• Animal cells and systems
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• v.16 no.1
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• pp.1-10
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• 2012

Most living organisms synchronize their physiological and behavioral activities with the daily changes in the environment using intrinsic time-keeping systems called circadian clocks. In mammals, the key molecular features of the internal clock are transcription- and translational-based negative feedback loops, in which clock-specific transcription factors activate the periodic expression of their own repressors, thereby generating the circadian rhythms. CLOCK and BMAL1, the basic helix-loop-helix (bHLH)/PAS transcription factors, constitute the positive limb of the molecular clock oscillator. Recent investigations have shown that various levels of posttranslational regulation work in concert with CLOCK/BMAL1 in mediating circadian and cellular stimuli to control and reset the circadian rhythmicity. Here we review how the CLOCK and BMAL1 activities are regulated by intracellular distribution, posttranslational modification, and the recruitment of various epigenetic regulators in response to circadian and cellular signaling pathways.

• Journal of Integrative Natural Science
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• v.5 no.3
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• pp.198-210
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• 2012

Circadian rhythms govern a remarkable variety of metabolic and physiological functions. Accumulating epidemiological and genetic evidence indicates that the disruption of circadian rhythms might be directly linked to cancer. Intriguingly, several molecular gears constituting the clock machinery have been found to establish functional interplays with regulators of the cell cycle, and alterations in clock function could lead to aberrant cellular proliferation. In addition, connections between the circadian clock and cellular metabolism have been identified that are regulated by chromatin remodelling. This suggests that abnormal metabolism in cancer could also be a consequence of a disrupted circadian clock. Therefore, a comprehensive understanding of the molecular links that connect the circadian clock to the cell cycle and metabolism could provide therapeutic benefit against certain human neoplasias.

• BMB Reports
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• v.49 no.11
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• pp.587-589
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• 2016

The circadian clock system enables organisms to anticipate the rhythmic environmental changes and to manifest behavior and physiology at advantageous times of the day. Transcriptional/translational feedback loop (TTFL) is the basic feature of the eukaryotic circadian clock and is based on the rhythmic association of circadian transcriptional activator and repressor. In Drosophila, repression of dCLOCK/CYCLE (dCLK/CYC) mediated transcription by PERIOD (PER) is critical for inducing circadian rhythms of gene expression. Pacemaker neurons in the brain control specific circadian behaviors upon environmental timing cues such as light and temperature cycle. We show that amino acids 657-707 of dCLK are important for the transcriptional activation and the association with PER both in vitro and in vivo. Flies expressing dCLK lacking AA657-707 in $Clk^{out}$ genetic background, homologous to the mouse Clock allele where exon 19 region is deleted, display pacemaker-neuron-dependent perturbation of the molecular clockwork. The molecular rhythms in light-cycle-sensitive pacemaker neurons such as ventral lateral neurons ($LN_vs$) were significantly disrupted, but those in temperature-cycle-sensitive pacemaker neurons such as dorsal neurons (DNs) were robust. Our results suggest that the dCLK-controlled TTFL diversify in a pacemaker-neuron-dependent manner which may contribute to specific functions such as different sensitivities to entraining cues.

• Journal of Integrative Natural Science
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• v.16 no.3
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• pp.75-80
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• 2023

Bipolar disorder is a mental illness characterized by extreme mood and behavioral swings, such as highs of euphoria and lows of depression. It is a socially significant disorder in which people with the disorder experience intense mood swings and, for those with severe bipolar disorder, it is even difficult leading a normal life. High stress levels in people with mental illness can lead to neuroendocrine disruption, and it is strongly linked to aging. When the neuroendocrine system becomes vulnerable to these mental illnesses and stress, it is likely to accelerate aging. And it's the epigenetic clock that can measure the extent of this accelerated aging. The Epi clock, a pan tissue clock, measures aging through DNA methylation, and the degree of methylation is modified and changed by environmental conditions in the body. Therefore we wanted to check the changes in the epigenetic age of the patients with bipolar disorder. While we found no significant differences in epigenetic age, we did confirm the possibility that people with bipolar disorder have different methylation than normal people. We also found that the EPIC array data fit better on the Epi clock than on the Horvath clock with age-accelerated data from normal people.

• Publications of The Korean Astronomical Society
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• v.22 no.4
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• pp.189-199
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• 2007

In this paper, we describe the proposed KVN (Korean VLBI Network) clock system in order to make the observation of the VLBI effectively. In general, the GPS system is widely used for the time information in the single dish observation. In the case of VLBI observation, a very high precise frequency standard is needed to perform the observation in accordance with the observation frequency using the radio telescope with over 100km distance. The objective of the high precise clock system is to insert the time-tagging information to the observed data and to synchronize it with the same clock in overall equipments which used in station. The AHM (Active Hydrogen Maser) and clock system are basically used as a frequency standard equipments at VLBI station. This system is also adopted in KVN. The proposed KVN clock system at each station consists of the AHM, GPS time comparator, standard clock system, time distributor, and frequency standard distributor. The basic experiments were performed to check the AHM system specification and to verify the effectiveness of implemented KVN clock system. In this paper, we briefly introduce the KVN clock system configuration and experimental results.

