• Title/Summary/Keyword: bacterial spot

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Development of Control Method for Strawberry Bacterial Angular Spot Disease (Xanthomonas fragariae) (딸기 세균모무늬병(Xanthomonas fragariae)의 방제를 위한 약제 선발)

  • Kim, Da-Ran;Gang, Geun-Hye;Cho, Hyun ji;Myung, Inn-Shik;Yoon, Hae-Suk;Kwak, Youn-Sig
    • The Korean Journal of Pesticide Science
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    • v.19 no.3
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    • pp.287-294
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    • 2015
  • In Korea, Strawberry bacterial angular leaf spot disease was first reported in 2010. The disease of strawberry caused serious problem for strawberry producers and export. To field test, we applied the bactericides in June and October as nursery stage and cultivation stage, respectively. In nursery stage with high temperature condition, Oxolinic acid (97.2%) showed significant control effect. In cultivation stage with low temperature condition is soil sterilization and soil non- sterilization the control effect. In cultivation stage in soil sterilization stage Validamycin (93.3%) showed reliable control effect against the disease. Also, soil non-sterilization stage with low control effect of Validamycin (80%) showed control effect the lower than a soil sterilization stage.

Control Efficacy of Different Types of Chemicals with Different Spraying Schedules on Plum Bacterial Black Spot (약제별 및 살포시기별 자두 검은점무늬병 방제효과)

  • Ryu, Young Hyun;Lee, Joong Hwan;Kwon, Tae Young;Kim, Seung Han;Kim, Dong Geun
    • Research in Plant Disease
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    • v.18 no.4
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    • pp.349-353
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    • 2012
  • Xanthomonas arboricola pv. pruni causes black spot symptom on fruit of plum, resulting in yield loss by reduction of marketable fruit production. To develop an effective control program, some chemicals were sprayed in various scheme during dormant season and growing season after blooming period. Copper-based chemicals were sprayed during dormant season and antibiotic-based chemicals were sprayed during fruit growing season. Sprays of antibiotic-based chemicals in growing season was more effective than copper-based chemicals sprays in dormant season. Three applications of antibiotic-based chemicals in 10 days interval starting 10 days after full blooming controlled disease incidence as much as 93%, whereas applications of copper-based chemicals in dormant season controlled 26-42%. Antibiotic-based chemicals application starting 10 days after full blooming was more effective than starting 20 or 30 days after full blooming.

Occurrence of Bacterial Black Spot on Plum by Xanthomonas aboricola pv. pruni and It's Pathogenicity on Varieties of Some Stone Fruits (Xanthomonas aboricola pv. pruni에 의한 자두 검은점무늬병의 발생과 핵과류 과수 품종에 대한 병원성)

  • Ryu, Young-Hyun;Lee, Joong-Hwan;Kwon, Tae-Young;Kim, Seung-Han;Kim, Dong-Geun
    • Research in Plant Disease
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    • v.18 no.1
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    • pp.40-44
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    • 2012
  • Xanthomonas arboricola pv. pruni is the causal agent of bacterial black spot disease on some stone fruits, e.g. peach, plum and apricot. To evaluate pathogenicity of Xanthomonas arboricola pv. pruni strain from plum, inoculum of the isolated strain was spray inoculated to fruits and leaves of apricot, Japanese apricot and plum. Apricot and Japanese apricot showed severe black spot symptoms on fruits and shot hole symptoms on leaves. In case of apricot, about fifty percent of fruits did not grow and dropped by hypersensitive reaction to spray infection. Plum, cv. Formosa was very susceptible, showing severe black injury lesions on fruits and cankers on leaves and new twigs. On the other hand, plum cv. Daesukjosaeng, was highly resistant. Fruits of several plum cultivars such as Formosa and Chuhee were severely infected at natural infected orchards by X. arboricola pv. pruni. Where as those of Daesukjosaeng, Taeyang, Soldam and Hongrogen were moderately infected.

Diagnosis and Control of Major Leaf Diseases on Kiwifruit in Korea (키위 잎 주요 병 진단 및 방제)

  • Kim, Gyoung Hee;Koh, Young Jin
    • Research in Plant Disease
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    • v.24 no.1
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    • pp.1-8
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    • 2018
  • Bacterial diseases such as bacterial canker and bacterial leaf spot and fungal diseases such as gray mold, powdery mildew, side rot and leaf spots are major diseases damaging leaves of kiwifruit in Korea. In this review, we summarize symptoms and epidemiological characteristics of the major bacterial and fungal leaf diseases of kiwifruit and propose proper control methods of the diseases that can be practically utilized at the farmers' kiwifruit orchards in order to prevent the diseases on the basis of our research works and field experiences and important research products conducted during the last three decades in the world.

Incidence Rates of Major Diseases of Kiwiberry in 2015 and 2016

  • Kim, Gyoung Hee;Kim, Deok Ryong;Park, Sook-Young;Lee, Young Sun;Jung, Jae Sung;Koh, Young Jin
    • The Plant Pathology Journal
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    • v.33 no.4
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    • pp.434-439
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    • 2017
  • Incidence rates of diseases in kiwiberry orchards were investigated monthly from late June to late September in Gwangyang and Boseong in 2015 and 2016. The impact of postharvest fruit rot was investigated during ripening after harvest. Bacterial canker was only observed on one single tree in 2015, but black rot, powdery mildew, leaf spot and blight, and postharvest fruit rot diseases were problematic throughout the study period in both 2015 and 2016. Incidence rates of the diseases varied with kiwiberry cultivar, region and sampling time. Incidence rates of powdery mildew, leaf spot and blight diseases increased significantly during the late growing stages near fruit harvest, while black rot peaked in late August. Incidence rate of postharvest fruit rot on fruit without fruit stalks was less than half of fruit with fruit stalks, regardless of kiwiberry cultivars. Among the four cultivars, Mansu was relatively resistant to black rot and postharvest fruit rot diseases. In our knowledge, this is the first report of various potential pathogens of kiwiberry in Korea.

