• Journal of Forest and Environmental Science
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• 제37권2호
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• pp.163-167
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• 2021

Mushroom survey and collection were conducted in the Dong Hua Sao National Biodiversity Conservation Area (DHSNBCA), which is located about 30 km east of Pakse and about 10 km south of the town of Paksong, Champasak Province of Lao-PDR, from from at the early July to October in 2019. During this periods, mushroom specimens were collected from 6 different locations, and then they were identified and classified into 103 species, 74 genera, 37 families, 15 orders and 6 classes by morphological and molecular analyses. The mushrooms belongs to Ascomycota were classified into 10 species, 6 genera, 4 families, 3 orders and 2 classes, while those belongs to Basidiomycota were classified into 92 species, 67 genera, 32 families, 11 orders, and 3 classes, respectively. In addition, 1 species of Myxomycota was also identified. Among these mushrooms, the most species-rich families are Marasmiaceae (17.3%), Polyporaceae (13.1%), Mycenaceae (8.9%), Agricaceae (8.3%), Xylariaceae (6.5%), Auriculariaceae (4.8%), Ganodermtaceae (3.6%), Dacrymycetaceae (3.0%), Meruliaceae (3.0%), Russulaceae (3.0%) and comprised 71.4% of the total specimens identified.

• Journal of Species Research
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• 제10권4호
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• pp.336-343
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• 2021

In 2020, as a subset study to discover indigenous yeast species in Korea, a total of 22 yeast species were isolated from soil samples collected in Gwangju-si, Gyeonggi-do. Among them, 10 strains were unreported species. From the high 26S rRNA gene sequence similarity and formation of a robust phylogenetic clade with the closest species, it was determined that each strain belonged independent and predefined yeast species. The 22 strains were assigned to the genera Dothiora (1 strain), Sarocladium (1 strain), Tetrapisispora (1 strain) and Torulaspora (2 strains) of the phylum Ascomycota; the genera Erythrobasidium (1 strain), Leucosporidium (4 strains), Ustilentyloma (2 strains), Fellozyma (1 strain), Sampaiozyma (2 strains), Filobasidium (1 strain), Solicoccozyma (2 strains) and Vishniacozyma (4 strains) of the phylum Basidiomycota. This is the first official report of the following species in Korea: Dothiora cannabinae (1 strain), Sarocladium strictum (1 strain), Fellozyma inositophila (1 strain), Filobasidium magnum (1 strain), Solicoccozyma phenolicus (1 strain), Solicoccozyma terreus(1 strain), Vishniacozyma tephrensis(1 strain) and Vishniacozyma victoriae (3 strains). Cell morphology, phenotypic features and biochemical features are described in the Species Description section.

• Journal of Species Research
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• 제10권4호
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• pp.350-355
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• 2021

In 2020, 11 Basidiomycetous yeast strains were isolated from soil samples collected from the forests of Namhansanseong in Korea. Among them, seven species were reported, but four species were unreported in Korea. To identify wild yeasts, pairwise sequence comparisons of D1/D2 domain of the 26S rRNA were performed using Basic Local Alignment Search Tool (BLAST). The cell morphologies and assimilation test are observed by phase contrast microscope and API 20C AUX kit, respectively. The 11 strains were assigned to the genera Rhodotorula (4 strains) of the order Sporidiobolales of the class Microbotryomycetes; and Cryptococcus(2 strains), Goffeauzyma (1 strains), Naganishia (2 strains) of the order Filobasidiales and Saitozyma (2 strains) of the order Tremellales of the class Tremellomycetes in the phylum Basidiomycota. The unreported yeast strains Cryptococcus gastricus 20n5-2, Goffeauzyma gilvescens 20n2-7, Naganishia adeliensis 20n8-1, and Naganishia friedmannii 20n24-1 belong to the family Filobasidiaceae. All strains had oval shaped cells and cream-colored colonies cultured on on YM agar for 3 days. In this study, we focus on the description of four unreported yeast species in Korea.