• Journal of Satellite, Information and Communications
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• v.10 no.2
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• pp.102-108
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• 2015

In general, two way satellite communication systems based on TDMA(Time Division Multiple Access) require network clock synchronization between hub station and remote terminals. This paper describes basic concepts for network clock synchronization based on NCR(Network Clock Reference) clock recovery scheme as suggested in DVB-S2/ RCS2 international standards. in addition, a new NCR insertion method has been proposed and evaluated in terms of supporting CCM mode as well as ACM mode which optimizes throughput by changing code rates and modulation types ranging from QPSK to 32-APSK.

• Proceedings of the Korean Institute of Navigation and Port Research Conference
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• v.1
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• pp.309-314
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• 2006

In general DGPS system, the correction message is transferred to users by wireless modem. To cover wide area, many DGPS station should be needed. And DGPS users must have a wireless modem that is not necessary in standalone GPS. But SBAS users don't need a wireless modem to receive DGPS corrections because SBAS correction message is transmitted from the GEO satellite by L1 frequency band. SBAS signal is generated in the GUS(Geo Uplink Subsystem) and uplink to the GEO satellite. This uplink transmission process causes two problems that are not existed in GPS. The one is a time delay in the uplink signal. The other is an ionospheric problem on uplink signal, code delay and carrier phase advance. These two problems cause ranging error to user. Another critical ranging error factor is clock synchronization. SBAS reference clock must be synchronized with GPS clock for an accurate ranging service. The time delay can be removed by close loop control. We propose uplink ionospheric error correcting algorithm for C/A code and carrier. As a result, the ranging accuracy increased high. To synchronize SBAS reference clock with GPS clock, I reviewed synchronization algorithm. And I modified it because the algorithm didn't consider doppler that caused by satellites' dynamics. SBAS reference clock synchronized with GPS clock in high accuracy by modified algorithm. We think that this paper will contribute to basic research for constructing satellite based DGPS system.

• Journal of Astronomy and Space Sciences
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• v.28 no.1
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• pp.79-91
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• 2011

Ongnu (Jade Clepsydra; also called Heumgyeonggaknu) is a water clock was made by Jang Yeong-sil in 1438. It is not only an automatic water clock that makes the sound at every hour on the hour by striking bell, drum and gong, but also an astronomical clock that shows the sun's movement over time. Ongnu's power mechanism used is a water-hammering method applied to automatic time-signal device. The appearance of Ongnu is modeled by Gasan (pasted-paper imitation mountain) and Binpungdo (landscape of farming work scene) is drawn at the foot of the mountain. The structure of Ongnu is divided into the top of the mountain, the foot of the mountain and the flatland. There located are sun-movement device, Ongnyeo (jade female immortal; I) and Four gods (shaped of animal-like immortals) at the top of the mountain, Sasin (jack hour) and Musa (warrior) at the foot of the mountain, and Twelve gods, Ongnyeo (II) and Gwanin on the flatland. In this study, we clearly and systematically understood the time-announcing mechanism of each puppet. Also, we showed the working mechanism of the sun-movement device. Finally, we completely established the 3D model of Ongnu based on this study.

• Journal of Engineering Education Research
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• v.13 no.2
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• pp.28-32
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• 2010

The mechanical clock was designed and produced by students as a trial theme of PBL class. The limit of making time is three weeks and main material is limited to paper. Only a basic mechanism drawing is given. As the principle of mechanical clock is not complex and does not need the special knowledge of mechanical engineering, even lower grade student seems to understand enough. This subject can train the skill of knowledge composition, creativity and the problem-solving ability. After execution,the validity and performance as the theme of PBL class was evaluated. As a result, the following findings were obtained. The paper made mechanical clock could be produced in three weeks. And, by additional improvements of the product, the enough strength and stable movement were achieved. Students can learn that many kinds of knowledge through actual experiences of design and making were required in manufacturing the real products. The proposed PBL style class is suitable as an introduction and training subject for lower grade student in department of engineering.

• Journal of Integrative Natural Science
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• v.16 no.2
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• pp.61-67
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• 2023

Human body ages differently due to environmental, genetic and pathological factors. DNA methylation patterns also differs depending on various factors such as aging and several other diseases. The aging clock model, which uses these differences to predict age, analyzes DNA methylation patterns, recognizes age-specific patterns, predicts age, and grasps the speed and degree of aging. Aging occurs in everyone and causes various problems such as deterioration of physical ability and complications. Alzheimer's disease is a disease associated with aging and the most common brain degenerative disease. This disease causes various cognitive functions disabilities such as dementia and impaired judgment to motor functions, making daily life impossible. It has been reported that the incidence and progression of this disease increase with aging, and that increased phosphorylation of Aβ and tau proteins, which are overexpressed in this disease and accelerates epigenetic aging. It has also been reported that DNA methylation is significantly increased in the hippocampus and entorhinal cortex of Alzheimer's disease patients. Therefore, we calculated the biological age using the Epi clock, a pan-tissue aging clock model, and confirmed that the epigenetic age of patients suffering from Alzheimer's disease is lower than their actual age. Also, it was confirmed to slow down aging.