Comparative Proteome Analysis of Celastrol-Treated Helicobacter pylori

  • Kim, Sa-Hyun
    • Biomedical Science Letters
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    • v.23 no.4
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    • pp.395-401
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    • 2017
  • Various preclinical and clinical trials have been conducted the efficacy of celastrol. In data presented in the current manuscript is the first trial to inhibit Helicobacter pylori with celastrol. In this study, the quantitative change of various H. pylori proteins including CagA and VacA by the anti-bacterial effect of celastrol was determined. The anti-H. pylori effects of celastrol was investigated by performing 2-dimensional electrophoresis and additional supporting experiments. After 2-dimensional electrophoresis analysis, spot intensities were analyzed and then each spot was identified using matrix assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF-MS) or peptide sequencing using Finnigan LCQ ion trap mass spectrometer (LC-MS/MS). The results show that celastrol has multiple effects on protein expression in H. pylori.

Real-time PCR Quantification of White Spot Syndrome Virus (WSSV) and Hepatopancreatic Parvovirus (HPV) Loads in Shrimp and Seawaters of Shrimp Ponds on the West Coast of South Korea

  • Jang, In-Kwon;Gopalakannan, Ayyaru;Suriakala, Kannan;Kim, Jong-Sheek;Kim, Bong-Rae;Cho, Yeong-Rok;Meng, Xian-Hong;Seo, Hyeong-Chul
    • Fisheries and Aquatic Sciences
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    • v.11 no.4
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    • pp.195-204
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    • 2008
  • Viral diseases are major emerging problems of shrimp that have affected the production, and even complete losses for shrimp farms. In this study, we developed a sensitive TaqMan real-time PCR method to quantify white spot syndrome virus (WSSV) and hepatopancreatic parvovirus (HPV) in the shrimp and pond water in which fleshy shrimp, Fenneropenaeus chinensis, and Pacific white shrimp, Litopenaeus vannamei, are reared. WSSV and HPV in pond seawaters ranged from $1.65{\times}10^3$ to $2.43{\times}10^9$ and from 0 to $4.43{\times}10^5$ copies/L of seawater, respectively. Of 20 ponds analyzed, all pond water and shrimp were positive for WSSv. L. vannamei showed higher susceptibility to WSSV than F chinensis. HPV was detected only in the pond water for F chinensis. In shrimp tissue, however, HPV was found in both species, with 23-times higher infection rate in F chinensis than L. vannamei. The total bacterial counts in the pond water ranged from $2.23{\times}l0^3$ to $1.98{\times}l0^5\;CFU/mL$. The variations in total bacterial count for each pond appeared to correlate to the variations of the WSSV load. Statistical analysis indicated that there was no significant difference (P>0.05) between the WSSV load in pond water and shrimp, and there was no relationship between total bacterial load and viral load in the pond water. However, a significant difference (P<0.01) was found between HPV load and L. vannamei and F chinensis pond water.

Occurrence of Leaf Spot Disease on Watermelon Caused by Pseudomonas syringae pv. syringae (Pseudomonas syringae pv. syringae에 의한 수박 잎점무늬병의 발생)

  • Park, Kyoung-Soo;Lee, Ji-Hye;Kim, Young-Tak;Kim, Hye-Seong;Lee, June-woo;Lee, Hyun-Su;Lee, Hyok-In;Cha, Jae-Soon
    • Research in Plant Disease
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    • v.27 no.4
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    • pp.180-186
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    • 2021
  • Typical bacterial symptoms, water-soaking brown and black leaf spots with yellow halo, were observed on watermelon seedlings in nursery and field of Gyeongnam and Jeonnam provinces. Bacterial isolates from the lesion showed strong pathogenicity on watermelon and zucchini. One of them was rod-shaped with 4 polar flagella by observation of transmission electron microscopy. They belonged to LOPAT group 1. The phylogenical trees with nucleotide sequences of 16S rRNA and multi-locus sequencing typing with the 4 house-keeping genes (gapA, gltA, gyrB, and rpoD) of the isolates showed they were highly homologous to Pseudomonas syringae pv. syringae and grouped together with them, indicating that they were appeared as P. syringae genomospecies group 1. Morphological, physiological, and genetical characteristics of the isolates suggested they are P. syringae pv. syringae. We believe this is the first report that P. syringae pv. syringae caused leaf spot disease on watermelon in the Republic of Korea.

Selection in the Generations after Backcrosses of the Crosses for Incorporation of Resistance to Bacterial Spot into Korean Local Cultivars of Pepper (고추 재래품종에 더뎅이병(반점세균병) 저항성을 도입하기 위한 여교잡 이후 세대에서의 선발)

  • 김병수;김점순;임양숙
    • Korean Journal Plant Pathology
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    • v.12 no.4
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    • pp.437-444
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    • 1996
  • 경북지역 재래종에 더뎅이병 저항성을 도입하기 위하여 작성한 칼미초$\times$PI271322, 수비초$\times$PI271322 조합의 BC\ulcornerF\ulcorner와 BC\ulcornerF\ulcorner세대로부터 BC\ulcornerF\ulcorner와 BC\ulcornerF\ulcorner세대까지 선발과 자식을 실시하였다. 이 결과 각 재래종의 원예적 특성에 PI271322에 들어있는 과민반응형 저항성 유전자 Bs\ulcorner와 비과민형의 일반저항성을 함께 가지는 계통이 육성되었다.

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