• Earthquakes and Structures
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• v.9 no.1
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• pp.29-48
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• 2015

The main purpose of this work is to compare different criteria for the seismic strengthening of RC framed buildings in order to find the optimal combinations of these retrofitting techniques. To this end, a numerical investigation is carried out with reference to the town hall of Spilinga (Italy), an RC framed structure with an L-shaped plan built at the beginning of the 1960s. Five structures are considered, derived from the first by incorporating: carbon fibre reinforced polymer (FRP)-wrapping of all columns; base-isolation, with high-damping-laminated-rubber bearings (HDLRBs); added damping, with hysteretic damped braces (HYDBs); FRP-wrapping of the first storey columns combined with base-isolation or added damping. A three-dimensional fibre model of the primary and retrofitted structures is considered; bilinear and trilinear laws idealize, respectively, the behaviour of the HYDB, providing that the buckling be prevented, and the FRP-wrapping, without resistance in compression, while the response of the HDLRB is simulated by using a viscoelastic linear model. The effectiveness of the retrofitting solutions is tested with nonlinear dynamic analyses based on biaxial accelerograms, whose response spectra match those in the Italian seismic code.

• Earthquakes and Structures
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• v.19 no.4
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• pp.261-272
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• 2020

Recent severe earthquakes in and around the vital public places worldwide indicate the severe vulnerability of ground excitation to be assailed. Reducing the effect of seismic lateral load in structural design is an important conception. Essentially, seismic isolation is required to shield the superstructure in such a way that the building superstructure would not move when the ground is shaking. This study explores the effectiveness, design, and practical feasibility of base isolation systems to reduce seismic demands on buildings of varying elevations. Thus, static and dynamic analyses were conducted based on site-specific bi-directional earthquakes for base-isolated as well as fixed-based buildings. Remarkably, it was discovered that isolators used in low-rise to high-rise structures tend to significantly decrease the structural responses of seismic prone buildings. The higher allowable horizontal displacement induces structural flexibility and ensure good structural health of the building stories. Reinforcement from vertical and horizontal members can be reduced in significant amounts for BI buildings. Thus, although incorporating base isolators increases the initial outlay, it considerably diminishes the total structural cost.

• Journal of the Korean Chemical Society
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• v.63 no.1
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• pp.29-36
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• 2019

A potentially tetradentate Schiff base ligand, 2-((2-((pyridin-2-ylmethylene)amino)ethyl)amino)ethan-1-ol (PMAE), and its cadmium(II) complex, [$Cd(PMAE)I_2$] (1), were prepared and characterized by elemental analysis, FT-IR, Raman, $^1H$ and $^{13}C$ NMR spectroscopies and single-crystal X-ray diffraction. In the crystal structure of 1, the cadmium atom has a slightly distorted square-pyramidal geometry and a $CdN_3I_2$ environment in which the PMAE acts as an $N_3$-donor. In the crystal packing of the complex, the alcohol and amine groups of the coordinated ligands participate in hydrogen bonding with iodide ions and form $R^2{_2}(14)$ and $R^2{_2}(8)$ hydrogen bond motifs, respectively. In addition to the hydrogen bonds, the crystal network is stabilized by ${\pi}-{\pi}$ stacking interactions between pyridine rings. The thermodynamic stability of the isolated ligand and its cadmium complex along with their charge distribution patterns were studied by DFT and NBO analysis.

• Nuclear Engineering and Technology
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• v.55 no.4
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• pp.1269-1279
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• 2023

Pool boiling experiments are performed within an isolated bubble regime at inclination angles of 0° and 45°. When a bubble grows and departs from the heating surface, the pressure, buoyancy, and surface tension force play important roles. The curvature and base diameter are required to calculate the pressure force, the bubble volume is required to calculate the buoyancy force, and the contact angle and base diameter are required to calculate the surface tension force. The contact angle, base diameter, and volume of the bubbles are evaluated using images captured via a high-speed camera. The surface tension force equation proposed by Fritz is modified with the contact angles obtained in this study. When the bubble grows, the contact angle decreases slowly. However, when the bubble departs, the contact angle rapidly increases owing to necking. At an inclination angle of 0°, the contact angle is calculated as 82.88° at departure. Additionally, the advancing and receding contact angles are calculated as 70.25° and 82.28° at departure, respectively, at an inclination angle of 45°. The dynamic behaviors of bubble growth and departure are discussed with forces by pressure, buoyancy, and surface tension.

• The Plant Pathology Journal
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• v.34 no.1
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• pp.59-64
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• 2018

Bacteriophages of Acidovorax citrulli, the causal agent of bacterial fruit blotch, were isolated from 39 watermelon, pumpkin, and cucumber leaf samples collected from various regions of Korea and tested against 18 A. citrulli strains. Among the six phages isolated, ACP17 forms the largest plaque, and exhibits the morphology of phages in the Myoviridae family with a head diameter of $100{\pm}5nm$ and tail length of $150{\pm}5nm$. ACP17 has eclipse and latent periods of $25{\pm}5min$ and $50{\pm}5min$, respectively, and a burst size of 120. The genome of ACP17 is 156,281 base pairs with a G + C content of 58.7%, 263 open reading frames, and 4 transfer RNA genes. Blast search and phylogenetic analysis of the major capsid protein showed that ACP17 has limited homology to two Stentrophomonas phages, suggesting that ACP17 is a new type of Myoviridae isolated from A. citrulli.

