• Applied Microscopy
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• 제27권4호
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• pp.373-389
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• 1997

Morphological difference of the renal glomerulus at different age groups have been studies in one week-old, five weeks-old, eight weeks-old, six months-old, twelve months-old, eighteen months-old, twenty-four months-old, and thirty months-old ICR mice. Pieces of the tissue taken from the renal corticies were fixed in 2.5% glutaraldehyde-1.5% paraformaldehyde solution (0.1 Millonig's phosphate buffer pH 7.3), and 1% osmium tetroxide solution (0.1 M Millonig's phosphate buffer, pH 7.3), and were embedded in araldite mixture. The ultrathin sections were stained with uranyl acetate and lead citrate solution, and were observed under a JEM 100CX-II electron microscope. The results were as follows: 1 In the one week-old mouse, thicknesses of the three layers of the glomeluar basal lamina (lamina densa, lamina rara interna and lamina rara externa) are similar, but in the five weeks-old mouse, thick lamina densa becomes a greater portion of the thickness of whole glomerular basal lamina. 2. No difference was noticed between thickness of the renal glomerular basal lamina of the five weeks-old mouse compare with that of the one week-old one, but basal lamina of the eight weeks-old one is thickened considerably and thicknesses were maintained through twelve months-old one. After eighteen months, the thickness of the glomerular basal lamina is increased remarkably. 3. After eighteen months, electron dense deposits within the basal lamina of the renal glomeruli are observed frequently. 4. Amount of the microfilaments in the mesangial cells and the mesangial matrices are increasing during aging. 5. The thicknesses of the basal laminae of the Bowman's capsule are increasing during aging. 6. After twenty four months, the proximal tubular cell-like parietal cells with well developed microvilli are observed frequently. From the above results, it was suggested that the renal glomerulus matures structurally in five weeks, and the function of the glomerulus is suppressed after eighteen months.

• Applied Microscopy
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• 제17권1호
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• pp.47-64
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• 1987

Morphological difference of the renal glomerulus at different age groups have been studied in young (three month-old), adult (twelve month-old) and old (thirty month-old) Fisher strain 344 rats. Pieces of the tissues were taken from renal corticies prefixed with 2.5% glutaraldehyde-1.5% paraformaldehyde (0.1 M Millonig's phosphate buffer, pH 7.3), following by post-fixation with 1% osmium tetroxide (0.1 M Millonig's phosphate buffer, pH 7.3) and embedded within Araldite. The ultrathin sections contrasted with uranyl acetate and lead citrate were observed under a JEM 100CX electron microscope. The mean thickness of glomerular basal lamina and Bowman's capsule were determined by measuring the thinnest portion of basal lamina, and by taking the average of 50 readings from electron micrographs at different ages. The numerical changes of the slit pores were compared based upon the numbers over the length of 10um of glomerular basal lamina. The results were as follow: 1. The thickness of glomerular basal lamina is increased during aging; 140.4 nm in young rats, 270.0 nm in adult ones, and 437.8 nm in old ones. 2. The thickness of basal lamina of parietal cells of Bowman's capsule is 187.5 nm in young rats, 914.0 nm in adult ones, and 2850.0 nm in old ones. 3. The numbers of the slit pores of basal lamina are reduced during aging, 30.3 slit pores/$10{\mu}m$ in adult ones, and 24.2 slit pores/$10{\mu}m$ in old ones. 4. Accumulation of dense intracytoplasmic filamentous material in the parietal cells of Bowman's capsule is increased in the vicinity of the basal lamina during aging. The proximal tubule-like epithelial cell in Bowman's capsule is observed at one glomerulus in a young rat. 5. The endothelial cells are edematous and form balloon-like structure protruding into capillary lumen in young and old rats. 6. Cytoplasm of the podocyte shows a variety of alteration during aging, such as swelling of mitochondria and of endoplasmic reticulum, and increase of microtubules, microfilaments, lysosomes and lamellated myelin structures, etc. Accumulation of dense intracytoplasmic material in the foot processes is increased in the vicinity of the basal lamina during aging. The podocytic membrane-like structures are seen in young and o]d rats. 7. The mesangial matrices and mesangial cells are increased during aging, and slight swelling of endoplasmic reticulum and Golgi cisternae in young and old rats.

