• Title/Summary/Keyword: bacterial growth

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Effect of R. leguminisarum Pre-incubated with Inducers, Naringenin and Methyl-jasmonate, on Nitrogen Fixation and the Growth of Pea at Different Salinity Levels

  • Lee, Kyung-Dong
    • Korean Journal of Environmental Agriculture
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    • v.27 no.4
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    • pp.362-367
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    • 2008
  • The legume-rhizobia symbiosis is an important source of plant growth and nitrogen fixation for many agricultural systems. This study was conducted to investigate the effects of salinity stress on nitrogen fixation and growth of pea (Pisum sativum L.), which has antimutagenic activities against chemical mutagen, inoculated with R. leguminosarum bv. viciae cultured with additional plant-to-rhizobia signal compounds, naringenin (NA,15 uM), methyl-jasmonate (MJ, 50 uM) or both, under greenhouse conditions. Three salinity levels (0.6, 3.0 and $6.0\;dS\;m^{-1}$) were imposed at 3 days after transplanting and maintained through daily irrigations. Addition of signal compounds under non-stress and stress conditions increased dry weight, nodule numbers, leaf area and leaf greenness. The inducers increased photosynthetic rate under non-stress and stress conditions, by approximately 5-20% when compared to that of the non-induced control treatment. Under stress conditions, proline content was less in plants treated with plant-to-bacteria signals than the control, but phenol content was significantly increased, compared to that of the control. The study suggested that pre-incubation of bacterial cells with plant-to-bacteria signals could enhance pea growth, photosynthesis, nitrogen fixation and biomass under salinity stress conditions.

The Indirect Effects of the Near Infra-Red Light-Treated Materials on Microbial Growth (근적외선을 처리한 생활용품의 향균 효과)

  • Park Kyoung-Hwa;Park Yu-Mi;Seul Kyeung-Jo;Ghim Sa-Youl
    • Microbiology and Biotechnology Letters
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    • v.33 no.3
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    • pp.222-225
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    • 2005
  • Stimulatory effects of near infra-red (NIR) rays radiation have been studied within the limits of photosynthesis, phototaxis, and photodermatology. While most of these studies have been done by direct NIR radiation, we investigated the effects of the NIR rays-treated materials on microbial growth. NIR in wavelength of 1,400${\~}$1,700 nm was applied for different kind of materials. Under fast growing conditions in rich media, materials treated with the NIR rays or not did not show any differences in growth of microorganisms. However, under slow growing conditions in minimal media, data showed that NIR rays-treated cloths and hygienic bands affect negatively on the growth of bacteria (Salmonella enteritidis) and fungi (Candida albicans). In addition, it was estimated that the effect of NIR rays on bacterial growth is kept going on S. enteritidis.

Studies on Growth Characteristics of lactobacillus brevis Isolated from Kimchi - Optimization of Nutrient Composition in Sourdough Media - (김치에서 분리한 Lactobacillus brevis의 생장 특성에 관한 연구( I ) - Sourdough 배지의 영양 조성 최적화 -)

  • 신언환
    • The Korean Journal of Food And Nutrition
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    • v.15 no.3
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    • pp.215-219
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    • 2002
  • Growth characteristics of sourdough lactic acid bacteria was investigated to obtain basic informations for sourdough starter. The optimum temperature and pH on bacterial growth and lactic acid production of Lactobacillus brevis UC-22 in sourdough broth were 35'E and around pH 5.5, respectively. And the optimum concentrations of the carbohydrate sources added to the broths was 2% maltose. The acidity significantly increased during growth by Lactobacillus brevis UC-22 fur 18 hours while pH significantly decreased during growth.

Gibberellins-Producing Rhizobacteria Increase Endogenous Gibberellins Content and Promote Growth of Red Peppers

