• Journal of Microbiology and Biotechnology
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• v.18 no.5
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• pp.845-851
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• 2008

TolC is an outer membrane porin protein and an essential component of drug efflux and type-I secretion systems in Gram-negative bacteria. TolC comprises a periplasmic $\alpha$-helical barrel domain and a membrane-embedded $\beta$-barrel domain. TdeA, a functional and structural homolog of TolC, is required for toxin and drug export in the pathogenic oral bacterium Actinobacillus actinomycetemcomitans. Here, we report the expression of the periplasmic domain of TdeA as a soluble protein by substitution of the membrane-embedded domain with short linkers, which enabled us to purify the protein in the absence of detergent. We confirmed the structural integrity of the TdeA periplasmic domain by size-exclusion chromatography, circular dichroism spectroscopy, and electron microscopy, which together showed that the periplasmic domain of the TolC protein family fold correctly on its own. We further demonstrated that the periplasmic domain of TdeA interacts with peptidoglycans of the bacterial cell wall, which supports the idea that completely folded TolC family proteins traverse the peptidoglycan layer to interact with inner membrane transporters.

• Journal of the Korea Institute of Military Science and Technology
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• v.10 no.3
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• pp.208-216
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• 2007

As the propellant mass is being accelerated out of the gun chamber along with the projectile, a continuous pressure gradient exists between the end of chamber and the base of the projectile. For this reason, the base pressure-travel curve is very important to design a conventional gun barrel in the interior ballistics, but it is not obtained briefly by empirical or theoretical method. In this paper, a simple relation between chamber pressure and base pressure was determined by the factor of base pressure(Cb) obtained from the experimental method. The simple relation gives a reasonable prediction for the reduction of pressure between the breech and the base of projectile owing to the axial gradient in the gun tube. The predictions have been validated by the infrared screen sensor and the PRODAS(PROjectile Design and Analysis System) for interior ballistic systems. Therefore, the base pressure-travel curve could be calculated from the chamber pressure measured by piezoelectric sensor. The base pressure-travel curve obtained from the simple relation offers initial information to gun barrel designer and is used for calculation of muzzle velocity.

• Transactions of the Korean Society of Mechanical Engineers B
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• v.20 no.6
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• pp.1994-2004
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• 1996

Numerical calculation was applied to supersonic under-expanded jets, and compared with the results of a linear theory and other experiments. TVD difference scheme was employed to solve 2-dimensional and axisymmetric inviscid Euler equation. This paper aims to explore the effects of angle of divergence and design Mach number of nozzle on the structure of under-expanded jets. The angle of divergence was varied from 0 to 20 deg. The results show that the length of the first cell of the under-expanded jets decreases and Mach disk generates at lower nozzle pressure ratio, if the angle of divergence or design Mach number of nozzle increases. The distance from the nozzle exit to Mach disk in 2-dimensional jets becomes much larger than that of axisymmetric jets, and the widths of the jet boundary and the barrel shock wave are also larger than that of axisymmetric jets. Calculation results indicate that the configuration of the under-expanded jets is strongly dependent on the nozzle pressure ratio.

• Food Science and Biotechnology
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• v.17 no.6
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• pp.1191-1196
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• 2008

The antibacterial and antioxidant potentials of extruded ginseng extract (EGE) with 60% ethanol and methanol were investigated. The inhibitory activity of the EGE in Gram-positive bacteria was significantly higher than in Gram-negative bacteria. Higher antibacterial activity was observed with methanol ginseng extract when moisture content and barrel temperature were 20% and $115^{\circ}C$, respectively, that diameter of inhibition zone at 1,500 mg/mL was $15.40{\pm}0.13\;mm$ for Bacillus subtilis and $9.31{\pm}0.05\;mm$ for Salmonella typhimurium. The amount of total phenolics was highest in extruded ginseng at 20% moisture content and $115^{\circ}C$ barrel temperature. Especially, a positive correlation was observed between the total phenolic content and antioxidant activity of the extracts. In the 2,2-diphenyl-1-picryhydrazyl radical (DPPH) system, all tested extract of extruded ginseng at 20% moisture content exhibited very strong antioxidant properties when compared to red ginseng with percent scavenging effect of 23-35% at 20mg/mL. In conclusion, it can be said that the extracts of extruded ginseng could be used as natural antimicrobial and antioxidant agents in the food preservation.

• Journal of Welding and Joining
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• v.28 no.2
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• pp.60-65
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• 2010

Although two dimensional axi-symmetric modeling is useful for calculating the residual stresses of a cylindrical weldment such as a core barrel, this conventional axi-symmetric modeling can not express the behavior of shrinkage well in the locally heated weld zone. New technique of two dimensional axi-symmetric modeling using a virtual fixture is suggested to simulate the behavior of dimensional changes in the weld zone during the heating period of the welding. The virtual fixture in the model has a role to restrain the expansion of the high temperature heated region, which simulates equivalent intrinsic restraint effect of the weldment. In the restraint condition of the virtual fixture above the critical yield strength, the calculated shrinkages by using the suggested axi-symmetric model agreed well with those measured in a welded mock-up. The calculated residual stresses by using the suggested axi-symmetric model also agreed well with those calculated by using conventional axi-symmetric model which has beenused for calculating residual stresses in the weldment.

