• Journal of Life Science
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• v.9 no.6
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• pp.653-658
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• 1999

A novel marine microorganism, Vibrio sp. YE-101, was isolated from pufferfish and investigated for its ability to synthesize tetrodotoxin (TTX). Various strains isolated from the intestine of pufferfish were grown on TCBS agar plate, and then cultured on Ocean Research Institute (ORI) medium supplemented with 3% NaCl at 23$^{\circ}C$ for 3days. The cells were harvested, disrupted, fractionated by Bio-Gel P-2 column chromatography and then TTX-producing strain, Vibrio sp. YE-101, was identified using mouse bioassay. The isolated TTX from Vibrio sp. YE-101 was also analyzed and identified by HPLC and gas chromatography-mass spectrometer (GC-MS). The mass fragmentation of trimethylsilyl derivatives of C9-base of TTX from Vibrio sp. YE-101 was interpreted and the pattern of fragmentation was same with that of authentic standard. The purfied TTX was also positive to the mouse bioassay, which clearly represents that Vibrio sp. YE-101 can synthesize TTX.

• Proceedings of the Earthquake Engineering Society of Korea Conference
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• 2005.03a
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• pp.487-494
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• 2005

In this study, a set of artificial earthquake time histories, which can be used for the earthquake-resistant design of seismically isolated highway bridges, was presented. In addition, adequateness of the generated ground accelerations was investigated. These were performed based on the seismic design standard for seismically isolated bridges. Total of 22 acceleration time histories were generated for each soil condition by the spectral method. The time histories were verified to meet the code provisions including (1) mean response spectrum at control frequencies, (2) EPGA (effective peak ground acceleration), and (3) correlation coefficient. Finally, the maximum response corresponding to four time histories and the mean response associated with seven time histories were computed using the generated acceleration time histories, which shows validity of the proposed artificial earthquake time histories.

• Earthquakes and Structures
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• v.4 no.3
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• pp.285-297
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• 2013

The effect of soil structure interaction (SSI) on seismic response of a multi-degree-of-freedom structure isolated with a friction pendulum system (FPS) is studied. In the analysis, the soil is considered as an elastic continuum and is modeled using the finite element method. The effect of SSI on response of the structure is evaluated for twenty far-field and twenty near-fault earthquake ground motions. The effect of friction coefficient of sliding material of FPS on SSI is also studied. The results of the study show that the seismic response of the structure increases for majority of the earthquake ground motions due to SSI. The sliding displacement and base shear are underestimated if SSI effects are ignored in the seismic analysis of structures isolated with FPS.

• Microbiology and Biotechnology Letters
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• v.22 no.6
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• pp.571-576
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• 1994

One strain of cellulose-producing Acetobacter was isolated from the traditionally fermen- ted grape vinegar in Korea. The isolated strain, designated as KI strain was identified as the Acetobacter xylinum with respect to physiological and biochemical characteristics. KI produced acetic acid from ethanol, and then decomposed acetate to CO$_{2}$ and H$_{2}$O. When the isolated strain was cultivated statically in broth culture, a thick cellulose pellicle was formed. KI was tolerance of 8% ethanol and 30% glucose, and the isolate was positive in ketogenesis from glycerol, $\gamma$-pyrone from glucose and fructose, and 2-ketogluconic acid from glucose. KI strain possessed straight-chain C$_{18:1}$, C$_{16:0}$, and C$_{14:0}$ fatty acid, and contained ubiquinone Q$_{9}$ and Q$_{10}$ as isoprenoid quinone. DNA base composition of KI strain was 57.6% G+C.

• Proceedings of the Earthquake Engineering Society of Korea Conference
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• 1999.10a
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• pp.212-219
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• 1999

In this paper we investigated an applicability of earthquake regulations for seismic-isolated building structures which has been used currently and propose an efficient method for vertical distribution of seismic loads. The distribution of force is revised in UBC-94 as vertical distribution of force of UBC(Uniform Building Code)-91 is not sufficient safety but its distribution is inefficient expensive because of similar expression to fixed-based structures. In order to overcome this difficulties improved vertical distribution to fixed-based structures. In order to overcome this difficulties improved vertical distribution of seismic load is proposed using two degrees-of-freedom isolated structures and mode shape of fixed-based structures. Efficiency and accuracy of the proposed method are verified through analysis of an example structures with moment resisting frame and shear walls so this study approximate to dynamic analysis results in each case.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.14 no.3
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• pp.463-472
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• 1994

A method of stochastic response analysis of base-isolated fluid-filled pool structures subject to random ground excitations is studied. Fluid-structure interaction effects between the flexible walls and contained fluid are taken into account in the form of added mass matrix derived by FEM modeling of the contained fluid motion. The stationary ground excitation is represented by Modified Clough-Penzien spectral model and the nonstationary one is obtained by imposing an envelope function on the stationary one. The stationary and nonstationary response statistics of the two different isolation systems are obtained by solving the governing Lyapunov covariance matrix differential equations.