• Journal of Species Research
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• 제12권3호
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• pp.197-202
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• 2023

The purpose of this study was to isolate and identify wild yeasts from the soil collected in Gwangju and Pocheon City, Gyeonggi Province, Republic of Korea. Among 10 strains, six strains were already reported, but four strains were unrecorded in Republic of Korea. To identify wild yeast strains, pairwise sequence comparisons of the D1/D2 region of the 26S rRNA gene sequence were performed using Basic Local Alignment Search Tool (BLAST). The cell morphologies were observed by phase contrast microscope and assimilation tests were carried out using API 20C AUX kit. The 10 strains were assigned to the phyla Basidiomycota (8 strains) and Ascomycota (2strains). The unrecorded four yeast strains, NH33, NH19, NH20, and YP416, belong to the phylum Basidiomycota and the genera Buckleyzyma, Leucosporidium, Holtermanniales, and Mrakia, respectively. All strains had oval-shaped and polar budding cells. In this research, the morphological and biochemical properties of four unreported yeast species were characterized intensively, which were not officially reported in Korea.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2017년도 9th Asian Crop Science Association conference
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• pp.364-364
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• 2017

Saemangeum reclaimed land is a part of Saemangeum Development Project. Most of the persistent problems of Saemangeum reclaimed land remain to be related to soil salinity. Soil salinity is a major abiotic factor related to microbial community structure and also fungi have been reported to be more sensitive to salinity stress than bacteria. The aim of this study was conducted to investigate the effect of soil salinity levels on the microbial communities in Saemangeum reclaimed land using 454 pyrosequencing analysis. Soil samples was collected from 12 sites of in Saemangeum reclaimed land. For pyrosequencing, 27F/518R (bacteria) and ITS3/ITS4 (fungi) primers were used containing the Roche 454 pyrosequencing adaptor-key-linker (underlined) and unique barcodes (X). Pyrosequencing was performed by Chun's Lab (Seoul, Korea) using the standard shotgun sequencing reagents and a 454 GS FLX Titanium sequencing System (Roche, Inc.). In the soil samples, Proteobacteria (bacteria) and Ascomycota (fungi) shows the highest relative abundance in all the soil sample sites. Proteobacteria, Bacteroidetes, Plantomycetes, Gemmatimonadetes and Parcubacteria were shown to have significantly higher abundance in high salinity level soils than low salinity level soils, while Acidobacteria and Nitrospirae has significantly higher relative abundance in low salinity level soils. The abundance of fungal, Ascomycota has the highest relative abundance in soil samples, followed by Basidiomycota, Chlorophyta, Zygomycota and Chytridiomycota. Basidiomycota, Zygomycota, Glomeromycota and Cerozoa were show significantly higher relative abundance in low salinity level soils. The principal coordinate analysis (PCoA) and correlation analysis shown to salinity-related soil parameters such as ECe, Na+, SAR and EPS were affected to bacterial and fungal community structure. Proteobacteria, Bacteroidetes, Plantomycetes exhibited significantly positive correlation with soil salinity, while Acidobacteria exhibited significantly negative correlation. In the case of fungal community, Basidiomycota and Zygomycota were seen show significantly negative correlation with salinity related soil parameters. These results suggest that provide understanding effect of soil salinity on microbial community structure and correlation of microbial community with soil parameters in Saemangeum reclaimed land.

• 한국식품과학회지
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• 제37권1호
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• pp.67-72
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• 2005

수삼 및 수삼을 기질로 한 배양물로부터 조사포닌을 분리하여 TLC를 확인한 결과 목질진흙버섯 및 영지버섯 균사체 배양물 조사포닌에서 $Rg_{2}$와 $Rh_{1}$ 위치에서 spot을 볼 수 있었으며, 노루궁뎅이버섯 균사체 배양물에서는 $Rg_{3}$와 $Rh_{1}$과 같은 위치에서 spot을 볼 수 있었다. 배양물의 총페놀성 화합물 함량은 수삼, 목질진흙버섯, 영지버섯 및 노루궁뎅이버섯 균사체 배양물 조사포닌에서 1.04, 1.11, 1.45, 1.29%로 나타나 수삼보다는 수삼 배양물에서 높았으며, 특히 영지버섯 균사체 배양물에서 가장 높은 폐놀성 화합물 함량을 나타내었다. 수삼 배양물 조사포닌의 전자공여능의 경우 목질진흙버섯 균사체 배양물 1mg/mL 및 10mg/mL에서 각각 92.8, 94.9%로 가장 높았다. 지질과산화 억제효과는 영지버섯 균사체 배양물 조사포닌이 29.5%로 목질진흙버섯 균사체나 노루궁뎅이버섯 균사체 배양물 보다 높았다. Tyrosinase 저해활성은 영지버섯 균사체 배양물 조사포닌이 65.5%로 가장 높았다.