  • Joo Gil-Jae;Kim Young-Mog;Kim Jung-Tae;Rhee In-Koo;Kim Jin-Ho;Lee In-Jung
    • Journal of Microbiology
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    • v.43 no.6
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    • pp.510-515
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    • 2005
  • The growth of red pepper plants was enhanced by treatment with the rhizobacterium, Bacillus cereus MJ-1. Red pepper shoots showed a 1.38-fold increase in fresh weight (fw) and roots showed a 1.28-fold fw gain. This plant growth-promoting rhizobacterium (PGPR) has been reported to produce gibberellins (GAs). Other GAs-producing rhizobacteria, Bacillus macroides CJ-29 and Bacillus pumilus CJ-69, also enhanced the fw of the plants. They were less effective than B. cereus MJ-1, though. The endogenous GAs content of pepper shoots inoculated with MJ-1 was also higher than in shoots inoculated with CJ-29 or CJ-69. When inoculated with MJ-1, bacterial colonization rate of the roots was higher than that of roots inoculated with CJ-29 or CJ-69. These results support the idea that the plant growth-promoting effect of the bacteria also positively related with the efficiency of root colonization by the bacteria. In addition, we identified the major endogenous GAs of the red pepper as originating from both the early C-13 hydroxylation and the early non C-13 hydroxylation pathways, with the latter being the predominant pathway of GA biosynthesis in red pepper shoots.

Effects of Potato Protein on the Growth of Clostridium perfiringens and Other Intestinal Microorganisms (감자 단백질이 Clostridium perfringens 및 주요 장내 미생물의 생육에 미치는 영향)

  • 신현경;신옥호;구영조
    • Microbiology and Biotechnology Letters
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    • v.20 no.3
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    • pp.249-256
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    • 1992
  • Potato juice was found out to have a strong inhibition activity on the growth of Clostridium perf;nngens during work of foodstuffs for the improvement of human intestinal microflora. The anti-bacterial activity of the precipitated protein obtained from the potato juice in 70% ammonium sulfate solution was stable at the range of pH 4 to 10, whereas it was lost by a heat treatment at $60^{\circ}C$ for 10 min. The minimal inhibitory concentration of the precipitated protein on the growth of C1. Pefingens was about 0.2 mg/ml. The potato protein also suppressed the growth of C1. butyrincm and Eubacterium iimosum, while it showed a promoting effect for the growth of Bifdobacterium bifidum, Bif: animalis, Lactobacillus plantarum and Lact. acidophitus. The potato protein was further purified by CM-Sepharose ion exchange column chromatography, Sephadex G-150 gel filtration column chromatography and SDS-polyacrylarnide gel electrophoresis. The purified protein(kCp) was proved to be a glycoprotein by PAS staining and its molecular weight was about 38.7 kd.

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BolA Affects Cell Growth, and Binds to the Promoters of Penicillin-Binding Proteins 5 and 6 and Regulates Their Expression

  • Guinote, Ines Batista;Matos, Rute Goncalves;Freire, Patrick;Arraiano, Cecilia Maria
    • Journal of Microbiology and Biotechnology
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    • v.21 no.3
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    • pp.243-251
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    • 2011
  • The gene bolA was discovered in the 80's, but unraveling its function in the cell has proven to be a complex task. The BolA protein has pleiotropic effects over cell physiology, altering growth and morphology, inducing biofilm formation, and regulating the balance of several membrane proteins. Recently, BolA was shown to be a transcription factor by repressing the expression of the mreB gene. The present report shows that BolA is a transcriptional regulator of the dacA and dacC genes, thus regulating both DD-carboxypeptidases PBP5 and PBP6 and thereby demonstrating the versatility of BolA as a cellular regulator. In this work, we also demonstrate that reduction of cell growth and survival can be connected to the overexpression of the bolA gene in different E. coli backgrounds, particularly in the exponential growth phase. The most interesting finding is that overproduction of BolA affects bacterial growth differently depending on whether the cells were inoculated directly from a plate culture or from an overnight batch culture. This strengthens the idea that BolA can be engaged in the coordination of genes that adapt the cell physiology in order to enhance cell adaptation and survival under stress conditions.

Growth of Stahylococcus aureus with Defective Siderophore Production in Human Peritoneal Dialysate Solution

  • Park, Ra-Young;Sun, Hui-Yu;Choi, Mi-Hwa;Bae, Young-Hoon;Shin, Sung-Heui-
    • Journal of Microbiology
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    • v.43 no.1
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    • pp.54-61
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    • 2005
  • In this study, we attempted to determine the effects of iron-availability and the activity of the bacterial iron-uptake system (IUS) on the growth of Staphylococcus aureus in human peritoneal dialysate (HPD) solution. A streptonigrin-resistant S. aureus (SRSA) strain, isolated from S. aureus ATCC 6538, exhibited defective siderophore production, thereby resulting in ineffective uptake of iron from low iron-saturated transferrin. The growth of both strains was stimulated in HPD solution supplemented with FeCl_3 and holotransferrin, but growth was inhibited in HPD solution which had been supplemented with apotransferrin and dipyridyl. The SRSA strain grew less robustly than did its parental strain in both iron-supplemented HPD solution and regular HPD solution. These results indicate that iron-availability and siderophore-mediated IUS activity in particular, the ability to produce siderophores and thus capture iron from low iron-saturated transferrin play critical roles in the growth of S. aureus in HPD solution. Our results also indicated that the possibility of using iron chelators as therapeutic or preventive agents warrants further evaluation.

