Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
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Inhibitory Effects of Chrysanthemum zawadsaki Ethanolic Extract on $Fc{\varepsilon}RI$ ${\alpha}$ Chain Expression

구절초 에탄올 추출물의 $Fc{\varepsilon}RI$ ${\alpha}$ chain 발현 저해효과

  • Shim, Sun-Yup (Institute of Marine Life Science, Pukyong National University) ;
  • Byun, Dae-Seok (Department of Food Science and Nutrition, Pukyong National University)
  • 심선엽 (부경대학교 해양생명과학연구소) ;
  • 변대석 (부경대학교 식품영양학과)
  • Received : 2011.01.10
  • Accepted : 2011.02.22
  • Published : 2011.04.30
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Abstract

Human basophilic KU812F cells express a high affinity for IgE receptor, $Fc{\varepsilon}RI$, which plays an important role in immunoglobulin E (IgE)-mediated allergic reactions. Using the human basophilic KU812F cells, we assessed the inhibitory effects of the ethanolic extract from Chrysanthemum zawadsaki(CZ) on $Fc{\varepsilon}RI$ ${\alpha}$ chain expression. Flow cytometric analysis showed that cell surface $Fc{\varepsilon}RI$ ${\alpha}$ chain expression was suppressed when the cells were cultured with CZ extract. RT-PCR analysis showed that the mRNAs for $Fc{\varepsilon}RI$ ${\alpha}$ chain decreased by the treatment of CZ extract. Moreover, CZ extract was also observed to result in a reduction in the release of histamine from anti-$Fc{\varepsilon}RI$ antibody (CRA-1)-stimulated cells. These results suggest that CZ extract may exert anti-allergic activity through down-regulation of $Fc{\varepsilon}RI$ ${\alpha}$ chain expression and a subsequent decrease in histamine release.

KU812F 세포는 세포표면에 $Fc{\varepsilon}RI$을 발현하고 있어, IgE를 매개로 한 알러지 반응에 있어 중요한 역할을 담당하고 있는 것으로 알려져 있다. KU812F 세포를 이용하여, $Fc{\varepsilon}RI$ ${\alpha}$ chain 발현에 있어 구절초 에탄올 추출물의 저해효과를 조사하였다. 본 연구 결과, $Fc{\varepsilon}RI$ ${\alpha}$ chain의 세포표면 발현량 및 mRNA수준을 구절초 추출물이 감소시키는 것을 flow cytometry 및 RT-PCR로 각각 확인하였다. 또한, 구절초 추출물은 CRA-1 자극에 의한 히스타민 유리를 농도 의존적으로 억제시켰다. 이러한 결과는 구절초 추출물이 $Fc{\varepsilon}RI$ ${\alpha}$ chain 발현을 감소시킴으로써, 히스타민 유리가 억제되고, 이를 통해 항알러지 활성에 기여하는 것으로 생각된다.

Keywords

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