsilon}RI$ ${\alpha}$ chain 발현을 감소시킴으로써, 히스타민 유리가 억제되고, 이를 통해 항알러지 활성에 기여하는 것으로 생각된다.

• Preventive Nutrition and Food Science
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• 제22권1호
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• pp.9-15
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• 2017

Vaccinium angustifolium, commonly known as the lowbush blueberry, is a rich source of flavonoids, with which various human physiological activities have been associated. The present study focuses on the investigation of the effect of the methanolic extract of V. angustifolium root extract (VAE) on high affinity immunoglobulin E receptor ($Fc{\varepsilon}RI$) ${\alpha}$ chain antibody (CRA-1)-induced allergic reaction in human basophilic KU812F cells. The total phenolic content of VAE was found to be $170{\pm}1.9mg$ gallic acid equivalents/g. Flow cytometry analysis revealed that the cell surface expression of $Fc{\varepsilon}RI$ was suppressed in a concentration-dependent manner upon culture with VAE. Reverse-transcriptase polymerase chain reaction analysis showed that the mRNA level of the $Fc{\varepsilon}RI$ ${\alpha}$ chain was reduced in a concentration-dependent manner as a result of VAE treatment. Western blot analysis revealed that the protein expression of $Fc{\varepsilon}RI$ and the phosphorylation of extracellular signal-regulated kinases (ERK) 1/2 were concentration-dependently inhibited by VAE. We determined that VAE inhibited anti-CRA-1-induced histamine release, in addition to the elevation of intracellular calcium concentration ([$Ca^{2+}$]i), in a concentration-dependent manner. These results indicate that VAE may exert an anti-allergic effect via the inhibition of calcium influx and histamine release, which occurs as a result of the down-regulation of $Fc{\varepsilon}RI$ expression through inhibition of ERK 1/2 activation.

• Applied Microscopy
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• 제40권1호
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• pp.9-14
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• 2010

경골어류, 잉어과, 모래무지아과에 속하는 쉬리(Coreoleuciscus splendidus)의 난자형성과정을 광학현미경으로 관찰한 결과는 다음과 같다. 쉬리의 난소는 한 쌍으로 부레와 창자사이에 위치하고 있었으며 회색을 띠었다. 난소의 형태는 장타원형(장축 20 mm, 단축 5 mm)이었다. 난원세포는 다각형으로 세포질은 호염기성이었고 핵 안에 여러 개의 인들이 분포하고 있었다. 초기시기의 제1난모세포(primary oocyte)는 난원세포에 비하여 크기가 커졌으며 난황포(yolk vesicle)들은 핵 주변부에는 관찰되지 않았고 세포질의 가장자리에만 국한적으로 형성되기 시작하였다. 제1난모세포가 발달함에 따라서 난황포는 바깥 가장자리에서 안쪽으로 증식되어 핵 쪽으로 이동되었으며 난막 바깥쪽은 짙은 푸른색의 여포상피(follicular epithelium)로 싸여 있었다. 그러나 핵의 주변부는 아직 푸른색을 띠고 있었다. 제2난모세포는 세포질이 전체적으로 호산성이었으며 난황포가 세포질 전체에 분포하였다. 성숙한 난자는 난자의 크기가 상당히 커졌으며 난막이 두꺼워졌다. 또한 난황포는 난황괴(yolk mass)로 전환되었다. 이상과 같이 쉬리의 난자형성과정은 난세포의 크기 증가, 난황낭의 축적, 세포질 내 염기성 물질이 산성으로 전환 및 난막의 발달로 요약될 수 있으며 일반적인 다른 과의 담수경골어류와 큰 차이는 없는 것으로 확인되었다. 본 어종의 종특이성을 찾기 위해서 난막의 미세구조적 연구가 추가적으로 필요할 것으로 사료된다.

• 생명과학회지
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• 제8권5호
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• pp.582-588
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• 1998

징거미 새우의 정소는 한 쌍의 관으로 되어 있고, 정소의 중앙 후반부에서 기원된 수정관은 제 5보각의 기부에 위치한 생식공까지 연결되어 있다. 수정관은 기부, 나선부, 말단부, 사정관으로 구분되었다. 수정관은 simple columnar ephithelium, high columnar ephithelium, 종주근과 환상근으로 이루어져 있다. AG는 수정관의 말단부에서 사정관에 걸쳐 위치하고 있다. AG의 형태는 불규칙한 세포 덩어리로 되어져 있고, hematoxyline에 약하게 염색되는 염색질과 둥글고 커다란 핵을 가진다. 핵질과 세포질은 염기성 핵막을 경계로 쉽게 구분되나, 세포질과 세포질사이의 경계는 그 구별이 어렵다. 암컷의 복판에 부착된 정포는 호산성기질, 호염기성기질 그리고 호염기성기질에 싸여있는 한 쌍의 정자덩어리로 구성되어 있다. 성숙정자는 압정형태이고, 두부는 가운데 부분이 약간 오목한 원반형이고, 미부는 두부 중앙부 하단의 약간 볼록한 부분에서 연장된 하나의 spike를 이루고 있다. 정자의 크기는 두부의 직경이 약 8 $\mu$m, 미부의 길이가 약 10 $\mu$m였다.