Isolation and Characterization of the Plant Growth Promoting Rhizobacterium, Arthrobacter scleromae SYE-3 on the Yam Growth (식물성장촉진근권미생물 Arthrobacter scleromae SYE-3의 분리 및 Yam (Dioscorea japonica Thunb.) 성장에 미치는 영향 연구)

  • Hong, Sun Hwa;Kim, Ji Seul;Sim, Jun Gyu;Lee, Eun Young
    • KSBB Journal
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    • v.31 no.1
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    • pp.58-65
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    • 2016
  • In this study, Arthrobacter scleromae SYE-3, which was isolated from indigenous plant in a subtropical region, Neigeria, with plant growth promoting activity was evaluated to determine the optimal culture condition. A bacterial strain SYE-3 had the IAA productivity ($89.15{\pm}0.36mg/L$) and ACC deaminase activity ($0.20{\pm}0.06$ at 72 hours). Also, optimal culture conditions such as temperature and pH of strain SYE-3 were $20^{\circ}C$ and 10 in LB medium, respectively. Strain SYE-3 had up to 3% salt tolerance in the LB medium. Plant growth promoting ability of strain SYE-3 using yam (Dioscorea japonica Thunb.) was evaluated. As a result, strain SYE-3 had showed very powerful effect on the increase of the shoot length and root biomass of yam (190.0% and 282.41% increase for 112 days, respectively). These results indicated that Arthrobacter scleromae SYE-3 can serve as a promising microbial resource for the biofertilizers of subtropical crops.

The Promoting Effect of Cornus officinalis fermented with Lactobacillus rhamnosus on Hair Growth (산수유 유산균 발효액의 모발성장 효과)

  • Park, Jang-Soon;Lee, Jae-Sug
    • Korean Journal of Pharmacognosy
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    • v.42 no.3
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    • pp.260-264
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    • 2011
  • The bacterial growth and pH of Cornus officinalis fermented with Lactobacillus rhamnosus during fermentation were evaluated. As the results, the number of the fermentation after fermentation always remained higher than 6 log CFU/mL and the pH of those ranged from 4 to 6. To evaluate the effect of Cornus officinalis fermented with Lactobacillus rhamnosus on hair growth promotion in C57BL/6 mice, Six weeks old male mice were divided into four groups including normal group (saline), negative control group (essence base), positive control group (minoxidil) and experimental group (Cornus officinalis and animal milk fermented with Lactobacillus rhamnosus mixed in negative control). And they were applied topically with test materials for 8 days. Hair regrowth effect in experimental group using gross and histological examination was higher than that in positive control group. Body weight and food intake of four groups didn't show significant difference. These results indicated that the Cornus officinalis fermented with Lactobacillus rhamnosus can be used practically for hair growth or prevention of hair loss.

Effects of Virious Plant Growth Promoting Rhizobacteria on the Growth of Hydroponically Grown Cucumber Plants in Rockwool and Cocopeat Culture (수종의 식물생장촉진 근권세균이 암면과 코코피트경 오이의 생장에 미치는 영향)

  • Cho, Ja-Yong;Chi, Yeon-Tae;Chung, Soon-Ju
    • Korean Journal of Organic Agriculture
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    • v.7 no.1
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    • pp.105-113
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    • 1998
  • This study was conducted to clarify the effects of various rhizobacteria in the root zone in terms of Azospirillum sp., Rhodopseudomonas sp., Pseudomonas sp., fusant of Bacillus sp. and Corynebacterium glutamicum on the growth of hydroponically grown cucumber plants. Densities in bacterial cells of fusant of Bacillus sp. and Corynebacterium glutamicum at different substrates were in the order of cocopeat > rockwool > nutrient solutions at 4 days after bacterialization. Plant growth promoting effects of the various rhizobacteria on the growth of hydroponically grown cucumber plants were in the order of Azospirillum sp. > Rhodopseudomonas sp. $\ge$ fusant of Bacillus sp. and Corynebacterium glutamicum > Pseudomonas sp. > control.

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