• Mycobiology
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• 제37권4호
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• pp.305-307
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• 2009

An unrecorded Ceriporiopsis species was collected at Mt. Gariwang, Gangwon Province, in 2008. Based on morphological characteristics, such as a fully resupinate basidiocarp, a reddish white to pinkish poroid hymenophore and a monomitic hyphal system with clamp connections, the species was identified as Ceriporiopsis resinascens. This is the first report of Ceriporiopsis resinascens in Korea. We confirmed the identity of the species as Ceriporiopsis resinascens based on ITS sequence analysis.

• Mycobiology
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• 제48권2호
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• pp.95-103
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• 2020

The genus Termitomyces (Lyophyllaceae, Basidiomycota) is often associated with fungus-feeding termites (Macrotermitinae) due to their strong symbiotic relationships. The genus is widely found exclusively in certain regions of Africa and Asia. They are recognized as edible mushroom within Southeast Asia as well. But it is often misidentified based on morphology by the local communities especially in Malaysia for Chlorophyllum molybdites which is a highly poisonous mushroom. Thus, it is necessary to study the genus for Malaysia with the synergy of using both morphological and molecular identification. In this study, we aim to describe another new species as an addition to the genus Termitomyces found within Sabah, Malaysia. We generated two new sequences (nrLSU and mtSSU) for the new species and a total of 28 nrLSU and mtSSU sequences were retrieved from GenBank for the phylogenetic analysis using maximum likelihood and Bayesian inferences. We identified that the new collection from Sabah province is a new species and named as Termitomyces gilvus based on the termites found in the mound. A phylogeny tree made from the concatenated genes of LSU and mtSSU suggests that T. gilvus is closely related to T. bulborhizus from China. According to our results, the combination of molecular and morphology proved to be a robust approach to re-evaluate the taxonomic status of Termitomyces species in Malaysia. Additional surveys are needed to verify the species diversity and clarify their geographic distribution.

• Mycobiology
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• 제48권4호
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• pp.288-295
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• 2020

Species of Laccaria (Hydnangiaceae, Agaricales, and Basidiomycota) are well-known ectomycorrhizal symbionts of a broad range of hosts. Laccaria species are characterized by brown, orange, or purple colored basidiocarps, and globose or oblong, echinulate and multinucleate basidiospores. While some Laccaria species are easily identified at the species level using only the morphological characteristics, others are hard to distinguish at the species level due to small differences in morphology. Heretofore, ten Laccaria species have been reported in Korea. While studying the fungal diversity in the National Parks of Korea, two new Laccaria species were discovered. Species identification was done based on molecular analyses (ITS, 28S rDNA, rpb2, and tef1), then were confirmed by their corresponding morphologies. The two newly discovered Laccaria species are proposed here as Laccaria macrobasidia and Laccaria griseolilacina. The unique morphological characters of L. macrobasidia that distinguish it from its closely related species are orange-brown colored basidiocarp, long basidia and the absence of cheilocystidia. L. griseolilacina is characterized by a light grayish lavender-colored pileus and the absence of cheilocystidia. Two new species are described and illustrated in the present paper.

• 실내환경 및 냄새 학회지
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• 제16권1호
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• pp.54-63
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• 2017

The Automobile HVAC system is a habitat for odor-associated fungal communities. We investigated the odor-associated fungal community in an automobile HVAC system using a high-throughput DNA sequencing method. The fungal community structure was evaluated via metagenome analysis. At the phylum level, Ascomycota and Basidiomycota were detected, accounting for 43.41% and 56.49% of the fungal community in the HVAC system, respectively. Columnosphaeria (8.31%), Didymella (5.60%), Davidiella (5.50%), Microxyphium (4.24%), unclassified Pleosporales (2.90%), and Cladosporium (2.79%) were abundant at phylum of Ascomycota and Christiansenia (36.72%), Rhodotorula (10.48%), and Sporidiobolus (2.34%) were abundant at phylum of Basidiomycota. A total of 22 genera of fungi were isolated and identified from the evaporators of the HVAC systems which support fungal growth and biofilm formation. Among them, Cladosporium, Penicillium, Aspergillus, and Alternaria are the most representative odor-associated fungi in HVAC systems. They were reported to form biofilm on the surface of HVAC systems with other bacteria by hypha. In addition, they produce various mVOCs such as 3-methyl-1-butanol, acetic acid, butanoic acid, and methyl isobutyl ketone. Our findings may be useful for extending the understanding of odor-associated fungal communities in automobile HVAC systems.