• Fisheries and Aquatic Sciences
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• v.12 no.4
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• pp.265-275
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• 2009

G Protein-coupled receptors (GPCRs) mediating wide ranges of physiological responses is one of the most attractive targets for drug development. Rhodopsin, a dim-light photoreceptor, has been extensively used as a model system for structural and functional study of GPCRs. Fish have rhodopsin finely-tuned to their habitats where the intensity and the wavelength of lights are changed depending on its water-depth. To study the detailed molecular characteristics of GPCR architecture and to understand the fishery light-sensing system, genes encoding rod opsins were isolated from fishes living under different photic environments. Full-length rod opsin genes were obtained by combination of PCR amplification and DNA walking strategy of genomic DNA isolated from olive flounder, P. olivaceus, Japanese eel, A. japonica, and Common carp C. carpio. Deduced amino acid sequences showed a typical feature of rod opsins including the sites for Schiffs base formation (Lys296) and its counter ion (Glu113), disulfide formation (Cys110 and Cys187), and palmitoylation (Cys322 and Cys323) although Cys322 is replaced by Phe in Japanese eel. Comparison of opsins by amino acid sequence alignment indicated the closest similarity between P. olivaceus and H. hippoglossus (94%), A. japonica and A. anguilla (98%), and C. carpio and C. auratus (95%), respectively.

• Korean Journal of Pharmacognosy
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• v.27 no.2
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• pp.91-95
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• 1996

In the course of searching for acetylcholinesterase inhibitors from crude drugs, it was found that total MeOH extract of Corydalis Tuber showed significant inhibitory effect on acetylcholinesterase. To isolate acetylcholinesterase inhibitors from Corydalis Tuber, total MeOH extract of the the crude drug was subjected to activity guided fractionation. The MeOH extract was suspended in water and fractionated with methylene chloride and subjected to acid-base fractionation. Silica gel column chromatography of the basic fraction which showed significant inhibitory effect on acetylcholinesterase was carried out and 5 subfractions (1-5) were obtained. From subtraction 4, compound I was isolated. The structure of isolated compound I was identified by spectroscopic parameters of $^1H-NMR$, $^{13}C-NMR$, EI-MS and FAB-MS. The compound I was identified as berberine. It was found from the Lineweaver-Burk plot that berberine was a reversible and specific inhibitor of acetylcholinesterase having 90% inhibitory effect at the concentration of $2.5{\mu}M$.

• Korean Journal of Veterinary Service
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• v.26 no.2
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• pp.105-111
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• 2003

From February 2000 to December 2001, A total of 1,785 samples was taken from beef and pork carcasses in Seoul. Seven(0.69%) Listeria spp. were isolated from the 1,014 of beef carcasses, and five(0.65%) were isolated from the 771 of pork carcasses. The isolates were identified L monocytogenes by API listeria, and VIDAS LMO kit, serological test and PCR assay were preformed. A total 12 strains of L monocytogenes were isolated form samples tested and all of the strains were classified into serotype 1. PCR primers are selected to amplify a 520-base pair(bp) DNA fragment from the listeolysin O gene(hlyA) of Listeria monocytogenes. A 520-bp product was detected in PCR with DNA from L monocytogenes, but not from the other Listeria species tested.

• Earthquakes and Structures
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• v.23 no.6
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• pp.517-526
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• 2022

Previous studies have shown that pile-soil interactions have significant influences on the isolation efficiency of an isolated structure. However, most of the existing tests were carried out using a 1-g shaking table, which cannot reproduce the soil stresses resulting in distortion of the simulated pile-soil interactions. In this study, a centrifuge shaking table modelling of the seismic responses of a friction pendulum bearing isolated structure with a pile foundation under earthquakes were conducted. The pile foundation structure was designed and constructed with a scale factor of 1:100. Two layers of the foundation soil, i.e., the bottom layer was made of plaster and the upper layer was normal soil, were carefully prepared to meet the similitude requirement. Seismic responses, including strains, displacement, acceleration, and soil pressure were collected. The settlement of the soil, sliding of the isolator, dynamic amplification factor and bending moment of the piles were analysed to reveal the influence of the soil structure interaction on the seismic performance of the structure. It is found that the soil rotates significantly under earthquake motions and the peak rotation is about 0.021 degree under 24.0 g motions. The isolator cannot return to the initial position after the tests because of the unrecoverable deformation of the soil and the friction between the curved surface of the slider and the concave plate.

• Asian-Australasian Journal of Animal Sciences
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• v.22 no.11
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• pp.1555-1565
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• 2009

Anaerobic rumen fungi have been regarded as good genetic resources for enzyme production which might be useful for feed supplements, bio-energy production, bio-remediation and other industrial purposes. In this study, an expressed sequence tag (EST) library of the rumen anaerobic fungus Neocallimastix frontalis was constructed and functional genes from the EST library were analyzed to elucidate carbohydrate metabolism of anaerobic fungi. From 10,080 acquired clones, 9,569 clones with average size of 628 bp were selected for analysis. After the assembling process, 1,410 contigs were assembled and 1,369 sequences remained as singletons. 1,192 sequences were matched with proteins in the public data base with known function and 693 of them were matched with proteins isolated from fungi. One hundred and fifty four sequences were classified as genes related with biological process and 328 sequences were classified as genes related with cellular components. Most of the enzymes in the pathway of glucose metabolism were successfully isolated via construction of 10,080 ESTs. Four kinds of hemi-cellulase were isolated such as mannanase, xylose isomerase, xylan esterase, and xylanase. Five $\beta$-glucosidases with at least three different conserved domain structures were isolated. Ten cellulases with at least five different conserved domain structures were isolated. This is the first solid data supporting the expression of a multiple enzyme system in the fungus N. frontalis for polysaccharide hydrolysis.