• Journal of the Computational Structural Engineering Institute of Korea
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• v.20 no.2
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• pp.147-155
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• 2007

In this study, the performance of the modified sliding mode control proposed in the previous study is evaluated for seismic response control of nonlinear hysteretic structures. Modified sliding mode control(MSMC) utilizes the target derivative of Lyapunov function in order to calculate control force, and its performance was evaluated only lot linear structures in the previous study. However, considering that most structures subject to strong earthquake show nonlinear hysteretic behivior, the results from the previous study have limitations in practical application. The results from numerical analyses of single degree of freedom systems and base isolated system, which were described using Bouc-Wen model, indicate that the proposed MSMC algorithm shows better control performance than the existing sliding mode controller.

• Earthquakes and Structures
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• v.10 no.6
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• pp.1451-1465
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• 2016

The effectiveness of base isolation (BI) systems for mitigation of seismic vibration of bridges have been extensively studied in the past. It is well established in those studies that the performance of BI system is largely dependent on the characteristics of isolator yield strength. For optimum design of such systems, normally a standard nonlinear optimization problem is formulated to minimize the maximum response of the structure, referred as Stochastic Structural Optimization (SSO). The SSO of BI system is usually performed with reference to a problem of unconstrained optimization without imposing any restriction on the maximum isolator displacement. In this regard it is important to note that the isolator displacement should not be arbitrarily large to fulfil the serviceability requirements and to avoid the possibility of pounding to the adjacent units. The present study is intended to incorporate the effect of excessive isolator displacement in optimizing BI system to control seismic vibration effect of bridges. In doing so, the necessary stochastic response of the isolated bridge needs to be optimized is obtained in the framework of statistical linearization of the related nonlinear random vibration problem. A simply supported bridge is taken up to elucidate the effect of constraint condition on optimum design and overall performance of the isolated bridge compared to that of obtained by the conventional unconstrained optimization approach.

• Smart Structures and Systems
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• v.15 no.3
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• pp.847-862
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• 2015

Typical base isolated buildings are designed so that the superstructure remains elastic in design-level earthquakes, though the isolation layer is often quite nonlinear using, e.g., hysteretic elements such as lead-rubber bearings and friction pendulum bearings. Similarly, other well-performing structural control systems keep the structure within the linear range except during the most extreme of excitations. Design optimization of these isolators or other structural control systems requires computationally-expensive response simulations of the (mostly or fully) linear structural system with the nonlinear structural control devices. Standard nonlinear structural analysis algorithms ignore the localized nature of these nonlinearities when computing responses. This paper proposes an approach for the computationally-efficient optimal design of passive isolators by extending a methodology previously developed by the authors for accelerating the response calculation of mostly linear systems with local features (linear or nonlinear, deterministic or random). The methodology is explained and applied to a numerical example of a base isolated building with a hysteretic isolation layer. The computational efficiency of the proposed approach is shown to be significant for this simple problem, and is expected to be even more dramatic for more complex systems.

• Earthquakes and Structures
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• v.9 no.3
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• pp.603-623
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• 2015

The scale and nature of the recent earthquakes in the world and the related earthquake disaster index coerce the concerned community to become anxious about it. Therefore, it is crucial that seismic lateral load effect will be appropriately considered in structural design. Application of seismic isolation system stands as a consistent alternative against this hazard. The objective of the study is to evaluate the structural and economic feasibility of reinforced concrete (RC) buildings with base isolation located in medium risk seismic region. Linear and nonlinear dynamic analyses as well as linear static analysis under site-specific bi-directional seismic excitation have been carried out for both fixed based (FB) and base isolated (BI) buildings in the present study. The superstructure and base of buildings are modeled in a 3D finite element model by consistent mass approach having six degrees of freedom at each node. The floor slabs are simulated as rigid diaphragms. Lead rubber bearing (LRB) and High damping rubber bearing (HDRB) are used as isolation device. Change of structural behaviors and savings in construction costing are evaluated. The study shows that for low to medium rise buildings, isolators can reduce muscular amount of base shears, base moments and floor accelerations for building at soft to medium stiff soil. Allowable higher horizontal displacement induces structural flexibility. Though incorporating isolator increases the outlay, overall structural cost may be reduced. The application of base isolation system confirms a potential to be used as a viable solution in economic building design.