Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Isolation, Physiological Characterization of Bacteriophages from Enhanced Biological Phosphorus Removal Activated Sludge and Their Putative Role

  • Lee, Sang-Hyon (Institute of Environmental Studies, Graduate School of Frontier Sciences, The University of Tokyo) ;
  • Satoh, Hiroyasu (Institute of Environmental Studies, Graduate School of Frontier Sciences, The University of Tokyo) ;
  • Katayama, Hiroyuki (Institute of Environmental Studies, Graduate School of Frontier Sciences, The University of Tokyo) ;
  • Mino, Takashi (Institute of Environmental Studies, Graduate School of Frontier Sciences, The University of Tokyo)
  • Published : 2004.08.01
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Abstract

This study aims at characterizing the bacteriophages isolated from activated sludge performing enhanced biological phosphorous removal (EBPR) to understand the interactions between the phage-host system and bacterial community. Sixteen bacterial isolates (E1-E16) were isolated as host bacterial strains from EBPR activated sludge for phage isolation. Forty bacteriophages based on their plaque sizes (2 plaques on E4, 4 on E8, 11 on E10, 5 on E14, 18 on E16) were obtained from filtered supernatant of the EBPR activated sludge. Each bacteriophage did not make any plaque on bacterial strains tested in this study except on its own host bacterial strain, respectively, indicating that the bacteriophages are with narrow host specificity. However, fourteen of the forty bacteriophages obtained in this study lost their virulent ability even on their own host bacteria. All of the lytic phages showed similar one-step growth patterns and had long latent period (about 9 hours) to reproduce their phage particles in their host bacterial cells. On the other hand, their probable burst sizes (6 to 48 per host cell) were large enough to actively lyse their host bacterial cells. Therefore, it could be implied that bacteriophages are also important members of the microbial community in EBPR activated sludge, and lytic phages directly decrease the population size of their host bacterial groups in EBPR activated sludge by lysis.

Keywords

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