• 치과방사선
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• 제26권2호
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• pp.75-90
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• 1996

The purpose of this study was to observe microscopic change of salivary gland tissue, which is a cause of xerostomia in diabetic condition; for this target, the author injected streptozotocin 0.1ml/100 gm b.w. on the rat, Sprague Dawley, to induce diabetes, and then observed microscopic changes in parotid gland tissue using light microscopy and electron microscopy. The results were as follows : 1. Parotid gland tissue of the diabetic rat was atrophied or degenerated in lapse of experimental time, but began to repair from 14 days after diabetic induction. 2. In the basal lamina of the vessel of parotid gland tissue in the diabetic rat, lamina lucida was discontinued and lamina densa was increased in thickness, but the number of capillary was gradually increased and dilated. 3. In acinic and intercalated ductal cells of parotid gland in the diabetic rat, changes of mitochondria, RER, secretory granule, free ribosome were prominent. In conclusion, the present study demonstrated that degenerative changes of the parotid gland tissue were due to not completely thickening of the basal lamina of vessels, but many other causal factors, because thickness of the basal lamina of vessels was not related with degenerative changes.

• Applied Microscopy
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• 제27권3호
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• pp.321-332
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• 1997

The dermal papilla is known to playa major role in influencing the form and dynamics of the hair follicle, which probably involves regulatory substances crossing the basal lamina. But little is known about the junctions between the dermal papilla and the surrounding epithelial cells of the hair bulb, or between the connective tissue and the epithelial cells on the outside of the hair follicle. This study was performed to identify the ultrastructural differences between dermoepidermal junction of the skin and connective tissue-epithelial junctions on the outside of the hair follicle and around the dermal papilla of normal anagen hair follicles in the human fetal scalp skin. Electron microscopic findings of dermoepidermal junction in scalp skin showed that basal lamina was very irregular and undulated, and it contained many attachment plaques of hemidesmosomes with sub-basal dense plates, tonofilaments, and anchoring filaments. Also invaginations of plasma membrane of basal keratinocytes were seen. There were clear differences both on the outside of the follicle and around the dermal papilla as compared with similar junction in the skin. In particular, neither hemidesmosomes nor tonofilaments, as seen in dermoepidermal junction, were observed in the dermal papilla. Also attachment plaque, sub-basal dense plate and anchoring filaments were not observed at the junction on the outside of the follicle and the dermal papilla. There were some differences between connective tissue-epithelial junctions on the outside of the hair follicle and around the dermal papilla, ie, smoothness of basal lamina and orthogonal arrangement of collagen fibers were seen in the outside of hair follicle, but not in the dermal papilla. These results indicate that the mechanical connection between the hair follicle and the connective tissue component is much weaker than that between the corresponding components in skin, and it reflects the dynamic processes during the anagen phase of the hair follicle compared to the relatively permanent state of the epidermis.

• Applied Microscopy
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• 제27권4호
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• pp.391-402
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• 1997

본 연구는 고단백뇨를 보이는 신장질환모델마우스(FGS/Kist mouse)의 사구체에 대한 미세구조학적 변화를 관찰하고자 수행하였다. 전반적으로 사구체내의 모세혈관은 주변부의 혈관사이세포(mesangial cell) 부위의 확장으로 인하여 관강이 좁아져 있었으며 관강내에는 점액양 물질들이 흔히 관찰되었다. 또한 사구체바닥판(basal lamina)이 모세혈관 사이의 공간쪽으로 돌출되어져 있는 소견들도 볼 수 있었다. 혈관사이세포 부위에는 주변부와 뚜렷이 구분되는 전자밀도가 높은 물질들이 많이 차여져 있었다. 한편, 사구체바닥판에 인접하여 존재하고 있는 발세포들(podocytes)은 세포질내 많은 공포들이 관찰되었고, 발세포들이 내고 있는 발세포발(foot process)들은 서로 융합되거나 소실되어 매우 불규칙적인 소견을 보였다.

• Animal cells and systems
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• 제1권3호
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• pp.507-513
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• 1997

Sequential observations of binding patterns and structural effects of Bacillus thuringiensis var. kurstaki were made on fat body tissue of the fall webworm, Hyphantria cunea Drury. Fat body was cultured in vitro in the presence of purified 62 kDa endotoxin and then examined for protein synthesis and the localization of membrane-bound toxin detected by an antibody against the 62 kDa endotoxin. Protein synthesis was mostly inhibited at concentrations of 15 ${\mu}$g/ml and higher. Immunocytochemical observations suggest that the toxin binds to all exposed basal lamina surrounding the fat body without apparent specificity. The cytopathic effect delectable by scanning electron microscope is disintegration rather than cell swelling. The basal lamina bound toxin was eventually detached from the fat body and followed by an extrusion of cell contents like lipid granules.

• 대한기관식도과학회지
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• 제1권1호
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• pp.64-68
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• 1995

Vocal folds injury from vocal abuse is important topics of phonosurgery. Recent advances in diagnostic equipment, phonosurgery and speech analysis equipment have provided a lot of Information about fine movement of the vocal folds. However, predicting the reaction of the vocal folds to phonatory trauma remains difficult. The vocal folds need to withstand great vibratory and shearing stress and anchoring fibers of basement membrane Bone play a role in maintaining structural integrity of histologically different epidermis and superficial layer of lamina propria(cover of vocal folds). The purpose of this study is to demonstrate the changes of anchoring fibers in vocal polyp using transmission electron microscope. Various defects were observed : a irregular thickening of basement membrane, a near absence of normal anchoring fiber, a lot of electron dense material in superficial layer of lamina propria, a destruction of hemidesmosome and many vesicles carrying electron dense material In basal keratinocyte. These observations were suggestive of a hyperactivity of basal keratinocyte of vocal folds epithelium in response to vibratory stress.

