• 한국잠사곤충학회지
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• 제37권1호
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• pp.86-91
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• 1995

축엽세균병으로 알려진 피해뽕나무에서 진균인 Fusarium spp. 균이 분리되어 이를 구명하고 그 피해정도를 조사한 결과 1. 신소썩음증상은 세로로 긴 갈색의 병무늬가 시간이 경과할 수록 암갈색으로 변하고 흰곰팡이가 생기며, 축엽세균병증상은 잎이 말리고 신소에 수침상의 병반과 bacterial ooze가 보인다. 그러나 시간이 경과하면 구분이 어렵다. 2. 신소썩음증상의 피해율은 7.5%, 축엽세균병의 피해율은 4.4%였다. 3. Fusarium spp. 균의 분리는 신소썩음증상에서 높았고, Pseudomonas균의 분리는 축엽세균병 증상에서 많았다. 또한 병원성도 같은 경향을 보였다. 4. 수원 등 3지역 모두에서 부위별 병원성 Fusarium spp. 균의 분리율은 병반에서 30cm 부위에서 보다 병진전부위에서 더 높았다. 5. 수원 등 3지역 모두에서 개량뽕의 신소썩음증상 피해가 가장 많았다. 그러나 청올뽕의 신소썩음증상 피해는 조사 품종중에 가장 적었다.

• 미생물학회지
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• 제23권1호
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• pp.43-48
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• 1985

철저한 혐기적 방법으로 배양분리한 결과 편성 혐기성세균의 분리율이 높아졌고, 검체당 평균 3.3주가 분리되었다. 혈액배지인 NATB배지와 BA배지에서 비혈액배지인 NAT배지와 GAM배지에서 보다 세균 분리효과가 우수했다. NATB배지에는 혐기성 그람 양성 구균에 좋은 분리효과를 나타내었고, 그람 음성 간균은 15주 분리되었다. 이 중 Bacteroides가 12주로서 가장 많이 존재하는 균종이었다.

• 한국미생물생명공학회:학술대회논문집
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• 한국미생물생명공학회 2000년도 추계 학술대회
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• pp.109-117
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• 2000

Bacterial community structure composing enhanced biological phosphorus removal (EBPR) activated sludge was analyzed phylogenetically by cloning 165 rDNA after direct DNA extraction. Then, this result was compared with 165 rDNA sequences of randomly isolated bacterial species. The results clearly showed that there are no coincidence between the sequences retrieved directly from activated sludge and those of isolated strains, suggesting that many important bacteria are hidden in activated sludge because of the difficulty in isolation and culture of them.

• Journal of Microbiology and Biotechnology
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• 제14권4호
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• pp.730-736
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• 2004

This study aims at characterizing the bacteriophages isolated from activated sludge performing enhanced biological phosphorous removal (EBPR) to understand the interactions between the phage-host system and bacterial community. Sixteen bacterial isolates (E1-E16) were isolated as host bacterial strains from EBPR activated sludge for phage isolation. Forty bacteriophages based on their plaque sizes (2 plaques on E4, 4 on E8, 11 on E10, 5 on E14, 18 on E16) were obtained from filtered supernatant of the EBPR activated sludge. Each bacteriophage did not make any plaque on bacterial strains tested in this study except on its own host bacterial strain, respectively, indicating that the bacteriophages are with narrow host specificity. However, fourteen of the forty bacteriophages obtained in this study lost their virulent ability even on their own host bacteria. All of the lytic phages showed similar one-step growth patterns and had long latent period (about 9 hours) to reproduce their phage particles in their host bacterial cells. On the other hand, their probable burst sizes (6 to 48 per host cell) were large enough to actively lyse their host bacterial cells. Therefore, it could be implied that bacteriophages are also important members of the microbial community in EBPR activated sludge, and lytic phages directly decrease the population size of their host bacterial groups in EBPR activated sludge by lysis.

• 한국미생물·생명공학회지
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• 제20권1호
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• pp.110-113
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• 1992

In the screening process of anti- or desmutagenic substance from the various microbial metabolites with the method of Ames and Rec-assay, a desmutagenic substance producing bacterial strain which inactivates the mitomycin C-induced mutagenicity was isolated and identified as Psudomonas sp. AM-10.

