• The Plant Pathology Journal
• /
• 제34권5호
• /
• pp.393-402
• /
• 2018

Rice world production is affected due to the growing impact of diseases such as bacterial panicle blight, produced by Burkholderia glumae. The pathogen-induced symptoms include seedling rot, grain rot and leafsheath browning in rice plants. It is currently recognized the entrance of this pathogen to the plant, from infected seeds and from environmental sources of the microorganism. However, it is still not fully elucidated the dynamics and permanence of the pathogen in the plant, from its entry until the development of disease symptoms in seedlings or panicles. In this work it was evaluated the infection of B. glumae rice plants, starting from inoculated seeds and substrates, and its subsequent monitoring after infection. Various organs of the plant during the vegetative stage and until the beginning of the reproductive stage, were evaluated. In both inoculation models, the bacteria was maintained in the plant as an endophyte between $1{\times}10^1$ and $1{\times}10^5cfu$ of B. $glumae.g^{-1}$ of plant throughout the vegetative stage. An increase of bacterial population towards initiation of the panicle was observed, and in the maturity of the grain, an endophyte population was identified in the flag leaf at $1{\times}10^6cfu$ of B. $glumae.g^{-1}$ fresh weight of rice plant, conducting towards the symptoms of bacterial panicle blight. The results found, suggest that B. glumae in rice plants developed from infected seeds or from the substrate, can colonize seedlings, establishing and maintaining a bacterial population over time, using rice plants as habitat to survive endophyticly until formation of bacterial panicle blight symptoms.

• 한국식물병리학회:학술대회논문집
• /
• 한국식물병리학회 2004년도 The 2004 KSPP Annual Meeting & International Symposium
• /
• pp.29-30
• /
• 2004

See Full Text

• 한국농림기상학회:학술대회논문집
• /
• 한국농림기상학회 2005년도 추계 학술발표논문집
• /
• pp.194-196
• /
• 2005

• 한국식물병리학회:학술대회논문집
• /
• 한국식물병리학회 1999년도 정기총회 및 춘계 학술발표회
• /
• pp.45.2-45
• /
• 1999

• 식물병연구
• /
• 제10권3호
• /
• pp.167-174
• /
• 2004

세균성벼알마름병(RGBR)의 발생예찰 모델을 만들기 위하여 출수기가 다른 오대벼 등 21개 품종을 1998년 5월 30일과 6월 15일에 이앙하여 벼 출수기와 이병수율을 조사하고 출수기 전후의 미기상과 병 발생과의 관계를 SAS 프로그램을 이용하여 분석하였다. 세균성벼알름병은 출수기가 7월 29일부터 8월 19일 사이의 품종에서 대부분 발생하였고 8월 22일 이후에 출수한 품종에서는 전혀 발생하지 않았다. 벼 출수기를 기준으로 출수기 3일 전부터 7일(r=-0.871**), 10일(r=-0.863**)의 일교차와 15일(r=0.8709**)과 출수기부터 7일(r=0.862**), 10일(r=0.860**), 15일(r =0.844**) 동안의 상대습도와 높은 상관관계를 보였다. 결정계수($R^2$)와 수정된 결정계수($R^2$$_{adj}$), 잔차평균제곱(MSE)를 이용하여 예찰모델을 구한 결과, 출수 전 3일부터 7일 동안의 평균기온, 최저기온, 평균상대습도(RHavr), 최저상대습도(RHmin), 강우일수, 풍속의 6개 변수를 이용한 RGBR =92.83 - 2.437avr + 1.887min - 1.04RHavr + 0.37RHmin + 0.43RD - 3.68WS($R^2$=0.824)의 회귀식을 구할 수 있었다.다.

• The Plant Pathology Journal
• /
• 제32권6호
• /
• pp.575-579
• /
• 2016

Burkholderia glumae (bacterial grain rot), Xanthomonas oryzae pv. oryzae (bacterial leaf blight), and Acidovorax avenae subsp. avenae (bacterial brown stripe) are major seedborne pathogens of rice. Based on the 16S and 23S rDNA sequences for A. avenae subsp. avenae and B. glumae, and transposase A gene sequence for X. oryzae pv. oryzae, three sets of primers had been designed to produce 402 bp for B. glumae, 490 bp for X. oryzae, and 290 bp for A. avenae subsp. avenae with the $63^{\circ}C$ as an optimum annealing temperature. Samples collected from naturally infected fields were detected with two bacteria, B. glumae and A. avenae subsp. avenae but X. oryzae pv. oryzae was not detected. This assay can be used to identify pathogens directly from infected seeds, and will be an effective tool for the identification of the three pathogens in rice plants.

• 식물병연구
• /
• 제19권3호
• /
• pp.177-182
• /
• 2013

Resvestrol has been known to inhibit bacterial and fungal growth in vitro, and can be accumulated in plant to concentrations necessary to inhibit microbial pathogens. Hence, stilbene synthase gene has been used to transform to synthesize resveratrol in heterologous plant species to enhance resistance against pathogens. In the present study, we investigated the antimicrobial activities of resveratrol and piceid to bacterial and fungal pathogens, which causing severe damages to rice plants. In addition, disease resistance was compared between transgenic rice varieties, Iksan 515 and Iksan 526 transformed with stlibene synthase gene and non-transgenic rice varieties, Dongjin and Nampyeong. Minimum inhibitory concentration of resveratrol for Burkolderia glumae was 437.5 ${\mu}M$, and the mycelial growth of Biplaris oryzae was slightly inhibited at concentration of 10 ${\mu}M$. However, other bacterial and fungal pathogens are not inhibited by resveratrol and piceid. The expression of the stilbene synthase gene in Iksan 515 and Iksan 526 did not significantly enhanced resistance against bacterial grain rot, bacterial leaf blight, sheath blight, and leaf blight. This study is the first report on the effect of resveratrol and piceid against pathogens of rice plant, and changes of disease resistance of transgenic rice plants transformed with stilbene synthase gene.

• 한국식물병리학회:학술대회논문집
• /
• 한국식물병리학회 2002년도 총회 및 추계학술발표회
• /
• pp.46.3-47
• /
• 2002

• 한국식물병리학회:학술대회논문집
• /
• 한국식물병리학회 2002년도 총회 및 추계학술발표회
• /
• pp.42.1-42
• /
• 2002

• 식물병연구
• /
• 제12권3호
• /
• pp.153-157
• /
• 2006

2005년은 전년에 비해 기온이 낮았고 강수량이 적었다. 벼 흰잎마름병, 세균성벼알마름병, 벼 깨씨무늬병, 벼 이삭도열병, 배나무 겹무늬병 등이 2004년에 비해 증가하였다. 특히 벼 깨씨무늬병은 전년에 비해 4배 이상 병 발생이 증가하였다. 벼 이삭도열병이 전년 0.7%에서 2005년 2%로 증가된 것은 출수기 동안 평균기온은 높았고 강수일수, 강수량 및 습도가 높아 잎도열병균이 이삭으로의 전파를 촉진한 것으로 추정되었다. 기타 조사된 병들은 전년에 비해 감소 혹은 유사한 수준이었다. 벼 잎도열병, 시설참외 시들음증상, 흰가루병 및 노균병은 전년에 비해 크게 감소되었다.