• International Journal of Oral Biology
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• 제34권3호
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• pp.131-135
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• 2009

The sublingual locus has recently received great attention as a delivery site for various immunotherapies, including those that induce allergen-specific tolerance, and for vaccines that generate protective immunity. To further understand the immune functions of the human sublingual mucosa, we characterized the distribution of various immunocytes therein by immunohistochemistry. We identified professional antigen presenting cells (APCs), including Langerhans cells (LCs) and macrophages. $CD1a^+$ and $langerin^+$ LCs were further found to be distributed in the basal and supra-basal layers of the epithelium, and macrophages were identified in the lamina propria. HLA-$DR^+$ cells were observed in both the epithelium and the lamina propria, which mirrors the tissue distribution of LCs and macrophages within these tissues. $CD3^+$, $CD4^+$, and $CD8^+$ T cells were found to be distributed along the basal layer of the epithelium and also in the lamina propria. Although B cells, plasma cells, and $Foxp3^+$ regulatory T cells (Tregs) were only occasionally observed in the human sublingual mucosa in the absence of inflammation, they did show enrichment at inflammatory sites. Hence, we have further elucidated the immune cell component distribution in human sublingual mucosa.

• 한국동물학회지
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• 제28권1호
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• pp.44-59
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• 1985

가옥내에 서식하는 바퀴 (Blattella germanica Linne)의 後腸을 回腸과 直腸部로 구분하여, 그들을 구성하는 上皮細胞의 微細構造를 관찰하였다. 回腸은 扁平 혹은 立方型의 細胞들이 單層上皮를 만들며 그 內面은 cuticular intima로 덮여 있었다. 細胞들은 細胞器官이 고르게 발달하여 吸收細胞의 形態를 하였으며, 細胞質內에서 동심원적인 環狀構造를 하는 層板狀 結晶體, "spherites"가 많이 관찰됨이 특징이었다. 直腸은 圓柱狀인 圓柱狀吸收細胞의 尖端部原形質膜은 微細絨毛로 변형되었고, 側部原形質膜은 약 200$\\AA$간격으로 심하게 주름이 잡혔으며 그 사이에는 크게 발달한 mitochondria가 밀착하여 mitochondrial-scalarifrom complexes를 형성하였다. 그리고 인접 細胞間에는 많은 細胞間隙을 만들며, 그 사이 사이에는 septate junction과 desmosomes 등으로 연접된 것이 여러 곳에서 관찰되었다. 基底細胞는 납작한 杯모양으로서 細胞質의 電子密度가 높아 검게 보였으며, 그 내에는 Golgi complex, endoplasmic reticulum 그리고 mitochondria 등 細胞器官들이 발달되었고 膜의 구조 등으로 미루어 보아 역시 吸收機能을 가진 細胞임을 확인하였다. 한편 直腸의 上皮層과 hemolymph 사이에서 壁의 역할을 하는 basal sheath가 基底面을 따라 평행하게 부착되어 있고, 그 basal sheath와 근육층 사이에는 結合組織이 넓은 subepithelial space를 만들어서 그 내에는 많은 氣官小支들과 밀도가 높은 顆粒들을 갖는 軸索이 분포하고 있었다.

• Applied Microscopy
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• 제35권3호
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• pp.177-186
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• 2005

장수풍뎅이 (Allomyrina dichotoma; Dynastidae; Coleoptera)를 사육하여 종령 유충의 전장 상피를 구성하는 세포들에 대한 미세구조를 전자현미경으로 관찰하였다. 상피조직은 편상피 세포들이 단층의 점막상피를 이루었으며, 그 내면은 얇은 큐티클 층으로 덮여 있었다. 상피세포의 유리면 원형질막은 미세융모로 변형되었고, 기저 원형질막은 기저막주름이 잡혀 "canaliculi"를 만들며 크게 발달한 사립체와 접해 있었다. 핵은 크게 발달하였고 세포질 내에는 발달한 사립체가 산재해 있었으며, 지질 및 단백질 과립과 글리코겐 입자들이 많이 축적되었다. 상피의 기저막은 여러겹으로 된 기저초를 이루고 있으며, 기저막내 또는 섬유상 결합조직인 점막하조직내에는 기관지세포와 신경이 뻗어 있었다. 그리고 점막하 조직 바깥쪽에는 환상근과 종주근이 관찰되었다.