• Current Optics and Photonics
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• v.4 no.6
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• pp.500-508
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• 2020

The rifling angle of artillery is an important parameter, and its determination plays a key role in the stability, hit rate, accuracy and service life of artillery. In this study, we propose an optical measurement method for the rifling angle based on angle error correction. The method is based on the principle of geometrical optics imaging, where the rifling on the inner wall of the artillery barrel is imaged on a CCD camera target surface by an optical system. When the measurement system moves in the barrel, the rifling image rotates accordingly. According to the relationship between the rotation angle of the rifling image and the travel distance of the measurement system, different types of rifling equations are established. Solving equations of the rifling angle are deduced according to the definition of the rifling angle. Furthermore, we added an angle error correction function to the method that is based on the theory of dynamic optics. This function can measure and correct the angle error caused by the posture change of the measurement system. Thus, the rifling angle measurement accuracy is effectively improved. Finally, we simulated and analyzed the influence of parameter changes of the measurement system on rifling angle measurement accuracy. The simulation results show that the rifling angle measurement method has high measurement accuracy, and the method can be applied to different types of rifling angle measurements. The method provides the theoretical basis for the development of a high-precision rifling measurement system in the future.

• Computers and Concrete
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• v.31 no.5
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• pp.371-384
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• 2023

Precast roofing systems employing prestressed elements often serve as smart structural solutions for the construction of industrial buildings. The precast concrete elements usually employed are highly engineered, and often consist in thin-walled members, characterised by a complex behaviour in fire. The present study was carried out after a fire event damaged a precast industrial building made with prestressed beam and roof elements, and non-prestressed curved barrel vault elements interposed in between the spaced roof elements. As a consequence of the exposure to the fire, the main elements were found standing, although some locally damaged and distorted, and the local collapse of few curved barrel vault elements was observed in one edge row only. In order to understand and interpret the observed structural performance of the roof system under fire, a full fire safety engineering process was carried out according to the following steps: (a) realistic temperature-time curves acting on the structural elements were simulated through computational fluid dynamics, (b) temperature distribution within the concrete elements was obtained with non-linear thermal analysis in variable regime, (c) strength and deformation of the concrete elements were checked with non-linear thermal-mechanical analysis. The analysis of the results allowed to identify the causes of the local collapses occurred, attributable to the distortion caused by temperature to the elements causing loss of support in early fire stage rather than to the material strength reduction due to the progressive exposure of the elements to fire. Finally, practical hints are provided to avoid such a phenomenon to occur when designing similar structures.

• Journal of Korean Ophthalmic Optics Society
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• v.20 no.1
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• pp.1-7
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• 2015

Purpose: For optimizing properties of final glasses frame, the aim of this study is to examine the correlation of processing conditions and properties of cellulose acetate (CA) sheets through the investigation of properties of CA sheets prepared under processing steps. Methods: The properties of CA sheets were investigated in terms of different glasses frame processing conditions, bending process, barrel process, and ultrasonic cleaning process. CA sheets prepared through the sequential processing were examined by various analysis: gloss, mechanical properties, thermal properties. Results: After barrel process, hardness and tensile strength of CA sheet were increased. However, bending strength and impact strength were decreased. It is suggested the CA sheet had became rather stiff state (brittle). Also, in degradation temperature region of plasticizer, about 3% of reduction in plasticizer weight was confirmed upon TGA analysis. Conclusions: Glasses frame process, especially in the barrel process have a profound influence on the properties of CA sheet owing to reduction of total amount of plasticizer.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.21 no.9
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• pp.120-127
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• 2020

This study examined the operation of a pressure gauge for measuring the internal pressure of a barrel. During the ammunition performance evaluation test, the internal pressure of the barrel was measured mainly using a copper crusher gauge. The internal piezo gauge (IPG) was used to replace the pressure measurement method, and the test results were analyzed to verify the operability of the IPG. For verification, actual shooting test data were used, and a t-test was used to analyze the results. The 155MM KH179 and M119 series propellant were used for the test equipment and ammunition. The results confirmed that there was no difference between the mean values of the two data results at a significance level of 5% for the data values between the copper gauge and the IPG. The use of the IPG was verified by replacing the existing copper gauge to measure the internal pressure of the barrel, and it was confirmed that the application width could be widened in terms of diversity of test measurement methods. Based on the results of this test, it can be applied to the compression measurement test of various types of ammunition in addition to the propellant charges.

• Korean Journal of Food Science and Technology
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• v.30 no.5
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• pp.1024-1028
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• 1998

Crystalline structure of the extrudate of high amylose corn starch was studied by X-ray diffractometer and $^{13}C\;NMR$. The X-ray diffraction crystal ratio of the extrudates (barrel temperature $100^{\circ}C$ )of high amylose corn starch slightly increased from 6.08% to 8.37% by increasing feed moisture content from 25% to 45%. But extrudates of high amylose corn starch showed similar crystal ratio on various extrusion conditions. Extrudates of high amylose corn starch (feed moisture content 20%, barrel temp $140^{\circ}C$) showed more enlarged crystal structure than that of non-extrudates. The perpendicular distance of crystal increased by extrusion. Crystal ratio was changed from $6.3{\sim}8.3%$ to $4.5{\sim}5.8%$ during storage at $4^{\circ}C$. Starch configuration was examined with $^{13}C\;NMR$. Double helical content was measured by $^{13}C\;NMR$ method. The highest double helical content (60%) was obtained from high amylose corn starch extrudate (barrel temp.: $100^{\circ}C$, feed moisture content 45%). Double helical contents and resistant starch (RS) yield (pancreatin) were positively correlated. However, double helical content of the extrudates was not changed by the storage at $4^{\circ}C$.