• 대한족부족관절학회지
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• 제25권2호
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• pp.100-107
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• 2021

Purpose: The present study aimed to develop guidelines regarding initial choice of antibiotics for diabetic foot ulcers (DFU) by investigating bacterial isolates. Materials and Methods: This study included 223 DFU patients that visited a single tertiary hospital and underwent bacterial culture between January 2016 and February 2020. The study was conducted in two parts: 1) to compare bacterial isolates and wound healing according to comorbidities such as chronic kidney disease (CKD) and peripheral artery disease (PAD), and 2) to compare bacterial isolates according to wound depth using the Wagner classification. Results: Of the 223 patients, 43 had CKD (group A), 56 had PAD (group B), 30 had CKD and PAD (group C), and 94 had none of these comorbidities (group D). The isolation rate for multidrug-resistant gram-negative bacteria (MRGNB) and gram-negative to gram-positive bacteria ratio were highest in group C (p=0.018, p=0.038), and the proportion that achieved wound healing was lowest in group C (p<0.001). In the second part of the study, subjects were classified into 5 grades by wound depth using the Wagner classification; 13 grade I, 62 grade II, 60 grade III, 70 grade IV, and 17 grade V. No significant difference was observed between these grades in terms of isolation rates or gram-negative to gram-positive bacteria ratios. Conclusion: This study suggests antibiotics that cover gram-negative bacteria including MRGNB produces better results in the presence of CKD and PAD and that initial antibiotic choice should be based on the presence of CKD and PAD rather than wound depth.

• 생명과학회지
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• 제8권6호
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• pp.711-722
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• 1998

본 연구는 혈액에서 분리되는 세균의 항균제 감수성 양상을 파악하기 위하여 1993년 10월 1일부터 1996년 9월 30일 사이에 K대학 부속병원의 입원 및 외래환자 13,595명을 대상으로 혈액배양에서 분리된 1,104균주중 716균주를 세균의 균종별, 연령별, 계절별 분리빈도와 항균제 감수성 검사를 실시하여 다음과 같은 결과를 얻었다. 1. 최근 3년간 균분리 양성은 1,104명으로 8.1%의 양성률을 보였다. 연구대상 716균주의 균종별 분리빈도는 E. coli가 29.8% (213주)로 분리율이 가장 높았다. 2. 대부분의 균종은 50세, 60세군 및 2세 이하군에서 분리율이 높았고, S. typhi는 30세군에서 분리율이 높았다. 3. 계절별 분리율은 대부분의 균종이 여름철에 분리율이 높았고, E. coli는 계절에 관계없이 분리되었다. 4. 항균제 감수성은 그람양성 구균의 경우 penicillin계 항균제인penicillin-G에 E. faecalis를 제외한 균에서 높은 내성률을 보였으며, vancomycin과 teicoplanin에는 감수성이 높았다. 그람음성 간균은 amikacin, ciprofloxacin, 및 imipenem에는 감수성이 높았고, ampicillin에는 내성을 보였다. S. typhi는 cephalothin을 제외한 모든 항균제에 100%의 감수성이었다. 이상의 결과에서 균혈증 환자로부터 분리된 세균에는 penicillin계 항균제에 내성인 균주가 많으며 glycopeptide계 항균제와 amikacin, ciprofloxacin 및 imipenem에는 아직 내성이 낮다는 결론을 얻었다.

• Journal of Species Research
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• 제7권1호
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• pp.60-72
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• 2018

Our study aimed to discover indigenous prokaryotic species in Korea. A total of 29 bacterial species in the phylum Proteobacteria were isolated from freshwater and sediment of rivers and brackish zones in Korea. From the high 16S rRNA gene sequence similarity (${\geq}98.8%$) and formation of a robust phylogenetic clade with the closest species, it was determined that each strain belonged to an independent and predefined bacterial species. To our knowledge, there is no official report or publication that has previously described these 29 species in Korea. Specifically, we identified 10, 12, and seven species of eight, 12, and seven genera that belong to classes Alphaproteobacteria, Betaproteobacteria, and Gammaproteobacteria, respectively; all are reported as previously unrecorded bacterial species in Korea. The Gram reaction, colony and cell morphology, basic biochemical characteristics, isolation source, and strain IDs for each are also described.

• Journal of Species Research
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• 제7권1호
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• pp.9-12
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• 2018

In 2016, as part of a larger effort to discover indigenous prokaryotic species in Korea, we isolated the family Deinococcaceae and Planctomycetaceae as unrecorded bacterial species. From the high 16S rRNA gene sequence similarity (>98.5%) and formation of a robust phylogenetic clade with known species, it was determined that each strain was a distinct bacterial species. There are no official reports that these two species have been described in Korea; therefore, the bacterial strains of Deinococcus and Blastopirellula are described for the first time in Korea. Gram reaction, colony and cell morphology, basic biochemical characteristics, and isolation sources are also described in the species description section.

• 대한수의학회지
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• 제48권1호
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• pp.89-92
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• 2008

The pirarucu, Arapaima gigas (body weight = 18.3 kg and total length = 102 cm) which had been reared in one of the private commercial aquaria for exhibition was found dead and submitted for diagnostic examination. A pure bacterial culture was isolated from the kidney, which was enlarged, and contained fluids. Result of the bacterial identification yielded Serratia fonticola. This paper describes the first isolation of S. fonticola from pirarucu.